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The map label for this gene is gatA [H]

Identifier: 218548177

GI number: 218548177

Start: 813253

End: 814383

Strand: Direct

Name: gatA [H]

Synonym: EFER_0794

Alternate gene names: 218548177

Gene position: 813253-814383 (Clockwise)

Preceding gene: 218548176

Following gene: 218548178

Centisome position: 17.72

GC content: 63.57

Gene sequence:

>1131_bases
GTGTCAATCATGAAGCCAGTTTTTGAGAGCAATGGTTTTGTCGAGGAGTTCACGCTCGGCGCGGGCGAACTGCGGTTCGC
CGTCAAAGATACGCTGGATATCGCCGGTCGCCCGACACGCGCCGGATGCCCGGCACTTGCTGACGCGCCGAAAGCGAAAC
AGCATGCCCGCATCGTCAAGGTGCTGCTCAACAGCGGCTGCCAGCTGACGGGCAAAACTACCCTGCACGAACTGGCGTTT
GGCGTCACCGGGATCAACCCGTGGAGCGGCACGCCCGTTAATCCTCAATACCCCGCGCTGATTCCCGGCGGTTCGTCGAG
CGGCTCTGCAACCGTCGTCGCCAGGGGCGATGTCGATTTCGCCCTCGGCACTGACACCGGAGGTTCTGTCCGAATGCCCG
CCGCCTGCTGCGGCGTGCTGGGGCTGAAACCGGGCTATGGCGTGCTCAGCCGACAGGGCGTATTACCTGCCTACAGCTCA
CTGGACTGCGTTGGGATTTTTGCCCGCGAGCCGCGCATTTTACGTGACGTGCTGACCCGGCTGGCGTTACCGGTTGATGC
GCCGCTGGATTCTCTGCCCGCTATCGGTTTTATCTTTGCCGCTCAGCCGGACATTGACGCCCTGCTGGTGGATGCGCTGG
AAGCGTTGGGCATCGCCGCAAACAGCGTGACGCTTCCGCTGCTGGGCGAAGCGCATCGTGCCGGGCTGACCATCATCAAC
CGCGAAAACTGGCTGGCGTTTAATGCGCTGCTGGCGAGCCACGCGGTCTCCCCGGACGTCGCCAGCCGTATTCAGGCCGG
AGAATCCATCACCAGCGACCAGCTCGCCAGTGCGGAAAACGTGCGCGTGCAGTTCACCGGTCAGGTGAATGCCCTGCTAG
AAGATACGCCTCTGCTGGCGCTGGCGACGCTGCCGGAACTGCCGCCGACGCTGGAAGAGGCGGAAGATCCGCTTAGCGTG
GTGAATCTGACGCGGCTGGTGCGCCCCTTCAACCTCAGCGGTCATCCGGCGATCTCGCTGCCGATGGGCGAACTTCAGGG
CCGACCTGTCGCGCTACAGTTGGTGGCAGGCTTTGGCGAAGAGGGGCTTCTCGTCCAGGCCGCCGAGTGGCTGATGCTCC
ACCGCCTCTGA

Upstream 100 bases:

>100_bases
GAATATTCACGTAAATCTCTCATGATTGATTTATCGCCATTCAGGGTAAAAAAGCAGCAATAACTGTTCGCCTGAATGTG
ATTCACTTTTTCTGGTCAAG

Downstream 100 bases:

>100_bases
CGTTTATCGCGCCATAAAACCGTCAAACCGACCACTCAATGCCATTCAGGCGTTGCGGCATCGTGCACCTTTTTGCCGCC
CGCCCACATTATTCGGGAGT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSIMKPVFESNGFVEEFTLGAGELRFAVKDTLDIAGRPTRAGCPALADAPKAKQHARIVKVLLNSGCQLTGKTTLHELAF
GVTGINPWSGTPVNPQYPALIPGGSSSGSATVVARGDVDFALGTDTGGSVRMPAACCGVLGLKPGYGVLSRQGVLPAYSS
LDCVGIFAREPRILRDVLTRLALPVDAPLDSLPAIGFIFAAQPDIDALLVDALEALGIAANSVTLPLLGEAHRAGLTIIN
RENWLAFNALLASHAVSPDVASRIQAGESITSDQLASAENVRVQFTGQVNALLEDTPLLALATLPELPPTLEEAEDPLSV
VNLTRLVRPFNLSGHPAISLPMGELQGRPVALQLVAGFGEEGLLVQAAEWLMLHRL

Sequences:

>Translated_376_residues
MSIMKPVFESNGFVEEFTLGAGELRFAVKDTLDIAGRPTRAGCPALADAPKAKQHARIVKVLLNSGCQLTGKTTLHELAF
GVTGINPWSGTPVNPQYPALIPGGSSSGSATVVARGDVDFALGTDTGGSVRMPAACCGVLGLKPGYGVLSRQGVLPAYSS
LDCVGIFAREPRILRDVLTRLALPVDAPLDSLPAIGFIFAAQPDIDALLVDALEALGIAANSVTLPLLGEAHRAGLTIIN
RENWLAFNALLASHAVSPDVASRIQAGESITSDQLASAENVRVQFTGQVNALLEDTPLLALATLPELPPTLEEAEDPLSV
VNLTRLVRPFNLSGHPAISLPMGELQGRPVALQLVAGFGEEGLLVQAAEWLMLHRL
>Mature_375_residues
SIMKPVFESNGFVEEFTLGAGELRFAVKDTLDIAGRPTRAGCPALADAPKAKQHARIVKVLLNSGCQLTGKTTLHELAFG
VTGINPWSGTPVNPQYPALIPGGSSSGSATVVARGDVDFALGTDTGGSVRMPAACCGVLGLKPGYGVLSRQGVLPAYSSL
DCVGIFAREPRILRDVLTRLALPVDAPLDSLPAIGFIFAAQPDIDALLVDALEALGIAANSVTLPLLGEAHRAGLTIINR
ENWLAFNALLASHAVSPDVASRIQAGESITSDQLASAENVRVQFTGQVNALLEDTPLLALATLPELPPTLEEAEDPLSVV
NLTRLVRPFNLSGHPAISLPMGELQGRPVALQLVAGFGEEGLLVQAAEWLMLHRL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=429, Percent_Identity=27.039627039627, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI195972892, Length=399, Percent_Identity=24.812030075188, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=391, Percent_Identity=27.6214833759591, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=131, Percent_Identity=33.587786259542, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=379, Percent_Identity=33.509234828496, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323950, Length=158, Percent_Identity=44.9367088607595, Blast_Score=102, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24648113, Length=315, Percent_Identity=27.6190476190476, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356731, Length=390, Percent_Identity=25.3846153846154, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 39375; Mature: 39244

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIMKPVFESNGFVEEFTLGAGELRFAVKDTLDIAGRPTRAGCPALADAPKAKQHARIVK
CCCCCCHHHCCCCEEEEECCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
VLLNSGCQLTGKTTLHELAFGVTGINPWSGTPVNPQYPALIPGGSSSGSATVVARGDVDF
HHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCEE
ALGTDTGGSVRMPAACCGVLGLKPGYGVLSRQGVLPAYSSLDCVGIFAREPRILRDVLTR
EEECCCCCCEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHH
LALPVDAPLDSLPAIGFIFAAQPDIDALLVDALEALGIAANSVTLPLLGEAHRAGLTIIN
HCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEE
RENWLAFNALLASHAVSPDVASRIQAGESITSDQLASAENVRVQFTGQVNALLEDTPLLA
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHCCCCCEEEEEECCEEEECCCCCEEE
LATLPELPPTLEEAEDPLSVVNLTRLVRPFNLSGHPAISLPMGELQGRPVALQLVAGFGE
EECCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCCCEEECCHHHHCCCCEEEEEEECCCC
EGLLVQAAEWLMLHRL
CCEEHHHHHHHHHHCC
>Mature Secondary Structure 
SIMKPVFESNGFVEEFTLGAGELRFAVKDTLDIAGRPTRAGCPALADAPKAKQHARIVK
CCCCCHHHCCCCEEEEECCCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
VLLNSGCQLTGKTTLHELAFGVTGINPWSGTPVNPQYPALIPGGSSSGSATVVARGDVDF
HHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCEE
ALGTDTGGSVRMPAACCGVLGLKPGYGVLSRQGVLPAYSSLDCVGIFAREPRILRDVLTR
EEECCCCCCEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHH
LALPVDAPLDSLPAIGFIFAAQPDIDALLVDALEALGIAANSVTLPLLGEAHRAGLTIIN
HCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEE
RENWLAFNALLASHAVSPDVASRIQAGESITSDQLASAENVRVQFTGQVNALLEDTPLLA
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHCCCCCEEEEEECCEEEECCCCCEEE
LATLPELPPTLEEAEDPLSVVNLTRLVRPFNLSGHPAISLPMGELQGRPVALQLVAGFGE
EECCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCCCEEECCHHHHCCCCEEEEEEECCCC
EGLLVQAAEWLMLHRL
CCEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]