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The map label for this gene is pit [H]

Identifier: 218235631

GI number: 218235631

Start: 1442048

End: 1443046

Strand: Reverse

Name: pit [H]

Synonym: BCB4264_A1508

Alternate gene names: 218235631

Gene position: 1443046-1442048 (Counterclockwise)

Preceding gene: 218230938

Following gene: 218234248

Centisome position: 26.63

GC content: 38.04

Gene sequence:

>999_bases
ATGGATACACTCTTGATACTAACCGTTTTAGTAGTCATTTGCGCTTTAGCTTTTGACTTTATCAATGGATTTCACGATAC
AGCAAACGCTATTGCAACGGCTGTTTCAACGAAAGCTCTAAAGCCTAGACATGCCATTATTATGGCGGCTATTATGAACT
TTTTAGGCGCAATGACATTTACAGGTGTAGCAAAAACGATTACAAAAGATATCGTTGATCCATTCGCCTTACAAAATGGT
TCTCTTGTAATTTTAGCTGCTTTGCTTGCGGCGATAGCTTGGAACTTAATTACTTGGTACTACGGTATTCCAAGTAGTTC
TTCGCATGCAATTATCGGCTCAATCGCAGGTGCAGCAATTGCGGCCGCTGGGATTAGCTCGCTAAATCTTAAAGGATTTA
TTAAAATTATTGAAGCTTTAATCATTTCACCGATTATCGCTTTTGTTATTGGTTATACCGTATACAGTATTTTTAAAGTA
GTATTTAAAAACTTTAACTTAACAAAAACGAATAAGAACTTCCGTATATTCCAGATTTTCACTGCAGCATTACAAGCCTA
CACACACGGTACGAATGATGCACAAAAAGCAATGGGAATCATTACGATGGCTCTTATGGCTAATAACTATCATACTTCTA
GCGATATCCCGTTCTGGGTACAATTATCTTGTGCTATCGCAATGGGGCTTGGTACTTCTGTCGGCGGATGGAAAATTATT
AAAACTGTCGGTGGACAAATTATGAAAATTCGTCCTGTAAATGGTGTAGCTGCCGATTTATCATCATCACTTGTTATTTT
CGGTGCAACATTCATTCACTTACCGGTTAGTACGACGCACGTTATCTCTTCTTCTATTTTAGGGGTTGGTGCTTCTCACC
GTGTGAAAGGGGTAAAATGGGGAACTGCAAAACGCATGTTAATTACATGGGTTATTACACTTCCTATTTCAGCTTCTTTA
GCTGCTCTGTTTTACTACCTATTACATTTTGTTTTCTAA

Upstream 100 bases:

>100_bases
AAGAGATTTACGAAGAGCTTGAAGAAGTAGCTGATAGCTGTCAAAGCGTTGCAAACGTATTAGAAACAATTATTATGAAG
AACGCGTAAGGAGTTTGATC

Downstream 100 bases:

>100_bases
TCAAAAGTCGTCTTCCTTATTTTTCTAGGAAGACGACTTTTTTGTGAACAAGCCTATTTCTCATTAAAAAGCGTGTATAA
TAATTGGTGGATAAGTTATT

Product: phosphate transporter family protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MDTLLILTVLVVICALAFDFINGFHDTANAIATAVSTKALKPRHAIIMAAIMNFLGAMTFTGVAKTITKDIVDPFALQNG
SLVILAALLAAIAWNLITWYYGIPSSSSHAIIGSIAGAAIAAAGISSLNLKGFIKIIEALIISPIIAFVIGYTVYSIFKV
VFKNFNLTKTNKNFRIFQIFTAALQAYTHGTNDAQKAMGIITMALMANNYHTSSDIPFWVQLSCAIAMGLGTSVGGWKII
KTVGGQIMKIRPVNGVAADLSSSLVIFGATFIHLPVSTTHVISSSILGVGASHRVKGVKWGTAKRMLITWVITLPISASL
AALFYYLLHFVF

Sequences:

>Translated_332_residues
MDTLLILTVLVVICALAFDFINGFHDTANAIATAVSTKALKPRHAIIMAAIMNFLGAMTFTGVAKTITKDIVDPFALQNG
SLVILAALLAAIAWNLITWYYGIPSSSSHAIIGSIAGAAIAAAGISSLNLKGFIKIIEALIISPIIAFVIGYTVYSIFKV
VFKNFNLTKTNKNFRIFQIFTAALQAYTHGTNDAQKAMGIITMALMANNYHTSSDIPFWVQLSCAIAMGLGTSVGGWKII
KTVGGQIMKIRPVNGVAADLSSSLVIFGATFIHLPVSTTHVISSSILGVGASHRVKGVKWGTAKRMLITWVITLPISASL
AALFYYLLHFVF
>Mature_332_residues
MDTLLILTVLVVICALAFDFINGFHDTANAIATAVSTKALKPRHAIIMAAIMNFLGAMTFTGVAKTITKDIVDPFALQNG
SLVILAALLAAIAWNLITWYYGIPSSSSHAIIGSIAGAAIAAAGISSLNLKGFIKIIEALIISPIIAFVIGYTVYSIFKV
VFKNFNLTKTNKNFRIFQIFTAALQAYTHGTNDAQKAMGIITMALMANNYHTSSDIPFWVQLSCAIAMGLGTSVGGWKII
KTVGGQIMKIRPVNGVAADLSSSLVIFGATFIHLPVSTTHVISSSILGVGASHRVKGVKWGTAKRMLITWVITLPISASL
AALFYYLLHFVF

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=162, Percent_Identity=32.0987654320988, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI5803173, Length=166, Percent_Identity=28.9156626506024, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI1789360, Length=218, Percent_Identity=32.5688073394495, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1789907, Length=218, Percent_Identity=32.1100917431193, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI25146401, Length=154, Percent_Identity=33.7662337662338, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17539280, Length=164, Percent_Identity=29.2682926829268, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71981576, Length=170, Percent_Identity=31.7647058823529, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32566716, Length=184, Percent_Identity=28.2608695652174, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17536725, Length=168, Percent_Identity=26.7857142857143, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319773, Length=154, Percent_Identity=27.9220779220779, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21356511, Length=183, Percent_Identity=25.6830601092896, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35459; Mature: 35459

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLLILTVLVVICALAFDFINGFHDTANAIATAVSTKALKPRHAIIMAAIMNFLGAMTF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TGVAKTITKDIVDPFALQNGSLVILAALLAAIAWNLITWYYGIPSSSSHAIIGSIAGAAI
HHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AAAGISSLNLKGFIKIIEALIISPIIAFVIGYTVYSIFKVVFKNFNLTKTNKNFRIFQIF
HHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEHHHHH
TAALQAYTHGTNDAQKAMGIITMALMANNYHTSSDIPFWVQLSCAIAMGLGTSVGGWKII
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHCCCCCCCHHHH
KTVGGQIMKIRPVNGVAADLSSSLVIFGATFIHLPVSTTHVISSSILGVGASHRVKGVKW
HHCCCCEEEEEECCCHHHHHCCCEEEEEHEEEEECCCHHHHHHHHHHCCCCCCCCCCCCC
GTAKRMLITWVITLPISASLAALFYYLLHFVF
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDTLLILTVLVVICALAFDFINGFHDTANAIATAVSTKALKPRHAIIMAAIMNFLGAMTF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TGVAKTITKDIVDPFALQNGSLVILAALLAAIAWNLITWYYGIPSSSSHAIIGSIAGAAI
HHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AAAGISSLNLKGFIKIIEALIISPIIAFVIGYTVYSIFKVVFKNFNLTKTNKNFRIFQIF
HHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEHHHHH
TAALQAYTHGTNDAQKAMGIITMALMANNYHTSSDIPFWVQLSCAIAMGLGTSVGGWKII
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHCCCCCCCHHHH
KTVGGQIMKIRPVNGVAADLSSSLVIFGATFIHLPVSTTHVISSSILGVGASHRVKGVKW
HHCCCCEEEEEECCCHHHHHCCCEEEEEHEEEEECCCHHHHHHHHHHCCCCCCCCCCCCC
GTAKRMLITWVITLPISASLAALFYYLLHFVF
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]