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The map label for this gene is citA [H]

Identifier: 218547466

GI number: 218547466

Start: 25391

End: 27019

Strand: Reverse

Name: citA [H]

Synonym: EFER_0027

Alternate gene names: 218547466

Gene position: 27019-25391 (Counterclockwise)

Preceding gene: 218547467

Following gene: 218547465

Centisome position: 0.59

GC content: 41.87

Gene sequence:

>1629_bases
GTGATAATTCAAATCGTGATTCAGTGGTTCTCCCGACGCTCATTTCAAAATCGCATCTTCTTGCTGATTCTTTTCACTTC
TACTGTCGTGATGCTGGCGATTTCCTGGTATTTAACCAATATCACTAAAGAGCGTCTGCACTATCAGGTAGGTCAACGGG
CCTTGATCCAGGCGATGCAAATCTCCGCCATGCCAGAACTGGTTGAAGCCGTCGAAAAAAATGATCTGGCCCGAATTAAA
GCGCTAATAGATCCAATGCATTCATTTTCCGATGCCACCTACATTACCGTTGGCGATGCCAAAGGTATTCGACTTTATCA
TGTAGTGCCAAATGAAATTGGTCAGCAAATGGAAGGTGGCGATAACGACGAAGCGCTGATTAATGCCAAAAGTTATGTTT
CTGTACGGCAAGGTTCGCTGGGCTCTTCGTTACGGGGAAAATCGCCGATTCAGGACGCGACAGGTAAAGTGATAGGGATT
GTATCGGTGGGATATACCCTGCAACAACTGGAAAGCTGGTTGAGTCTGCAACATAGTTCGTTAATGTTACCCATGCTTGC
CCTACTTTTGTTGTTGTTATTTTCTGCTCGGTTGTTTTCATTACATATTAAACGGCAGATGTTTAATATGGAGCCACAAC
AAATATCGAAACTGGTATTACAACAAAATGTGTTATTTGAGTCAGTATTTGAAGGTTTAATTGCCATTGATTCACAACAT
CAGATCACTGCAATTAATCAGACGGCCCGACAGATGCTTAATCAGCCATATCCTGAGCAATATTTAATTGGCAAAAAAAT
ATCCGATGTTGTTTCGCCAGATACCTTTTTTTACGATAAGCCGCAGAATAATAAAAAAGATGAGATAGTTACCTTTAATC
AAATTAAAGTTATTGCCAGCCGTATGGCCGTCATTCTCGATGAAAAACCGCAAGGTTGGGTCATTAGCTTTCGAAGTAAA
GATGATATTAATACTCTCAGCCTGCAACTGAGTCAGATTCAACAATATGCCGATAATTTACGTGCAGTTCAGCACGAGCA
TCGTAACGTGATTTCTACTATTGCTGGCTTGCTTTATTTAAAACGCTACGACCATGCTCTTAACCTGATTCAACAACAGT
CTGACAGCCACCAAAAAGTGATTGATTTTATTGCACAAAAATTCAAAAACAGTCACCTTGCTGGCCTGTTGATCGGTAAA
TACTATCGCGCCAAAGAGTTGGATCTTGAATTGGTGTTCGATCCCGCCTGCTATGTAGATTCACTACCCACATCGCTCAC
CCATAATGAGTGGATTTCCATTGTTGGTAATCTGCTGGATAACGCCTTTAATGCCACGCTGAAACAGCCTGATGGTAGCC
GTAAAATTGAATGTCTGATTAACAGCGAGGGCGATGAAATACTGATTGAAGTAGCCGATCAGGGCTGTGGTATTGACGAT
AGTATTCGCGATCATATTTTTGAACGTGGTATTACCAGCAGTAATAACGCTGAACACGGTATTGGTTTGTGGCTGGTTAA
AAGCTACACCGAACAGGCGGGTGGGCATATCGTTATTGATGACAACATCCCCTATGGCACCATATTTAGTTTATATATTC
CCCTGACCAGAGAACAACATCATGGATAA

Upstream 100 bases:

>100_bases
TTCCTTTTTAAACGTTTTCCGAATTTCATTGAAATATGCGCCAGGTCGGCCCGTAACCGCAATGAAAAGGCGAAAATCAT
ATCCGTAAATTATTTCTCAG

Downstream 100 bases:

>100_bases
GATAACCACACTGATAGTTGAGGATGAGCCATTACTGGCAGAGATTTTGGTTGATACACTTAAGCAACTCCCGGCTTTTG
ATGTTATTGGCGTTGCGGAT

Product: sensory histidine kinase in two-component regulatory system with citB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MIIQIVIQWFSRRSFQNRIFLLILFTSTVVMLAISWYLTNITKERLHYQVGQRALIQAMQISAMPELVEAVEKNDLARIK
ALIDPMHSFSDATYITVGDAKGIRLYHVVPNEIGQQMEGGDNDEALINAKSYVSVRQGSLGSSLRGKSPIQDATGKVIGI
VSVGYTLQQLESWLSLQHSSLMLPMLALLLLLLFSARLFSLHIKRQMFNMEPQQISKLVLQQNVLFESVFEGLIAIDSQH
QITAINQTARQMLNQPYPEQYLIGKKISDVVSPDTFFYDKPQNNKKDEIVTFNQIKVIASRMAVILDEKPQGWVISFRSK
DDINTLSLQLSQIQQYADNLRAVQHEHRNVISTIAGLLYLKRYDHALNLIQQQSDSHQKVIDFIAQKFKNSHLAGLLIGK
YYRAKELDLELVFDPACYVDSLPTSLTHNEWISIVGNLLDNAFNATLKQPDGSRKIECLINSEGDEILIEVADQGCGIDD
SIRDHIFERGITSSNNAEHGIGLWLVKSYTEQAGGHIVIDDNIPYGTIFSLYIPLTREQHHG

Sequences:

>Translated_542_residues
MIIQIVIQWFSRRSFQNRIFLLILFTSTVVMLAISWYLTNITKERLHYQVGQRALIQAMQISAMPELVEAVEKNDLARIK
ALIDPMHSFSDATYITVGDAKGIRLYHVVPNEIGQQMEGGDNDEALINAKSYVSVRQGSLGSSLRGKSPIQDATGKVIGI
VSVGYTLQQLESWLSLQHSSLMLPMLALLLLLLFSARLFSLHIKRQMFNMEPQQISKLVLQQNVLFESVFEGLIAIDSQH
QITAINQTARQMLNQPYPEQYLIGKKISDVVSPDTFFYDKPQNNKKDEIVTFNQIKVIASRMAVILDEKPQGWVISFRSK
DDINTLSLQLSQIQQYADNLRAVQHEHRNVISTIAGLLYLKRYDHALNLIQQQSDSHQKVIDFIAQKFKNSHLAGLLIGK
YYRAKELDLELVFDPACYVDSLPTSLTHNEWISIVGNLLDNAFNATLKQPDGSRKIECLINSEGDEILIEVADQGCGIDD
SIRDHIFERGITSSNNAEHGIGLWLVKSYTEQAGGHIVIDDNIPYGTIFSLYIPLTREQHHG
>Mature_542_residues
MIIQIVIQWFSRRSFQNRIFLLILFTSTVVMLAISWYLTNITKERLHYQVGQRALIQAMQISAMPELVEAVEKNDLARIK
ALIDPMHSFSDATYITVGDAKGIRLYHVVPNEIGQQMEGGDNDEALINAKSYVSVRQGSLGSSLRGKSPIQDATGKVIGI
VSVGYTLQQLESWLSLQHSSLMLPMLALLLLLLFSARLFSLHIKRQMFNMEPQQISKLVLQQNVLFESVFEGLIAIDSQH
QITAINQTARQMLNQPYPEQYLIGKKISDVVSPDTFFYDKPQNNKKDEIVTFNQIKVIASRMAVILDEKPQGWVISFRSK
DDINTLSLQLSQIQQYADNLRAVQHEHRNVISTIAGLLYLKRYDHALNLIQQQSDSHQKVIDFIAQKFKNSHLAGLLIGK
YYRAKELDLELVFDPACYVDSLPTSLTHNEWISIVGNLLDNAFNATLKQPDGSRKIECLINSEGDEILIEVADQGCGIDD
SIRDHIFERGITSSNNAEHGIGLWLVKSYTEQAGGHIVIDDNIPYGTIFSLYIPLTREQHHG

Specific function: Member of the two-component regulatory system CitA/CitB. Probably activates CitB by phosphorylation. The periplasmic domain binds H-citrate(2-), which is essential for induction of the citrate-fermentation genes [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=532, Percent_Identity=42.2932330827068, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1790567, Length=531, Percent_Identity=28.4369114877589, Blast_Score=193, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61293; Mature: 61293

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIQIVIQWFSRRSFQNRIFLLILFTSTVVMLAISWYLTNITKERLHYQVGQRALIQAMQ
CHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISAMPELVEAVEKNDLARIKALIDPMHSFSDATYITVGDAKGIRLYHVVPNEIGQQMEGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCHHHHHHCCCC
DNDEALINAKSYVSVRQGSLGSSLRGKSPIQDATGKVIGIVSVGYTLQQLESWLSLQHSS
CCCCEEEEHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
LMLPMLALLLLLLFSARLFSLHIKRQMFNMEPQQISKLVLQQNVLFESVFEGLIAIDSQH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCC
QITAINQTARQMLNQPYPEQYLIGKKISDVVSPDTFFYDKPQNNKKDEIVTFNQIKVIAS
HHHHHHHHHHHHHCCCCCCHHHHCCHHHHHCCCCCEEECCCCCCCCCCEEEHHHHHHHHH
RMAVILDEKPQGWVISFRSKDDINTLSLQLSQIQQYADNLRAVQHEHRNVISTIAGLLYL
HHHHEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRYDHALNLIQQQSDSHQKVIDFIAQKFKNSHLAGLLIGKYYRAKELDLELVFDPACYVD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHC
SLPTSLTHNEWISIVGNLLDNAFNATLKQPDGSRKIECLINSEGDEILIEVADQGCGIDD
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCH
SIRDHIFERGITSSNNAEHGIGLWLVKSYTEQAGGHIVIDDNIPYGTIFSLYIPLTREQH
HHHHHHHHCCCCCCCCCCCCCEEHEEHHHHHHCCCEEEEECCCCCCEEEEEEEECCCCCC
HG
CC
>Mature Secondary Structure
MIIQIVIQWFSRRSFQNRIFLLILFTSTVVMLAISWYLTNITKERLHYQVGQRALIQAMQ
CHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISAMPELVEAVEKNDLARIKALIDPMHSFSDATYITVGDAKGIRLYHVVPNEIGQQMEGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCHHHHHHCCCC
DNDEALINAKSYVSVRQGSLGSSLRGKSPIQDATGKVIGIVSVGYTLQQLESWLSLQHSS
CCCCEEEEHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
LMLPMLALLLLLLFSARLFSLHIKRQMFNMEPQQISKLVLQQNVLFESVFEGLIAIDSQH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCC
QITAINQTARQMLNQPYPEQYLIGKKISDVVSPDTFFYDKPQNNKKDEIVTFNQIKVIAS
HHHHHHHHHHHHHCCCCCCHHHHCCHHHHHCCCCCEEECCCCCCCCCCEEEHHHHHHHHH
RMAVILDEKPQGWVISFRSKDDINTLSLQLSQIQQYADNLRAVQHEHRNVISTIAGLLYL
HHHHEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRYDHALNLIQQQSDSHQKVIDFIAQKFKNSHLAGLLIGKYYRAKELDLELVFDPACYVD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHC
SLPTSLTHNEWISIVGNLLDNAFNATLKQPDGSRKIECLINSEGDEILIEVADQGCGIDD
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCH
SIRDHIFERGITSSNNAEHGIGLWLVKSYTEQAGGHIVIDDNIPYGTIFSLYIPLTREQH
HHHHHHHHCCCCCCCCCCCCCEEHEEHHHHHHCCCEEEEECCCCCCEEEEEEEECCCCCC
HG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8748036; 10447894 [H]