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The map label for this gene is grpE [H]

Identifier: 218547868

GI number: 218547868

Start: 464174

End: 465226

Strand: Direct

Name: grpE [H]

Synonym: EFER_0459

Alternate gene names: 218547868

Gene position: 464174-465226 (Clockwise)

Preceding gene: 218547864

Following gene: 218547871

Centisome position: 10.12

GC content: 50.14

Gene sequence:

>1053_bases
TTGTTGCTGAGCGTTATCGGGGTCAAGGTCGGTCAGGAAACCCAGGTTGCCACGGTTGATTCCAATAACTTTAATATCGT
AACGGGCGAGTGTGCGCGCCGCGCCCAGCATATTACCGTCGCCACCAACGACTACCGCGAGATCAGCCAGTTGCCCAATC
TCCGCGAGCGTGCCAGTTTTCACATTCTTCAGTTGCAGTTCGTGAGCGATTTGTTGCTCAACGATGACCTCGTAACCTTT
TGTGCACAGCCAGCGGTAGAGCATTTCATGTGTTGTCAGTGCAGTAGGGTGCCGTGGGTGTCCCACAATGCCAATACACT
TGAAATGATTATTCATTTTTCCGAGGTCCTTGTTGCGAAGATTGATGACAATGTGAGTGCTTCCCTTGAAACCCTGAAAC
TGATCCCCATAATAAGCGAAGTTAGCGAGATGAATGCGAAAAAAAACGCGGAGAAATTCATGAGTAGTAAAGAACAGAAA
ACGCCTGAGGGGCAAGCCCCGGAAGAAATTATCATGGATCAGCACGAAGAGATTGAGGCAGTTGAGCCAGAAGCTTCTGC
TGAGCAGGTGGATCCGCGCGATGAAAAAATTGCGAATCTCGAAGCTCAGCTGGCTGAAGCCCAGATCCGTGAACGTGACG
GCATTTTGCGCGTAAAAGCCGAAATGGAAAACCTGCGTCGCCGTACTGAACTGGATATCGAAAAAGCACATAAATTCGCG
CTGGAGAAATTCATCAACGAATTGCTGCCGGTGATTGATAGCCTGGATCGTGCGCTGGAAGTGGCTGATAAAGCTAACCC
GGATATGTCCGCGATGGTTGAAGGTATTGAGCTGACGCTGAAGTCGATGCTGGATGTTGTGCGTAAGTTTGGCGTTGAAG
TGATCGCCGAAACTAACGTGCCGCTGGACCCGAACGTGCATCAGGCCATCGCAATGGTGGAATCTGATGACGTTGCGCCA
GGTAACGTACTGGGCATTATGCAGAAGGGTTATACGCTGAACGGTCGTACGATTCGTGCGGCGATGGTTACTGTAGCGAA
AGCAAAAGCTTAA

Upstream 100 bases:

>100_bases
TCGCGGTGCTGATGCGCTTTTGGCAATCTTGCTGACAGACTTGCGCTTCCAGCAAAAAACGTTTCTCGCTGATGTAGTGA
CCTTCCAGCACATCGGCTAA

Downstream 100 bases:

>100_bases
TTTCTGCTTTCGTAATAAATCACGGCCCAGCATGCGAATGCCGGGCCGTTTTCGTTACTCCGCCACACTCTCACGCAGCG
GTTTCACAGGAAAAACTTTT

Product: heat shock protein HSP70 cofactor

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MLLSVIGVKVGQETQVATVDSNNFNIVTGECARRAQHITVATNDYREISQLPNLRERASFHILQLQFVSDLLLNDDLVTF
CAQPAVEHFMCCQCSRVPWVSHNANTLEMIIHFSEVLVAKIDDNVSASLETLKLIPIISEVSEMNAKKNAEKFMSSKEQK
TPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKIANLEAQLAEAQIRERDGILRVKAEMENLRRRTELDIEKAHKFA
LEKFINELLPVIDSLDRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMVESDDVAP
GNVLGIMQKGYTLNGRTIRAAMVTVAKAKA

Sequences:

>Translated_350_residues
MLLSVIGVKVGQETQVATVDSNNFNIVTGECARRAQHITVATNDYREISQLPNLRERASFHILQLQFVSDLLLNDDLVTF
CAQPAVEHFMCCQCSRVPWVSHNANTLEMIIHFSEVLVAKIDDNVSASLETLKLIPIISEVSEMNAKKNAEKFMSSKEQK
TPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKIANLEAQLAEAQIRERDGILRVKAEMENLRRRTELDIEKAHKFA
LEKFINELLPVIDSLDRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMVESDDVAP
GNVLGIMQKGYTLNGRTIRAAMVTVAKAKA
>Mature_350_residues
MLLSVIGVKVGQETQVATVDSNNFNIVTGECARRAQHITVATNDYREISQLPNLRERASFHILQLQFVSDLLLNDDLVTF
CAQPAVEHFMCCQCSRVPWVSHNANTLEMIIHFSEVLVAKIDDNVSASLETLKLIPIISEVSEMNAKKNAEKFMSSKEQK
TPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKIANLEAQLAEAQIRERDGILRVKAEMENLRRRTELDIEKAHKFA
LEKFINELLPVIDSLDRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMVESDDVAP
GNVLGIMQKGYTLNGRTIRAAMVTVAKAKA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=169, Percent_Identity=30.1775147928994, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI40255109, Length=158, Percent_Identity=31.6455696202532, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788967, Length=197, Percent_Identity=98.9847715736041, Blast_Score=393, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17552458, Length=144, Percent_Identity=34.0277777777778, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324806, Length=181, Percent_Identity=32.5966850828729, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653432, Length=174, Percent_Identity=32.7586206896552, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 38937; Mature: 38937

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSVIGVKVGQETQVATVDSNNFNIVTGECARRAQHITVATNDYREISQLPNLRERASF
CEEEEEEEECCCCCEEEEEECCCCEEEECHHHHCCCEEEEECCCHHHHHHCCCHHHHHHH
HILQLQFVSDLLLNDDLVTFCAQPAVEHFMCCQCSRVPWVSHNANTLEMIIHFSEVLVAK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
IDDNVSASLETLKLIPIISEVSEMNAKKNAEKFMSSKEQKTPEGQAPEEIIMDQHEEIEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHCC
VEPEASAEQVDPRDEKIANLEAQLAEAQIRERDGILRVKAEMENLRRRTELDIEKAHKFA
CCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCHHHHHHHH
LEKFINELLPVIDSLDRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
PLDPNVHQAIAMVESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA
CCCCCHHHHHHHHCCCCCCCCHHHHHHHCCEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLLSVIGVKVGQETQVATVDSNNFNIVTGECARRAQHITVATNDYREISQLPNLRERASF
CEEEEEEEECCCCCEEEEEECCCCEEEECHHHHCCCEEEEECCCHHHHHHCCCHHHHHHH
HILQLQFVSDLLLNDDLVTFCAQPAVEHFMCCQCSRVPWVSHNANTLEMIIHFSEVLVAK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
IDDNVSASLETLKLIPIISEVSEMNAKKNAEKFMSSKEQKTPEGQAPEEIIMDQHEEIEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHCC
VEPEASAEQVDPRDEKIANLEAQLAEAQIRERDGILRVKAEMENLRRRTELDIEKAHKFA
CCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCHHHHHHHH
LEKFINELLPVIDSLDRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
PLDPNVHQAIAMVESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA
CCCCCHHHHHHHHCCCCCCCCHHHHHHHCCEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA