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The map label for this gene is proS2 [H]

Identifier: 218235963

GI number: 218235963

Start: 3838533

End: 3840233

Strand: Reverse

Name: proS2 [H]

Synonym: BCB4264_A3917

Alternate gene names: 218235963

Gene position: 3840233-3838533 (Counterclockwise)

Preceding gene: 218234918

Following gene: 218234060

Centisome position: 70.87

GC content: 38.1

Gene sequence:

>1701_bases
ATGAAACAAAGTATGGTATTCAGTCCTACATTACGTGAAGTTCCAGCTGATGCTGAAATTAAGAGTCATCAGCTATTACT
TCGTGCAGGTTTTATGCGTCAAAATGCTTCTGGTATTTATAGCTTTCTACCATTTGGATTAAAAGTATTACACAAAGTAG
AACGTATCGTTCGAGAAGAAATGGAGCGCGCAGGAGCTGTAGAATTATTAATGCCAGCTATGCAAGCTGCGGAATTGTGG
CAAGAATCAGGTCGTTGGTATTCTTACGGATCTGAATTAATGCGTATGAAAGATCGTAATGCTCGTGAATTCGCATTAGG
AGCGACACATGAGGAAGTAATTACGGATCTTGTGCGTGATGAAGTGAAATCATATAAAAAATTACCGTTAACATTATACC
AAATTCAAACAAAATTCCGTGATGAGCAAAGACCTCGTTTCGGCTTATTACGTGGAAGAGAGTTTCTAATGAAAGATGCA
TACTCTTTCCATGCTACACAAGAGAGCTTAGATGAAGTATACGATCGTTTATATAAAGCATACTCTAACATTTTTGCTCG
CTGTGGCTTGAATTTCCGTGCAGTTATTGCTGATTCTGGAGCAATGGGCGGAAAAGATACACATGAATTTATGGTATTAT
CTGATGTTGGTGAAGATACAATTGCATACTCTGACACATCTGATTATGCAGCAAACATCGAAATGGCTCCTGTTGTAGCT
ACGTATACGAAGAGTGACGAAGCAGAAAAAGCACTTGAAAAAGTAGCAACACCAGACCAAAAAGCAATTGAAGAAGTATC
TGCATTCTTAAACATTGCAGCTGACAAGTGCATTAAGTCTATGGTGTTTAAAGTAGATGAGAAATTAGTAGTGGTACTTG
TTCGTGGTGATCACGAAGTAAACGATGTGAAAGTGAAAAATGTATACGGCGCTTCAGTTGTTGAGCTTGCTTCTCATGAA
GAAGTAAAAGCATTATTAAATTGTGAAGTTGGTTCATTAGGACCGATTAATGTAACAGGTGATATCGAAATTATTGCTGA
TCATGCTGTAGCATCAATTGTTAACGGATGTTCAGGAGCGAATGAAGAAGGATTCCATTATGTAAATGTAAATCCAGAAC
GTGACTTTAAAGTAAGTCAATATACAGATTTACGCTTCATCCAAGAAGGAGACCAATCTCCAGATGGAAACGGAACAATT
CTTTTTGCACGCGGAATTGAAGTTGGTCATGTATTCAAATTAGGAACTCGTTATAGTGAAGCAATGAACGCAACATTCCT
AGATGAAAACGGAAAAACACAACCACTTATTATGGGTTGTTACGGTATCGGTGTATCTCGTACAGTGGCAGCAATTGCAG
AGCAGTTTAACGATGAGAACGGCTTAGTTTGGCCAAAAGCTGTAGCACCGTTCCATGTGCATGTAATTCCAGTAAATATG
AAATCGGATGCACAACGTGAAATGGGTGAAAATATCTACAACTCATTACAAGAACAAGGCTATGAAGTGTTATTAGACGA
TCGTGCAGAACGTGCAGGTGTTAAATTTGCAGATGCAGATTTATTCGGTCTTCCAGTTCGTGTTACAGTTGGTAAAAAAG
CAGACGAAGGTATTGTAGAAGTGAAAGTACGTGCTACAGGTGAGTCTGAAGAAGTAAACGTAGAAGAACTTCAAACATAT
ATTGCAAATATTTTAAAATAA

Upstream 100 bases:

>100_bases
AAACTTATAATGAAATAGAGCTCGTAGAATAAAATCCCTCACCTAACTCGAGGTGAGGGGTTTTACTGCGTGTAAGAGTA
AAATGAAGAGGTGCAAATAA

Downstream 100 bases:

>100_bases
GAAGAGGTACCTCGTAATGGGGTACCTTCTCTTTCTTTGTAAACGTATTTTGTAAAAGAAGAGGTGAGAAATCTTTCTTT
GTGGTACGTTTCAATTTATA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase 1; ProRS 1 [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPFGLKVLHKVERIVREEMERAGAVELLMPAMQAAELW
QESGRWYSYGSELMRMKDRNAREFALGATHEEVITDLVRDEVKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDA
YSFHATQESLDEVYDRLYKAYSNIFARCGLNFRAVIADSGAMGGKDTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVVA
TYTKSDEAEKALEKVATPDQKAIEEVSAFLNIAADKCIKSMVFKVDEKLVVVLVRGDHEVNDVKVKNVYGASVVELASHE
EVKALLNCEVGSLGPINVTGDIEIIADHAVASIVNGCSGANEEGFHYVNVNPERDFKVSQYTDLRFIQEGDQSPDGNGTI
LFARGIEVGHVFKLGTRYSEAMNATFLDENGKTQPLIMGCYGIGVSRTVAAIAEQFNDENGLVWPKAVAPFHVHVIPVNM
KSDAQREMGENIYNSLQEQGYEVLLDDRAERAGVKFADADLFGLPVRVTVGKKADEGIVEVKVRATGESEEVNVEELQTY
IANILK

Sequences:

>Translated_566_residues
MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPFGLKVLHKVERIVREEMERAGAVELLMPAMQAAELW
QESGRWYSYGSELMRMKDRNAREFALGATHEEVITDLVRDEVKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDA
YSFHATQESLDEVYDRLYKAYSNIFARCGLNFRAVIADSGAMGGKDTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVVA
TYTKSDEAEKALEKVATPDQKAIEEVSAFLNIAADKCIKSMVFKVDEKLVVVLVRGDHEVNDVKVKNVYGASVVELASHE
EVKALLNCEVGSLGPINVTGDIEIIADHAVASIVNGCSGANEEGFHYVNVNPERDFKVSQYTDLRFIQEGDQSPDGNGTI
LFARGIEVGHVFKLGTRYSEAMNATFLDENGKTQPLIMGCYGIGVSRTVAAIAEQFNDENGLVWPKAVAPFHVHVIPVNM
KSDAQREMGENIYNSLQEQGYEVLLDDRAERAGVKFADADLFGLPVRVTVGKKADEGIVEVKVRATGESEEVNVEELQTY
IANILK
>Mature_566_residues
MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPFGLKVLHKVERIVREEMERAGAVELLMPAMQAAELW
QESGRWYSYGSELMRMKDRNAREFALGATHEEVITDLVRDEVKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDA
YSFHATQESLDEVYDRLYKAYSNIFARCGLNFRAVIADSGAMGGKDTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVVA
TYTKSDEAEKALEKVATPDQKAIEEVSAFLNIAADKCIKSMVFKVDEKLVVVLVRGDHEVNDVKVKNVYGASVVELASHE
EVKALLNCEVGSLGPINVTGDIEIIADHAVASIVNGCSGANEEGFHYVNVNPERDFKVSQYTDLRFIQEGDQSPDGNGTI
LFARGIEVGHVFKLGTRYSEAMNATFLDENGKTQPLIMGCYGIGVSRTVAAIAEQFNDENGLVWPKAVAPFHVHVIPVNM
KSDAQREMGENIYNSLQEQGYEVLLDDRAERAGVKFADADLFGLPVRVTVGKKADEGIVEVKVRATGESEEVNVEELQTY
IANILK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=42.9203539823009, Blast_Score=213, Evalue=4e-55,
Organism=Escherichia coli, GI1786392, Length=573, Percent_Identity=51.4834205933682, Blast_Score=599, Evalue=1e-172,
Organism=Caenorhabditis elegans, GI115532348, Length=241, Percent_Identity=36.0995850622407, Blast_Score=161, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI193203271, Length=85, Percent_Identity=36.4705882352941, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=226, Percent_Identity=38.9380530973451, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24656200, Length=256, Percent_Identity=33.59375, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63035; Mature: 63035

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPFGLKVLHKVERIVREE
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MERAGAVELLMPAMQAAELWQESGRWYSYGSELMRMKDRNAREFALGATHEEVITDLVRD
HHHCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHH
EVKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDAYSFHATQESLDEVYDRLYKA
HHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSNIFARCGLNFRAVIADSGAMGGKDTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVVA
HHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEECCEEE
TYTKSDEAEKALEKVATPDQKAIEEVSAFLNIAADKCIKSMVFKVDEKLVVVLVRGDHEV
EECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
NDVKVKNVYGASVVELASHEEVKALLNCEVGSLGPINVTGDIEIIADHAVASIVNGCSGA
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEHHHHHHHHHCCCCC
NEEGFHYVNVNPERDFKVSQYTDLRFIQEGDQSPDGNGTILFARGIEVGHVFKLGTRYSE
CCCCEEEEECCCCCCCEECCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEECCCHHHH
AMNATFLDENGKTQPLIMGCYGIGVSRTVAAIAEQFNDENGLVWPKAVAPFHVHVIPVNM
HCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEECC
KSDAQREMGENIYNSLQEQGYEVLLDDRAERAGVKFADADLFGLPVRVTVGKKADEGIVE
CCHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCEEECCCEECCEEEEEECCCCCCCEEE
VKVRATGESEEVNVEELQTYIANILK
EEEEECCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPFGLKVLHKVERIVREE
CCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MERAGAVELLMPAMQAAELWQESGRWYSYGSELMRMKDRNAREFALGATHEEVITDLVRD
HHHCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHH
EVKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDAYSFHATQESLDEVYDRLYKA
HHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSNIFARCGLNFRAVIADSGAMGGKDTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVVA
HHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEECCEEE
TYTKSDEAEKALEKVATPDQKAIEEVSAFLNIAADKCIKSMVFKVDEKLVVVLVRGDHEV
EECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
NDVKVKNVYGASVVELASHEEVKALLNCEVGSLGPINVTGDIEIIADHAVASIVNGCSGA
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEHHHHHHHHHCCCCC
NEEGFHYVNVNPERDFKVSQYTDLRFIQEGDQSPDGNGTILFARGIEVGHVFKLGTRYSE
CCCCEEEEECCCCCCCEECCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEECCCHHHH
AMNATFLDENGKTQPLIMGCYGIGVSRTVAAIAEQFNDENGLVWPKAVAPFHVHVIPVNM
HCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEECC
KSDAQREMGENIYNSLQEQGYEVLLDDRAERAGVKFADADLFGLPVRVTVGKKADEGIVE
CCHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCEEECCCEECCEEEEEECCCCCCCEEE
VKVRATGESEEVNVEELQTYIANILK
EEEEECCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA