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The map label for this gene is yaeI [H]

Identifier: 218547620

GI number: 218547620

Start: 206006

End: 206821

Strand: Reverse

Name: yaeI [H]

Synonym: EFER_0187

Alternate gene names: 218547620

Gene position: 206821-206006 (Counterclockwise)

Preceding gene: 218547621

Following gene: 218547619

Centisome position: 4.51

GC content: 50.12

Gene sequence:

>816_bases
ATGATGATCACACGCAGGCGTTTTTTACAGGCTGCCGCCGCGACGTTAACGGTGAGTTCAAGTTTCGGTTATATGCATTA
CCTGGAACCCGGCTGGCTTGAATTAACCCATCACCGCTTCGCCTTTTTTGAAGAAAAAGCCGCACCATTCAAAATTCTGT
TTCTGGCCGATCTCCATTATTCCCGCTTTGTTCCGCTTACCTTGATTTCAGACGCCATTAATCTTGGAGTTGCACAAAAG
CCCGATTTGATATTGCTGGGCGGTGATTACGTATTATTCGATATGCCGCTGAATTTTTCGGCGTTTGCTGACGTACTCTC
CCCCCTTGCCGAATGTGCGCCGACGTTTGCCTGTTATGGCAATCACGATCGCCCCGTGGGAACGGTGAAAAATCGCTTGA
TTGGCGAGGCGTTAAAGTCGGCGGGGATCACGGTGTTATTTAACGAATCCACGCTAATCACCACTCAAAAGCAGCAATTT
GAACTGGTTGGCACTGGTGATTTATGGGCTGGACAATGCAAACCGCCTCCCGCCAGCGAAGCAAATCTGCCACGACTGGT
GCTGGCACATAATCCCGACAGCAAAGAAGTCATGCGTAATGAAAAGTGGGATCTGATGCTCTGTGGTCACACTCACGGCG
GGCAGTTACGCATACCGTTGGTTGGTGAGCCTTTTGCGCCCATAGAAGATAAACGCTACATCGCTGGTTTAAACGCCTTT
GGTGAGCGACAAATTTACACGACTCGGGGTGTCGGAAGTTTGTATGGCTTGCGTCTGAATTGCCGCCCGGAAGTGACGAT
CATGGAACTGGTGTAG

Upstream 100 bases:

>100_bases
AAACTCGTGGCAAAGTCGGCATTAACGAACTGCTACGTACCATCGACTAAAAGTCAGTACACGGGCAGCACGACGCTGCC
CGATTTTTTTACAGGGATGA

Downstream 100 bases:

>100_bases
AAAGCGAATTCTCTTATCAGAATAAAGCAGGTAATTTTTTGATTTTTAGACCATGATAAAAGTCTGGAAGAAAAGGCTTC
CTGCGATCTCCGTCGAGCTA

Product: phosphodiesterase YaeI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MMITRRRFLQAAAATLTVSSSFGYMHYLEPGWLELTHHRFAFFEEKAAPFKILFLADLHYSRFVPLTLISDAINLGVAQK
PDLILLGGDYVLFDMPLNFSAFADVLSPLAECAPTFACYGNHDRPVGTVKNRLIGEALKSAGITVLFNESTLITTQKQQF
ELVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRNEKWDLMLCGHTHGGQLRIPLVGEPFAPIEDKRYIAGLNAF
GERQIYTTRGVGSLYGLRLNCRPEVTIMELV

Sequences:

>Translated_271_residues
MMITRRRFLQAAAATLTVSSSFGYMHYLEPGWLELTHHRFAFFEEKAAPFKILFLADLHYSRFVPLTLISDAINLGVAQK
PDLILLGGDYVLFDMPLNFSAFADVLSPLAECAPTFACYGNHDRPVGTVKNRLIGEALKSAGITVLFNESTLITTQKQQF
ELVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRNEKWDLMLCGHTHGGQLRIPLVGEPFAPIEDKRYIAGLNAF
GERQIYTTRGVGSLYGLRLNCRPEVTIMELV
>Mature_271_residues
MMITRRRFLQAAAATLTVSSSFGYMHYLEPGWLELTHHRFAFFEEKAAPFKILFLADLHYSRFVPLTLISDAINLGVAQK
PDLILLGGDYVLFDMPLNFSAFADVLSPLAECAPTFACYGNHDRPVGTVKNRLIGEALKSAGITVLFNESTLITTQKQQF
ELVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRNEKWDLMLCGHTHGGQLRIPLVGEPFAPIEDKRYIAGLNAF
GERQIYTTRGVGSLYGLRLNCRPEVTIMELV

Specific function: Shows phosphodiesterase activity, hydrolyzing phosphodiester bond in the artificial chromogenic substrate bis-p- nitrophenyl phosphate (bis-pNPP) [H]

COG id: COG1408

COG function: function code R; Predicted phosphohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallophosphoesterase superfamily [H]

Homologues:

Organism=Escherichia coli, GI87081695, Length=270, Percent_Identity=85.5555555555556, Blast_Score=493, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI115535030, Length=220, Percent_Identity=29.0909090909091, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115535028, Length=220, Percent_Identity=29.0909090909091, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 30140; Mature: 30140

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMITRRRFLQAAAATLTVSSSFGYMHYLEPGWLELTHHRFAFFEEKAAPFKILFLADLHY
CCCCHHHHHHHHHHEEEEECCCCEEEECCCCCEEEECHHEEEECCCCCCEEEEEEEECCC
SRFVPLTLISDAINLGVAQKPDLILLGGDYVLFDMPLNFSAFADVLSPLAECAPTFACYG
CCEEHHHHHHHHHHCCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEC
NHDRPVGTVKNRLIGEALKSAGITVLFNESTLITTQKQQFELVGTGDLWAGQCKPPPASE
CCCCCHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCEEEEEECCCCCCCCCCCCCCCC
ANLPRLVLAHNPDSKEVMRNEKWDLMLCGHTHGGQLRIPLVGEPFAPIEDKRYIAGLNAF
CCCCEEEEEECCCHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCEEEEECCCC
GERQIYTTRGVGSLYGLRLNCRPEVTIMELV
CCCEEEEECCCCCEEEEEEECCCCEEEEEEC
>Mature Secondary Structure
MMITRRRFLQAAAATLTVSSSFGYMHYLEPGWLELTHHRFAFFEEKAAPFKILFLADLHY
CCCCHHHHHHHHHHEEEEECCCCEEEECCCCCEEEECHHEEEECCCCCCEEEEEEEECCC
SRFVPLTLISDAINLGVAQKPDLILLGGDYVLFDMPLNFSAFADVLSPLAECAPTFACYG
CCEEHHHHHHHHHHCCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEC
NHDRPVGTVKNRLIGEALKSAGITVLFNESTLITTQKQQFELVGTGDLWAGQCKPPPASE
CCCCCHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCEEEEEECCCCCCCCCCCCCCCC
ANLPRLVLAHNPDSKEVMRNEKWDLMLCGHTHGGQLRIPLVGEPFAPIEDKRYIAGLNAF
CCCCEEEEEECCCHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCEEEEECCCC
GERQIYTTRGVGSLYGLRLNCRPEVTIMELV
CCCEEEEECCCCCEEEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503 [H]