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The map label for this gene is maeB [H]

Identifier: 218548104

GI number: 218548104

Start: 735754

End: 738033

Strand: Direct

Name: maeB [H]

Synonym: EFER_0716

Alternate gene names: 218548104

Gene position: 735754-738033 (Clockwise)

Preceding gene: 218548101

Following gene: 218548105

Centisome position: 16.03

GC content: 50.13

Gene sequence:

>2280_bases
ATGGATGACCAATTAAAACAAAGCGCTCTTGATTTTCATGAATTTCCAGTACCAGGGAAAATCCAGGTTTCTCCTACCAA
ACCTCTTGCAACGCAGCGCGATCTGGCGCTGGCTTACTCACCTGGCGTTGCCGCTCCTTGCCTGGAAATCGAAAAAGATC
CACTGGCCTCTTACAAATACACCGCCCGTGGCAATCTGGTCGCAGTAATTTCTAACGGTACGGCAGTACTGGGACTGGGC
AATATTGGTGCGCTGGCAGGCAAACCAGTGATGGAAGGTAAGGGCGTTCTGTTTAAGAAATTCGCAGGTATTGATGTCTT
TGATATTGAAGTTGATGAGCTAAACCCGGACAAATTTATTGATGTTGTGGCAGCTCTGGAGCCAACCTTCGGTGGTATCA
ACCTTGAGGATATCAAGGCGCCTGAGTGCTTCTATATTGAGCAGAAGCTGCGTGAGCGCATGAATATTCCGGTGTTCCAC
GATGACCAGCATGGTACGGCCATTATCAGCACAGCGGCAATTATCAACGGATTGCGCGTAGTCGAGAAAAACATCTCTGA
TGTGCGAATGGTAGTTTCCGGTGCGGGCGCAGCAGCTATCGCCTGTATGAACCTGATGGTGGCACTGGGGATGCAGAAAC
ACAATATTGTGGTCTGTGACTCGAAAGGCGTTATTTATCAGGGACGTGAACCCAACATGGCAGAAACCAAAGCGGCTTAC
GCGGTGGTTGATGATGGAAAACGCACTCTCGATGATGTGATTGAAGGGGCTGATATTTTCCTCGGTTGCTCTGGCCCGAA
AGTGCTGACGCAAGAAATGGTCAAAAAAATGGCCCGTGCGCCAATGATCCTTGCTTTGGCAAACCCGGAACCAGAAATCC
TGCCACCACTGGCAAAAGAAGTTCGCCCGGATGCCATTATCTGTACTGGTCGTTCTGACTATCCGAACCAGGTTAACAAT
GTGCTCTGCTTCCCGTTCATCTTCCGTGGAGCGCTGGATGTAGGCGCAACAGCGATTAATGAAGAGATGAAACTGGCGGC
GGTACGCGCGATTGCCGAACTTGCTCACGCTGAACAGAGCGAAGTCGTTGCTTCTGCTTATGGTGATCAGGATCTGAGCT
TTGGTCCGGAATATATTATTCCGAAACCGTTCGATCCACGTCTGATCGTCAAGATCGCACCTGCGGTGGCAAAAGCTGCA
ATGGATTCTGGAGTAGCGACGCGGCCGATTGCGGATTTCGACGTTTATGTCGATAAGCTGACCGAGTTTGTCTACAAAAC
CAACTTATTCATGAAGCCTATTTTCTCCCAGGCCCGTAAAGCGCCGAAACGTGTTGTATTACCGGAAGGGGAAGAAGTTC
GTATCCTGCACGCCACTCAGGAACTGATCACATTGGGTCTGGCAAAACCGATCCTGATTGGTCGCCCAAATGTGATCGAA
ATGCGGATCAAGAAACTGGGGCTTCATATTCAGGCGGGCGTCGATTTTGAGATCGTCAATAACGAATCCGATCCGCGCTT
TAAAGAGTACTGGAGTGAGTACTACAAGATCATGCAGCGTCGTGGCGTTTCGCAGGAACAGGCACAGCGAGCGGTGATCA
GTAACCCAACAGTAATTGGCGCAATCATGGTGCATCGTGGTGAAGCAGATGCAATGATTTGCGGCACCATTGGTGACTAT
AACGAGCATTATGCAATCCTGAAAAATGTCTTTGGTTACCGTGAAGGAGCGCATACCGCCGGGGCGATGAATGCACTTTT
ACTGCCCAGTGGTAACACCTTTATTGCAGATACTTATGTCAACGATGAACCTGATTCCGAAGAATTGGCAGAAATCACCC
TGATGGCGGCTGAAACGGTACGCCGTTTTGGTATTGAGCCACGAGTGGCGCTGCTGTCACATTCCAACTTTGGTTCCTCT
AATTGCCCGTCATCCAGCAAAATGCGTAAAGCGCTGGAACTTATTAAAGCGCGAGCTCCTGAACTGATGATTGATGGCGA
AATGCACGGTGATGCGGCGCTGGTAGAGGCTATTCGTAGCGACCGGATGCCGGACAGTCCACTGAAAGGTGCTGCCAATA
TTTTGGTTATGCCGAACATGGAAGCGGCGCGTATTAGTTACAACTTGCTACGCGTTTCCAGTTCGGAAGGTGTCACCGTT
GGCCCGGTATTAATGGGAATAGCGAAACCTGTTCATGTATTAACGCCGATCGCTTCCGTACGTCGTATCGTGAACATGGT
GGCCCTGGCGGTGGTTGAAGCGCAGACTCAGCCGCTGTAA

Upstream 100 bases:

>100_bases
GGACTTGCTCAAAGTCTGTGACCTGTAGGAAGGTAATAATGTGCGGCGCGTTAGCGGTAGCTGTACAACTTAATCGAACG
ATACGTGAAAGGAATACCCA

Downstream 100 bases:

>100_bases
TTCTTGTTTAATTATCACGCTTACCCTGAATATTCAGGGTAAGCGATGTTACTGCAATATATTAGTAAAGATTATTACCT
GTTTTTAGAATAATAAAAAA

Product: malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 759; Mature: 759

Protein sequence:

>759_residues
MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLASYKYTARGNLVAVISNGTAVLGLG
NIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELNPDKFIDVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
DDQHGTAIISTAAIINGLRVVEKNISDVRMVVSGAGAAAIACMNLMVALGMQKHNIVVCDSKGVIYQGREPNMAETKAAY
AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNN
VLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAA
MDSGVATRPIADFDVYVDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEVRILHATQELITLGLAKPILIGRPNVIE
MRIKKLGLHIQAGVDFEIVNNESDPRFKEYWSEYYKIMQRRGVSQEQAQRAVISNPTVIGAIMVHRGEADAMICGTIGDY
NEHYAILKNVFGYREGAHTAGAMNALLLPSGNTFIADTYVNDEPDSEELAEITLMAAETVRRFGIEPRVALLSHSNFGSS
NCPSSSKMRKALELIKARAPELMIDGEMHGDAALVEAIRSDRMPDSPLKGAANILVMPNMEAARISYNLLRVSSSEGVTV
GPVLMGIAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL

Sequences:

>Translated_759_residues
MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLASYKYTARGNLVAVISNGTAVLGLG
NIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELNPDKFIDVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
DDQHGTAIISTAAIINGLRVVEKNISDVRMVVSGAGAAAIACMNLMVALGMQKHNIVVCDSKGVIYQGREPNMAETKAAY
AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNN
VLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAA
MDSGVATRPIADFDVYVDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEVRILHATQELITLGLAKPILIGRPNVIE
MRIKKLGLHIQAGVDFEIVNNESDPRFKEYWSEYYKIMQRRGVSQEQAQRAVISNPTVIGAIMVHRGEADAMICGTIGDY
NEHYAILKNVFGYREGAHTAGAMNALLLPSGNTFIADTYVNDEPDSEELAEITLMAAETVRRFGIEPRVALLSHSNFGSS
NCPSSSKMRKALELIKARAPELMIDGEMHGDAALVEAIRSDRMPDSPLKGAANILVMPNMEAARISYNLLRVSSSEGVTV
GPVLMGIAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL
>Mature_759_residues
MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLASYKYTARGNLVAVISNGTAVLGLG
NIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELNPDKFIDVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
DDQHGTAIISTAAIINGLRVVEKNISDVRMVVSGAGAAAIACMNLMVALGMQKHNIVVCDSKGVIYQGREPNMAETKAAY
AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNN
VLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAA
MDSGVATRPIADFDVYVDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEVRILHATQELITLGLAKPILIGRPNVIE
MRIKKLGLHIQAGVDFEIVNNESDPRFKEYWSEYYKIMQRRGVSQEQAQRAVISNPTVIGAIMVHRGEADAMICGTIGDY
NEHYAILKNVFGYREGAHTAGAMNALLLPSGNTFIADTYVNDEPDSEELAEITLMAAETVRRFGIEPRVALLSHSNFGSS
NCPSSSKMRKALELIKARAPELMIDGEMHGDAALVEAIRSDRMPDSPLKGAANILVMPNMEAARISYNLLRVSSSEGVTV
GPVLMGIAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=402, Percent_Identity=25.3731343283582, Blast_Score=79, Evalue=2e-14,
Organism=Escherichia coli, GI1788806, Length=759, Percent_Identity=94.729907773386, Blast_Score=1493, Evalue=0.0,
Organism=Escherichia coli, GI1788800, Length=326, Percent_Identity=32.8220858895706, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI1788635, Length=349, Percent_Identity=29.512893982808, Blast_Score=158, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17537199, Length=398, Percent_Identity=25.6281407035176, Blast_Score=66, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322823, Length=268, Percent_Identity=30.5970149253731, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24646388, Length=346, Percent_Identity=27.4566473988439, Blast_Score=81, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24646386, Length=346, Percent_Identity=27.4566473988439, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21356279, Length=474, Percent_Identity=24.4725738396624, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281362672, Length=474, Percent_Identity=24.4725738396624, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281362674, Length=474, Percent_Identity=24.4725738396624, Blast_Score=77, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 82347; Mature: 82347

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLASYKY
CCCHHHHHHCCHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEE
TARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELNPDKFI
ECCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEEHCCCCEEEEEEECCCCHHHHH
DVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFHDDQHGTAIISTAAIINGLRV
HHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEHHHHHHHHHHH
VEKNISDVRMVVSGAGAAAIACMNLMVALGMQKHNIVVCDSKGVIYQGREPNMAETKAAY
HHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHEEE
AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKE
EEECCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHH
VRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQS
CCCCEEEEECCCCCCHHHCCEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCHHH
EVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAAMDSGVATRPIADFDVYVDKL
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHH
TEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEVRILHATQELITLGLAKPILIGRPNVIE
HHHHHHHCHHHHHHHHHHHCCCCEEECCCCCCEEEEEHHHHHHHHHCCCCEEECCCCHHH
MRIKKLGLHIQAGVDFEIVNNESDPRFKEYWSEYYKIMQRRGVSQEQAQRAVISNPTVIG
HHHHHHCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEE
AIMVHRGEADAMICGTIGDYNEHYAILKNVFGYREGAHTAGAMNALLLPSGNTFIADTYV
EEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEEEC
NDEPDSEELAEITLMAAETVRRFGIEPRVALLSHSNFGSSNCPSSSKMRKALELIKARAP
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
ELMIDGEMHGDAALVEAIRSDRMPDSPLKGAANILVMPNMEAARISYNLLRVSSSEGVTV
CEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHEEEEEEEEECCCCCEE
GPVLMGIAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLASYKY
CCCHHHHHHCCHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEE
TARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELNPDKFI
ECCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEEHCCCCEEEEEEECCCCHHHHH
DVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFHDDQHGTAIISTAAIINGLRV
HHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEHHHHHHHHHHH
VEKNISDVRMVVSGAGAAAIACMNLMVALGMQKHNIVVCDSKGVIYQGREPNMAETKAAY
HHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHEEE
AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKE
EEECCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHH
VRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQS
CCCCEEEEECCCCCCHHHCCEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCHHH
EVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAAMDSGVATRPIADFDVYVDKL
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHH
TEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEVRILHATQELITLGLAKPILIGRPNVIE
HHHHHHHCHHHHHHHHHHHCCCCEEECCCCCCEEEEEHHHHHHHHHCCCCEEECCCCHHH
MRIKKLGLHIQAGVDFEIVNNESDPRFKEYWSEYYKIMQRRGVSQEQAQRAVISNPTVIG
HHHHHHCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEE
AIMVHRGEADAMICGTIGDYNEHYAILKNVFGYREGAHTAGAMNALLLPSGNTFIADTYV
EEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEEEC
NDEPDSEELAEITLMAAETVRRFGIEPRVALLSHSNFGSSNCPSSSKMRKALELIKARAP
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
ELMIDGEMHGDAALVEAIRSDRMPDSPLKGAANILVMPNMEAARISYNLLRVSSSEGVTV
CEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHEEEEEEEEECCCCCEE
GPVLMGIAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]