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The map label for this gene is fruR [H]

Identifier: 218547537

GI number: 218547537

Start: 110005

End: 111009

Strand: Direct

Name: fruR [H]

Synonym: EFER_0102

Alternate gene names: 218547537

Gene position: 110005-111009 (Clockwise)

Preceding gene: 218547536

Following gene: 218547538

Centisome position: 2.4

GC content: 52.54

Gene sequence:

>1005_bases
GTGAAACTGGATGAAATCGCTCGGCTGGCGGGAGTGTCGCGGACCACTGCAAGCTATGTTATTAACGGCAAAGCGAAGCA
ATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAG
CTGGGCTTCGTGCTGGACGCACACGATCGATCGGGCTGGTAATCCCTGATCTGGAAAACACGAGCTATACCCGTATAGCA
AATTACCTGGAGCGCCAGGCTCGCCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAACCAGACAACGAAAT
GCGCTGTATCGAGCATCTTTTACAGCGTCAGGTAGATGCCATTATTGTTTCCACTTCGTTGCCACCGGAGCATCCGTTCT
ATCAACGTTGGGCTAACGACACCTTCCCGATTGTTGCGCTGGACCGCGCTCTTGATCGTGAGCATTTTACCAGTGTGGTT
GGTGCGGATCAGGACGATGCCGAAATGCTGGCGGAGGAGCTACGTAAATTCCCCGCAGAAACCGTGCTTTATCTTGGTGC
GCTGCCGGAGCTTTCTGTCAGCTTCCTGCGTGAGCAAGGGTTTCGTACTGCGTGGAAAGATGATCCGCGTGAAGTCCATT
TCCTCTACGCCAACAGTTATGAGCGTGAAGCTGCCGGACAGCTGTTTGAGAAATGGCTGGAAACGCACCCAATGCCACAA
GCATTATTCACCACCTCGTTTGCTTTGTTGCAAGGGGTAATGGATGTCACACTGCGTCGTGACGGTAAGTTACCTTCCGA
TCTGGCTATTGCTACTTTTGGTGATAACGAACTGCTGGATTTCCTGCAATGCCCGGTACTGGCGGTGGCGCAGCGTCATC
GTGATGTTGCCGAACGTGTCCTTGAAATCGTGCTGGCGAGCCTTGATGAACCACGTAAACCTAAACCTGGTTTAACGCGC
ATTAAGCGTAATCTCTATCGCCGTGGAGTATTGAGCCGCAGCTAA

Upstream 100 bases:

>100_bases
AAGCGGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGATATGGTTTTTAC
ATTTTACGCAAGGGGCAATT

Downstream 100 bases:

>100_bases
GCGTTTTATGAAAATTGGCGCCGGAGAAAAAACTCTGGCGCTTTTCGGGATAAAATCTTATTTCCTTACCATAATTAAGA
TTATTCCTGAGCTTTGGCAT

Product: DNA-binding transcriptional regulator FruR

Products: NA

Alternate protein names: Catabolite repressor/activator [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIA
NYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDTFPIVALDRALDREHFTSVV
GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAGQLFEKWLETHPMPQ
ALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTR
IKRNLYRRGVLSRS

Sequences:

>Translated_334_residues
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIA
NYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDTFPIVALDRALDREHFTSVV
GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAGQLFEKWLETHPMPQ
ALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTR
IKRNLYRRGVLSRS
>Mature_334_residues
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIA
NYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDTFPIVALDRALDREHFTSVV
GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAGQLFEKWLETHPMPQ
ALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTR
IKRNLYRRGVLSRS

Specific function: Represses some operons but activates others. It is implicated in the regulation of a large number of operons encoding enzymes which comprise central pathways of carbon metabolism. Binds D-fructose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786268, Length=334, Percent_Identity=99.4011976047904, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI1790194, Length=318, Percent_Identity=28.3018867924528, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787580, Length=289, Percent_Identity=23.5294117647059, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=22.2222222222222, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790715, Length=284, Percent_Identity=26.4084507042254, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789068, Length=304, Percent_Identity=25, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790369, Length=312, Percent_Identity=22.7564102564103, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789202, Length=128, Percent_Identity=32.03125, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788474, Length=208, Percent_Identity=26.4423076923077, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012781
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37990; Mature: 37990

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGR
CCHHHHHHHHCCCHHHHHHEECCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
TRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDA
CCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHE
IIVSTSLPPEHPFYQRWANDTFPIVALDRALDREHFTSVVGADQDDAEMLAEELRKFPAE
EEEECCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHH
TVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAGQLFEKWLETHPMPQ
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCH
ALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRDVAERV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGR
CCHHHHHHHHCCCHHHHHHEECCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
TRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDA
CCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHE
IIVSTSLPPEHPFYQRWANDTFPIVALDRALDREHFTSVVGADQDDAEMLAEELRKFPAE
EEEECCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHH
TVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAGQLFEKWLETHPMPQ
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCH
ALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRDVAERV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]