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The map label for this gene is ascG [H]

Identifier: 218547778

GI number: 218547778

Start: 374803

End: 375816

Strand: Direct

Name: ascG [H]

Synonym: EFER_0363

Alternate gene names: 218547778

Gene position: 374803-375816 (Clockwise)

Preceding gene: 218547775

Following gene: 218547779

Centisome position: 8.17

GC content: 52.47

Gene sequence:

>1014_bases
ATGATGACGACGATGCTGGAAGTGGCGAAGCGCGCCGGGGTTTCAAAAGCGACCGTTTCCCGCGTGCTTTCAGGTAATGG
CTACGTCAGCCAGGAAACTAAAGATCGCGTGTTTCAGGCGGTGGAAGAGAGCGGCTACCGCCCAAATTTGCTGGCGCGCA
ATCTGTCAGCGAAGAGTACCCAGACGCTGGGGCTGGTAGTGACCAACACGCTTTACCACGGCATTTATTTTAGTGAATTA
CTGTTTCATACCGCGAGAATGGCGGAAGAGAAAGGGCGTCAGCTGCTATTGGCAGATGGCAAACATAGCGCCGAAGAAGA
GCGCCAGGCGATTCAGTATCTGCTGGATCTGCGCTGCGACGCCATTATGATTTACCCGCGCTTTTTAAACGTGGATGAGA
TTGATGACATCATTGACGCACAAAGTCAGCCGATAATGGTGCTCAATCGCCGCCTGCGCAAAAACAGCAGCCATAGCGTC
TGGTGCGATCATAAACAGACCAGCTTTAACGCCGTGGCAGAGTTGATAAACGCCGGACATCAGGAGATTGCTTTCCTTAC
CGGTTCGATGGATTCCCCCACCAGCATTGAACGCCTTGCCGGATATAAAGACGCGCTGGCGCAGCATGGTATTGCACTCA
ATGAAAAACTTATCGCTAACGGTAAATGGACGCCTGCCAGCGGGGCCGAAGGGGTAGAAACGTTGCTCGAACGTGGGGCT
AAATTTAGCGCGTTAGTTGCCAGTAACGACGATATGGCGATAGGTGCGATGAAAGCGTTGCATGACCGTGGTGTAGCGGT
GCCGGGGCAAGTGTCGGTTATTGGATTTGATGATATCGCAATTGCCCCCTACACCGTTCCGGCGCTCTCCAGCGTAAAAA
TTCCGGTGACTGAGATGATTCAGGAAATAATTGGGCGGCTGATATTTATGCTCGATGGTGGTGCTTACACACCGCCGAAA
ACCTTCAGCGGAAAACTGATCCGCCGCGACTCCCTCATTGATCTTTCGCGATAA

Upstream 100 bases:

>100_bases
TTTGTGAAACCGGTCACCTGAATATTTTTCCCGCTGCTTGCGCAAGGACAATCAGCCAACGGTGCAGTACACTGCGTTGA
TTGCGTGTAAAGGGATGAAA

Downstream 100 bases:

>100_bases
GCATTGTCATCACATGTCATCGTCACTAGTGTCGATGACATCGTTTTTTCTGCATTGCAGAATTTAATTTCTTTAAATAA
CAATCAATTAAAAGTTGGCA

Product: DNA-binding transcriptional repressor

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSEL
LFHTARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLNVDEIDDIIDAQSQPIMVLNRRLRKNSSHSV
WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIANGKWTPASGAEGVETLLERGA
KFSALVASNDDMAIGAMKALHDRGVAVPGQVSVIGFDDIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPK
TFSGKLIRRDSLIDLSR

Sequences:

>Translated_337_residues
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSEL
LFHTARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLNVDEIDDIIDAQSQPIMVLNRRLRKNSSHSV
WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIANGKWTPASGAEGVETLLERGA
KFSALVASNDDMAIGAMKALHDRGVAVPGQVSVIGFDDIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPK
TFSGKLIRRDSLIDLSR
>Mature_337_residues
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSEL
LFHTARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLNVDEIDDIIDAQSQPIMVLNRRLRKNSSHSV
WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIANGKWTPASGAEGVETLLERGA
KFSALVASNDDMAIGAMKALHDRGVAVPGQVSVIGFDDIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPK
TFSGKLIRRDSLIDLSR

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=337, Percent_Identity=96.7359050445104, Blast_Score=671, Evalue=0.0,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=34.2342342342342, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=35.6287425149701, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1787948, Length=299, Percent_Identity=29.4314381270903, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790369, Length=311, Percent_Identity=30.2250803858521, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787580, Length=331, Percent_Identity=28.7009063444109, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1790194, Length=307, Percent_Identity=27.6872964169381, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=28.0597014925373, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787906, Length=317, Percent_Identity=28.7066246056782, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790715, Length=295, Percent_Identity=24.7457627118644, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI48994940, Length=317, Percent_Identity=25.5520504731861, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789456, Length=314, Percent_Identity=28.343949044586, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786268, Length=314, Percent_Identity=24.8407643312102, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36923; Mature: 36923

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKST
CCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
QTLGLVVTNTLYHGIYFSELLFHTARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCC
AIMIYPRFLNVDEIDDIIDAQSQPIMVLNRRLRKNSSHSVWCDHKQTSFNAVAELINAGH
EEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCC
QEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIANGKWTPASGAEGVETLLERGA
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHCCC
KFSALVASNDDMAIGAMKALHDRGVAVPGQVSVIGFDDIAIAPYTVPALSSVKIPVTEMI
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCHHHHH
QEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIDLSR
HHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKST
CCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
QTLGLVVTNTLYHGIYFSELLFHTARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCC
AIMIYPRFLNVDEIDDIIDAQSQPIMVLNRRLRKNSSHSVWCDHKQTSFNAVAELINAGH
EEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCC
QEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIANGKWTPASGAEGVETLLERGA
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHCCC
KFSALVASNDDMAIGAMKALHDRGVAVPGQVSVIGFDDIAIAPYTVPALSSVKIPVTEMI
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCHHHHH
QEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIDLSR
HHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]