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The map label for this gene is amiC [H]

Identifier: 218547674

GI number: 218547674

Start: 267812

End: 269065

Strand: Direct

Name: amiC [H]

Synonym: EFER_0253

Alternate gene names: 218547674

Gene position: 267812-269065 (Clockwise)

Preceding gene: 218547672

Following gene: 218547675

Centisome position: 5.84

GC content: 50.4

Gene sequence:

>1254_bases
ATGTCAGGAACCAATTCAGCAATCAGCCGTCGTCGCTTATTACAAGGGGCGGGAGCTATGTGGCTATTGAGCGTAAGTCA
GGTGGGACTGGCAGCGGTCAGTCAGGTTGTCGCTGTACGTGTCTGGCCTGCATCGAGTTATACGCGTGTCACAGTAGAAT
CGAGTCGCCTGCTGAAATACAAACAATTTGCGTTGAGTAATCCTGAACGTGTGGTGGTTGATCTCGAAGGCGTAAATCTT
AACTCCGTGCTGAAAGGTGTGGCGGCGCAAATACGAACTGATGATCCTTTCATAAAATCAGCACGGGTAGGGCAGTTTGA
TCCACAAACTGTGCGAATGGTTTTTGAACTCAAGCAAAATGTAAAACCACAGATTTTCGCTTTGGCTCCGGTTGCCGGGT
TTAAAGAGCGTCTGGTGATGGATCTTTACCCAGCCAATGCCCAGGACATGCAGGATCCGCTGCTGGCATTGTTAGAAGAT
TACAACAAAGGCGATCTCGAAAAGCAGGTGCCGCCAGCACAAAGTGGTCCACAACCGGGTAAAGCAGGTCGCGATCGCCC
GATAGTCATCATGCTTGACCCTGGCCACGGCGGCGAAGACTCCGGTGCGGTGGGGAAATACAAAACACGCGAAAAAGACG
TGGTATTGCAAATAGCTCGCCGCCTGCGCTCTCTGATCGAGAAAGAGGGCAACATGAAGGTGTACATGACGCGCAATGAA
GACATCTTCATTCCGTTGCAGGTACGTGTGGCTAAAGCGCAGAAACAGCGTGCCGACCTGTTTGTCTCTATCCATGCTGA
CGCTTTTACCAGTCGCCAACCGAGCGGTTCCTCGGTATTTGCGCTTTCGACTAAAGGGGCGACCAGTACCGCTGCAAAAT
ATCTGGCACAAACGCAGAACGCCTCGGACTTGATTGGCGGTGTGAGCAAGAGTGGCGACCGCTATGTTGACCACACCATG
TTCGATATGGTGCAGTCACTGACCATTGCCGACAGCCTGAAGTTTGGTAAGGCTGTATTGAATAAGCTGGGTAAAATCAA
CAAACTGCATAAAAATCAGGTTGAACAGGCCGGGTTTGCTGTACTAAAAGCGCCAGATATTCCATCCATTTTGGTGGAAA
CGGCGTTTATCAGTAACGTTGAGGAAGAACGAAAGTTGAAAACCGCAACTTTCCAGCAGGAGATTGCTGAATCTATTCTT
GCAGGGATCAAAGCTTACTTTGCCGATGGTGCGACGCTGGCGAGAAGGGGATGA

Upstream 100 bases:

>100_bases
CTGTTTAATAGATGACAGATTATGCGCTATTCGCTAAAGTTTCCGGTCAATTTGGTCGTTTTGTGTATCTTTACCGTTTA
GATTTTTGCTCGGGAGTAGC

Downstream 100 bases:

>100_bases
TAAAAAAGCGCTGAATGGCGCTTTTTTTATCGAGAGCAGAAACAAAAAAACACCCAACAGGGTGCTTTAGTAATTGGTTG
CGGGGGCCGGATTTGAACCG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSGTNSAISRRRLLQGAGAMWLLSVSQVGLAAVSQVVAVRVWPASSYTRVTVESSRLLKYKQFALSNPERVVVDLEGVNL
NSVLKGVAAQIRTDDPFIKSARVGQFDPQTVRMVFELKQNVKPQIFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQIARRLRSLIEKEGNMKVYMTRNE
DIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTM
FDMVQSLTIADSLKFGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQQEIAESIL
AGIKAYFADGATLARRG

Sequences:

>Translated_417_residues
MSGTNSAISRRRLLQGAGAMWLLSVSQVGLAAVSQVVAVRVWPASSYTRVTVESSRLLKYKQFALSNPERVVVDLEGVNL
NSVLKGVAAQIRTDDPFIKSARVGQFDPQTVRMVFELKQNVKPQIFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQIARRLRSLIEKEGNMKVYMTRNE
DIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTM
FDMVQSLTIADSLKFGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQQEIAESIL
AGIKAYFADGATLARRG
>Mature_416_residues
SGTNSAISRRRLLQGAGAMWLLSVSQVGLAAVSQVVAVRVWPASSYTRVTVESSRLLKYKQFALSNPERVVVDLEGVNLN
SVLKGVAAQIRTDDPFIKSARVGQFDPQTVRMVFELKQNVKPQIFALAPVAGFKERLVMDLYPANAQDMQDPLLALLEDY
NKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQIARRLRSLIEKEGNMKVYMTRNED
IFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMF
DMVQSLTIADSLKFGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQQEIAESILA
GIKAYFADGATLARRG

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=417, Percent_Identity=97.3621103117506, Blast_Score=831, Evalue=0.0,
Organism=Escherichia coli, GI1788776, Length=235, Percent_Identity=48.0851063829787, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1790611, Length=225, Percent_Identity=40.4444444444444, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 45517; Mature: 45386

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTNSAISRRRLLQGAGAMWLLSVSQVGLAAVSQVVAVRVWPASSYTRVTVESSRLLKY
CCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHEEEEECCCCEEEEEECHHHHHHH
KQFALSNPERVVVDLEGVNLNSVLKGVAAQIRTDDPFIKSARVGQFDPQTVRMVFELKQN
HHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHC
VKPQIFALAPVAGFKERLVMDLYPANAQDMQDPLLALLEDYNKGDLEKQVPPAQSGPQPG
CCCEEEEECCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
KAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQIARRLRSLIEKEGNMKVYMTRNE
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQN
CEEEEEEEHHHHHHHHCCCEEEEEECCHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCC
ASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKFGKAVLNKLGKINKLHKNQVEQAGFA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEE
VLKAPDIPSILVETAFISNVEEERKLKTATFQQEIAESILAGIKAYFADGATLARRG
EEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
SGTNSAISRRRLLQGAGAMWLLSVSQVGLAAVSQVVAVRVWPASSYTRVTVESSRLLKY
CCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHEEEEECCCCEEEEEECHHHHHHH
KQFALSNPERVVVDLEGVNLNSVLKGVAAQIRTDDPFIKSARVGQFDPQTVRMVFELKQN
HHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHC
VKPQIFALAPVAGFKERLVMDLYPANAQDMQDPLLALLEDYNKGDLEKQVPPAQSGPQPG
CCCEEEEECCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
KAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQIARRLRSLIEKEGNMKVYMTRNE
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQN
CEEEEEEEHHHHHHHHCCCEEEEEECCHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCC
ASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKFGKAVLNKLGKINKLHKNQVEQAGFA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEE
VLKAPDIPSILVETAFISNVEEERKLKTATFQQEIAESILAGIKAYFADGATLARRG
EEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]