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The map label for this gene is yfbB [H]

Identifier: 218548286

GI number: 218548286

Start: 921666

End: 922424

Strand: Direct

Name: yfbB [H]

Synonym: EFER_0905

Alternate gene names: 218548286

Gene position: 921666-922424 (Clockwise)

Preceding gene: 218548285

Following gene: 218548287

Centisome position: 20.09

GC content: 56.52

Gene sequence:

>759_bases
ATGATCCTGCACGCGCAGGCAAAACACGGCAAACCAGGTTTACCCTGGCTGGTGTTTTTGCACGGTTTTTCCGGCGATTG
CCATGAATGGCAAGAAGTGGGCGAGGCGTTTGCCGACTACTCGCGGTTGTATGTTGATCTCCCAGGTCACGGTGGTTCGG
CAGCAATTAGCGTCGATGGATTTGATGATGTCACCGGCTTACTGCGTAAAACTCTGGTTAGTTACAACATCCTTAACTTC
TGGCTGGTGGGGTACTCGCTTGGCGGACGGGTGGCGATGATGGCGGCTTGTCAGGGGATGCCGGGGCTTTGTGGCGTGAT
TGTCGAAGGTGGGCATCCCGGGCTGCAAAGTGCTGAACAACGAGCGGAACGCCAGCTTTCCGATCGCCAGTGGGCGCAGC
GTTTTCGCTCAGAACCGTTAACGGCGGTGTTTACCGACTGGTATCAACAGCCTGTTTTTGCCTCGCTCAATGACGACCAA
CGCCGGGAGCTGGTGGCGCTGCGCAGCAACAATAACGGCGCAACCCTTGCCGCGATGCTGGAGGCTACTTCTCTCGCCGT
CCAGCCTGATTTACGTGCTAACCTTAGCGCCCGCACATTTGCGTTTTATTATTTATGTGGTGAACGTGACAGCAAATTCC
GCGCTCTGGCGGCGGAACTGGCTGCCGACTGCCATGTCATTCCTCGCGCCGGACATAACACGCACCGGGAAAATCCCGCT
GGCGTAATCGCAAGTCTGGCGCAGATCTTGCGTTTCTGA

Upstream 100 bases:

>100_bases
ACGCCTGGCGCACGCCGACCACCACGGTAATTGAAATGGTGGTTAACGACACCGATGGTGCGCAAACCCTCCAGCAACTG
CTGGCGCAGGTAAGCCATTT

Downstream 100 bases:

>100_bases
CTAAAGGGCACAATATGATTTATCCTGATGAAGCAATGCTTTACGCACCGGTTGAATGGCACGACTGCTCCGAAGGTTTC
GAGGACATTCGTTATGAAAA

Product: acyl-CoA thioester hydrolase YfbB

Products: NA

Alternate protein names: SHCHC synthase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLPGHGGSAAISVDGFDDVTGLLRKTLVSYNILNF
WLVGYSLGGRVAMMAACQGMPGLCGVIVEGGHPGLQSAEQRAERQLSDRQWAQRFRSEPLTAVFTDWYQQPVFASLNDDQ
RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNTHRENPA
GVIASLAQILRF

Sequences:

>Translated_252_residues
MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLPGHGGSAAISVDGFDDVTGLLRKTLVSYNILNF
WLVGYSLGGRVAMMAACQGMPGLCGVIVEGGHPGLQSAEQRAERQLSDRQWAQRFRSEPLTAVFTDWYQQPVFASLNDDQ
RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNTHRENPA
GVIASLAQILRF
>Mature_252_residues
MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLPGHGGSAAISVDGFDDVTGLLRKTLVSYNILNF
WLVGYSLGGRVAMMAACQGMPGLCGVIVEGGHPGLQSAEQRAERQLSDRQWAQRFRSEPLTAVFTDWYQQPVFASLNDDQ
RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNTHRENPA
GVIASLAQILRF

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family [H]

Homologues:

Organism=Escherichia coli, GI1788598, Length=252, Percent_Identity=95.6349206349206, Blast_Score=499, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =4.2.99.20 [H]

Molecular weight: Translated: 27659; Mature: 27659

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLPGHGGSAAISVDG
CEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEECC
FDDVTGLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPGLCGVIVEGGHPGLQSAEQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
RAERQLSDRQWAQRFRSEPLTAVFTDWYQQPVFASLNDDQRRELVALRSNNNGATLAAML
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCHHHEEEEEEEECCCCCHHHHHH
EATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNTHRENPA
HHHHHEECCCHHCCCCHHEEEEEEEECCCCCHHHHHHHHHHHCCEEECCCCCCCCCCCHH
GVIASLAQILRF
HHHHHHHHHHCC
>Mature Secondary Structure
MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLPGHGGSAAISVDG
CEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEECC
FDDVTGLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPGLCGVIVEGGHPGLQSAEQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
RAERQLSDRQWAQRFRSEPLTAVFTDWYQQPVFASLNDDQRRELVALRSNNNGATLAAML
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCHHHEEEEEEEECCCCCHHHHHH
EATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNTHRENPA
HHHHHEECCCHHCCCCHHEEEEEEEECCCCCHHHHHHHHHHHCCEEECCCCCCCCCCCHH
GVIASLAQILRF
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA