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The map label for this gene is ligA [H]

Identifier: 218548148

GI number: 218548148

Start: 777750

End: 779765

Strand: Direct

Name: ligA [H]

Synonym: EFER_0763

Alternate gene names: 218548148

Gene position: 777750-779765 (Clockwise)

Preceding gene: 218548147

Following gene: 218548149

Centisome position: 16.95

GC content: 54.22

Gene sequence:

>2016_bases
ATGGAATCAATCGAACAACAACTGACAGAACTGCGAACTACGCTTCGCCATCATGAATATCTTTATCATGTGATGGATGC
GCCGGAAATTCCCGACGCTGAATATGACAGGCTGATGCGTGAACTGCGCGAGCTGGAAACCAAACATCCAGAACTAATTA
CGCCTGATTCGCCTACCCAACGTGTAGGTGCAGCGCCGCTGGCGGCTTTCAGCCAGATCCGCCATGAAGTGCCAATGCTG
TCGCTGGATAACGTTTTTGATGAAGAAAGCTTTCTTGCTTTCAACAAACGTGTGCAGGACCGTCTGAAAAGCAACGAGAA
AGTCACCTGGTGTTGTGAGCTGAAGCTGGATGGTCTTGCCGTCAGCATTCTGTATGAAAATGGCGTTTTAGTCAGTGCAG
CGACCCGTGGCGATGGCACTACCGGCGAAGACATTACATCAAACGTGCGTACTATTCGCGCCATTCCGCTGAAGCTTCAC
GGCGAGAATATCCCGGCGCGTCTGGAAGTGCGTGGTGAAGTGTTCCTGCCGCAGGCGGGCTTCGAAAAGATCAACGAAGA
TGCGCGACGCACAGGCGGGAAAGTGTTTGCTAACCCGCGTAATGCGGCTGCAGGTTCACTGCGTCAGCTTGATCCGCGTA
TTACAGCGAAGCGACCGCTCACTTTTTTCTGCTATGGCGTCGGTGTTCTGGAAGGTGGCGAGCTGCCGGATACTCATCTT
GGCCGTTTACTGCAATTTAAAAAGTGGGGTTTGCCGGTCAGCGATCGAGTAACGCTTTGTGAATCGGCGGAAGAAGTGCT
GGCGTTCTACCACAAAGTGGAAGAAGACCGCCCGACGCTGGGCTTTGATATCGACGGCGTGGTAATTAAGGTCAACTCTC
TGGCACAGCAGGAGCAGCTTGGCTTTGTCGCGCGAGCCCCGCGCTGGGCGGTAGCGTTTAAATTCCCGGCGCAGGAACAG
ATGACCTTTGTGCGTGACGTCGAGTTTCAGGTTGGGCGTACTGGCGCGATTACGCCTGTTGCGCGTCTTGAACCAGTCCA
TGTTGCTGGTGTGCTGGTGAGTAACGCCACACTGCATAATGCTGACGAAATCGAACGTCTTGGTTTACGCATTGGCGATA
AAGTGGTGATTCGCCGCGCTGGCGACGTGATCCCGCAGGTGGTTAACGTCGTGCTTTCTGAACGCCCGGAAGATACCCGT
GAGGTTGTATTCCCGACGCATTGCCCGGTATGTGGTTCTGACGTCGAGCGTGTGGAAGGTGAAGCTGTTGCCCGCTGTAC
CGGTGGCCTGATTTGCGGTGCTCAGCGTAAAGAGTCGCTGAAACACTTTGTTTCCCGCCGCGCGATGGATGTTGACGGTA
TGGGCGACAAAATCATCGATCAGCTGGTTGAAAAAGAATATGTCCACACTCCGGCGGATCTGTTCAAACTCACCGCGGGC
AAACTGACCGGACTGGAGCGCATGGGGCCGAAATCGGCACAAAACGTGGTTAACGCGCTGGAAAAAGCGAAAGAAACCAC
CTTTGCCCGTTTCCTTTATGCGCTTGGCATCCGTGAAGTCGGCGAGGCCACTGCAGCAGGTCTGGCGGCATATTTTGGCA
CGCTGGAAGCGCTGGAAGCCGCTTCGATTGAAGAGCTGCAAAAAGTGCCAGATGTTGGAATTGTTGTTGCCTCCCACGTT
CACAACTTCTTTGCCGAAGAAAGCAACCGCAATGTCATCAGCGAGCTGTTGGCGGAAGGTGTTCACTGGCCTGCACCGGT
AGTAATCAACGCGGAAGAGATTGACAGCCCGTTTGCCGGTAAAACCGTGGTGCTTACGGGCAGCTTAAGTCAGATGTCGC
GTGATGACGCTAAAGCTCGACTGGTCGAACTGGGGGCGAAAGTCGCGGGCAGCGTGTCGAAGAAAACCGATCTGGTGATA
GCGGGTGAAGCTGCAGGATCTAAACTGGCGAAGGCGCAGGAACTGGGCATTGAAGTCATCGACGAAACGGAAATGCTGCG
TTTGCTGGGTAGCTGA

Upstream 100 bases:

>100_bases
ATCCGCGAAGTAAAAGCCGCCAACGCCTGATACACTTAAGGCCAATTAACTCCTCTTCGAACCCCCGCTTGTCGGGGGTT
TTTAGCATTGATGGTGCGAT

Downstream 100 bases:

>100_bases
GATGGAAAAAGAGCAGCTGATTGAAATAGCCAATACGATAATGCCGTTTGGCAAGTACAAAGGACGTCGCTTAATCGACC
TGCCGGAAGAGTATCTTTTG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPML
SLDNVFDEESFLAFNKRVQDRLKSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHL
GRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQ
MTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTR
EVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADLFKLTAG
KLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHV
HNFFAEESNRNVISELLAEGVHWPAPVVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVELGAKVAGSVSKKTDLVI
AGEAAGSKLAKAQELGIEVIDETEMLRLLGS

Sequences:

>Translated_671_residues
MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPML
SLDNVFDEESFLAFNKRVQDRLKSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHL
GRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQ
MTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTR
EVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADLFKLTAG
KLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHV
HNFFAEESNRNVISELLAEGVHWPAPVVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVELGAKVAGSVSKKTDLVI
AGEAAGSKLAKAQELGIEVIDETEMLRLLGS
>Mature_671_residues
MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPML
SLDNVFDEESFLAFNKRVQDRLKSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHL
GRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQ
MTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTR
EVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADLFKLTAG
KLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHV
HNFFAEESNRNVISELLAEGVHWPAPVVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVELGAKVAGSVSKKTDLVI
AGEAAGSKLAKAQELGIEVIDETEMLRLLGS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=99.5529061102832, Blast_Score=1362, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=518, Percent_Identity=23.3590733590734, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 73596; Mature: 73596

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCHH
RVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKSNEKVTWCCELKLDGLA
HHCCCHHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEE
VSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGENIPARLEVRGEVFLPQAG
EEEEEECCEEEEEECCCCCCCCHHHHHCCEEEEEEEEEEECCCCCEEEEECCEEECCCCC
FEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHL
HHHHHHHHHHCCCEEEECCCCCHHCCHHHCCCCCCCCCCCEEEEEEEEEECCCCCCHHHH
GRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQEQL
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHC
GFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHN
CHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEEEECCCCCC
ADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTREVVFPTHCPVCGSDVERVEG
HHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHCC
EAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADLFKLTAG
HHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
KLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGTLEALEA
HHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASIEELQKVPDVGIVVASHVHNFFAEESNRNVISELLAEGVHWPAPVVINAEEIDSPFAG
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCC
KTVVLTGSLSQMSRDDAKARLVELGAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVI
CEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCEEE
DETEMLRLLGS
CHHHHHHHHCC
>Mature Secondary Structure
MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCHH
RVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKSNEKVTWCCELKLDGLA
HHCCCHHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEE
VSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGENIPARLEVRGEVFLPQAG
EEEEEECCEEEEEECCCCCCCCHHHHHCCEEEEEEEEEEECCCCCEEEEECCEEECCCCC
FEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHL
HHHHHHHHHHCCCEEEECCCCCHHCCHHHCCCCCCCCCCCEEEEEEEEEECCCCCCHHHH
GRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQEQL
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHC
GFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHN
CHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEEEEECCCCCC
ADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTREVVFPTHCPVCGSDVERVEG
HHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHCC
EAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADLFKLTAG
HHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
KLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGTLEALEA
HHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASIEELQKVPDVGIVVASHVHNFFAEESNRNVISELLAEGVHWPAPVVINAEEIDSPFAG
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCC
KTVVLTGSLSQMSRDDAKARLVELGAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVI
CEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCEEE
DETEMLRLLGS
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA