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The map label for this gene is cysN [H]

Identifier: 218547732

GI number: 218547732

Start: 330459

End: 331886

Strand: Direct

Name: cysN [H]

Synonym: EFER_0316

Alternate gene names: 218547732

Gene position: 330459-331886 (Clockwise)

Preceding gene: 218547731

Following gene: 218547733

Centisome position: 7.2

GC content: 53.85

Gene sequence:

>1428_bases
ATGAACACCGTACTTGCACAACAAATCGCCAACGAAGGCGGCGTCGAAGCCTGGATGATTGCGCAACAACATAAAAGCCT
GCTGCGTTTTCTGACCTGTGGTAGCGTCGATGACGGCAAAAGTACCCTGATTGGTCGCCTGCTGCACGATACCCGCCAAA
TCTACGAAGATCAGCTCTCATCGCTGCATAACGACAGTAAACGTCACGGCACCCAGGGCGAAAAACTGGATCTGGCACTG
CTGGTGGATGGCCTGCAAGCTGAGCGTGAACAGGGCATCACCATTGATGTGGCTTACCGCTATTTCTCCACCGAGAAGCG
TAAATTTATTATCGCCGATACCCCTGGACACGAGCAGTACACCCGCAATATGGCGACTGGCGCATCGACATGCGATCTGG
CGATCTTACTGATCGATGCCCGTAAAGGCGTGCTCGATCAAACCCGTCGTCACAGTTTTATCTCCACGCTGTTGGGGATC
AAACATCTGATCGTGGCGATCAACAAAATGGATCTGGTGGATTACAGTGAAGAGACGTTCACCCGCATTCGTGAAGATTA
TCTGACCTTTGCCGAGCAGCTGCCGGGTAATCTGGATATTCGCTTTGTGCCGCTCTCCGCGCTGGAAGGCGACAACGTGG
CTTCGCAAAGTGAAAGCATGTTGTGGTACAGCGGTCCGACACTACTCGAAGTACTGGAAACAGTGGAGATACAGCGAGTG
GTGGATGCCCAGCCAATGCGCTTTCCGGTGCAGTACGTTAACCGCCCGAATCTTGATTTTCGTGGCTACGCCGGAACGCT
GGCATCCGGTCGCGTGCAAGTCGGGCAACGAGTCAAAGTGCTGCCCTCTGGTGTGGAGTCAAACGTCGCGCGGATAGTTA
CTTTTGATGGCGATCGCGAAGAAGCCTTTGCCGGAGAAGCGATCACCCTGGTGCTGACGGATGAGATCGACATCAGCCGT
GGCGATCTGCTGCTGGCGGCAGACGAAGTGTTACCGGCTGTGCAGAGTGCGTCGGTGGCTGTGGTATGGATGGCGGAACA
GCCGCTTTCCCCGGGCCAGAGTTACGACATCAAAATTGCCGGTAAGAAGACGCGTGCTCGTGTTGATGGCATTCGTTATC
AGGTTGATATTAATAACCTTACCCAACGCGAAGTTGAAAATCTGTCACTGAATGGCATCGGCCTCGTGGATCTCACCTTT
GACGAGCCGCTGGTGTTAGATCGCTATCAACAAAATCCGGTAACGGGTGGGCTGATTTTTATCGATCGCCTGAGCAATGT
GACCGTGGGTGCCGGTATGGTGCACGAGCCAGTTAGCCAGGCAACTGCTGCGGCATCTGAATTCAGTGCATTCGAACTGG
AATTGAATGCCCTGGTCCGTCGCCATTTCCCACACTGGGGCGCGCGCGATTTACTGGGAGATAAATAA

Upstream 100 bases:

>100_bases
GAGATGCTGGTTTCCACCACCAGTGAACGTCAGGGGCGCGTGATTGACCGCGACCAGGCGGGATCCATGGAGCTGAAAAA
ACGTCAGGGGTATTTTTAAG

Downstream 100 bases:

>100_bases
TGGCGCTGCATGACGAAAACGTCGTCTGGCATAGCCATCCGGTCACTGTGCAACAACGCGAGCAACACCACGGTCATCGT
GGTGTTGTGTTGTGGTTTAC

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGI
KHLIVAINKMDLVDYSEETFTRIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSESMLWYSGPTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVQVGQRVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISR
GDLLLAADEVLPAVQSASVAVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLSLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAASEFSAFELELNALVRRHFPHWGARDLLGDK

Sequences:

>Translated_475_residues
MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGI
KHLIVAINKMDLVDYSEETFTRIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSESMLWYSGPTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVQVGQRVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISR
GDLLLAADEVLPAVQSASVAVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLSLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAASEFSAFELELNALVRRHFPHWGARDLLGDK
>Mature_475_residues
MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGI
KHLIVAINKMDLVDYSEETFTRIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSESMLWYSGPTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVQVGQRVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISR
GDLLLAADEVLPAVQSASVAVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLSLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAASEFSAFELELNALVRRHFPHWGARDLLGDK

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=29.1954022988506, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=29.1954022988506, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI194018520, Length=293, Percent_Identity=34.4709897610922, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI194018522, Length=336, Percent_Identity=32.1428571428571, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI194097354, Length=293, Percent_Identity=34.4709897610922, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI4503475, Length=441, Percent_Identity=27.6643990929705, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4503471, Length=441, Percent_Identity=27.2108843537415, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI46094014, Length=325, Percent_Identity=32, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI34147630, Length=331, Percent_Identity=29.0030211480363, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI157426893, Length=351, Percent_Identity=25.0712250712251, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1789108, Length=475, Percent_Identity=97.8947368421053, Blast_Score=952, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=326, Percent_Identity=27.3006134969325, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789737, Length=326, Percent_Identity=27.3006134969325, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI2367247, Length=333, Percent_Identity=26.4264264264264, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115532067, Length=429, Percent_Identity=26.3403263403263, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI115532065, Length=426, Percent_Identity=26.5258215962441, Blast_Score=166, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI32566629, Length=426, Percent_Identity=28.6384976525822, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17552884, Length=445, Percent_Identity=29.438202247191, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=445, Percent_Identity=29.438202247191, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=27.6255707762557, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI25141371, Length=241, Percent_Identity=27.3858921161826, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.231884057971, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=348, Percent_Identity=28.1609195402299, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6322937, Length=391, Percent_Identity=30.4347826086957, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6325337, Length=439, Percent_Identity=28.246013667426, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=439, Percent_Identity=28.246013667426, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=428, Percent_Identity=27.1028037383178, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6324761, Length=146, Percent_Identity=34.2465753424658, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=193, Percent_Identity=31.6062176165803, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=28.5057471264368, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI45553807, Length=442, Percent_Identity=26.9230769230769, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45553816, Length=442, Percent_Identity=26.9230769230769, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24651721, Length=442, Percent_Identity=26.9230769230769, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17864154, Length=442, Percent_Identity=26.9230769230769, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24652838, Length=441, Percent_Identity=26.3038548752834, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI17137572, Length=441, Percent_Identity=26.3038548752834, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI17137380, Length=312, Percent_Identity=30.1282051282051, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI281363316, Length=318, Percent_Identity=28.3018867924528, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17864358, Length=318, Percent_Identity=28.3018867924528, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921738, Length=93, Percent_Identity=43.010752688172, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19922690, Length=284, Percent_Identity=26.056338028169, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 52622; Mature: 52622

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
CCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHCCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGIKHLIVAINKMDLVDYSEETF
HHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TRIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSESMLWYSGPTLLEVLETVEIQRV
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHH
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVQVGQRVKVLPSGVESNVARIVTFDGDRE
HCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEECCCEEEECCCCCCCCEEEEEEECCCCC
EAFAGEAITLVLTDEIDISRGDLLLAADEVLPAVQSASVAVVWMAEQPLSPGQSYDIKIA
HHCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEE
GKKTRARVDGIRYQVDINNLTQREVENLSLNGIGLVDLTFDEPLVLDRYQQNPVTGGLIF
CCHHHHEECCEEEEEECCCCCHHHHHHCCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEE
IDRLSNVTVGAGMVHEPVSQATAAASEFSAFELELNALVRRHFPHWGARDLLGDK
EEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure
MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
CCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHCCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGIKHLIVAINKMDLVDYSEETF
HHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
TRIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSESMLWYSGPTLLEVLETVEIQRV
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHH
VDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVQVGQRVKVLPSGVESNVARIVTFDGDRE
HCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEECCCEEEECCCCCCCCEEEEEEECCCCC
EAFAGEAITLVLTDEIDISRGDLLLAADEVLPAVQSASVAVVWMAEQPLSPGQSYDIKIA
HHCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEE
GKKTRARVDGIRYQVDINNLTQREVENLSLNGIGLVDLTFDEPLVLDRYQQNPVTGGLIF
CCHHHHEECCEEEEEECCCCCHHHHHHCCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEE
IDRLSNVTVGAGMVHEPVSQATAAASEFSAFELELNALVRRHFPHWGARDLLGDK
EEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA