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The map label for this gene is int [H]

Identifier: 218547860

GI number: 218547860

Start: 458001

End: 459200

Strand: Reverse

Name: int [H]

Synonym: EFER_0450

Alternate gene names: 218547860

Gene position: 459200-458001 (Counterclockwise)

Preceding gene: 218547862

Following gene: 218547859

Centisome position: 10.01

GC content: 39.92

Gene sequence:

>1200_bases
GTGTCACGTACAACACGCCCTCTTACTAACACTGAACTATCACGGGCTAAAGCAACTGCAAAGGATCTAACGCTACATGA
TGGTAATGGACTTTTTCTACTCGTAAAAACGAACGGTAGGAAGCTGTGGCGTTTCCGATACCAGAAACCAGTAACAAAAC
AACGAACCATGATTGGTTTAGGGTCTTTCCCTGCCCTTTCTCTGGCTGAAGCCAGAAACACTCGTGCTGAGTATCTTTCC
TTGCTGGCCCGAGGTATTGATCCACAGATATACAGTGAGCAAGTCACTGTACAGCAACAGATTGCGGAAGATAGTATTTT
TTCAAACGTTGCCTCAAAATGGTTTGCCATAAAAAGAAACAACGTAACACCTGATTACGCAAAGGATGTATGGCGCTCCT
TAGAGAGAGATATTTTCCCTACTATCGGTGAAATTCCTGTTCAGAAAATAAAAGCTCGTACATTAATTGAAACCTTAGAA
CCAATTAAAGCTCGTGGAGCACTTGAAACTGTTCGTCGTTTGGTACAACGTATTAATGAAATTATGATTTATGCCGTTAA
TACTGGCTTAATTGACGCTAATCCTGCATCAGGTATTGGTATGGCATTTGAGAAACCTAAAAAGAAAAACATGCCAACAC
TACGACCTGAAGAATTACCGAAACTTATGTATTCTTTATCTATGTCAAATCTTTCTGTGCAGACCCGTTGTCTGATTGAA
TGGCAACTACTCACCCTTGTGCGCCCTTCAGAGGCTTCAGGCACTCAATGGAAGGAGATCGATTTTGATGCAAAACTCTG
GAGAATTCCTGCTGAAAGGATGAAAGCTAAGCGCGAACATATTGTACCTTTATCACCTAAGGCTTTAGATATTCTTGAAG
TTATGAAAACTATTAGTGCTCATCGTGAGTATGTTTTCCCAAGTAGAAATGATCCACAAAAACCCATGAATAGCCAAACT
GCAAATGCCGCTTTAAAAAGGATAGGATATGGTGGAAAACTCGTTGCTCATGGTTTACGTTCTATTGCCAGCACTGCAAT
GAATGAAGCCGAATTTAACGCTGATGTAATAGAATCAGCCTTAGCTCATAGCGATAAGAATGAAGTCAGAAAAGCTTATA
ACCGTTCTACTTACTTAGAACAAAGGAAGAATTTAATGAACTGGTGGAGTTCTTTTGTTCATAAATCTTTATCCTTTTAA

Upstream 100 bases:

>100_bases
GAATATCCCGCTAAATCCAGTGATTATTGGTATACGTTTAGGTATACGGTAAGATGTATACCTAAAAGCGTATACCAATT
CTCTCTGAGGTAGGATAATC

Downstream 100 bases:

>100_bases
TTTTTTCATTTGGAGAGTTTTCGATGGTTACAATGATACAAGCCGAAAATTTATTAAGAGAATTAGTAAACGAATATAGT
GACAGCGCCAAGATAAGAAA

Product: phage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSRTTRPLTNTELSRAKATAKDLTLHDGNGLFLLVKTNGRKLWRFRYQKPVTKQRTMIGLGSFPALSLAEARNTRAEYLS
LLARGIDPQIYSEQVTVQQQIAEDSIFSNVASKWFAIKRNNVTPDYAKDVWRSLERDIFPTIGEIPVQKIKARTLIETLE
PIKARGALETVRRLVQRINEIMIYAVNTGLIDANPASGIGMAFEKPKKKNMPTLRPEELPKLMYSLSMSNLSVQTRCLIE
WQLLTLVRPSEASGTQWKEIDFDAKLWRIPAERMKAKREHIVPLSPKALDILEVMKTISAHREYVFPSRNDPQKPMNSQT
ANAALKRIGYGGKLVAHGLRSIASTAMNEAEFNADVIESALAHSDKNEVRKAYNRSTYLEQRKNLMNWWSSFVHKSLSF

Sequences:

>Translated_399_residues
MSRTTRPLTNTELSRAKATAKDLTLHDGNGLFLLVKTNGRKLWRFRYQKPVTKQRTMIGLGSFPALSLAEARNTRAEYLS
LLARGIDPQIYSEQVTVQQQIAEDSIFSNVASKWFAIKRNNVTPDYAKDVWRSLERDIFPTIGEIPVQKIKARTLIETLE
PIKARGALETVRRLVQRINEIMIYAVNTGLIDANPASGIGMAFEKPKKKNMPTLRPEELPKLMYSLSMSNLSVQTRCLIE
WQLLTLVRPSEASGTQWKEIDFDAKLWRIPAERMKAKREHIVPLSPKALDILEVMKTISAHREYVFPSRNDPQKPMNSQT
ANAALKRIGYGGKLVAHGLRSIASTAMNEAEFNADVIESALAHSDKNEVRKAYNRSTYLEQRKNLMNWWSSFVHKSLSF
>Mature_398_residues
SRTTRPLTNTELSRAKATAKDLTLHDGNGLFLLVKTNGRKLWRFRYQKPVTKQRTMIGLGSFPALSLAEARNTRAEYLSL
LARGIDPQIYSEQVTVQQQIAEDSIFSNVASKWFAIKRNNVTPDYAKDVWRSLERDIFPTIGEIPVQKIKARTLIETLEP
IKARGALETVRRLVQRINEIMIYAVNTGLIDANPASGIGMAFEKPKKKNMPTLRPEELPKLMYSLSMSNLSVQTRCLIEW
QLLTLVRPSEASGTQWKEIDFDAKLWRIPAERMKAKREHIVPLSPKALDILEVMKTISAHREYVFPSRNDPQKPMNSQTA
NAALKRIGYGGKLVAHGLRSIASTAMNEAEFNADVIESALAHSDKNEVRKAYNRSTYLEQRKNLMNWWSSFVHKSLSF

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=393, Percent_Identity=59.2875318066158, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1788690, Length=391, Percent_Identity=31.2020460358056, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI145693166, Length=401, Percent_Identity=34.6633416458853, Blast_Score=209, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 45385; Mature: 45254

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTTRPLTNTELSRAKATAKDLTLHDGNGLFLLVKTNGRKLWRFRYQKPVTKQRTMIGL
CCCCCCCCCCHHHHHHHHHHHHCEEECCCCEEEEEECCCCEEEHHHHCCCCCHHHHEEEC
GSFPALSLAEARNTRAEYLSLLARGIDPQIYSEQVTVQQQIAEDSIFSNVASKWFAIKRN
CCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
NVTPDYAKDVWRSLERDIFPTIGEIPVQKIKARTLIETLEPIKARGALETVRRLVQRINE
CCCCHHHHHHHHHHHHHHCCCHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IMIYAVNTGLIDANPASGIGMAFEKPKKKNMPTLRPEELPKLMYSLSMSNLSVQTRCLIE
EEEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHEEE
WQLLTLVRPSEASGTQWKEIDFDAKLWRIPAERMKAKREHIVPLSPKALDILEVMKTISA
EEHHHEECCCCCCCCCCEECCCCHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
HREYVFPSRNDPQKPMNSQTANAALKRIGYGGKLVAHGLRSIASTAMNEAEFNADVIESA
HHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LAHSDKNEVRKAYNRSTYLEQRKNLMNWWSSFVHKSLSF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRTTRPLTNTELSRAKATAKDLTLHDGNGLFLLVKTNGRKLWRFRYQKPVTKQRTMIGL
CCCCCCCCCHHHHHHHHHHHHCEEECCCCEEEEEECCCCEEEHHHHCCCCCHHHHEEEC
GSFPALSLAEARNTRAEYLSLLARGIDPQIYSEQVTVQQQIAEDSIFSNVASKWFAIKRN
CCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
NVTPDYAKDVWRSLERDIFPTIGEIPVQKIKARTLIETLEPIKARGALETVRRLVQRINE
CCCCHHHHHHHHHHHHHHCCCHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IMIYAVNTGLIDANPASGIGMAFEKPKKKNMPTLRPEELPKLMYSLSMSNLSVQTRCLIE
EEEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHEEE
WQLLTLVRPSEASGTQWKEIDFDAKLWRIPAERMKAKREHIVPLSPKALDILEVMKTISA
EEHHHEECCCCCCCCCCEECCCCHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
HREYVFPSRNDPQKPMNSQTANAALKRIGYGGKLVAHGLRSIASTAMNEAEFNADVIESA
HHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LAHSDKNEVRKAYNRSTYLEQRKNLMNWWSSFVHKSLSF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]