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The map label for this gene is gcvA [H]

Identifier: 218547680

GI number: 218547680

Start: 274091

End: 275008

Strand: Direct

Name: gcvA [H]

Synonym: EFER_0259

Alternate gene names: 218547680

Gene position: 274091-275008 (Clockwise)

Preceding gene: 218547679

Following gene: 218547681

Centisome position: 5.97

GC content: 47.82

Gene sequence:

>918_bases
ATGTCAAAACGTTTACCACCCCTCAATGCTCTGCGTGTTTTTGATGCTGCAGCACGACATTTAAGCTTCACGCGAGCAGC
TGATGAGCTTTTTGTGACGCAGGCCGCAGTAAGCCACCAAATCAAGTCTCTTGAGGATTTCCTGGGGTTAAAACTGTTCC
GTCGCCGTAACCGTTCTCTACTTTTAACGGAAGAAGGACAAAGTTACTATCTCGATATCAAAGAGATATTTTCGCAGCTA
ACGGAAGCAACACGTAAACTTCAGGCGCGAAGTGCGAAAGGGGCGTTAACGGTCAGTTTATTACCCAGTTTCGCCATTCA
ATGGTTGGTTCCTCGTCTCTCCAGCTTTAATACAGCTTATCCGGGAATTGATGTCAGAATTCAGGCGGTGGACCGTCAGG
AAGATAAACTGGCGGATGATGTTGATGTGGCAATTTTTTATGGCCGGGGCAACTGGCCAGGTTTACGTGTTGAAAAACTC
TACGCAGAATATCTCCTTCCGGTATGTTCACCTCTGCTTTTGACCGGAGACAAGCCGTTAAAAACGCCTGAAGATCTCGC
TAAACACACCTTATTGCATGATGCCTCTCGCCGTGACTGGCAAACTTACATTCGGCAGTTGGGGTTAAATCATATTAATG
TGCAACAGGGGCCGATTTTCAGCCATAGTGCGATGGTGTTGCAGGCAGCGATTCACGGACAGGGCGTGGCACTGGCGAAC
AATGTTATGGCGCAGTCGGAGATTGAGGCCGGGCGACTTGTTTGCCCATTTAACGATGTTCTGGTCAGTAAGAACGCATT
TTATCTGGTTTGTCATGACAGCCAGGCAGAACTCGGTAAAATAGCGGCTTTCCGCCAGTGGATTCTGGAAAAAGCGGCGA
GTGAACAAGAGAAATTCCGCTTTCGTTACGAACAATAA

Upstream 100 bases:

>100_bases
AGCTCAACGGACAATTTATAATGGCTCAGATTAAAAAAACTAATAGGTTACGTAGTGTAGCCTGATTGTTAAATCTTTTT
AACATCAAAGTCTAAAAGCC

Downstream 100 bases:

>100_bases
CTTACGTAGGGTACTAGCATGACCAGCCGTTTTATGCTGATTTTTGCCGCCATCAGCGGTTTCATTTTTGTTGCTCTGGG
GGCTTTTGGCGCTCATGTCC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSKRLPPLNALRVFDAAARHLSFTRAADELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYYLDIKEIFSQL
TEATRKLQARSAKGALTVSLLPSFAIQWLVPRLSSFNTAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
YAEYLLPVCSPLLLTGDKPLKTPEDLAKHTLLHDASRRDWQTYIRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGVALAN
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILEKAASEQEKFRFRYEQ

Sequences:

>Translated_305_residues
MSKRLPPLNALRVFDAAARHLSFTRAADELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYYLDIKEIFSQL
TEATRKLQARSAKGALTVSLLPSFAIQWLVPRLSSFNTAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
YAEYLLPVCSPLLLTGDKPLKTPEDLAKHTLLHDASRRDWQTYIRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGVALAN
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILEKAASEQEKFRFRYEQ
>Mature_304_residues
SKRLPPLNALRVFDAAARHLSFTRAADELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYYLDIKEIFSQLT
EATRKLQARSAKGALTVSLLPSFAIQWLVPRLSSFNTAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKLY
AEYLLPVCSPLLLTGDKPLKTPEDLAKHTLLHDASRRDWQTYIRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGVALANN
VMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILEKAASEQEKFRFRYEQ

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=305, Percent_Identity=97.3770491803279, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=34.7222222222222, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=30.952380952381, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=28.5223367697594, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=27.972027972028, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI157672245, Length=212, Percent_Identity=31.1320754716981, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081978, Length=257, Percent_Identity=31.5175097276265, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1789639, Length=250, Percent_Identity=26.8, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787128, Length=305, Percent_Identity=23.6065573770492, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1789440, Length=266, Percent_Identity=27.0676691729323, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788887, Length=176, Percent_Identity=33.5227272727273, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693105, Length=143, Percent_Identity=29.3706293706294, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790262, Length=198, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787879, Length=130, Percent_Identity=32.3076923076923, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790783, Length=259, Percent_Identity=28.957528957529, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34481; Mature: 34350

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRLPPLNALRVFDAAARHLSFTRAADELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYYLDIKEIFSQLTEATRKLQARSAKGALTVSLLPSFAIQWLVPRLSSFNTAY
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
PGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKLYAEYLLPVCSPLLLTGDKPL
CCCEEEEEEECCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCHHEECCCCCC
KTPEDLAKHTLLHDASRRDWQTYIRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGVALAN
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCCCCCHHHH
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILEKAASEQEKFR
HHHHHHHCCCCCEECCCHHHEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHH
FRYEQ
CCCCC
>Mature Secondary Structure 
SKRLPPLNALRVFDAAARHLSFTRAADELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYYLDIKEIFSQLTEATRKLQARSAKGALTVSLLPSFAIQWLVPRLSSFNTAY
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCC
PGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKLYAEYLLPVCSPLLLTGDKPL
CCCEEEEEEECCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCHHEECCCCCC
KTPEDLAKHTLLHDASRRDWQTYIRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGVALAN
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCCCCCHHHH
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILEKAASEQEKFR
HHHHHHHCCCCCEECCCHHHEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHH
FRYEQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]