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The map label for this gene is tpiA2 [H]

Identifier: 218547841

GI number: 218547841

Start: 442697

End: 443488

Strand: Direct

Name: tpiA2 [H]

Synonym: EFER_0431

Alternate gene names: 218547841

Gene position: 442697-443488 (Clockwise)

Preceding gene: 218547840

Following gene: 218547842

Centisome position: 9.65

GC content: 57.83

Gene sequence:

>792_bases
ATGGCAGTGAACCTCACTCTCGGCGTGAGCCTGAAAATGTATTTCGGTTATGCGCAAACCCTTGACTGGAGTCGGCAGGT
AGCGGAAATCGTGCGGCAACATCCGCTGTTAAAAGCATACCCGCAAACCACCTTATTTACCTTTCCCTCTGCGCCGGTCA
TTGACGGTACACTGGGCGCGTTCGCTGGTACGGCAATGCAGGTTGGAGCGCAGAATATTGCCCCCGCAGCGCCTGGCGCC
TGGACTGGAGAAACCAGTGCGGCGATGGTTGCCGAAATGGGCGGGCGATTTGCCGAAATTGGTCACGCTGAACGGCGTCG
TCATTTTCATGAGGATGACGCGGTAATTTGCCGCAAAGTGCAGTTGGCGCTGGAAAACGGCCTGACGCCGGTTATCTGTA
TCGGTGAGCCGCAGCAACTGCCTGTTGCTGATGCCACCGACTTCGCCATTCAGGAGGCGAACGTCATCATTCACTTTCTG
CAACAGCTTGGGCTTAGCGGGGATTTGATTTTTGCCTGGGAGCCGCAGTGGGCCATAGGCGCGCCGCAGCCTGCCAGCGC
AGAATATATCCAGTCCGTCTGTGCGGGACTGCGCAGTTATTTGTCTACTGTTGCGGGTCATCACCGCGTGATTTACGGTG
GCAGCGCCGGACCGGGGTTACTGAGCGAGCTGTGGCCGGATGTCGATGGCCTGTTCCTCGGGCGTTTCGCCCATCAGCCT
GCTGCAATAGCGGCGATCCTGGATGAGGCCTATACCCTGTTAACGCGCAGCGAGACGAAGGAGCATCTGTGA

Upstream 100 bases:

>100_bases
TGGGAACGCCGGATGCGGGCGCCGTCTCGCTGGCGATGATTGTTACGGCAGTGGGCGGCTGTTTTACCGAAGCGGCTGGT
CGTGAGAAAGAGGGGGCGGT

Downstream 100 bases:

>100_bases
AAACTGGACTCAGCACCTGGGCCTATTTCTGGCAGTTATCAGAAAAAGCGCCCAAACCTCTCAGCCTGTTCGACGCCATT
GACGACAGCCAGCGCCTTGG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM 2; Triose-phosphate isomerase 2 [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MAVNLTLGVSLKMYFGYAQTLDWSRQVAEIVRQHPLLKAYPQTTLFTFPSAPVIDGTLGAFAGTAMQVGAQNIAPAAPGA
WTGETSAAMVAEMGGRFAEIGHAERRRHFHEDDAVICRKVQLALENGLTPVICIGEPQQLPVADATDFAIQEANVIIHFL
QQLGLSGDLIFAWEPQWAIGAPQPASAEYIQSVCAGLRSYLSTVAGHHRVIYGGSAGPGLLSELWPDVDGLFLGRFAHQP
AAIAAILDEAYTLLTRSETKEHL

Sequences:

>Translated_263_residues
MAVNLTLGVSLKMYFGYAQTLDWSRQVAEIVRQHPLLKAYPQTTLFTFPSAPVIDGTLGAFAGTAMQVGAQNIAPAAPGA
WTGETSAAMVAEMGGRFAEIGHAERRRHFHEDDAVICRKVQLALENGLTPVICIGEPQQLPVADATDFAIQEANVIIHFL
QQLGLSGDLIFAWEPQWAIGAPQPASAEYIQSVCAGLRSYLSTVAGHHRVIYGGSAGPGLLSELWPDVDGLFLGRFAHQP
AAIAAILDEAYTLLTRSETKEHL
>Mature_262_residues
AVNLTLGVSLKMYFGYAQTLDWSRQVAEIVRQHPLLKAYPQTTLFTFPSAPVIDGTLGAFAGTAMQVGAQNIAPAAPGAW
TGETSAAMVAEMGGRFAEIGHAERRRHFHEDDAVICRKVQLALENGLTPVICIGEPQQLPVADATDFAIQEANVIIHFLQ
QLGLSGDLIFAWEPQWAIGAPQPASAEYIQSVCAGLRSYLSTVAGHHRVIYGGSAGPGLLSELWPDVDGLFLGRFAHQPA
AIAAILDEAYTLLTRSETKEHL

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI226529917, Length=249, Percent_Identity=31.3253012048193, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI4507645, Length=249, Percent_Identity=31.3253012048193, Blast_Score=98, Evalue=7e-21,
Organism=Escherichia coli, GI1790353, Length=195, Percent_Identity=35.3846153846154, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17536593, Length=211, Percent_Identity=34.5971563981043, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320255, Length=243, Percent_Identity=30.4526748971193, Blast_Score=112, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28572004, Length=186, Percent_Identity=35.4838709677419, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI28572008, Length=177, Percent_Identity=36.7231638418079, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI28572006, Length=177, Percent_Identity=36.7231638418079, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 28276; Mature: 28144

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVNLTLGVSLKMYFGYAQTLDWSRQVAEIVRQHPLLKAYPQTTLFTFPSAPVIDGTLGA
CEEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCCCHHHH
FAGTAMQVGAQNIAPAAPGAWTGETSAAMVAEMGGRFAEIGHAERRRHFHEDDAVICRKV
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCHHHHHHHCCCCHHHHHHHH
QLALENGLTPVICIGEPQQLPVADATDFAIQEANVIIHFLQQLGLSGDLIFAWEPQWAIG
HHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
APQPASAEYIQSVCAGLRSYLSTVAGHHRVIYGGSAGPGLLSELWPDVDGLFLGRFAHQP
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCCHHHHHHHHCCH
AAIAAILDEAYTLLTRSETKEHL
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVNLTLGVSLKMYFGYAQTLDWSRQVAEIVRQHPLLKAYPQTTLFTFPSAPVIDGTLGA
EEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCCCHHHH
FAGTAMQVGAQNIAPAAPGAWTGETSAAMVAEMGGRFAEIGHAERRRHFHEDDAVICRKV
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCHHHHHHHCCCCHHHHHHHH
QLALENGLTPVICIGEPQQLPVADATDFAIQEANVIIHFLQQLGLSGDLIFAWEPQWAIG
HHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
APQPASAEYIQSVCAGLRSYLSTVAGHHRVIYGGSAGPGLLSELWPDVDGLFLGRFAHQP
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCCHHHHHHHHCCH
AAIAAILDEAYTLLTRSETKEHL
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]