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The map label for this gene is yfgF [H]

Identifier: 218548064

GI number: 218548064

Start: 686282

End: 688531

Strand: Direct

Name: yfgF [H]

Synonym: EFER_0673

Alternate gene names: 218548064

Gene position: 686282-688531 (Clockwise)

Preceding gene: 218548063

Following gene: 218548069

Centisome position: 14.96

GC content: 43.47

Gene sequence:

>2250_bases
ATGAAACTGAATGCCAAATATATAAAATTCCGAGATAAATGGTGGGCACTTCCGCTTATTCTTCCGTGTTTTTTACTTCC
TTTATTAGCTAGCGCAAATAGTTACACTCATATTAGTTCTGGTAACGTAACACTCTTTTACTTGCCTTTGGCATTAATGA
TTAGCCTGATGTTATTTTTTGGTTGGGGTGCATTGCCAGGCATCATATTATCGATATTTTTGCGTAAATATCATGATGTT
GGCTTATATGAAACACTGACTATATCAGCCAATTTTGTGCTCATTATTGTGCTAAGTTGGGGCGGATATCGGGTGTTTGT
TCCCCGCCGGAGTCACATTTCACATGGCAATGCGCAACTTCTCTTTCAGCGGCTTTTCTGGCAGGTTTTTTGCCCGGCAG
TCTTGTTTTTGTTGCTTTTCCAGTTTGCTTCTTTTGTCGGATTGTATGGCAGTCGCTCCAGCCAGATTGGCATAATGCCT
TTTAATATTAGTACATTAATAAATTTTCAGTCACTGCTGGTGGGCAATCTGGTCGGTGTTCCACTTTGCTACGTTATTAT
TCGTATTTTACGTAACCCATTGCATATATTTGGCTATTATTCTCAGCTAAAACAACAGGTTGACAGAAAGGTCAGCAAAA
AGGAATTTGTCTTGTGGTTAATGGTATTGACTGCATTGATGACTTTGCTCTGTATGCCATTGAATACCCGCAGTTCAATT
TTCAGCACCAATTACACCTTATCGCTGCTTCTACCGGTGATGTTGTGGGGAGCGATGCGTTATGGCTACCGATTGATCTC
ACTGATATGGGCGGCGGTTCTGATTATCGGTATTCATAATCACAAAAGTTATATGCCGTGGTATGCAGGATACGATGTTC
AGTTGGCGATTACTTCTTCGAGCTATCTGGTATTTTCTTTTATTATTAATTACATGGCAGTACTTGCTACCCGGCAGCGG
GCGGTGGTAAACCGCATTCGCCGCCTGGCTTATTTCGATCCTGTAGTCCATTTGCCCAATATTCGTTCCCTTAATCGCAC
TTTGGCGAGTGCTCCCTGGTCGGTTATTTGTTACTTGCGCGTACCTGGCATGGAAATGTTAGTAAAAAACTACGGCATCA
TGTTGCGTATTCAGTACAAGCAGAATATTTCCCACTGGCTCGCACCGATGCTGGAGAAAGATGAATATGTTTACCAGTTG
TCGGGTAATGATCTCGCATTACGTCTGAATACAGAAGGCCACCAGGAGCGTATTGAAGCGATAGATAAGCACCTGAAAAA
TTTCCGCTTTATGTGGGACGGAATGCCACTCCAGCCACAAATCGGTATCAGTTATTGTTATGTACGTTCGCCAGTTAACC
ATATCTATTTATTACTTGGTGAATTAAGTACTGTCGCAGAGCTCTCTATCGCGACAAATGCGCCAGAAAGTATGCAACGT
CGCGGCGCGATGCATGTACAAAGAGACCTGAAAGATAAAGTAGCGATGATGAACCGTCTGCAACAGGCGCTTGAGCATGA
CCGTTTTTGCCTGATGGCGCAGCCGATTGTTGGTGTGCGGGGGGATGTTTATCACGAAATTTTGCTGCGTATGGAAGATG
ACGCTAACGGCAGGATCAATCCGGATATTTTCCTTCCAGTTGCCCACGAGTTCGGTTTGTCTTCCGCAATTGATCTATGG
GTGATTGAACATACTCTGCAGTTTATGGCCGTAAACCGGGAGAAGATCCCGGCGCAGCGCTATGCGATTAACTTATCTCC
AACATCTGTGTGTCGCCAGCGTTTACCGGAAGAGGTCAGGCATTTGTTGACCAAATACCAAATCGAAGCATGGCAGCTTA
TTTTCGAGGTTACCGAAAGTAATGCACTGACAAATATGGAACAGGCGCAAAACACCTTATTACAGTTGCAGGAACTAGGC
TGTCGAATAGCCATTGATGATTTCGGTACAGGTTATGCCAGTTACGCACGGCTGAAAAACGTGAATGCCGATATTATGAA
AATTGATGGTAGTTTTATCCGTAATATTGTCTCTAACAGCCTCGATTACCAGATTGTGGCATCGATCTGCCATCTTGCAC
GAATGAAAAAAATGCAGGTGGTTGCTGAATATGTAGAAAATGAAGAGATCCATCAGGCGGCATTGTCGCTGGGAATCGAT
TATTTTCAGGGTAATTTCTTTGGTGAGGCGAAACCGCTAAACGAAATTGTTGCGCAGAATGAGCAGCTAAAGAAATGCGT
CAATCAATGA

Upstream 100 bases:

>100_bases
GTCACCTCATTTGTGATGTTTATCATTACTATAATCTTAAGTCAGGTGGGATGCCTGTCTGTAAACACCCTCAGGGGAGA
GGGGTTTCTACTGAGCATCT

Downstream 100 bases:

>100_bases
ATCAAAACGCGTAATAAGGCTGGCCCGATGTGAAATCGGGCCACTTGTATCTCTTATGCTGTAACGCACATTATTTAGAT
ATACCTTCTTCTTCAATCTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 749; Mature: 749

Protein sequence:

>749_residues
MKLNAKYIKFRDKWWALPLILPCFLLPLLASANSYTHISSGNVTLFYLPLALMISLMLFFGWGALPGIILSIFLRKYHDV
GLYETLTISANFVLIIVLSWGGYRVFVPRRSHISHGNAQLLFQRLFWQVFCPAVLFLLLFQFASFVGLYGSRSSQIGIMP
FNISTLINFQSLLVGNLVGVPLCYVIIRILRNPLHIFGYYSQLKQQVDRKVSKKEFVLWLMVLTALMTLLCMPLNTRSSI
FSTNYTLSLLLPVMLWGAMRYGYRLISLIWAAVLIIGIHNHKSYMPWYAGYDVQLAITSSSYLVFSFIINYMAVLATRQR
AVVNRIRRLAYFDPVVHLPNIRSLNRTLASAPWSVICYLRVPGMEMLVKNYGIMLRIQYKQNISHWLAPMLEKDEYVYQL
SGNDLALRLNTEGHQERIEAIDKHLKNFRFMWDGMPLQPQIGISYCYVRSPVNHIYLLLGELSTVAELSIATNAPESMQR
RGAMHVQRDLKDKVAMMNRLQQALEHDRFCLMAQPIVGVRGDVYHEILLRMEDDANGRINPDIFLPVAHEFGLSSAIDLW
VIEHTLQFMAVNREKIPAQRYAINLSPTSVCRQRLPEEVRHLLTKYQIEAWQLIFEVTESNALTNMEQAQNTLLQLQELG
CRIAIDDFGTGYASYARLKNVNADIMKIDGSFIRNIVSNSLDYQIVASICHLARMKKMQVVAEYVENEEIHQAALSLGID
YFQGNFFGEAKPLNEIVAQNEQLKKCVNQ

Sequences:

>Translated_749_residues
MKLNAKYIKFRDKWWALPLILPCFLLPLLASANSYTHISSGNVTLFYLPLALMISLMLFFGWGALPGIILSIFLRKYHDV
GLYETLTISANFVLIIVLSWGGYRVFVPRRSHISHGNAQLLFQRLFWQVFCPAVLFLLLFQFASFVGLYGSRSSQIGIMP
FNISTLINFQSLLVGNLVGVPLCYVIIRILRNPLHIFGYYSQLKQQVDRKVSKKEFVLWLMVLTALMTLLCMPLNTRSSI
FSTNYTLSLLLPVMLWGAMRYGYRLISLIWAAVLIIGIHNHKSYMPWYAGYDVQLAITSSSYLVFSFIINYMAVLATRQR
AVVNRIRRLAYFDPVVHLPNIRSLNRTLASAPWSVICYLRVPGMEMLVKNYGIMLRIQYKQNISHWLAPMLEKDEYVYQL
SGNDLALRLNTEGHQERIEAIDKHLKNFRFMWDGMPLQPQIGISYCYVRSPVNHIYLLLGELSTVAELSIATNAPESMQR
RGAMHVQRDLKDKVAMMNRLQQALEHDRFCLMAQPIVGVRGDVYHEILLRMEDDANGRINPDIFLPVAHEFGLSSAIDLW
VIEHTLQFMAVNREKIPAQRYAINLSPTSVCRQRLPEEVRHLLTKYQIEAWQLIFEVTESNALTNMEQAQNTLLQLQELG
CRIAIDDFGTGYASYARLKNVNADIMKIDGSFIRNIVSNSLDYQIVASICHLARMKKMQVVAEYVENEEIHQAALSLGID
YFQGNFFGEAKPLNEIVAQNEQLKKCVNQ
>Mature_749_residues
MKLNAKYIKFRDKWWALPLILPCFLLPLLASANSYTHISSGNVTLFYLPLALMISLMLFFGWGALPGIILSIFLRKYHDV
GLYETLTISANFVLIIVLSWGGYRVFVPRRSHISHGNAQLLFQRLFWQVFCPAVLFLLLFQFASFVGLYGSRSSQIGIMP
FNISTLINFQSLLVGNLVGVPLCYVIIRILRNPLHIFGYYSQLKQQVDRKVSKKEFVLWLMVLTALMTLLCMPLNTRSSI
FSTNYTLSLLLPVMLWGAMRYGYRLISLIWAAVLIIGIHNHKSYMPWYAGYDVQLAITSSSYLVFSFIINYMAVLATRQR
AVVNRIRRLAYFDPVVHLPNIRSLNRTLASAPWSVICYLRVPGMEMLVKNYGIMLRIQYKQNISHWLAPMLEKDEYVYQL
SGNDLALRLNTEGHQERIEAIDKHLKNFRFMWDGMPLQPQIGISYCYVRSPVNHIYLLLGELSTVAELSIATNAPESMQR
RGAMHVQRDLKDKVAMMNRLQQALEHDRFCLMAQPIVGVRGDVYHEILLRMEDDANGRINPDIFLPVAHEFGLSSAIDLW
VIEHTLQFMAVNREKIPAQRYAINLSPTSVCRQRLPEEVRHLLTKYQIEAWQLIFEVTESNALTNMEQAQNTLLQLQELG
CRIAIDDFGTGYASYARLKNVNADIMKIDGSFIRNIVSNSLDYQIVASICHLARMKKMQVVAEYVENEEIHQAALSLGID
YFQGNFFGEAKPLNEIVAQNEQLKKCVNQ

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=745, Percent_Identity=74.6308724832215, Blast_Score=1173, Evalue=0.0,
Organism=Escherichia coli, GI87082096, Length=710, Percent_Identity=29.4366197183099, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI87081921, Length=462, Percent_Identity=27.2727272727273, Blast_Score=129, Evalue=8e-31,
Organism=Escherichia coli, GI1790496, Length=248, Percent_Identity=32.6612903225806, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87081743, Length=264, Percent_Identity=29.9242424242424, Blast_Score=126, Evalue=6e-30,
Organism=Escherichia coli, GI1787541, Length=437, Percent_Identity=25.858123569794, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI226510982, Length=348, Percent_Identity=27.5862068965517, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1786507, Length=240, Percent_Identity=28.75, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87081980, Length=239, Percent_Identity=29.2887029288703, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1788502, Length=246, Percent_Identity=26.4227642276423, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87081845, Length=230, Percent_Identity=27.8260869565217, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787055, Length=251, Percent_Identity=26.2948207171315, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1789650, Length=244, Percent_Identity=23.7704918032787, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1787410, Length=153, Percent_Identity=30.0653594771242, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 85986; Mature: 85986

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNAKYIKFRDKWWALPLILPCFLLPLLASANSYTHISSGNVTLFYLPLALMISLMLFF
CCCCCHHEEECCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHHHHHHHHHH
GWGALPGIILSIFLRKYHDVGLYETLTISANFVLIIVLSWGGYRVFVPRRSHISHGNAQL
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEEECCCEEEEEECCCCCCCCHHHH
LFQRLFWQVFCPAVLFLLLFQFASFVGLYGSRSSQIGIMPFNISTLINFQSLLVGNLVGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHH
PLCYVIIRILRNPLHIFGYYSQLKQQVDRKVSKKEFVLWLMVLTALMTLLCMPLNTRSSI
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
FSTNYTLSLLLPVMLWGAMRYGYRLISLIWAAVLIIGIHNHKSYMPWYAGYDVQLAITSS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEEECC
SYLVFSFIINYMAVLATRQRAVVNRIRRLAYFDPVVHLPNIRSLNRTLASAPWSVICYLR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHCCCCEEEEEEE
VPGMEMLVKNYGIMLRIQYKQNISHWLAPMLEKDEYVYQLSGNDLALRLNTEGHQERIEA
CCCHHHHHHCCCEEEEEEEHHCHHHHHHHHHCCCCEEEEECCCEEEEEECCCCHHHHHHH
IDKHLKNFRFMWDGMPLQPQIGISYCYVRSPVNHIYLLLGELSTVAELSIATNAPESMQR
HHHHHHHCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RGAMHVQRDLKDKVAMMNRLQQALEHDRFCLMAQPIVGVRGDVYHEILLRMEDDANGRIN
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCC
PDIFLPVAHEFGLSSAIDLWVIEHTLQFMAVNREKIPAQRYAINLSPTSVCRQRLPEEVR
CCEEEEEHHHCCCCHHHHHHHHHHHHHHHHCCHHHCCHHHEEEECCHHHHHHHHCHHHHH
HLLTKYQIEAWQLIFEVTESNALTNMEQAQNTLLQLQELGCRIAIDDFGTGYASYARLKN
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHC
VNADIMKIDGSFIRNIVSNSLDYQIVASICHLARMKKMQVVAEYVENEEIHQAALSLGID
CCCCEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
YFQGNFFGEAKPLNEIVAQNEQLKKCVNQ
HHCCCCCCCCCCHHHHHCCCHHHHHHHCC
>Mature Secondary Structure
MKLNAKYIKFRDKWWALPLILPCFLLPLLASANSYTHISSGNVTLFYLPLALMISLMLFF
CCCCCHHEEECCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHHHHHHHHHH
GWGALPGIILSIFLRKYHDVGLYETLTISANFVLIIVLSWGGYRVFVPRRSHISHGNAQL
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEEECCCEEEEEECCCCCCCCHHHH
LFQRLFWQVFCPAVLFLLLFQFASFVGLYGSRSSQIGIMPFNISTLINFQSLLVGNLVGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHH
PLCYVIIRILRNPLHIFGYYSQLKQQVDRKVSKKEFVLWLMVLTALMTLLCMPLNTRSSI
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
FSTNYTLSLLLPVMLWGAMRYGYRLISLIWAAVLIIGIHNHKSYMPWYAGYDVQLAITSS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEEECC
SYLVFSFIINYMAVLATRQRAVVNRIRRLAYFDPVVHLPNIRSLNRTLASAPWSVICYLR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHCCCCEEEEEEE
VPGMEMLVKNYGIMLRIQYKQNISHWLAPMLEKDEYVYQLSGNDLALRLNTEGHQERIEA
CCCHHHHHHCCCEEEEEEEHHCHHHHHHHHHCCCCEEEEECCCEEEEEECCCCHHHHHHH
IDKHLKNFRFMWDGMPLQPQIGISYCYVRSPVNHIYLLLGELSTVAELSIATNAPESMQR
HHHHHHHCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RGAMHVQRDLKDKVAMMNRLQQALEHDRFCLMAQPIVGVRGDVYHEILLRMEDDANGRIN
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCC
PDIFLPVAHEFGLSSAIDLWVIEHTLQFMAVNREKIPAQRYAINLSPTSVCRQRLPEEVR
CCEEEEEHHHCCCCHHHHHHHHHHHHHHHHCCHHHCCHHHEEEECCHHHHHHHHCHHHHH
HLLTKYQIEAWQLIFEVTESNALTNMEQAQNTLLQLQELGCRIAIDDFGTGYASYARLKN
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHC
VNADIMKIDGSFIRNIVSNSLDYQIVASICHLARMKKMQVVAEYVENEEIHQAALSLGID
CCCCEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
YFQGNFFGEAKPLNEIVAQNEQLKKCVNQ
HHCCCCCCCCCCHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]