Click here to switch to the map view.

The map label for this gene is csiR

Identifier: 218547821

GI number: 218547821

Start: 417981

End: 418643

Strand: Reverse

Name: csiR

Synonym: EFER_0408

Alternate gene names: 218547821

Gene position: 418643-417981 (Counterclockwise)

Preceding gene: 218547822

Following gene: 218547818

Centisome position: 9.12

GC content: 52.94

Gene sequence:

>663_bases
ATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATT
ACGGATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTGCGGGAAGCGCTTTCGCAACTGGTAGCAGAAC
GACTGGTCACGGTGGTGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCC
CGCGCCAATATGGAAGCGATGCTGGTCAGTCTGGCGATTGCCCGCGGTGGCGATGAATGGGAGGCAGACGTTCTCGCAAA
AGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGTGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGT
TTCATACTGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGT
TATCGGTTCATCTGGCTACGACGAACAGTGCTTTCGGTGGAAATGCTGGAAGATAAACATGATCAGCACCAGACCCTGAC
TGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCC
AGCAGGCGATGGCTGGCAATTAA

Upstream 100 bases:

>100_bases
TAATCTGTACCGTGCCGATTATGGCGCGCTGGAAAAAACTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTAA
TAATGATCAGGAGTCACACC

Downstream 100 bases:

>100_bases
CTACTCTTCAGGAATACGTAACACCTGCCCCGGATAAATTTTATCCGGGCTTTTCAGCATCGGTTTATTCGCTTCGAAGA
TTTTATTGTACAGATTGGCG

Product: DNA-binding transcriptional regulator CsiR

Products: NA

Alternate protein names: Carbon starvation induced regulator

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLREALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDA
RANMEAMLVSLAIARGGDEWEADVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMAGN

Sequences:

>Translated_220_residues
MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLREALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDA
RANMEAMLVSLAIARGGDEWEADVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMAGN
>Mature_219_residues
TITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLREALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDAR
ANMEAMLVSLAIARGGDEWEADVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAARY
RFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMAGN

Specific function: Modulates expression of csiD and lhgO in a temporal manner during entry into stationary phase or during the first few hours of carbon starvation

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI87082149, Length=220, Percent_Identity=100, Blast_Score=450, Evalue=1e-128,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): CSIR_ECOLI (P37338)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   M88334
- RefSeq:   AP_003237.1
- RefSeq:   NP_417150.4
- ProteinModelPortal:   P37338
- SMR:   P37338
- DIP:   DIP-12095N
- IntAct:   P37338
- STRING:   P37338
- EnsemblBacteria:   EBESCT00000004602
- EnsemblBacteria:   EBESCT00000015662
- GeneID:   948055
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2639
- KEGG:   eco:b2664
- EchoBASE:   EB2287
- EcoGene:   EG12386
- eggNOG:   COG1802
- GeneTree:   EBGT00050000010774
- HOGENOM:   HBG700007
- OMA:   HKAFHTA
- ProtClustDB:   PRK11534
- BioCyc:   EcoCyc:EG12386-MONOMER
- Genevestigator:   P37338
- GO:   GO:0005622
- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00895
- SMART:   SM00345

Pfam domain/function: PF07729 FCD; PF00392 GntR

EC number: NA

Molecular weight: Translated: 24991; Mature: 24860

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLREALSQLVAERLVTVVN
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
QKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEADVLAKAHLLSKLEACDAS
CCCCEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHH
EKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAARYRFIWLRRTVLSVEMLEDKH
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMAGN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLREALSQLVAERLVTVVN
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
QKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEADVLAKAHLLSKLEACDAS
CCCCEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHH
EKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAARYRFIWLRRTVLSVEMLEDKH
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMAGN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428