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The map label for this gene is hypF [H]

Identifier: 218547780

GI number: 218547780

Start: 376645

End: 378897

Strand: Direct

Name: hypF [H]

Synonym: EFER_0366

Alternate gene names: 218547780

Gene position: 376645-378897 (Clockwise)

Preceding gene: 218547779

Following gene: 218547783

Centisome position: 8.21

GC content: 57.35

Gene sequence:

>2253_bases
ATGGCAAAAAACACATCTTGCGGTGTCCAACTGCGTATTCGTGGCAAAGTGCAGGGCGTCGGTTTTCGTCCCTTTGTCTG
GCAACTGGCACAGCAATTAAATCTTCACGGCGATGTCTGTAATGACGGCGATGGCGTAGAAGTCCGGCTGCTGGAAGACC
CGGAAACGTTTCTTGTTCAATTGCATCAGCACTGCCCGCCGCTGGCGCGTATTGATAGCGTCGAATGTGAGCCGTTTATC
TGGATGCAGCCGCCCACTGAGTTCACCATCCGCCAGAGCGCGGGCGGCGTCATGAATACGCAAATTGTTCCCGATGCCGC
TACTTGCCCTGCCTGTCTTGCCGAAATGAATACCCCAGGCGAACGGCGTTATCGTTATCCGTTTATCAACTGTACCCACT
GCGGCCCGCGTTTCACCATTATTCGCGCGATGCCTTACGACCGTCCATTTACCGTGATGGCGGCGTTTCCGCTTTGTCCG
GCCTGCGATAAAGAATATCGCGACCCGCTCGATCGTCGCTTCCACGCCCAGCCGGTGGCCTGCCCGGAGTGTGGCCCACA
TCTTGAATGGCTAAGTCATGGTGAACATGCGAAACAAGAGGCGGCATTACAGGCGGCTATCGCTCAGTTAAAAATGGGCA
ACATTGTCGCTATCAAAGGGATTGGCGGATTTCATCTCGCCTGCGATGCGCGTAACAGTAGCGCGGTGGCGACACTACGG
GCGCGCAAACATCGCCCGGCGAAACCGCTGGCGGTTATGTTGCCAACGGCAGAAGGTTTGCCCGACGCTGCCCGTCAGTT
GCTCACCACGCCCGCCGCGCCGATTGTACTGGTGGATAAAATATATGTTCCGGAACTTTGTGACGATATCGCCCCTGGCC
TTAACGAAGTCGGGGTGATGTTGCCTGCGAATCCGCTCCAGCATTTGCTGTTACAGGAACTGCAATGCCCGCTGGTGATG
ACCTCCGGCAACCTGAGCGGTAAACCACCGGCTATCAGCAACGAACAGGCGCTGGAGGACTTGCAGGACATTGCCGACGG
ATTCTTGATTCATAACCGCGACATCGTGCAGCGGATGGATGATTCAGTGGTGCGCGAAAGTGGCGAAATGTTGCGCCGCT
CGCGGGGGTATGTACCGGATGCGCTGGCTTTGCCTCCGGGCTTTAAAAATATTCCTCCTGTGCTGTGTCTCGGTGCGGAT
CTGAAAAATACCTTCTGTCTGGTGCGTGGTGAACAGGCGGTGTTGAGTCAACATTTGGGCGATTTAAGTGACGATGGCAT
TCAGATGCAGTGGCGCGAAGCGTTGCGCCTGATGCAAAACATCTACGATTTTACTCCACAGTTTGTGGTGCATGATGCGC
ATCCGGGCTATGTCTCCAGCCAGTGGGCGCGTGAGATGAATCTGCCGACGCAAACGGTGCTGCATCACCATGCCCACGCG
GCGGCGTGTCTGGCAGAACATCACTGGCCGCTGGATGGCAGTGATGTCATTGCGTTGACGCTCGACGGTATTGGTATGGG
TGAGAACGGCGCTTTGTGGGGCGGCGAGTGCTTGAGGGTGAACTATCGCGAATGCCAGCACCTGGGCGGCTTGCCTGCGG
TGGCGCTTCCGGGGGGCGATCTTGCGGCAAAGCAGCCGTGGCGTAACCTGCTGGCGCAGTGCCTGCGCTTTGTGCCGGAG
TGGCAGAATTACCCTGAAACGGTAAGTGTGCAACAGCAAAACTGGAGCGTGCTGGCGCGGGCCATTGAGCGTGGAATCAA
TGCGCCGCTGGCATCATCGTGTGGGCGTTTGTTCGATGCTGTGGCGGCGGCACTGGGCTGTGCGCCAGCTACGTTAAGTT
ATGAAGGTGAAGCGGCTTGTGCTCTGGAGGCGCTCGCAGCTTCGTGCCAGGGAGTGACGCATCCGGTGACGATGCCGCTG
GTGGACAATCAACTGGATCTCGCCACTTTCTGGCAGCAGTGGCTGAACTGGCAGGCACCGGTTAATCAACGCGCGTGGGC
ATTTCATGATGCGCTGGCGCAGGGTTTTGCCGCGTTGATGCGTGAGCAGGCCACGATGCGTGGTATCACTACGTTGGTAT
TTAGCGGCGGGGTTATTCATAACCGTTTGCTGCGTGCACGCCTAGCGCATTATCTTGCTGATTTTACGTTGCTCTTCCCG
CAGAGTTTACCGGCGGGGGATGGCGGGTTGTCTCTGGGGCAGGGGGTTATTGTTGCGGCCCGTTGGGTGGCGGGTGAAGT
CCAGAATGGATAA

Upstream 100 bases:

>100_bases
AATACGGAGCGGGCGGTATCGCCAACCTGTGACTACCGGATGCGACGCGGTCGTGTCCGGCTGGGCGGGTTACGCAATTC
ATGATGGCGGGAATAACTTA

Downstream 100 bases:

>100_bases
ACGTGTTGCATTTGGGCGCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACACATTCGCTCCCCGTAGGTCGGAT
AAGACGCGCCAGCGTCGCAT

Product: carbamoyl phosphate phosphatase

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 750; Mature: 749

Protein sequence:

>750_residues
MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEDPETFLVQLHQHCPPLARIDSVECEPFI
WMQPPTEFTIRQSAGGVMNTQIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
ACDKEYRDPLDRRFHAQPVACPECGPHLEWLSHGEHAKQEAALQAAIAQLKMGNIVAIKGIGGFHLACDARNSSAVATLR
ARKHRPAKPLAVMLPTAEGLPDAARQLLTTPAAPIVLVDKIYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVM
TSGNLSGKPPAISNEQALEDLQDIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNIPPVLCLGAD
LKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQFVVHDAHPGYVSSQWAREMNLPTQTVLHHHAHA
AACLAEHHWPLDGSDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPE
WQNYPETVSVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEALAASCQGVTHPVTMPL
VDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFP
QSLPAGDGGLSLGQGVIVAARWVAGEVQNG

Sequences:

>Translated_750_residues
MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEDPETFLVQLHQHCPPLARIDSVECEPFI
WMQPPTEFTIRQSAGGVMNTQIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
ACDKEYRDPLDRRFHAQPVACPECGPHLEWLSHGEHAKQEAALQAAIAQLKMGNIVAIKGIGGFHLACDARNSSAVATLR
ARKHRPAKPLAVMLPTAEGLPDAARQLLTTPAAPIVLVDKIYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVM
TSGNLSGKPPAISNEQALEDLQDIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNIPPVLCLGAD
LKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQFVVHDAHPGYVSSQWAREMNLPTQTVLHHHAHA
AACLAEHHWPLDGSDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPE
WQNYPETVSVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEALAASCQGVTHPVTMPL
VDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFP
QSLPAGDGGLSLGQGVIVAARWVAGEVQNG
>Mature_749_residues
AKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEDPETFLVQLHQHCPPLARIDSVECEPFIW
MQPPTEFTIRQSAGGVMNTQIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPA
CDKEYRDPLDRRFHAQPVACPECGPHLEWLSHGEHAKQEAALQAAIAQLKMGNIVAIKGIGGFHLACDARNSSAVATLRA
RKHRPAKPLAVMLPTAEGLPDAARQLLTTPAAPIVLVDKIYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMT
SGNLSGKPPAISNEQALEDLQDIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNIPPVLCLGADL
KNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQFVVHDAHPGYVSSQWAREMNLPTQTVLHHHAHAA
ACLAEHHWPLDGSDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEW
QNYPETVSVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEALAASCQGVTHPVTMPLV
DNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQ
SLPAGDGGLSLGQGVIVAARWVAGEVQNG

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=750, Percent_Identity=96.1333333333333, Blast_Score=1392, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 81974; Mature: 81843

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEDPETFLVQ
CCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHH
LHQHCPPLARIDSVECEPFIWMQPPTEFTIRQSAGGVMNTQIVPDAATCPACLAEMNTPG
HHHHCCCCHHCCCCCCCEEEEECCCCCEEEECCCCCEEECEECCCCHHHHHHHHHCCCCC
ERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPACDKEYRDPLDRRFHAQPVA
CCEEECCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCHHHCCHHHHHHCCCCCC
CPECGPHLEWLSHGEHAKQEAALQAAIAQLKMGNIVAIKGIGGFHLACDARNSSAVATLR
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCHHHHHH
ARKHRPAKPLAVMLPTAEGLPDAARQLLTTPAAPIVLVDKIYVPELCDDIAPGLNEVGVM
HHCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCHHHCCEE
LPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALEDLQDIADGFLIHNRDIVQRMD
CCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCEEEECHHHHHHHH
DSVVRESGEMLRRSRGYVPDALALPPGFKNIPPVLCLGADLKNTFCLVRGEQAVLSQHLG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCHHHHHHHCC
DLSDDGIQMQWREALRLMQNIYDFTPQFVVHDAHPGYVSSQWAREMNLPTQTVLHHHAHA
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHH
AACLAEHHWPLDGSDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVALPGGD
HHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCCEEEECHHHHHHCCCCCEEEECCCC
LAAKQPWRNLLAQCLRFVPEWQNYPETVSVQQQNWSVLARAIERGINAPLASSCGRLFDA
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VAAALGCAPATLSYEGEAACALEALAASCQGVTHPVTMPLVDNQLDLATFWQQWLNWQAP
HHHHHCCCCCEECCCCCHHHHHHHHHHHHCCCCCCEEECEECCCCHHHHHHHHHCCCCCC
VNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
QSLPAGDGGLSLGQGVIVAARWVAGEVQNG
CCCCCCCCCCCCCCCCEEEEHHHCCCCCCC
>Mature Secondary Structure 
AKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEDPETFLVQ
CCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHH
LHQHCPPLARIDSVECEPFIWMQPPTEFTIRQSAGGVMNTQIVPDAATCPACLAEMNTPG
HHHHCCCCHHCCCCCCCEEEEECCCCCEEEECCCCCEEECEECCCCHHHHHHHHHCCCCC
ERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPACDKEYRDPLDRRFHAQPVA
CCEEECCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCHHHCCHHHHHHCCCCCC
CPECGPHLEWLSHGEHAKQEAALQAAIAQLKMGNIVAIKGIGGFHLACDARNSSAVATLR
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCHHHHHH
ARKHRPAKPLAVMLPTAEGLPDAARQLLTTPAAPIVLVDKIYVPELCDDIAPGLNEVGVM
HHCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCHHHCCEE
LPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALEDLQDIADGFLIHNRDIVQRMD
CCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCEEEECHHHHHHHH
DSVVRESGEMLRRSRGYVPDALALPPGFKNIPPVLCLGADLKNTFCLVRGEQAVLSQHLG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCHHHHHHHCC
DLSDDGIQMQWREALRLMQNIYDFTPQFVVHDAHPGYVSSQWAREMNLPTQTVLHHHAHA
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHH
AACLAEHHWPLDGSDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVALPGGD
HHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCCEEEECHHHHHHCCCCCEEEECCCC
LAAKQPWRNLLAQCLRFVPEWQNYPETVSVQQQNWSVLARAIERGINAPLASSCGRLFDA
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VAAALGCAPATLSYEGEAACALEALAASCQGVTHPVTMPLVDNQLDLATFWQQWLNWQAP
HHHHHCCCCCEECCCCCHHHHHHHHHHHHCCCCCCEEECEECCCCHHHHHHHHHCCCCCC
VNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
QSLPAGDGGLSLGQGVIVAARWVAGEVQNG
CCCCCCCCCCCCCCCCEEEEHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8661925; 12206761 [H]