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The map label for this gene is yfiF [H]

Identifier: 218547893

GI number: 218547893

Start: 494393

End: 495430

Strand: Direct

Name: yfiF [H]

Synonym: EFER_0493

Alternate gene names: 218547893

Gene position: 494393-495430 (Clockwise)

Preceding gene: 218547892

Following gene: 218547895

Centisome position: 10.77

GC content: 56.26

Gene sequence:

>1038_bases
ATGAACGATGAAATGAAAGGTAAAAGCGGCAAGGTCAAAGTGATGTATGTCCGCAGTGATGATGATTCTGATAAACGTAC
CCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATG
ACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGAGTCGCCGTGGCGCACAGTTTCCCGCGCGCCGGGTGATGAGACG
CCGGAAAAGGCCGATCACGGTGGTATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGA
AACCCGCGTCTACGGCGAAAACGCCTGTCAGGCACTGTTCCAGAGCCGCCCCGAAGCGATTGTTCGCGCCTGGTTTATTC
AGAGCGTAACGCCACGTTTTAAAGAAGCATTGCGCTGGATGGCGGCAAACCGCAAAGCGTATCATGTGGTGGATGAAGCG
GAACTGACCAAAGCGTCAGGCACGGAACATCACGGCGGCGTTTGCTTCCTGATCAAAAAGCGTAACGGTACAACCGTGCA
GCAGTGGGTAAGTCAGGCAGGTGCGCAGGATTGTGTACTGGCACTGGAAAACGAATCTAACCCGCATAACCTGGGCGGCA
TGATGCGCAGTTGCGCACACTTCGGCGTGAAAGGCGTTGTGGTGCAGGATGCGGCGTTGCTGGAATCCGGTGCGGCTATT
CGTACCGCAGAAGGCGGTGCAGAGCATGTCCAGCCGATTACTGGCGACAACATTGTTAACGTGCTGGATGACTTCCGCCA
GGCGGGTTACACCGTAGTGACCACCTCCAGCGAGCAGGGTAAACCGCTGTTCAAAACCAGTCTGCCAGAGAAAATGGTGC
TGGTGCTGGGTCAGGAATATGAAGGGTTACCGGATGCCGCACGCGATCCGAACGATTTGCGCGTGAAGATTGACGGAACT
GGGAATGTTGCCGGGCTGAATATCTCTGTTGCAACCGGCGTTTTGCTTGGCGAATGGTGGCGTCAGAACAAAGCCTGA

Upstream 100 bases:

>100_bases
TCAATAGGTATGATAGTCGCAGGCTTTGGTACGAAATCATGGCGAACCGCGCGCACTTCGGGTAATCTGCGCGCTTCGCG
CAGCGCTGGTGGAGAAAAGC

Downstream 100 bases:

>100_bases
TAAATCAGCCGGGTGGAAATTCTGCCATCCGGCGTTTTCCCTCAAATTTACTCACTCTCTGCCGGTAATACTGGCATCCA
GTCAATCGGTTTCTCGCCAC

Product: methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDET
PEKADHGGISGKSFIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPEKMVLVLGQEYEGLPDAARDPNDLRVKIDGT
GNVAGLNISVATGVLLGEWWRQNKA

Sequences:

>Translated_345_residues
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDET
PEKADHGGISGKSFIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPEKMVLVLGQEYEGLPDAARDPNDLRVKIDGT
GNVAGLNISVATGVLLGEWWRQNKA
>Mature_345_residues
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDET
PEKADHGGISGKSFIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPEKMVLVLGQEYEGLPDAARDPNDLRVKIDGT
GNVAGLNISVATGVLLGEWWRQNKA

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=345, Percent_Identity=99.4202898550725, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI1790623, Length=247, Percent_Identity=27.9352226720648, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 37857; Mature: 37857

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPR
CCCCCCCCCCCEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRRQRAEETRVYGENACQALF
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEAELTKASGTEHHGGVCFLIKK
HCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCEEEEEEC
RNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
CCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHCCCEE
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPEKMVLVLGQEY
EECCCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCHHEEEEECCCC
EGLPDAARDPNDLRVKIDGTGNVAGLNISVATGVLLGEWWRQNKA
CCCCCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPR
CCCCCCCCCCCEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRRQRAEETRVYGENACQALF
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEAELTKASGTEHHGGVCFLIKK
HCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCEEEEEEC
RNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
CCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHCCCEE
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPEKMVLVLGQEY
EECCCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCHHEEEEECCCC
EGLPDAARDPNDLRVKIDGTGNVAGLNISVATGVLLGEWWRQNKA
CCCCCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]