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The map label for this gene is yafC [H]

Identifier: 218547658

GI number: 218547658

Start: 251391

End: 252305

Strand: Reverse

Name: yafC [H]

Synonym: EFER_0237

Alternate gene names: 218547658

Gene position: 252305-251391 (Counterclockwise)

Preceding gene: 218547661

Following gene: 218547655

Centisome position: 5.5

GC content: 48.52

Gene sequence:

>915_bases
ATGAAAGCTACGTCGGAAGAACTCGCCATTTTTGTTTCGGTCGTCGAAAGCGGCAGCTTTAGCCGGGCAGCGGAACAATT
AGGGCAAGCAAACTCAGCGGTAAGCCGGGCGGTGAAAAAGCTGGAGATGAAACTTGGTGTCAGCCTGCTTAATCGGACCA
CGCGGCAACTTAGCCTGACGGAAGAAGGCGAGCGTTATTTTCGTCGCGTACAGTCAATTTTGCAGGAGATGGCAGCGGCA
GAATCAGAAATTATGGAGACGCGTAATACGCCGCGTGGACTGTTACGGATCGATGCTGCAACCCCTGTGGTGCTGCACTT
CCTGATGCCGTTGATTAAACCTTTCCGTGAACGCTATCCGGAAGTCACTTTATCGCTAGTCTCCTCCGAAACGATTATTA
ATTTGATTGAACGAAAAGTGGATGTCGCGATTCGCGCTGGTACGTTAACAGATTCCAGCTTACGAGCCAGGCCATTGTTT
AACAGTTATCGAAAAATTATCGCCTCCCCTGATTATATTGCCCGCTACGGCAAGCCAGAAACTATTGACGATTTAAAGCA
ACATGTTTGCCTGGGATTCACTGAACCCGCTTCTCTCAATACCTGGCCGATAACCTGTAGCGATGGACAATTACATGAGG
TGAAGTACGTTTTGTCATCCAATAGTGGGGAAACACTGAAACAGCTTTGCCTGAGTGGCAACGGGATTGCGTGTTTGTCC
GACTATATGATCGACAGAGAAATTGCTCGTGGTGAACTGGTAGAGTTAATGGCAGATAAAGTACTGCCAGTGGAAATGCC
ATTCAGTGCCGTCTATTACAGCGACCGTGCGGTAAGTACGCGCATCCGGGCTTTTATCGATTTCCTTAGCGAGCATGTAA
AAACAGCTCCCAAAGGAGCTGTTAGAGAGGCTTAA

Upstream 100 bases:

>100_bases
CATTGTTGAGAACACTAAATCGGATGACATATCTCGCGTCCTGATGGTTTAATGACAAATAAATTCAAAATATCTCTTCA
ATTTTGCAACAGGGGCAAAA

Downstream 100 bases:

>100_bases
TCCCATTCAGGAGCCAGACCTTCCGGGCTTACCAGGCGGTCGTTGCAATCCAGTGCGGCGATCGCTTTTTTATCTTCGGC
ATCAAACTGTAAATTTTGTG

Product: DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYIARYGKPETIDDLKQHVCLGFTEPASLNTWPITCSDGQLHEVKYVLSSNSGETLKQLCLSGNGIACLS
DYMIDREIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPKGAVREA

Sequences:

>Translated_304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYIARYGKPETIDDLKQHVCLGFTEPASLNTWPITCSDGQLHEVKYVLSSNSGETLKQLCLSGNGIACLS
DYMIDREIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPKGAVREA
>Mature_304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYIARYGKPETIDDLKQHVCLGFTEPASLNTWPITCSDGQLHEVKYVLSSNSGETLKQLCLSGNGIACLS
DYMIDREIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPKGAVREA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=304, Percent_Identity=97.6973684210526, Blast_Score=607, Evalue=1e-175,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=33.9100346020761, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=28.5223367697594, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=29.3515358361775, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789639, Length=291, Percent_Identity=31.2714776632302, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789440, Length=292, Percent_Identity=28.7671232876712, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787589, Length=300, Percent_Identity=28.3333333333333, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789173, Length=285, Percent_Identity=28.4210526315789, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788706, Length=298, Percent_Identity=25.8389261744966, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI157672245, Length=171, Percent_Identity=32.7485380116959, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788481, Length=292, Percent_Identity=24.3150684931507, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI87082132, Length=313, Percent_Identity=27.1565495207668, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786448, Length=259, Percent_Identity=27.027027027027, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI145693105, Length=165, Percent_Identity=29.0909090909091, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788748, Length=126, Percent_Identity=34.1269841269841, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786508, Length=81, Percent_Identity=40.7407407407407, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787530, Length=268, Percent_Identity=26.1194029850746, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=170, Percent_Identity=25.2941176470588, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789506, Length=174, Percent_Identity=27.5862068965517, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787806, Length=169, Percent_Identity=25.4437869822485, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33864; Mature: 33864

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
EVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLFNSYRKIIASPDYIARYGKPE
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCC
TIDDLKQHVCLGFTEPASLNTWPITCSDGQLHEVKYVLSSNSGETLKQLCLSGNGIACLS
CHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHCCCCHHHHH
DYMIDREIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPKGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCHHHHHHHHHHHHHHHHHHHCCCCCC
VREA
CCCC
>Mature Secondary Structure
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
EVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLFNSYRKIIASPDYIARYGKPE
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCC
TIDDLKQHVCLGFTEPASLNTWPITCSDGQLHEVKYVLSSNSGETLKQLCLSGNGIACLS
CHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHCCCCHHHHH
DYMIDREIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPKGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCHHHHHHHHHHHHHHHHHHHCCCCCC
VREA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]