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The map label for this gene is ymxG [H]

Identifier: 218235633

GI number: 218235633

Start: 3821789

End: 3823030

Strand: Reverse

Name: ymxG [H]

Synonym: BCB4264_A3903

Alternate gene names: 218235633

Gene position: 3823030-3821789 (Counterclockwise)

Preceding gene: 218233009

Following gene: 218231003

Centisome position: 70.55

GC content: 33.33

Gene sequence:

>1242_bases
TTGATTAAAAAATATACTTGTAAAAATGGTGTAAGAATAGTTATGGAGAATATACCAACTGTAAGATCGGTTGCAATTGG
TATTTGGATCCATGCGGGATCAAGAAATGAAAATGAAAAAAATAATGGGATTTCTCACTTTTTAGAGCATATGTTCTTTA
AAGGGACAGAAACACGTAGTGCACGAGAGATTGCAGAATCATTTGATAGCATTGGTGGTCAAGTGAATGCATTTACTTCA
AAAGAATATACATGTTACTATGCAAAAGTATTAGATGAGCATGCTAAATATGCTTTAGATGTTTTAGCAGATATGTTCTT
TAATTCAACATTTGATGAGGAAGAACTGAAAAAAGAGAAGAATGTCGTATGTGAAGAAATTAAAATGTACGAAGATGCTC
CAGATGATATTGTGCATGATATGTTAACGAAAGCAACATATGAAACGCATCCACTTGGATATCCTATTTTAGGAACAGAA
GAAACGCTTAATACGTTTACAGGTGATACGTTACGTCAATATATAAAAGATCATTATACACCTGAAAATGTAGTTGTATC
AATCGCAGGAAATATTGATGAAGCATTTTTACAAACAGTGGAGCAATATTTCGGTAGTTATGAAGGAACAACAAACCGTG
AACAAGTACATAGCCCAATTTTCCATTTCAATAAGGTAGCCCGTAAAAAAGAAACGGAACAAGCTCATTTATGTCTAGGA
TATAAAGGCTTACAAATGGGACACGAAGATATTTATAACTTAATTGTATTAAATAATGTTTTAGGCGGTAGCATGAGTAG
CCGTTTATTCCAAGAAGTACGTGAGCAACGTGGATTAGCTTACTCAGTATTTTCTTACCACTCTTCTTATGAAGATACAG
GCATGTTAACACTTTACGGTGGAACAGGTAGCCAACAATTAGATACATTGTATGAAACAATGCAAGAAACATTAGAGACA
TTGAAAAATACAGGTATTACAGAAAAAGAGCTTATGAATAGTAAAGAGCAATTAAAAGGAAACTTAATGTTAAGTTTAGA
AAGTACAAATAGCCGTATGAGCCGTAACGGTAAAAATGAATTGCTTCTTCGTAAACATCGTTCACTTGATGAGATTATTG
AAAGTGTAAACACTGTAACAAAAGAAAATGTGGACGGATTAATTCGCAATATGTTTACTGACGAGTTCTCTGCGGCACTT
ATTAGTCCAGACGGAAAACTTCCAAAAGGAATAAAACTATAA

Upstream 100 bases:

>100_bases
ACGTGTGGATTAAATACATTTGCATGACATTCATCCTTAAACTTGTTATCATTAAATAATAGCGTTTTATTTAGAGAAAC
TGGCAGTAGGAGGAAAGTTT

Downstream 100 bases:

>100_bases
TCAATTTTTACGATGTATAAAAAATAACCTTCTCTCTTTTTAAATGAGAGAAGGTTATTTTTTTTATACATATAGAATAG
GTACTAATTATAGACAATGC

Product: zinc protease, insulinase family

Products: NA

Alternate protein names: ORFP [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MIKKYTCKNGVRIVMENIPTVRSVAIGIWIHAGSRNENEKNNGISHFLEHMFFKGTETRSAREIAESFDSIGGQVNAFTS
KEYTCYYAKVLDEHAKYALDVLADMFFNSTFDEEELKKEKNVVCEEIKMYEDAPDDIVHDMLTKATYETHPLGYPILGTE
ETLNTFTGDTLRQYIKDHYTPENVVVSIAGNIDEAFLQTVEQYFGSYEGTTNREQVHSPIFHFNKVARKKETEQAHLCLG
YKGLQMGHEDIYNLIVLNNVLGGSMSSRLFQEVREQRGLAYSVFSYHSSYEDTGMLTLYGGTGSQQLDTLYETMQETLET
LKNTGITEKELMNSKEQLKGNLMLSLESTNSRMSRNGKNELLLRKHRSLDEIIESVNTVTKENVDGLIRNMFTDEFSAAL
ISPDGKLPKGIKL

Sequences:

>Translated_413_residues
MIKKYTCKNGVRIVMENIPTVRSVAIGIWIHAGSRNENEKNNGISHFLEHMFFKGTETRSAREIAESFDSIGGQVNAFTS
KEYTCYYAKVLDEHAKYALDVLADMFFNSTFDEEELKKEKNVVCEEIKMYEDAPDDIVHDMLTKATYETHPLGYPILGTE
ETLNTFTGDTLRQYIKDHYTPENVVVSIAGNIDEAFLQTVEQYFGSYEGTTNREQVHSPIFHFNKVARKKETEQAHLCLG
YKGLQMGHEDIYNLIVLNNVLGGSMSSRLFQEVREQRGLAYSVFSYHSSYEDTGMLTLYGGTGSQQLDTLYETMQETLET
LKNTGITEKELMNSKEQLKGNLMLSLESTNSRMSRNGKNELLLRKHRSLDEIIESVNTVTKENVDGLIRNMFTDEFSAAL
ISPDGKLPKGIKL
>Mature_413_residues
MIKKYTCKNGVRIVMENIPTVRSVAIGIWIHAGSRNENEKNNGISHFLEHMFFKGTETRSAREIAESFDSIGGQVNAFTS
KEYTCYYAKVLDEHAKYALDVLADMFFNSTFDEEELKKEKNVVCEEIKMYEDAPDDIVHDMLTKATYETHPLGYPILGTE
ETLNTFTGDTLRQYIKDHYTPENVVVSIAGNIDEAFLQTVEQYFGSYEGTTNREQVHSPIFHFNKVARKKETEQAHLCLG
YKGLQMGHEDIYNLIVLNNVLGGSMSSRLFQEVREQRGLAYSVFSYHSSYEDTGMLTLYGGTGSQQLDTLYETMQETLET
LKNTGITEKELMNSKEQLKGNLMLSLESTNSRMSRNGKNELLLRKHRSLDEIIESVNTVTKENVDGLIRNMFTDEFSAAL
ISPDGKLPKGIKL

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family [H]

Homologues:

Organism=Homo sapiens, GI94538354, Length=420, Percent_Identity=29.7619047619048, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI46593007, Length=415, Percent_Identity=25.0602409638554, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI24308013, Length=459, Percent_Identity=24.6187363834423, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI50592988, Length=425, Percent_Identity=20.7058823529412, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI155969707, Length=119, Percent_Identity=36.1344537815126, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787770, Length=177, Percent_Identity=28.8135593220339, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI2367164, Length=172, Percent_Identity=26.1627906976744, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71999683, Length=416, Percent_Identity=28.6057692307692, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17553678, Length=418, Percent_Identity=25.8373205741627, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17510601, Length=368, Percent_Identity=22.2826086956522, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323192, Length=394, Percent_Identity=30.7106598984772, Blast_Score=200, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6321813, Length=427, Percent_Identity=26.6978922716628, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6319426, Length=411, Percent_Identity=21.8978102189781, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21357875, Length=412, Percent_Identity=29.6116504854369, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24646943, Length=412, Percent_Identity=29.6116504854369, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI19921772, Length=439, Percent_Identity=24.8291571753986, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24665395, Length=406, Percent_Identity=21.1822660098522, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- InterPro:   IPR001431
- InterPro:   IPR007863 [H]

Pfam domain/function: PF00675 Peptidase_M16; PF05193 Peptidase_M16_C [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 46951; Mature: 46951

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00143 INSULINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKYTCKNGVRIVMENIPTVRSVAIGIWIHAGSRNENEKNNGISHFLEHMFFKGTETRS
CCCCEECCCCCEEHHHCCCHHHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHH
AREIAESFDSIGGQVNAFTSKEYTCYYAKVLDEHAKYALDVLADMFFNSTFDEEELKKEK
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NVVCEEIKMYEDAPDDIVHDMLTKATYETHPLGYPILGTEETLNTFTGDTLRQYIKDHYT
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCC
PENVVVSIAGNIDEAFLQTVEQYFGSYEGTTNREQVHSPIFHFNKVARKKETEQAHLCLG
CCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHEEC
YKGLQMGHEDIYNLIVLNNVLGGSMSSRLFQEVREQRGLAYSVFSYHSSYEDTGMLTLYG
CHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCCEEEEEC
GTGSQQLDTLYETMQETLETLKNTGITEKELMNSKEQLKGNLMLSLESTNSRMSRNGKNE
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHCCCCEEEEEECCCHHHHCCCCHH
LLLRKHRSLDEIIESVNTVTKENVDGLIRNMFTDEFSAALISPDGKLPKGIKL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCC
>Mature Secondary Structure
MIKKYTCKNGVRIVMENIPTVRSVAIGIWIHAGSRNENEKNNGISHFLEHMFFKGTETRS
CCCCEECCCCCEEHHHCCCHHHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHH
AREIAESFDSIGGQVNAFTSKEYTCYYAKVLDEHAKYALDVLADMFFNSTFDEEELKKEK
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NVVCEEIKMYEDAPDDIVHDMLTKATYETHPLGYPILGTEETLNTFTGDTLRQYIKDHYT
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCC
PENVVVSIAGNIDEAFLQTVEQYFGSYEGTTNREQVHSPIFHFNKVARKKETEQAHLCLG
CCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHEEC
YKGLQMGHEDIYNLIVLNNVLGGSMSSRLFQEVREQRGLAYSVFSYHSSYEDTGMLTLYG
CHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCCEEEEEC
GTGSQQLDTLYETMQETLETLKNTGITEKELMNSKEQLKGNLMLSLESTNSRMSRNGKNE
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHCCCCEEEEEECCCHHHHCCCCHH
LLLRKHRSLDEIIESVNTVTKENVDGLIRNMFTDEFSAALISPDGKLPKGIKL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 8098035 [H]