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The map label for this gene is proS1 [H]

Identifier: 218235284

GI number: 218235284

Start: 445204

End: 446634

Strand: Direct

Name: proS1 [H]

Synonym: BCB4264_A0461

Alternate gene names: 218235284

Gene position: 445204-446634 (Clockwise)

Preceding gene: 218233714

Following gene: 218234106

Centisome position: 8.22

GC content: 36.48

Gene sequence:

>1431_bases
ATGGCAAAAGAACAAGTGCAAGCCATTACGAAGATGGAAGAGGACTTTGCGCAGTGGTATACCGATATTGTAAAAAAAGC
CGAACTTGTTGATTATTCAAGTGTAAAAGGATGCATGATTTTACGTCCGTATGGTTATGCCTTATGGGAGAATATGCAGA
AGGTGATGGATGAGAAGTTAAAAGAAACTGGCCATGAAAATGTATATATGCCAATGTTTATCCCAGAGAGTTTATTGCAA
AAAGAGAAGGATCATGTTGAAGGATTTGCTCCTGAAGTAGCATGGGTTACACATGGCGGGGATGAAAAGTTAGCGGAAAG
ACTTTGTGTACGTCCTACATCTGAAACTTTATTCTGTGAGCACTTTTCAAAAATTGTGCAATCCTATAATGATTTGCCAA
AGTTATATAATCAGTGGTGTTCAGTAGTTCGTTGGGAGAAGACAACTAGACCATTCCTTCGTACGACTGAATTTTTATGG
CAAGAGGGTCATACAATCCATGAAACGGCAGAAGAATCGCAGGCTGAAACATTAAATATTTTAAACTTATATGCTTCTTT
CTGTGAGGATTATTTAGCAATACCAGTTATTAAAGGACAAAAAACAGAAAAAGAGAAGTTTGCTGGAGCAAAGGCAACTT
ATACAATTGAAAGTTTAATGCATGATGGAAAAGCACTTCAAACAGGAACCTCTCATAACTTTGGAACGAATTTCTCTAAA
GCATTTGATATTAAGTTTTTAGATCGTAACGGTAAGTGGCAATATGTGCACCAAACATCTTGGGGTGTATCAACAAGAAT
GATAGGCGGGCTAATAATGGTTCATAGTGATAATAATGGACTTGTAATGCCACCAAAAGTCGCTCCAGTGCAAGTTGTTA
TTGTACCAATTGCTCAGCATAAAGAAGGAGTTTTAGCAAAAGCAACAGAGTTACAAGCACATATTCAAAAGGTTGCACGA
GTAAAAATAGATGCTAGTAATAAAACACCAGGCTGGAAATTTAATGAGTATGAAATGAAGGGGATTCCAATTCGATTAGA
AGTTGGCCCTAAAGATATTGAAAAGAATCAAGTTGTACTTGTAAGAAGAGATACGAAGGAAAAAGAGTTTGTACCAATGG
ATCAATTAGAAGAACGTATTCCAGCATTACTTGAAGAAATTCATATTGCTTTATTTAATAAAGCAAAAGCATTTCGCGAT
GAGAATACGTATGTTGCGACAAATTTCGAAGAGATGAAAAAAATAGCTGACGAAAAGCAAGGCTTTATTAAGGCAATGTG
GTGCGGGGAATTAGCTTGTGAAGAAAAACTAAAAGAAGAAGTTGGCGTATCTTCACGTTGTATGCCTTTTGAGCAAGAGC
ATTTAGCTGAAGAATGTATTTGTTGTGGTAAAGAAGCTAAACAAATGGTGTATTGGGGAAAAGCGTATTAA

Upstream 100 bases:

>100_bases
CAATTTTTGTGCATGAATCAGGGTGGTAACGCCGGCAAAGCTCGGTCCCTATTTAGGGACGCGGGCTTTTTTGTATTTTC
TAGAAGGAGGAAGACTGACT

Downstream 100 bases:

>100_bases
TACTTTTTCTGATAGGTGATAAAAGGACTTTGGGCTGCATAATAAAATGTAATCCAAAGTCCTTTTTAGTTTTATACAGT
ATTTCATGTACTTTTTAGAT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase 2; ProRS 2 [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MAKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKLKETGHENVYMPMFIPESLLQ
KEKDHVEGFAPEVAWVTHGGDEKLAERLCVRPTSETLFCEHFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLW
QEGHTIHETAEESQAETLNILNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSK
AFDIKFLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQHKEGVLAKATELQAHIQKVAR
VKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVLVRRDTKEKEFVPMDQLEERIPALLEEIHIALFNKAKAFRD
ENTYVATNFEEMKKIADEKQGFIKAMWCGELACEEKLKEEVGVSSRCMPFEQEHLAEECICCGKEAKQMVYWGKAY

Sequences:

>Translated_476_residues
MAKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKLKETGHENVYMPMFIPESLLQ
KEKDHVEGFAPEVAWVTHGGDEKLAERLCVRPTSETLFCEHFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLW
QEGHTIHETAEESQAETLNILNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSK
AFDIKFLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQHKEGVLAKATELQAHIQKVAR
VKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVLVRRDTKEKEFVPMDQLEERIPALLEEIHIALFNKAKAFRD
ENTYVATNFEEMKKIADEKQGFIKAMWCGELACEEKLKEEVGVSSRCMPFEQEHLAEECICCGKEAKQMVYWGKAY
>Mature_475_residues
AKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKLKETGHENVYMPMFIPESLLQK
EKDHVEGFAPEVAWVTHGGDEKLAERLCVRPTSETLFCEHFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQ
EGHTIHETAEESQAETLNILNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSKA
FDIKFLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQHKEGVLAKATELQAHIQKVARV
KIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVLVRRDTKEKEFVPMDQLEERIPALLEEIHIALFNKAKAFRDE
NTYVATNFEEMKKIADEKQGFIKAMWCGELACEEKLKEEVGVSSRCMPFEQEHLAEECICCGKEAKQMVYWGKAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=495, Percent_Identity=45.8585858585859, Blast_Score=440, Evalue=1e-123,
Organism=Escherichia coli, GI1786392, Length=151, Percent_Identity=25.8278145695364, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71991342, Length=490, Percent_Identity=42.4489795918367, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71991346, Length=357, Percent_Identity=46.218487394958, Blast_Score=356, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6321808, Length=501, Percent_Identity=47.5049900199601, Blast_Score=450, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24649468, Length=494, Percent_Identity=43.3198380566802, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24649466, Length=494, Percent_Identity=43.3198380566802, Blast_Score=410, Evalue=1e-114,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 54716; Mature: 54585

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
KETGHENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTHGGDEKLAERLCVRPTSETLFCE
HHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHH
HFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETAEESQAETLNI
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSK
HHHHHHHHHHHHCCEEECCCCCHHHHHCCCCHHEEHHHHHCCCCCEECCCCCCCCCCCCC
AFDIKFLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQH
EEEEEEECCCCCEEEEEECCCCHHHHHHCCEEEEEECCCCEEECCCCCCEEEEEEEECCC
KEGVLAKATELQAHIQKVARVKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVL
CCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCEEE
VRRDTKEKEFVPMDQLEERIPALLEEIHIALFNKAKAFRDENTYVATNFEEMKKIADEKQ
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHC
GFIKAMWCGELACEEKLKEEVGVSSRCMPFEQEHLAEECICCGKEAKQMVYWGKAY
CCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHEEECCCCC
>Mature Secondary Structure 
AKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENMQKVMDEKL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
KETGHENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTHGGDEKLAERLCVRPTSETLFCE
HHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHH
HFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETAEESQAETLNI
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LNLYASFCEDYLAIPVIKGQKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSK
HHHHHHHHHHHHCCEEECCCCCHHHHHCCCCHHEEHHHHHCCCCCEECCCCCCCCCCCCC
AFDIKFLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVMPPKVAPVQVVIVPIAQH
EEEEEEECCCCCEEEEEECCCCHHHHHHCCEEEEEECCCCEEECCCCCCEEEEEEEECCC
KEGVLAKATELQAHIQKVARVKIDASNKTPGWKFNEYEMKGIPIRLEVGPKDIEKNQVVL
CCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCEEE
VRRDTKEKEFVPMDQLEERIPALLEEIHIALFNKAKAFRDENTYVATNFEEMKKIADEKQ
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHC
GFIKAMWCGELACEEKLKEEVGVSSRCMPFEQEHLAEECICCGKEAKQMVYWGKAY
CCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]