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The map label for this gene is nusA [H]

Identifier: 218235265

GI number: 218235265

Start: 3832176

End: 3833282

Strand: Reverse

Name: nusA [H]

Synonym: BCB4264_A3914

Alternate gene names: 218235265

Gene position: 3833282-3832176 (Counterclockwise)

Preceding gene: 218235854

Following gene: 218232577

Centisome position: 70.74

GC content: 38.75

Gene sequence:

>1107_bases
ATGAGCACTGAGTTATTAGATGCTTTGCTCGTATTAGAATCTGAAAAAGGCATTAGCAAAGACATTATTATTGATGCGAT
TGAAGCAGCTTTAATCTCTGCTTATAAGCGCAATTTTAATCAAGCACAAAACGTTCGTGTGAGCTTTAACCCAGAAGTGG
GAACAATTCAAGTTTTAGCACGTAAAGACGTAGTAGACAATGTGTTTGATCCACGTCTTGAAATTTCTGTAGAGGAAGCA
AGACAAATTAATCCAAACTATCAAGATGGTGACGTACTAGAAATTGAAGTAACACCAAAAGACTTTGGTCGTATTGCAGC
ACAAACTGCAAAACAAGTTGTAACACAACGAGTGCGTGAAGCAGAGCGCGGTGTTATTTACTCTGAGTTTAGTGACCGTG
AAGAAGATATTATGGTAGGTATTGTACAACGTCAAGATGCTCGTTTCATCTATGTAAGCTTAGGAAAAGTAGAAGCTTTA
TTACCTGTAAGTGAGCAAATGCCTAATGAGCAATACAAACCACATGATCGTATTCGCGTATTCATTACGAAAGTAGAAAA
AACGACAAAAGGACCACAAATTTACGTATCACGTACACATCCTGGTCTTTTAAAACGTTTATTCGAAATGGAAGTTCCTG
AAATCTATGATGGAACTGTAGAAATTCGTTCTGTAGCACGTGAAGCTGGCGATCGCTCTAAGATCTCTGTATATGCAGAG
AACACTGATGTTGATCCAGTTGGTTCTTGTGTAGGACCAAAAGGGCAACGTGTACAACGCATTGTAGACGAATTAAAAGG
TGAAAAAATTGACATCGTTCGCTGGTCAAATGATCCAGTTGAATATGTTGCAAATGCATTAAGCCCATCACAAGTTGTTA
AAGTACTTGTAGATGAAGAAGAAAAAGCGACAACAGTAGTTGTTCCAGACCACCAGCTATCATTAGCTATCGGTAAGCGT
GGACAAAATGCACGTCTTGCAGCTAAATTAACAGGCTGGAAAATTGACATTAAGAGTGAGTCTGATGCGAAACAACTTGG
TATTGTAACAGAAGAAGATAGCGTAATCGCATTCGGATTCGATTCAGTGGAAGACGAAATCGAATAG

Upstream 100 bases:

>100_bases
TATTAACAATTAAAACACGTAAAAAAGAAATCCAAATTTCGATGGATAAAGTCGCAAATGCGCGACTTGCTGTTACGTTT
TAGTTAGAAGGGGGATCTTC

Downstream 100 bases:

>100_bases
AGGTGATGAGTATGAGCAATCGAAAAGTTCCGTTACGAAAATGTGTTGCAACGCAAGAAATGAAATCAAAACGAGAGCTC
GTTCGCATTGTTCGTTCCAA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSTELLDALLVLESEKGISKDIIIDAIEAALISAYKRNFNQAQNVRVSFNPEVGTIQVLARKDVVDNVFDPRLEISVEEA
RQINPNYQDGDVLEIEVTPKDFGRIAAQTAKQVVTQRVREAERGVIYSEFSDREEDIMVGIVQRQDARFIYVSLGKVEAL
LPVSEQMPNEQYKPHDRIRVFITKVEKTTKGPQIYVSRTHPGLLKRLFEMEVPEIYDGTVEIRSVAREAGDRSKISVYAE
NTDVDPVGSCVGPKGQRVQRIVDELKGEKIDIVRWSNDPVEYVANALSPSQVVKVLVDEEEKATTVVVPDHQLSLAIGKR
GQNARLAAKLTGWKIDIKSESDAKQLGIVTEEDSVIAFGFDSVEDEIE

Sequences:

>Translated_368_residues
MSTELLDALLVLESEKGISKDIIIDAIEAALISAYKRNFNQAQNVRVSFNPEVGTIQVLARKDVVDNVFDPRLEISVEEA
RQINPNYQDGDVLEIEVTPKDFGRIAAQTAKQVVTQRVREAERGVIYSEFSDREEDIMVGIVQRQDARFIYVSLGKVEAL
LPVSEQMPNEQYKPHDRIRVFITKVEKTTKGPQIYVSRTHPGLLKRLFEMEVPEIYDGTVEIRSVAREAGDRSKISVYAE
NTDVDPVGSCVGPKGQRVQRIVDELKGEKIDIVRWSNDPVEYVANALSPSQVVKVLVDEEEKATTVVVPDHQLSLAIGKR
GQNARLAAKLTGWKIDIKSESDAKQLGIVTEEDSVIAFGFDSVEDEIE
>Mature_367_residues
STELLDALLVLESEKGISKDIIIDAIEAALISAYKRNFNQAQNVRVSFNPEVGTIQVLARKDVVDNVFDPRLEISVEEAR
QINPNYQDGDVLEIEVTPKDFGRIAAQTAKQVVTQRVREAERGVIYSEFSDREEDIMVGIVQRQDARFIYVSLGKVEALL
PVSEQMPNEQYKPHDRIRVFITKVEKTTKGPQIYVSRTHPGLLKRLFEMEVPEIYDGTVEIRSVAREAGDRSKISVYAEN
TDVDPVGSCVGPKGQRVQRIVDELKGEKIDIVRWSNDPVEYVANALSPSQVVKVLVDEEEKATTVVVPDHQLSLAIGKRG
QNARLAAKLTGWKIDIKSESDAKQLGIVTEEDSVIAFGFDSVEDEIE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=343, Percent_Identity=37.9008746355685, Blast_Score=258, Evalue=4e-70,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 41179; Mature: 41048

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTELLDALLVLESEKGISKDIIIDAIEAALISAYKRNFNQAQNVRVSFNPEVGTIQVLA
CCHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCCEEEEEE
RKDVVDNVFDPRLEISVEEARQINPNYQDGDVLEIEVTPKDFGRIAAQTAKQVVTQRVRE
HHHHHHHHCCCCEEEEHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
AERGVIYSEFSDREEDIMVGIVQRQDARFIYVSLGKVEALLPVSEQMPNEQYKPHDRIRV
HHCCEEEECCCCCCCCEEEEEEECCCCCEEEEEECCEEEECCCHHHCCCCCCCCCCCEEE
FITKVEKTTKGPQIYVSRTHPGLLKRLFEMEVPEIYDGTVEIRSVAREAGDRSKISVYAE
EEEEEHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEC
NTDVDPVGSCVGPKGQRVQRIVDELKGEKIDIVRWSNDPVEYVANALSPSQVVKVLVDEE
CCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCHHHHHHHEECCC
EKATTVVVPDHQLSLAIGKRGQNARLAAKLTGWKIDIKSESDAKQLGIVTEEDSVIAFGF
CCCEEEEECCCEEEEEECCCCCCCEEEEEEECEEEEECCCCCHHHCCEEECCCCEEEEEC
DSVEDEIE
CCCHHCCC
>Mature Secondary Structure 
STELLDALLVLESEKGISKDIIIDAIEAALISAYKRNFNQAQNVRVSFNPEVGTIQVLA
CHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCCEEEEEE
RKDVVDNVFDPRLEISVEEARQINPNYQDGDVLEIEVTPKDFGRIAAQTAKQVVTQRVRE
HHHHHHHHCCCCEEEEHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
AERGVIYSEFSDREEDIMVGIVQRQDARFIYVSLGKVEALLPVSEQMPNEQYKPHDRIRV
HHCCEEEECCCCCCCCEEEEEEECCCCCEEEEEECCEEEECCCHHHCCCCCCCCCCCEEE
FITKVEKTTKGPQIYVSRTHPGLLKRLFEMEVPEIYDGTVEIRSVAREAGDRSKISVYAE
EEEEEHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEC
NTDVDPVGSCVGPKGQRVQRIVDELKGEKIDIVRWSNDPVEYVANALSPSQVVKVLVDEE
CCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCHHHHHHHEECCC
EKATTVVVPDHQLSLAIGKRGQNARLAAKLTGWKIDIKSESDAKQLGIVTEEDSVIAFGF
CCCEEEEECCCEEEEEECCCCCCCEEEEEEECEEEEECCCCCHHHCCEEECCCCEEEEEC
DSVEDEIE
CCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]