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The map label for this gene is yfhQ [H]

Identifier: 218548031

GI number: 218548031

Start: 639576

End: 640316

Strand: Direct

Name: yfhQ [H]

Synonym: EFER_0640

Alternate gene names: 218548031

Gene position: 639576-640316 (Clockwise)

Preceding gene: 218548027

Following gene: 218548032

Centisome position: 13.94

GC content: 53.58

Gene sequence:

>741_bases
ATGCTGCAAAATATTCGAATTGTGCTGGTGGAGACGTCACACACCGGCAATATGGGTTCTGTTGCCCGTGCCATGAAAAC
AATGGGCTTAACCAATCTGTGGCTGGTTAATCCACTAGTGAAACCTGACTCCCAGGCGATTGCTCTGGCAGCAGGGGCCA
GCGATGTGATTGGTAATGCTCACATTGTCGATACACTCGACGAAGCGTTAGCCGGTTGTAGCTTGGTGGTTGGCACCAGT
GCACGCTCGCGCACGCTGCCGTGGCCGATGCTCGACCCGCGCGAATGCGGTTTGAAAAGCGTCGCTGAAGCGGCAAATAC
GCCGGTGGCACTGGTTTTTGGTCGTGAGCGTGTCGGCTTGACCAATGAAGAGTTGCAAAAGTGCCATTATCATGTCGCCA
TTACCGCTAACCCGGAATACAGTTCGCTTAACCTGGCGATGGCTGTTCAGGTTATTGCCTATGAAGTGCGCATGGCCTGG
CTGGCGACTCAGGAGAACAGCGAGCAGGTTGAACATGACGAGACGCCGTATCCGCTGGTCGATGATCTGGAACGTTTTTA
CGGTCATCTTGAACAAACGCTGCTGGCAACCGGCTTTATCCGTGAAAACCATCCGGGGCAGGTGATGAATAAGCTGCGTC
GTCTGTTTACCCGTGCGCGCCCGGAAAGCCAGGAGTTGAATATCCTGCGCGGGATTCTGGCTTCTATTGAGCAGCAGAAT
AAAGGTAACAAGGCCGAATAA

Upstream 100 bases:

>100_bases
GTTAAAGAACGGGAAAACGGCGCAGAGTATAGCAGCGAGATCGGCATCTGTCTTCGTTTTATGATAAGATGCGTGAATAA
TCTTTAGATGAAGAAAGACA

Downstream 100 bases:

>100_bases
CCGAGGTTGCCTGATACGGCGCTTAACGCGTCTTGTCAGGCCTACATTGTACTGAACCCCTGGTCGGATAAAGTGTTTAC
GCCGCACCCGACAACAGGTA

Product: methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAITANPEYSSLNLAMAVQVIAYEVRMAW
LATQENSEQVEHDETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
KGNKAE

Sequences:

>Translated_246_residues
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAITANPEYSSLNLAMAVQVIAYEVRMAW
LATQENSEQVEHDETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
KGNKAE
>Mature_246_residues
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAITANPEYSSLNLAMAVQVIAYEVRMAW
LATQENSEQVEHDETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
KGNKAE

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=246, Percent_Identity=98.780487804878, Blast_Score=503, Evalue=1e-144,
Organism=Escherichia coli, GI1790865, Length=153, Percent_Identity=37.2549019607843, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27094; Mature: 27094

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNA
CCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCEEEEECCCHHHCCCC
HIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEEEEECCCCCCC
TNEELQKCHYHVAITANPEYSSLNLAMAVQVIAYEVRMAWLATQENSEQVEHDETPYPLV
CHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHH
DDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
KGNKAE
CCCCCC
>Mature Secondary Structure
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNA
CCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCEEEEECCCHHHCCCC
HIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEEEEECCCCCCC
TNEELQKCHYHVAITANPEYSSLNLAMAVQVIAYEVRMAWLATQENSEQVEHDETPYPLV
CHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHH
DDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
KGNKAE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA