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The map label for this gene is nlpE [H]

Identifier: 218547648

GI number: 218547648

Start: 236733

End: 237443

Strand: Direct

Name: nlpE [H]

Synonym: EFER_0216

Alternate gene names: 218547648

Gene position: 236733-237443 (Clockwise)

Preceding gene: 218547647

Following gene: 218547656

Centisome position: 5.16

GC content: 52.18

Gene sequence:

>711_bases
ATGGTGAAAAAAGCGATAGTGACAGCGATGGCTGTAATCAGCCTCTTTACACTGATGGGATGTAATAATCGGGCCGAAGT
CGATACGCTTTCCCCGGCGCAGGCCGCTGAACTGAAGCCGATGCCGCAAAGCTGGCGCGGCGTACTGCCGTGTGCTGATT
GCGAAGGTATCGAAACCTCTCTGTTCCTCGAAAAAGATGGAACCTGGGTGATGAATGAGCGTTATCTGGGGGCTCGTGAA
GAACCTTCCTCCTTCGCTTCCTACGGTACATGGGCGCGAACCGCTGACAAACTGGTATTAACCGACAGTAAAGGTGAAAA
GTCATATTATCGGGCGAAAGGAGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATCGAATCGCAGTTCAACTATA
CACTGGAACCGGCAAAGTCCAGCCTGCCAGTGACGCCGATGACTCTACGCGGCATGTATTTTTATATGGCTGATGCGGCA
ACCTTTACTGATTGTGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCTGAGCTGGAACGGGGCTATTTGGCTGCCCG
CGGCAACAGCGAGAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGCTAATCCGGATACTGGTGCGCCGA
CCAAAGTATTTGCGCCCGATACGGCAGGTAAATTTTACCCGCACCAGGATTGCAGTAGTTTGGGGCAGTAA

Upstream 100 bases:

>100_bases
GCATCAAAAGAGCGCAGGCTGGTATCGAAAGCACAAAAATCAAGCGTGAAGGCGTTGCGCGGCAAAGTGCGCGGCGGTCG
GGAATAAAAAGAAGGAATGG

Downstream 100 bases:

>100_bases
GCCATCGTGAGACAGAAACAAACGAAAAATTGCCTGATGCGCAGCGCTTATCAGGCCTACAGCCAATAATGCAACATTTT
GTATTAGCACGATTTTGTAG

Product: lipoprotein

Products: NA

Alternate protein names: Copper homeostasis protein CutF [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYLGARE
EPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAKSSLPVTPMTLRGMYFYMADAA
TFTDCATGKRFMVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVFAPDTAGKFYPHQDCSSLGQ

Sequences:

>Translated_236_residues
MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYLGARE
EPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAKSSLPVTPMTLRGMYFYMADAA
TFTDCATGKRFMVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVFAPDTAGKFYPHQDCSSLGQ
>Mature_236_residues
MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETSLFLEKDGTWVMNERYLGARE
EPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAKSSLPVTPMTLRGMYFYMADAA
TFTDCATGKRFMVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVFAPDTAGKFYPHQDCSSLGQ

Specific function: Involved in copper homeostasis. Could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes. When overproduced induces degP through the activation of the two-component system CpxA/CpxR [H]

COG id: COG3015

COG function: function code MP; Uncharacterized lipoprotein NlpE involved in copper resistance

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786390, Length=236, Percent_Identity=96.6101694915254, Blast_Score=476, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007298 [H]

Pfam domain/function: PF04170 NlpE [H]

EC number: NA

Molecular weight: Translated: 25857; Mature: 25857

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00283 SOYBEAN_KUNITZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHHCCCCCCCCCCCCCEE
LFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEML
EEEECCCCEEECCCCCCCCCCCHHHHHCCCHHHCCCEEEEECCCCCCHHHHCCCHHHHHH
DREGNPIESQFNYTLEPAKSSLPVTPMTLRGMYFYMADAATFTDCATGKRFMVANNAELE
HCCCCCCCCCCCEEECCCCCCCCCCCHHHCCEEEEEECCCHHHHHCCCCEEEEECCCCCC
RGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVFAPDTAGKFYPHQDCSSLGQ
CCEEEECCCCCCCEEEEECCEEEEECCCCCCCCCEEECCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQSWRGVLPCADCEGIETS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHHCCCCCCCCCCCCCEE
LFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTDSKGEKSYYRAKGDALEML
EEEECCCCEEECCCCCCCCCCCHHHHHCCCHHHCCCEEEEECCCCCCHHHHCCCHHHHHH
DREGNPIESQFNYTLEPAKSSLPVTPMTLRGMYFYMADAATFTDCATGKRFMVANNAELE
HCCCCCCCCCCCEEECCCCCCCCCCCHHHCCEEEEEECCCHHHHHCCCCEEEEECCCCCC
RGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVFAPDTAGKFYPHQDCSSLGQ
CCEEEECCCCCCCEEEEECCEEEEECCCCCCCCCEEECCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7635808; 7635807; 9278503 [H]