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The map label for this gene is hycD [H]

Identifier: 218547770

GI number: 218547770

Start: 366658

End: 367581

Strand: Direct

Name: hycD [H]

Synonym: EFER_0355

Alternate gene names: 218547770

Gene position: 366658-367581 (Clockwise)

Preceding gene: 218547769

Following gene: 218547771

Centisome position: 7.99

GC content: 57.25

Gene sequence:

>924_bases
ATGAGTGTTTTATATCCGTTAATTCAGGCGCTGGTGTTATTTGCCGTTGCGCCGCTGCTCTCCGGTATTACCCGCGTGGC
GCGCGCCCGCTTGCATAACCGTCGCGGGCCGGGCGTGTTGCAGGAGTATCGCGACATTATCAAACTGCTGGCTCGTCAGA
GCGTTGGTCCCGATGCCTCCGGCTGGGTGTTCCGCCTGACACCGTACGTAATGGTTGGCGTCATGTTGACTATCGCTACC
GCGCTGCCGGTAGTGACTGTCGGTTCTCCGCTGCCGCAACTGGGGGATTTGATCACTTTACTGTATCTCTTCGCCATTGC
GCGTTTCTTCTTTGCCATTTCTGGTCTGGATACCGGTAGTCCGTTTACTGCTATTGGCGCGAGCCGTGAAGCGATGCTGG
GCGTGCTGGTTGAACCGATGCTGCTGCTTGGTCTGTGGGTCGCCGCGCAGGTGGCCGGTTCCACCAACATCAGCAACATC
ACCGACACCGTTTATCACTGGCCGCTGAGCCAGAGCATTCCGCTGATGCTGGCGCTCTGTGCCTGCGCATTCGCCACCTT
TATCGAAATGGGCAAACTGCCGTTCGACCTGGCGGAAGCCGAGCAGGAGCTGCAGGAAGGTCCGCTCTCTGAGTACAGCG
GCAGCGGCTTTGGCGTCATGAAATGGGGTATTAGCCTGAAACAGCTGGTGGTGTTACAGATGTTCGTCGGGGTGTTTATT
CCGTGGGGACAAATGGAAACCTTCACCGCCGGTGGGCTGCTGCTGGCGCTGGTGATTGCCATCGTCAAACTGGTGGTCGG
CGTACTGGTTATCGCGCTATTCGAAAACAGCATGGCCCGTCTGCGTCTTGATATTACTCCACGCATTACCTGGGCTGGTT
TTGGCTTTGCATTTTTAGCGTTCGTCTCCTTGCTGGCGGCGTGA

Upstream 100 bases:

>100_bases
CGGGCTGGCAGTACGAGGGGAGTGCGTTGCTGTTCCGCCGGATGGCGCTGGTTGAACTGGCGGTACTGGTGGTGATTATT
GTTTCACGAGGAGCCTGAGA

Downstream 100 bases:

>100_bases
TTAAAGAGAGTTTGAGCATGTCTGAAGAAAAATTAGGTCAACATTATCTCGCCGCGCTGAATGAGGCATTTCCGGGCGTC
GTGCTGGACCACGCCTGGCA

Product: hydrogenase 3, membrane subunit

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLARQSVGPDASGWVFRLTPYVMVGVMLTIAT
ALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
TDTVYHWPLSQSIPLMLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
PWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLAFVSLLAA

Sequences:

>Translated_307_residues
MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLARQSVGPDASGWVFRLTPYVMVGVMLTIAT
ALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
TDTVYHWPLSQSIPLMLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
PWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLAFVSLLAA
>Mature_306_residues
SVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLARQSVGPDASGWVFRLTPYVMVGVMLTIATA
LPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNIT
DTVYHWPLSQSIPLMLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFIP
WGQMETFTAGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLAFVSLLAA

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=307, Percent_Identity=99.3485342019544, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI87082115, Length=303, Percent_Identity=51.8151815181518, Blast_Score=283, Evalue=7e-78,
Organism=Escherichia coli, GI1788618, Length=306, Percent_Identity=25.8169934640523, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 33076; Mature: 32945

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00667 COMPLEX1_ND1_1 ; PS00668 COMPLEX1_ND1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLARQSVGPDAS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPLSQSIPLMLALC
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHH
ACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCEEHHHHHHHHHH
FVSLLAA
HHHHHCC
>Mature Secondary Structure 
SVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLARQSVGPDAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPLSQSIPLMLALC
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHH
ACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCEEHHHHHHHHHH
FVSLLAA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]