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The map label for this gene is fadI [H]

Identifier: 218548204

GI number: 218548204

Start: 837734

End: 839044

Strand: Direct

Name: fadI [H]

Synonym: EFER_0822

Alternate gene names: 218548204

Gene position: 837734-839044 (Clockwise)

Preceding gene: 218548203

Following gene: 218548205

Centisome position: 18.26

GC content: 53.01

Gene sequence:

>1311_bases
ATGGGGCAGGTTTTACCGCTGGTCACCCGCCAGGGCGATCGTATCGCCATTGTTAGTGGATTACGTACGCCATTCGCGCG
TCAGGCGACAGCTTTTCATGGCATTCCCGCTGTTGATCTGGGAAAAATGGTGGTAAGTGAACTACTTGCTCGTAGTGAAA
TTCCTCCTGCTGCGATTGAACAACTGGTGTTTGGTCAGGTGGTACAAATGCCGGAAGCCCCCAACATCGCACGTGAAATT
GTGTTGGGCACAGGGATGAGTGTTCATACCGATGCATACAGCGTCAGCCGCGCTTGCGCGACCAGCTTCCAGGCGGTGGC
AAATGTTGCTGAAAGCCTGATGGCAGGAACCATACGTGCAGGTATCGCCGGAGGAGCTGACTCTTCTTCAGTATTGCCTA
TTGGCGTCAGCAAGAAGCTGGCGCGGGTATTAGTTGATGTTAATAAAGCCAGAACGCTGGGCCAGCGGCTGAAATTATTT
TCTCGTCTGCGTCTTCACGACTTATTGCCTGTACCGCCTGCGGTTGCCGAATACTCCACTGGCTTACGTATGGGCGATAC
CGCTGAGCAAATGGCAAAAACTTATGGCATTACCCGTGAACAGCAGGATGCATTGGCACATCGTTCGCATCAACGAGCAG
CTCAAGCGTGGAGCGAAGGCAAACTGACGAATGAGGTGATGACAGCTTATGTTCCGCCTTATAAAGCGCCATTGAGTGAA
GATAACAATATTCGTGGTAATTCCACACTTGCCGATTATGCCCGCTTGCGCCCGGCATTTGACCGTAAGCATGGCACGGT
AACCGCAGCGAACAGTACGCCACTGACGGATGGCGCAGCTGCAGTCATTATGATGACAGAAAGCCGGGCAAAAGAACTTG
GACTAACACCGTTGGGATATTTACGCAGCTATGCTTTTACAGCCATTGATGTCTGGCAGGACATGTTGCTCGGCCCGGCA
TGGTCGACACCACTGGCACTGGATCGTGCAGGACTGACACTGGCAGATCTTACACTGATTGATATGCATGAAGCCTTTGC
AGCGCAGACGTTGGCCAATGTTCAGTTGCTCGGTAGTGAACGTTTCGCCCGCGATGTGTTGGGGCGCGCTCATGCGACAG
GAGAGGTCGATGACAGTAAATTTAATGTGCTGGGGGGCTCCATCGCTTATGGTCATCCTTTTGCCGCTACCGGCGCACGA
ATGATTACCCAAACGCTCCATGAACTTCGTCGTCGAGGCGGCGGTTTCGGGTTGGTGACAGCCTGTGCTGCTGGTGGTTT
AGGTGCAGCAATGGTTCTGGAGGCGGAATAA

Upstream 100 bases:

>100_bases
GCTCTCAATTTCATTTATCTTCGCTTGGCTAAATGTAAAAAATTAGTTAAGACTATGATCAGGTCCGACCACTTAATTAT
AATTTTTACAGGGGATTGTT

Downstream 100 bases:

>100_bases
TGGACATGACATCAGCATTTACTCTTAATGTACGCCTGGACCACATTGCTGTGGTAACTATTGACGTTCCCGGCGAAAAG
ATGAACACCCTCAAGGCTGA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVSELLARSEIPPAAIEQLVFGQVVQMPEAPNIAREI
VLGTGMSVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTLGQRLKLF
SRLRLHDLLPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSEGKLTNEVMTAYVPPYKAPLSE
DNNIRGNSTLADYARLRPAFDRKHGTVTAANSTPLTDGAAAVIMMTESRAKELGLTPLGYLRSYAFTAIDVWQDMLLGPA
WSTPLALDRAGLTLADLTLIDMHEAFAAQTLANVQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGAR
MITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE

Sequences:

>Translated_436_residues
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVSELLARSEIPPAAIEQLVFGQVVQMPEAPNIAREI
VLGTGMSVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTLGQRLKLF
SRLRLHDLLPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSEGKLTNEVMTAYVPPYKAPLSE
DNNIRGNSTLADYARLRPAFDRKHGTVTAANSTPLTDGAAAVIMMTESRAKELGLTPLGYLRSYAFTAIDVWQDMLLGPA
WSTPLALDRAGLTLADLTLIDMHEAFAAQTLANVQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGAR
MITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE
>Mature_435_residues
GQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVSELLARSEIPPAAIEQLVFGQVVQMPEAPNIAREIV
LGTGMSVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTLGQRLKLFS
RLRLHDLLPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSEGKLTNEVMTAYVPPYKAPLSED
NNIRGNSTLADYARLRPAFDRKHGTVTAANSTPLTDGAAAVIMMTESRAKELGLTPLGYLRSYAFTAIDVWQDMLLGPAW
STPLALDRAGLTLADLTLIDMHEAFAAQTLANVQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARM
ITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=40.4705882352941, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI4557237, Length=424, Percent_Identity=29.7169811320755, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI4501853, Length=430, Percent_Identity=32.5581395348837, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI167614485, Length=424, Percent_Identity=31.1320754716981, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI148539872, Length=427, Percent_Identity=29.9765807962529, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI194353979, Length=258, Percent_Identity=27.1317829457364, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1788683, Length=436, Percent_Identity=94.0366972477064, Blast_Score=819, Evalue=0.0,
Organism=Escherichia coli, GI1788554, Length=420, Percent_Identity=37.1428571428571, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI87082165, Length=422, Percent_Identity=33.175355450237, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI48994986, Length=436, Percent_Identity=34.1743119266055, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1787663, Length=428, Percent_Identity=32.4766355140187, Blast_Score=179, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17551802, Length=422, Percent_Identity=38.8625592417062, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI133906874, Length=425, Percent_Identity=31.7647058823529, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI25147385, Length=420, Percent_Identity=28.5714285714286, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17535921, Length=426, Percent_Identity=28.8732394366197, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17535917, Length=427, Percent_Identity=23.4192037470726, Blast_Score=92, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6325229, Length=433, Percent_Identity=30.0230946882217, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6322031, Length=447, Percent_Identity=28.1879194630872, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17137578, Length=430, Percent_Identity=41.3953488372093, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24655093, Length=422, Percent_Identity=29.6208530805687, Blast_Score=185, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24640423, Length=426, Percent_Identity=27.4647887323944, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI17648125, Length=425, Percent_Identity=29.4117647058824, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 46308; Mature: 46176

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVSELLARSEIPPAAIE
CCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMSVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
HHHHHHHHCCCCCCCHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAGGADSSSVLPIGVSKKLARVLVDVNKARTLGQRLKLFSRLRLHDLLPVPPAVAEYST
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSEGKLTNEVMTAYVPPYKAPLSE
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
DNNIRGNSTLADYARLRPAFDRKHGTVTAANSTPLTDGAAAVIMMTESRAKELGLTPLGY
CCCCCCCCHHHHHHHHCHHHHCCCCEEEECCCCCCCCCCEEEEEEECHHHHHCCCCHHHH
LRSYAFTAIDVWQDMLLGPAWSTPLALDRAGLTLADLTLIDMHEAFAAQTLANVQLLGSE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCHH
RFARDVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT
HHHHHHHHHHCCCCCCCCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMVLEAE
HHHCCCCCEEEEEECC
>Mature Secondary Structure 
GQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVSELLARSEIPPAAIE
CCCCCHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMSVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
HHHHHHHHCCCCCCCHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAGGADSSSVLPIGVSKKLARVLVDVNKARTLGQRLKLFSRLRLHDLLPVPPAVAEYST
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSEGKLTNEVMTAYVPPYKAPLSE
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
DNNIRGNSTLADYARLRPAFDRKHGTVTAANSTPLTDGAAAVIMMTESRAKELGLTPLGY
CCCCCCCCHHHHHHHHCHHHHCCCCEEEECCCCCCCCCCEEEEEEECHHHHHCCCCHHHH
LRSYAFTAIDVWQDMLLGPAWSTPLALDRAGLTLADLTLIDMHEAFAAQTLANVQLLGSE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCHH
RFARDVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT
HHHHHHHHHHCCCCCCCCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMVLEAE
HHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA