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The map label for this gene is metS [H]

Identifier: 218235634

GI number: 218235634

Start: 41506

End: 43488

Strand: Direct

Name: metS [H]

Synonym: BCB4264_A0043

Alternate gene names: 218235634

Gene position: 41506-43488 (Clockwise)

Preceding gene: 218234412

Following gene: 218231319

Centisome position: 0.77

GC content: 35.7

Gene sequence:

>1983_bases
ATGACAGAGGAAAATAAGTCCTTTTATATTACTACCCCAATTTATTATCCAAGTGGAAAATTACACATTGGACATGCTTA
TACGACAGTAGCAGGAGATGCGATGGCACGATATAAGCGTATGCAAGGTTATAATGTGCATTATTTAACAGGAACTGATG
AGCATGGACAGAAGATCCAAAAGAAAGCAGAAGAGTTAAATATTACGCCACAGGCATATGTTGATAACATTGTTGCAGGA
ATTAAAGAGCTTTGGGAGAAGATGGATATTTCTTATGATGATTTTATTCGTACAACTGAAGATCGTCATAAAGATGTTGT
CGAAAAAATCTTTAAGCAATTAGTAGATCAAGGTGACATTTATCTTGATGAATATGAAGGTTGGTATTCTGTACAAGATG
AGACGTTCTATACAGAGCATCAATTAGTAGATCCAATTATGGAAGGCGACAAAGTAGTTGGAGGAAAAAGCCCAGATAGT
GGTCATGATGTAGAACTTGTTCGTGAAGAATCTTATTTCTTTAGAATGGGTAAATATGTCGACAGACTATTAAAGTTCTA
TGAAGATAATCCACATTTCATTCAGCCGGAGTCTCGTAAAAATGAAATGATTAATAATTTTATTAAACCAGGATTAGAAG
ATTTAGCAGTTTCTCGTACTTCATTTGACTGGGGAGTTCGTGTTCCTGGTAACCCAAAACACGTTATTTACGTATGGGTT
GACGCATTATCTAACTATATTACGGCGTTAGGTTATGGAACAGCAAATGAAGAGAAATATAAAAAATTCTGGCCTGCAGA
TGTGCATTTAGTTGGGAAAGAAATTGTTCGTTTCCATACAATTTATTGGCCGATAATTTTAATGGCATTAGATTTACCTC
TTCCGAAGAAAGTCTTTGCTCATGGCTGGATTTTAATGAAGGATGGAAAGATGAGTAAGTCAAAAGGAAACGTAGTAGAT
CCAGTTACATTAATCGATCGTTACGGATTAGATGCATTACGTTATTACTTACTTCGTGAAGTTCCATTTGGATCTGATGG
CGTATTTACACCAGAAGGATTTGTGGAGCGTATTAATTTCGACTTAGCAAATGACTTAGGTAATTTATTAAATCGTACAG
TAGCTATGATTGATAAGTACTTTAACGGAGAAATCCCTGCATTTAAAGCAAATGTGACGGAATTTGATGAAACGTTAGTA
GCGTTTGCACAAGATACTTTGAAAAAAGTAGAAGAAGCAATGGAGAATATGGAGTTCTCTGTAGCGTTAGGATCTATTTG
GCAACTAGTTAGTCGTACGAATAAATATATTGATGAAACACAACCATGGGTATTAGCAAAAGATGAGAATGATCGTGAAA
AGTTAGCCTCTGTAATGGCTCATTTAGCAGAAGTACTTCGTCAAACAGGTATTATGCTTATGCCATTCTTAACAGTAGCA
CCAAGCAAGATGTTTGCTCAGCTTGGCCTTACTGATGAAGCTCATAAATCTTGGGAAAGTCTATCTACAATTGGCTGCAT
TCCAGCTGGAACAAAAGTAGAAAAAGGAAACCCAATTTTCCCTCGTTTAGAAATGGAAGTGGAAGTAGAGTACATTAAAG
AACAAATGAAAAGCTCTGCTCCTAAAGTAGAAGAGAAAAAAGAAGAAGAACCAAAGGTAGAAGAAATTACAATTGATGAT
TTCTTTAAAGTAGAATTACGCGTAGCTGAAGTACTATCTGCTGAACCTGTGAAAAAAGCAGACAAGTTGTTAAAAATTCA
ACTAGACTTAGGCACAGAAAAGCGTCAAGTTGTTTCTGGAATTGCAAAATTCTATTCGCCGGAAGACCTAAAAGGTAAAA
AGGTTATTTGTGTAACAAACTTAAAACCTGTAAAATTACGCGGTGAATTATCGCAAGGTATGATTTTAGCGGGCGAAGAG
AATGGCGTATTGTCATTAGCATCAATTGACCAAAATCTACCAAATGGTACAAAAATCAAGTAA

Upstream 100 bases:

>100_bases
GGAGGAACAAAAACCCTACTGATTAAAGTTTCACTTTATATATGAGTTTTTGATATACTGTTTATAAATACATATGAGGT
TATTCGGGAGGTCCAAAACA

Downstream 100 bases:

>100_bases
TTAAGACAAGAAAAGAGATGTTTCACGTGTAACATGTGTGAAGCGTCTTTTTTCTTTTTACACTCTCTATTTTTGCATTA
AGATTGTAGTATTGTTATCG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase 1; MetRS 1 [H]

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MTEENKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRMQGYNVHYLTGTDEHGQKIQKKAEELNITPQAYVDNIVAG
IKELWEKMDISYDDFIRTTEDRHKDVVEKIFKQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVGGKSPDS
GHDVELVREESYFFRMGKYVDRLLKFYEDNPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGNPKHVIYVWV
DALSNYITALGYGTANEEKYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVD
PVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKANVTEFDETLV
AFAQDTLKKVEEAMENMEFSVALGSIWQLVSRTNKYIDETQPWVLAKDENDREKLASVMAHLAEVLRQTGIMLMPFLTVA
PSKMFAQLGLTDEAHKSWESLSTIGCIPAGTKVEKGNPIFPRLEMEVEVEYIKEQMKSSAPKVEEKKEEEPKVEEITIDD
FFKVELRVAEVLSAEPVKKADKLLKIQLDLGTEKRQVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEE
NGVLSLASIDQNLPNGTKIK

Sequences:

>Translated_660_residues
MTEENKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRMQGYNVHYLTGTDEHGQKIQKKAEELNITPQAYVDNIVAG
IKELWEKMDISYDDFIRTTEDRHKDVVEKIFKQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVGGKSPDS
GHDVELVREESYFFRMGKYVDRLLKFYEDNPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGNPKHVIYVWV
DALSNYITALGYGTANEEKYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVD
PVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKANVTEFDETLV
AFAQDTLKKVEEAMENMEFSVALGSIWQLVSRTNKYIDETQPWVLAKDENDREKLASVMAHLAEVLRQTGIMLMPFLTVA
PSKMFAQLGLTDEAHKSWESLSTIGCIPAGTKVEKGNPIFPRLEMEVEVEYIKEQMKSSAPKVEEKKEEEPKVEEITIDD
FFKVELRVAEVLSAEPVKKADKLLKIQLDLGTEKRQVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEE
NGVLSLASIDQNLPNGTKIK
>Mature_659_residues
TEENKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRMQGYNVHYLTGTDEHGQKIQKKAEELNITPQAYVDNIVAGI
KELWEKMDISYDDFIRTTEDRHKDVVEKIFKQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVGGKSPDSG
HDVELVREESYFFRMGKYVDRLLKFYEDNPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGNPKHVIYVWVD
ALSNYITALGYGTANEEKYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDP
VTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINFDLANDLGNLLNRTVAMIDKYFNGEIPAFKANVTEFDETLVA
FAQDTLKKVEEAMENMEFSVALGSIWQLVSRTNKYIDETQPWVLAKDENDREKLASVMAHLAEVLRQTGIMLMPFLTVAP
SKMFAQLGLTDEAHKSWESLSTIGCIPAGTKVEKGNPIFPRLEMEVEVEYIKEQMKSSAPKVEEKKEEEPKVEEITIDDF
FKVELRVAEVLSAEPVKKADKLLKIQLDLGTEKRQVVSGIAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEEN
GVLSLASIDQNLPNGTKIK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=505, Percent_Identity=35.049504950495, Blast_Score=308, Evalue=7e-84,
Organism=Homo sapiens, GI14043022, Length=657, Percent_Identity=26.3318112633181, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI1788432, Length=704, Percent_Identity=30.1136363636364, Blast_Score=293, Evalue=3e-80,
Organism=Escherichia coli, GI1789455, Length=109, Percent_Identity=33.9449541284404, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786861, Length=208, Percent_Identity=25.9615384615385, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI212645096, Length=491, Percent_Identity=32.7902240325866, Blast_Score=277, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI71992781, Length=383, Percent_Identity=32.1148825065274, Blast_Score=198, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17540794, Length=592, Percent_Identity=25.3378378378378, Blast_Score=166, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6321610, Length=512, Percent_Identity=31.8359375, Blast_Score=260, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6321704, Length=481, Percent_Identity=24.948024948025, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323414, Length=231, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21358483, Length=574, Percent_Identity=32.9268292682927, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24655311, Length=562, Percent_Identity=27.0462633451957, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI21355409, Length=184, Percent_Identity=27.7173913043478, Blast_Score=73, Evalue=7e-13,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75260; Mature: 75129

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEENKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRMQGYNVHYLTGTDEHGQKIQ
CCCCCCCEEEEECEEECCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
KKAEELNITPQAYVDNIVAGIKELWEKMDISYDDFIRTTEDRHKDVVEKIFKQLVDQGDI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCE
YLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVGGKSPDSGHDVELVREESYFFRMGKYV
EEECCCCEEECCCCEEEHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHH
DRLLKFYEDNPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGNPKHVIYVWV
HHHHHHHCCCCCEECCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCEECCCCCCEEEEEEH
DALSNYITALGYGTANEEKYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFA
HHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
HGWILMKDGKMSKSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINF
CCEEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCH
DLANDLGNLLNRTVAMIDKYFNGEIPAFKANVTEFDETLVAFAQDTLKKVEEAMENMEFS
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VALGSIWQLVSRTNKYIDETQPWVLAKDENDREKLASVMAHLAEVLRQTGIMLMPFLTVA
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHC
PSKMFAQLGLTDEAHKSWESLSTIGCIPAGTKVEKGNPIFPRLEMEVEVEYIKEQMKSSA
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCC
PKVEEKKEEEPKVEEITIDDFFKVELRVAEVLSAEPVKKADKLLKIQLDLGTEKRQVVSG
CCCHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCHHHHCCEEEEEECCCCHHHHHHHH
IAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEENGVLSLASIDQNLPNGTKIK
HHHHCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEECCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TEENKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRMQGYNVHYLTGTDEHGQKIQ
CCCCCCEEEEECEEECCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
KKAEELNITPQAYVDNIVAGIKELWEKMDISYDDFIRTTEDRHKDVVEKIFKQLVDQGDI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCE
YLDEYEGWYSVQDETFYTEHQLVDPIMEGDKVVGGKSPDSGHDVELVREESYFFRMGKYV
EEECCCCEEECCCCEEEHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHH
DRLLKFYEDNPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGVRVPGNPKHVIYVWV
HHHHHHHCCCCCEECCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCEECCCCCCEEEEEEH
DALSNYITALGYGTANEEKYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFA
HHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
HGWILMKDGKMSKSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINF
CCEEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCH
DLANDLGNLLNRTVAMIDKYFNGEIPAFKANVTEFDETLVAFAQDTLKKVEEAMENMEFS
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VALGSIWQLVSRTNKYIDETQPWVLAKDENDREKLASVMAHLAEVLRQTGIMLMPFLTVA
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHC
PSKMFAQLGLTDEAHKSWESLSTIGCIPAGTKVEKGNPIFPRLEMEVEVEYIKEQMKSSA
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCC
PKVEEKKEEEPKVEEITIDDFFKVELRVAEVLSAEPVKKADKLLKIQLDLGTEKRQVVSG
CCCHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCHHHHCCEEEEEECCCCHHHHHHHH
IAKFYSPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEENGVLSLASIDQNLPNGTKIK
HHHHCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEECCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]