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The map label for this gene is benA [H]

Identifier: 218548181

GI number: 218548181

Start: 816522

End: 817823

Strand: Direct

Name: benA [H]

Synonym: EFER_0798

Alternate gene names: 218548181

Gene position: 816522-817823 (Clockwise)

Preceding gene: 218548180

Following gene: 218548182

Centisome position: 17.79

GC content: 57.3

Gene sequence:

>1302_bases
ATGCAGTGTGATAACCAGATCCCCGTTAAAAATATCGAAGCGAATGTTCCCGACTGCGCGGAAATCGCCGCGCTGGTGCA
GTCTGACCGGGTGCATACCTCACTTTATAATTCCGAGCCCCTGTTCAACCTTGAGCTGGACAGGATCTTTAGCAAAACCT
GGGTATGGGTGGCGCATACCAGCGAAATCCCGGAAACCGGCAGTTTCAAAACCACCGAGATCGGCACGCAGCCGGTGATC
GTCGTGCGCGACCGCAAAGGCAACGTGCATACGCTGCTCAACCGTTGCCGTCACCGCGCCGCCACCGTGTGCGAACACCG
CACCGGCAAAACCAACAGTTTTGTCTGCCCGTATCACGGCTGGGGCTACGCGCTGGACGGCAGCCTGCGCGGCGTGCCGC
ACCCGGAAAGCTATGCCGATCAACTGGAAAAAGGCGAGCTGGGGCTGGTGTCGCTGCGCACCGAACAGTACGCCGGCATG
ATCTTCGCCACCTTTAATGAGCACATCGAACCGCTGGAAGATTTCCTCGGTGCGGCGAAAAAGTGGATGGATCTGTTTAT
GAAGCAGGGCGCGGGTTATCCGATTAAAACCGGCCCGGCGCACCGTTTCCGCTTCCCCGGCAACTGGAAGATCCAACTGG
AAAATACCACCGACGGCTATCACTTCCCGGTTGTTCACCGCTCGTTTTTAAGTTCGGTCGATAAGCAGACCGAAGAGATG
CTCAACTTTGTTGACGGCAGCGGCTATGTGGAAGATCTCGGCAACGGTCACAGCGTGATGGTAATGATCCCGGAACTGGT
GGATCTGGAAGCTAATCTTGATGCGCCAATCCCGGAGCGATTTGTTGCGTTGGCCGACGAATTGCGTAAAGACCACGATG
AAGATCAGGTACGTCGCATTGTGCGTGCAGTCGGCGGTTCCGGCTTTAACCTCAACATTTTCCCCAATATTGCCTGTTCA
ATGGCCTTCTTCCGCGTGCTGCAACCTGTCTCGGTGCATGAAACTGAAATTCACCATGCGGTGATCACCATGGACGGCGG
CCCGGAAATTGCCAACCAGGCACGTCTTCGTCTGCATGAACACTTCCAGGGGCCGATGGGTTTTGGCACGCCGGATGACT
CCGAGGCGTGGGAGCGCGTGCAGCGCGGGGCAACGGCGGGCAACGACCTGTGGATCATGCTTAACCGCGGGCTGGCGGGT
GAATACCGCAGTGATGACGGCCTGCGTAGCGACGTGAGCGCTGAAACGGGGATGCGCGCGGCGTATCAACAGTGGAAAAA
ACTGATGACGGAGGCGGAATAA

Upstream 100 bases:

>100_bases
TGCACCATTTCACGACACGCAATCTGTTCAGCAGGCCAGTGGACCACTGGCACGCCGCAGATGTGCTGCCTGTACCTGAC
AATAAATAGAGAGTCGAGCG

Downstream 100 bases:

>100_bases
TGACGCCAGATACGGTTCTGATTTCCGCAATGGAAATAATCAATCTCGAAGGCGACTTGCTCGATCAGGGCGAGTTCAAT
GCCTGGCTGGAGCTGTGGCA

Product: ring hydroxylating dioxygenase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MQCDNQIPVKNIEANVPDCAEIAALVQSDRVHTSLYNSEPLFNLELDRIFSKTWVWVAHTSEIPETGSFKTTEIGTQPVI
VVRDRKGNVHTLLNRCRHRAATVCEHRTGKTNSFVCPYHGWGYALDGSLRGVPHPESYADQLEKGELGLVSLRTEQYAGM
IFATFNEHIEPLEDFLGAAKKWMDLFMKQGAGYPIKTGPAHRFRFPGNWKIQLENTTDGYHFPVVHRSFLSSVDKQTEEM
LNFVDGSGYVEDLGNGHSVMVMIPELVDLEANLDAPIPERFVALADELRKDHDEDQVRRIVRAVGGSGFNLNIFPNIACS
MAFFRVLQPVSVHETEIHHAVITMDGGPEIANQARLRLHEHFQGPMGFGTPDDSEAWERVQRGATAGNDLWIMLNRGLAG
EYRSDDGLRSDVSAETGMRAAYQQWKKLMTEAE

Sequences:

>Translated_433_residues
MQCDNQIPVKNIEANVPDCAEIAALVQSDRVHTSLYNSEPLFNLELDRIFSKTWVWVAHTSEIPETGSFKTTEIGTQPVI
VVRDRKGNVHTLLNRCRHRAATVCEHRTGKTNSFVCPYHGWGYALDGSLRGVPHPESYADQLEKGELGLVSLRTEQYAGM
IFATFNEHIEPLEDFLGAAKKWMDLFMKQGAGYPIKTGPAHRFRFPGNWKIQLENTTDGYHFPVVHRSFLSSVDKQTEEM
LNFVDGSGYVEDLGNGHSVMVMIPELVDLEANLDAPIPERFVALADELRKDHDEDQVRRIVRAVGGSGFNLNIFPNIACS
MAFFRVLQPVSVHETEIHHAVITMDGGPEIANQARLRLHEHFQGPMGFGTPDDSEAWERVQRGATAGNDLWIMLNRGLAG
EYRSDDGLRSDVSAETGMRAAYQQWKKLMTEAE
>Mature_433_residues
MQCDNQIPVKNIEANVPDCAEIAALVQSDRVHTSLYNSEPLFNLELDRIFSKTWVWVAHTSEIPETGSFKTTEIGTQPVI
VVRDRKGNVHTLLNRCRHRAATVCEHRTGKTNSFVCPYHGWGYALDGSLRGVPHPESYADQLEKGELGLVSLRTEQYAGM
IFATFNEHIEPLEDFLGAAKKWMDLFMKQGAGYPIKTGPAHRFRFPGNWKIQLENTTDGYHFPVVHRSFLSSVDKQTEEM
LNFVDGSGYVEDLGNGHSVMVMIPELVDLEANLDAPIPERFVALADELRKDHDEDQVRRIVRAVGGSGFNLNIFPNIACS
MAFFRVLQPVSVHETEIHHAVITMDGGPEIANQARLRLHEHFQGPMGFGTPDDSEAWERVQRGATAGNDLWIMLNRGLAG
EYRSDDGLRSDVSAETGMRAAYQQWKKLMTEAE

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB) [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=427, Percent_Identity=30.4449648711944, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788103, Length=200, Percent_Identity=29.5, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.10 [H]

Molecular weight: Translated: 48636; Mature: 48636

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCDNQIPVKNIEANVPDCAEIAALVQSDRVHTSLYNSEPLFNLELDRIFSKTWVWVAHT
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCEEEHHHHHHHHEEEEEEEC
SEIPETGSFKTTEIGTQPVIVVRDRKGNVHTLLNRCRHRAATVCEHRTGKTNSFVCPYHG
CCCCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCC
WGYALDGSLRGVPHPESYADQLEKGELGLVSLRTEQYAGMIFATFNEHIEPLEDFLGAAK
CCEEECCCCCCCCCCHHHHHHHHCCCEEEEEEECHHHCCEEEEEHHHHHHHHHHHHHHHH
KWMDLFMKQGAGYPIKTGPAHRFRFPGNWKIQLENTTDGYHFPVVHRSFLSSVDKQTEEM
HHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHHHH
LNFVDGSGYVEDLGNGHSVMVMIPELVDLEANLDAPIPERFVALADELRKDHDEDQVRRI
HHHHCCCCHHHHCCCCCEEEEEECCHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
VRAVGGSGFNLNIFPNIACSMAFFRVLQPVSVHETEIHHAVITMDGGPEIANQARLRLHE
HHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHH
HFQGPMGFGTPDDSEAWERVQRGATAGNDLWIMLNRGLAGEYRSDDGLRSDVSAETGMRA
HCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
AYQQWKKLMTEAE
HHHHHHHHHHCCC
>Mature Secondary Structure
MQCDNQIPVKNIEANVPDCAEIAALVQSDRVHTSLYNSEPLFNLELDRIFSKTWVWVAHT
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCEEEHHHHHHHHEEEEEEEC
SEIPETGSFKTTEIGTQPVIVVRDRKGNVHTLLNRCRHRAATVCEHRTGKTNSFVCPYHG
CCCCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCC
WGYALDGSLRGVPHPESYADQLEKGELGLVSLRTEQYAGMIFATFNEHIEPLEDFLGAAK
CCEEECCCCCCCCCCHHHHHHHHCCCEEEEEEECHHHCCEEEEEHHHHHHHHHHHHHHHH
KWMDLFMKQGAGYPIKTGPAHRFRFPGNWKIQLENTTDGYHFPVVHRSFLSSVDKQTEEM
HHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHHHH
LNFVDGSGYVEDLGNGHSVMVMIPELVDLEANLDAPIPERFVALADELRKDHDEDQVRRI
HHHHCCCCHHHHCCCCCEEEEEECCHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
VRAVGGSGFNLNIFPNIACSMAFFRVLQPVSVHETEIHHAVITMDGGPEIANQARLRLHE
HHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHH
HFQGPMGFGTPDDSEAWERVQRGATAGNDLWIMLNRGLAGEYRSDDGLRSDVSAETGMRA
HCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
AYQQWKKLMTEAE
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]