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The map label for this gene is uraA [H]

Identifier: 218548070

GI number: 218548070

Start: 695027

End: 696316

Strand: Direct

Name: uraA [H]

Synonym: EFER_0679

Alternate gene names: 218548070

Gene position: 695027-696316 (Clockwise)

Preceding gene: 218548069

Following gene: 218548071

Centisome position: 15.15

GC content: 50.39

Gene sequence:

>1290_bases
ATGACGCGCCGTGCTATCGGGGTGAGTGAAAGACCGCCGCTTTTACAGACAATCCCGCTTAGTTTGCAACACCTGTTCGC
CATGTTTGGTGCAACTGTACTGGTGCCCGTTCTGTTTAATATTAATCCGGCAACCGTTCTGTTATTCAACGGCATCGGAA
CCTTGCTGTATCTCTTTATCTGTAAAGGAAAAATCCCCGCCTATCTCGGGTCCAGTTTCGCTTTTATCTCCCCGGTTTTG
CTGTTGCTGCCGTTAGGTTATGAAGTGGCACTGGGTGGTTTTATCATGTGTGGTGTACTGTTTTGCCTGGTGTCTTTTAT
TGTGAAGAAAGCAGGGACTGGTTGGCTGGATGTTATGTTCCCACCAGCTGCCATGGGCGCAATCGTTGCCGTCATTGGTC
TTGAACTGGCGGGCGTTGCAGCGAACATGGCCGGGTTACTGCCAGCAGAAGGTCAGGCACCTGATGGTAAAACGATTTTT
GTTTCCCTGGTGACGCTGTCAGTCACGGTACTGGGGTCCGTATTGTTTCGTGGTTTCCTGGCGATAATCCCGATTCTTAT
CGGTGTGCTGGTGGGTTATGCCCTTTCGTTTGTGATGGGAATGGTTGATACCACACCAATTGTGAACGCCCACTGGTTTG
CATTACCGACTATCTATACGCCGCGTTTTGAGTGGTTTGCTATTCTTACGATTCTGCCAGCAGCGTTGGTGGTGATTGCG
GAACATGTCGGTCATCTGGTGGTAACGGCGAATATCGTGAAAAAAGATCTGATCCGTGATCCGGGTCTGCACCGTTCGAT
GTTTGCTAATGGCCTGTCGACCGTGATTTCTGGTTTCTTTGGTTCTACGCCGAATACCACCTACGGTGAAAACATTGGTG
TCATGGCCATTACTCGCGTCTACAGTACCTGGGTCATCGGCGGTGCGGCGATTATCGCTATTCTGCTTTCTTGTGTCGGG
AAACTGGCCGCTGCCATTCAGATGATCCCACTGCCAGTTATGGGCGGTGTATCGCTGCTGTTATACGGAGTCATTGGGGC
GTCGGGTATTCGTGTCCTGATTGAATCAAAAGTGGATTACAACAAAGCACAGAACCTGATCCTGACTTCGGTTATTTTGA
TTATCGGTGTCAGTGGCGCGAAGGTGCATATCGGTGCCGCAGAGCTGAAAGGTATGGCCCTGGCTACGATCGTAGGGATC
GGCCTGAGTCTGATCTTTAAACTGATTGGTGTCATGCGCCCGGAAGAGGTTATTCTGGAAGCAGAAGACGCAGATACACC
GCAGAAGTAA

Upstream 100 bases:

>100_bases
TTTGGTACGAAATAAATAATAAATTCTTTCTTAGCCGACTTTAAGAGTCGGCTTTTTTTTGACTAAAACGCTAATAAACT
TAATCGTAGAGGAAAACACT

Downstream 100 bases:

>100_bases
AGTAGCAACCGGGCAGTTCCGCTGCCCGGTTCTAACATCATGTAATTCTGTGGTAAACTTCTCACGATTTTGTGAAATCC
CTGGTTGAGGTATCTCTGAA

Product: uracil transporter

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFNINPATVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVL
LLLPLGYEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVMFPPAAMGAIVAVIGLELAGVAANMAGLLPAEGQAPDGKTIF
VSLVTLSVTVLGSVLFRGFLAIIPILIGVLVGYALSFVMGMVDTTPIVNAHWFALPTIYTPRFEWFAILTILPAALVVIA
EHVGHLVVTANIVKKDLIRDPGLHRSMFANGLSTVISGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIIAILLSCVG
KLAAAIQMIPLPVMGGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVHIGAAELKGMALATIVGI
GLSLIFKLIGVMRPEEVILEAEDADTPQK

Sequences:

>Translated_429_residues
MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFNINPATVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVL
LLLPLGYEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVMFPPAAMGAIVAVIGLELAGVAANMAGLLPAEGQAPDGKTIF
VSLVTLSVTVLGSVLFRGFLAIIPILIGVLVGYALSFVMGMVDTTPIVNAHWFALPTIYTPRFEWFAILTILPAALVVIA
EHVGHLVVTANIVKKDLIRDPGLHRSMFANGLSTVISGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIIAILLSCVG
KLAAAIQMIPLPVMGGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVHIGAAELKGMALATIVGI
GLSLIFKLIGVMRPEEVILEAEDADTPQK
>Mature_428_residues
TRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFNINPATVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLL
LLPLGYEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVMFPPAAMGAIVAVIGLELAGVAANMAGLLPAEGQAPDGKTIFV
SLVTLSVTVLGSVLFRGFLAIIPILIGVLVGYALSFVMGMVDTTPIVNAHWFALPTIYTPRFEWFAILTILPAALVVIAE
HVGHLVVTANIVKKDLIRDPGLHRSMFANGLSTVISGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIIAILLSCVGK
LAAAIQMIPLPVMGGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVHIGAAELKGMALATIVGIG
LSLIFKLIGVMRPEEVILEAEDADTPQK

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680148, Length=220, Percent_Identity=26.8181818181818, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI40316845, Length=220, Percent_Identity=26.8181818181818, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI44680145, Length=233, Percent_Identity=25.7510729613734, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI44680143, Length=233, Percent_Identity=25.7510729613734, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788843, Length=429, Percent_Identity=94.4055944055944, Blast_Score=799, Evalue=0.0,
Organism=Escherichia coli, GI87081818, Length=428, Percent_Identity=37.6168224299065, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI87082181, Length=448, Percent_Identity=27.0089285714286, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1790087, Length=442, Percent_Identity=26.2443438914027, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87082178, Length=370, Percent_Identity=25.9459459459459, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17541904, Length=233, Percent_Identity=25.7510729613734, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17542262, Length=216, Percent_Identity=25, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21356175, Length=214, Percent_Identity=28.5046728971963, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 45121; Mature: 44990

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFNINPATVLLFNGIGTLLYLFI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHH
CKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVMF
HCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PPAAMGAIVAVIGLELAGVAANMAGLLPAEGQAPDGKTIFVSLVTLSVTVLGSVLFRGFL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
AIIPILIGVLVGYALSFVMGMVDTTPIVNAHWFALPTIYTPRFEWFAILTILPAALVVIA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCEECCCHHHHHHHHHHHHHHHHHH
EHVGHLVVTANIVKKDLIRDPGLHRSMFANGLSTVISGFFGSTPNTTYGENIGVMAITRV
HHHCHHEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
YSTWVIGGAAIIAILLSCVGKLAAAIQMIPLPVMGGVSLLLYGVIGASGIRVLIESKVDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCH
NKAQNLILTSVILIIGVSGAKVHIGAAELKGMALATIVGIGLSLIFKLIGVMRPEEVILE
HHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEE
AEDADTPQK
CCCCCCCCC
>Mature Secondary Structure 
TRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFNINPATVLLFNGIGTLLYLFI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHH
CKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVMF
HCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PPAAMGAIVAVIGLELAGVAANMAGLLPAEGQAPDGKTIFVSLVTLSVTVLGSVLFRGFL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
AIIPILIGVLVGYALSFVMGMVDTTPIVNAHWFALPTIYTPRFEWFAILTILPAALVVIA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCEECCCHHHHHHHHHHHHHHHHHH
EHVGHLVVTANIVKKDLIRDPGLHRSMFANGLSTVISGFFGSTPNTTYGENIGVMAITRV
HHHCHHEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
YSTWVIGGAAIIAILLSCVGKLAAAIQMIPLPVMGGVSLLLYGVIGASGIRVLIESKVDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCH
NKAQNLILTSVILIIGVSGAKVHIGAAELKGMALATIVGIGLSLIFKLIGVMRPEEVILE
HHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEE
AEDADTPQK
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]