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The map label for this gene is eutB

Identifier: 218548117

GI number: 218548117

Start: 748564

End: 749925

Strand: Direct

Name: eutB

Synonym: EFER_0729

Alternate gene names: 218548117

Gene position: 748564-749925 (Clockwise)

Preceding gene: 218548116

Following gene: 218548118

Centisome position: 16.31

GC content: 55.36

Gene sequence:

>1362_bases
ATGAAACTAAAGACCACATTGTTCGGCAATGTATATCAGTTTAAGGATGTAAAAGAGGTGCTGGCTAAAGCCAACGAACT
GCGTTCGGGGGATGTGCTGGCGGGCGTTGCAGCGGCAAGTTCACAGGAGCGCGTGGCGGCAAAGCAGGTGTTGTCGGAAA
TGACCGTAGCGGACATCCGCAATAATCCGGTGATTGCCTATGAAGATGACTGCGTTACGCGGCTGATTCAGGACGACGTT
AACGAAACGGCCTACAACCAGATTAAAAACTGGAGCATCAGCGAGCTGCGTGAGTATGTGCTGAGTGATGAAACCAGCGT
TGACGACATTGCTTTTACCCGCAAAGGGCTGACCTCGGAAGTGGTCGCGGCGGTAGCGAAGATTTGCTCTAACGCGGACC
TGATCTACGGCGCGAAGAAAATGCCGGTAATCAAAAAGGCCAATACAACTATTGGTATTCCGGGCACCTTCAGCGCCCGT
TTGCAGCCAAACGATACCCGCGACGACGTGCAAAGTATCGCCGCGCAAATCTACGAAGGGCTTTCTTTCGGGGTGGGCGA
TGCGGTGATCGGCGTTAACCCGGTGACTGACGACGTGGAAAACTTAAGCCGCGTGCTGGATACCATCTATGGCGTGATCG
ACAAATTCAATATTCCAACCCAGGGCTGCGTACTGGCGCACGTCACCACCCAGATCGAAGCGATTCGTCGCGGTGCGCCG
GGCGGGCTGATTTTCCAGAGTATCTGCGGCAGTGAAAAAGGGCTGAAAGAGTTTGGCGTAGAGCTGGCGATGCTCGACGA
AGCGCGCGCAGTGGGCGCGGAGTTCAACCGTATCGCCGGGGAAAACTGCCTCTACTTCGAAACCGGGCAAGGTTCTGCGT
TGTCCGCTGGCGCTAACTTCGGCGCTGACCAGGTGACGATGGAAGCGCGTAACTACGGGCTGGCGCGTCATTACGATCCA
TTTATCGTCAACACCGTGGTCGGCTTTATTGGGCCGGAGTATCTCTACAACGACCGCCAGATTATCCGTGCGGGCTTAGA
AGATCACTTTATGGGCAAACTGAGCGGCATCTCTATGGGCTGTGACTGCTGCTACACCAACCACGCTGACGCTGACCAGA
ACCTCAACGAAAACCTGATGATCCTGCTCGCCACCGCAGGCTGTAACTACATCATGGGGATGCCGCTGGGTGATGACATC
ATGCTCAACTACCAGACTACGGCCTTCCACGACACCGCCACTGTGCGTCAGTTACTCAACCTGCGCCCGTCACCGGAGTT
TGAACGCTGGCTGGAAAGCATGGGCATTATGGCAAACGGTCGCCTGACCAAACGGGCGGGCGATCCGTCACTGTTCTTCT
GA

Upstream 100 bases:

>100_bases
GCGCGGGGGACTATATCGACATTGGTACGCCTCTTTTTGGCGGATCGGTTGTGCCGGTGACGGTGAAATCACTCGCATTT
CCTTCCTGAGGGAACGACTT

Downstream 100 bases:

>100_bases
TGACGCGGAGATAACACATCATGGATCAAAAACAGATTGAAGAAATTGTACGCAGCGTGATGGCGTCAATGGGACAAGCG
GCCCCCGCGCCGTCAGAAGC

Product: ethanolamine ammonia-lyase, large subunit, heavy chain

Products: NA

Alternate protein names: Ethanolamine ammonia-lyase large subunit

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQERVAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDV
NETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDP
FIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDI
MLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPSLFF

Sequences:

>Translated_453_residues
MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQERVAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDV
NETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDP
FIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDI
MLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPSLFF
>Mature_453_residues
MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQERVAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDV
NETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDP
FIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDI
MLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPSLFF

Specific function: Ethanolamine utilization. [C]

COG id: COG4303

COG function: function code E; Ethanolamine ammonia-lyase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082106, Length=453, Percent_Identity=100, Blast_Score=937, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EUTB_ECO57 (P0AEJ7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85887
- PIR:   H91042
- RefSeq:   NP_289002.1
- RefSeq:   NP_311339.1
- ProteinModelPortal:   P0AEJ7
- SMR:   P0AEJ7
- EnsemblBacteria:   EBESCT00000024872
- EnsemblBacteria:   EBESCT00000056694
- GeneID:   915295
- GeneID:   957672
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3706
- KEGG:   ecs:ECs3312
- GeneTree:   EBGT00050000009179
- HOGENOM:   HBG289204
- OMA:   IPYEEDC
- ProtClustDB:   PRK15067
- BioCyc:   ECOL83334:ECS3312-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR010628
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF018788

Pfam domain/function: PF06751 EutB

EC number: =4.3.1.7

Molecular weight: Translated: 49404; Mature: 49404

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQERVAAKQVLSEMTVADIR
CCEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHC
NNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSE
CCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHCCCHHH
VVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSARLQPNDTRDDVQSIAAQIYEG
HHHHHHHHHCCCCEEECCCCCCEEEECCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHH
LSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
HCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCC
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANF
CCHHHHHHHCCCCCHHHHCCEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCC
GADQVTMEARNYGLARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMG
CCHHEEHHHHHCCCHHCCCCHHHHHHHHHHCHHHHHCCHHHHHHCHHHHHHHHHCCCCCC
CDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDIMLNYQTTAFHDTATVRQLLN
CCEEECCCCCCCCCCCCCEEEEEEECCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHC
LRPSPEFERWLESMGIMANGRLTKRAGDPSLFF
CCCCCHHHHHHHHCCCEECCEEEECCCCCCCCC
>Mature Secondary Structure
MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQERVAAKQVLSEMTVADIR
CCEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHC
NNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSE
CCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHCCCHHH
VVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSARLQPNDTRDDVQSIAAQIYEG
HHHHHHHHHCCCCEEECCCCCCEEEECCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHH
LSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
HCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCC
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANF
CCHHHHHHHCCCCCHHHHCCEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCC
GADQVTMEARNYGLARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMG
CCHHEEHHHHHCCCHHCCCCHHHHHHHHHHCHHHHHCCHHHHHHCHHHHHHHHHCCCCCC
CDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDIMLNYQTTAFHDTATVRQLLN
CCEEECCCCCCCCCCCCCEEEEEEECCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHC
LRPSPEFERWLESMGIMANGRLTKRAGDPSLFF
CCCCCHHHHHHHHCCCEECCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796