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The map label for this gene is yafL [H]

Identifier: 218547672

GI number: 218547672

Start: 265428

End: 266186

Strand: Direct

Name: yafL [H]

Synonym: EFER_0251

Alternate gene names: 218547672

Gene position: 265428-266186 (Clockwise)

Preceding gene: 218547670

Following gene: 218547674

Centisome position: 5.78

GC content: 52.83

Gene sequence:

>759_bases
ATGTCCTTCATGTCTTCATTCTTTCACGGCAGATTTCTCCATCCAGGCGTGTTTTCGTTATGCGTTTTGCTCCCTTTACT
CGCCAGCGCTACCACATCACCTATCTCTCTCAGTTACGCCGCCCGCCAGCGGATGCAAAACCGTGCCAGACTGCTAAAAC
AGTACCAGGTGCATCTAAAAAAACAGGCGAGCTACATTGTGGAAGGCAATGCCGAAAGCAGAAGGGCGCTGCGCCAGCAC
AACCGGGAGCAGGTAAAACAGCATCCAGAATGGTTTCCTGCACCGCTCAAGGCGAGTGACAGACGCTGGCAGGTGCTGGC
AGAAAACAACCACTTTTTAAGCAGCGACCATCTGCATAACATCACCGAAGTAGCGATTCACCGTCTGGAGCAGCAGCTTG
GCAAGCCTTACGTCTGGGGCGGCACGCGGCCTGATCAAGGCTTCGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAG
ATCCTCGAGGCGAAGCTCCCGCGTACGGCCAATGAGATGTATCACTACCATCGGGCAACGATTGTGGCGAACAACGACCT
GCGCCGGGGAGATTTGCTGTTTTTCCATATTCACAGCCGTGAGATTGCCGATCATATAGGCGTGTATTTGGGAGACGGTC
AGTTTATCGAGTCGCCGCGAACGGGGGAAACCATTCGGGTAAGCCGGTTAGCTGAACCTTTCTGGCAGGATCATTTTTTG
GGGGCTAGACGGATTTTGACTGAAGAAACGATCCTGTAG

Upstream 100 bases:

>100_bases
CTAAGATTATGAAACCTCGTAAGTAATATGCAAATAAGCAATTAATAATCCCGTTTACTCCCCCAACCTTTTAAAATTCT
CTCCTTCATTCAGCTGGTTT

Downstream 100 bases:

>100_bases
CCAGCAGAGCGTGTGTGACACTCGAAGCGCCGGATATCATTCATATCCGGCACCAAACCGGACTAGCCCAAATCCGCCAT
TAACACTTTGGAACGACGCT

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSFMSSFFHGRFLHPGVFSLCVLLPLLASATTSPISLSYAARQRMQNRARLLKQYQVHLKKQASYIVEGNAESRRALRQH
NREQVKQHPEWFPAPLKASDRRWQVLAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNK
ILEAKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHIGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFL
GARRILTEETIL

Sequences:

>Translated_252_residues
MSFMSSFFHGRFLHPGVFSLCVLLPLLASATTSPISLSYAARQRMQNRARLLKQYQVHLKKQASYIVEGNAESRRALRQH
NREQVKQHPEWFPAPLKASDRRWQVLAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNK
ILEAKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHIGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFL
GARRILTEETIL
>Mature_251_residues
SFMSSFFHGRFLHPGVFSLCVLLPLLASATTSPISLSYAARQRMQNRARLLKQYQVHLKKQASYIVEGNAESRRALRQHN
REQVKQHPEWFPAPLKASDRRWQVLAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKI
LEAKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHIGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFLG
ARRILTEETIL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1786421, Length=241, Percent_Identity=90.4564315352697, Blast_Score=459, Evalue=1e-131,
Organism=Escherichia coli, GI1787944, Length=134, Percent_Identity=51.4925373134328, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI1788501, Length=114, Percent_Identity=32.4561403508772, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 29215; Mature: 29083

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFMSSFFHGRFLHPGVFSLCVLLPLLASATTSPISLSYAARQRMQNRARLLKQYQVHLK
CCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KQASYIVEGNAESRRALRQHNREQVKQHPEWFPAPLKASDRRWQVLAENNHFLSSDHLHN
HHCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEECCCHHHH
ITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYHRAT
HHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHEE
IVANNDLRRGDLLFFHIHSREIADHIGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFL
EEECCCCCCCCEEEEEECCHHHHHHCCEEECCCCEECCCCCCCEEEHHHHHHHHHHHHHH
GARRILTEETIL
HHHHHHHHHCCC
>Mature Secondary Structure 
SFMSSFFHGRFLHPGVFSLCVLLPLLASATTSPISLSYAARQRMQNRARLLKQYQVHLK
CHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KQASYIVEGNAESRRALRQHNREQVKQHPEWFPAPLKASDRRWQVLAENNHFLSSDHLHN
HHCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEECCCHHHH
ITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYHRAT
HHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHEE
IVANNDLRRGDLLFFHIHSREIADHIGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFL
EEECCCCCCCCEEEEEECCHHHHHHCCEEECCCCEECCCCCCCEEEHHHHHHHHHHHHHH
GARRILTEETIL
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]