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The map label for this gene is emrA [H]

Identifier: 218547799

GI number: 218547799

Start: 399862

End: 401034

Strand: Reverse

Name: emrA [H]

Synonym: EFER_0386

Alternate gene names: 218547799

Gene position: 401034-399862 (Counterclockwise)

Preceding gene: 218547800

Following gene: 218547798

Centisome position: 8.74

GC content: 52.6

Gene sequence:

>1173_bases
ATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTGCTCCTCCTTCT
CACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCATTTCGAAGAAACCGATG
ACGCATATGTGGCAGGGAATCAGATTCAAATTATGTCTCAGGTGTCTGGCAGCGTGACGAAAGTCTGGGCCGATAACACC
GATTTTGTAAAAGAAGGCGATGTGCTGGTCACTCTCGACCCGACAGATGCTCGCCAGGCGTTTGAAAAAGCCAAAACGGC
ACTGGCTTCCAGCGTTCGCCAAACCCACCAACTGATGATTAACAGCAAACAGTTGCAGGCGAATATTGAGGTGCAGAAAA
TCGCCCTCGCGAAAGCACAAAGCGACTACAACCGTCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTG
CAACATGCCCGCGACGCGGTGATCAGTGCCCAGGCGCAATTGGATGTCGCCATTCAACAATACAATGCCAATCAGGCGAT
GATTCTGGGGACTAAACTGGAAGATCAGCCTGCCGTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGG
AGCGTACCCGTATTGTCAGTCCGATGACCGGTTATGTCTCCCGCCGCGCAGTACAGCCTGGCGCGCAAATTAGCCCAACG
ACGCCGCTGATGGCGGTCGTTCCAGCCACCAATATGTGGGTGGATGCCAACTTTAAAGAGACGCAGATTGCCAATATGCG
TATCGGTCAGCCGGTCACCATCACCACCGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATA
TGGGGACAGGTAGCGCGTTCTCGCTGCTTCCGGCGCAAAATGCCACTGGTAACTGGATAAAAGTCGTTCAGCGTCTGCCT
GTACGTATTGAACTGGACCAAAAACAGCTGGAGCAATACCCGCTGCGTATCGGTTTATCCACCCTGGTGAGCGTCAATAC
CACTAACCGTGACGGTCAGGTACTGGCAAATAAAGTACGTTCCACACCGGTAGCGGTAAGCACCGCGCGTGAAATCAGCC
TGGCACCTGTCAATAAACTGATCGACGATATCGTAAAAGCTAACGCTGGCTAA

Upstream 100 bases:

>100_bases
TTTATAACAGAAAAAATGACTGGCCAGCATCGCAACATGCTGGCCTTTTTGGCAAGCAGGTCGGCTCAGCCGATGAGTTA
AGAAGATCGTGGAGAACAAT

Downstream 100 bases:

>100_bases
TCCAGAGGTGCGTGTAATGCAACAGCAAAAACCGCTGGAAGGCGCGCAACTGGTCATTATGACGATTGCGCTGTCACTGG
CGACATTCATGCAGGTGCTG

Product: multidrug efflux system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFLVLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNT
DFVKEGDVLVTLDPTDARQAFEKAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
QHARDAVISAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRIVSPMTGYVSRRAVQPGAQISPT
TPLMAVVPATNMWVDANFKETQIANMRIGQPVTITTDIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLP
VRIELDQKQLEQYPLRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG

Sequences:

>Translated_390_residues
MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFLVLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNT
DFVKEGDVLVTLDPTDARQAFEKAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
QHARDAVISAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRIVSPMTGYVSRRAVQPGAQISPT
TPLMAVVPATNMWVDANFKETQIANMRIGQPVTITTDIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLP
VRIELDQKQLEQYPLRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG
>Mature_389_residues
SANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFLVLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTD
FVKEGDVLVTLDPTDARQAFEKAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREELQ
HARDAVISAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRIVSPMTGYVSRRAVQPGAQISPTT
PLMAVVPATNMWVDANFKETQIANMRIGQPVTITTDIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPV
RIELDQKQLEQYPLRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=390, Percent_Identity=99.4871794871795, Blast_Score=789, Evalue=0.0,
Organism=Escherichia coli, GI1788711, Length=378, Percent_Identity=49.7354497354497, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1790519, Length=310, Percent_Identity=30, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI87081951, Length=314, Percent_Identity=27.3885350318471, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789637, Length=317, Percent_Identity=26.4984227129338, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42735; Mature: 42603

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFLVLRHFEETDDAYVAGN
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
QIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFEKAKTALASSVRQTHQLMI
EEEEEEHHCCCEEEEECCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
NSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREELQHARDAVISAQAQLDVAIQQ
CCHHHEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEC
YNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRIVSPMTGYVSRRAVQPGAQISPT
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
TPLMAVVPATNMWVDANFKETQIANMRIGQPVTITTDIYGDDVKYTGKVVGLDMGTGSAF
CCEEEEECCCCEEEECCCCCCEEECEECCCCEEEEEECCCCCCEECCEEEEEECCCCCCE
SLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYPLRIGLSTLVSVNTTNRDGQVLANKVR
EEECCCCCCCHHHHHHHHCCCEEEECHHHHHHCCHHCCCCCEEEEECCCCCHHHHHHHHH
STPVAVSTAREISLAPVNKLIDDIVKANAG
CCCEEEEHHHHCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFLVLRHFEETDDAYVAGN
CCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
QIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFEKAKTALASSVRQTHQLMI
EEEEEEHHCCCEEEEECCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
NSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREELQHARDAVISAQAQLDVAIQQ
CCHHHEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEC
YNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRIVSPMTGYVSRRAVQPGAQISPT
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
TPLMAVVPATNMWVDANFKETQIANMRIGQPVTITTDIYGDDVKYTGKVVGLDMGTGSAF
CCEEEEECCCCEEEECCCCCCEEECEECCCCEEEEEECCCCCCEECCEEEEEECCCCCCE
SLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYPLRIGLSTLVSVNTTNRDGQVLANKVR
EEECCCCCCCHHHHHHHHCCCEEEECHHHHHHCCHHCCCCCEEEEECCCCCHHHHHHHHH
STPVAVSTAREISLAPVNKLIDDIVKANAG
CCCEEEEHHHHCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]