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The map label for this gene is ydaP [H]

Identifier: 218235075

GI number: 218235075

Start: 2264316

End: 2266016

Strand: Reverse

Name: ydaP [H]

Synonym: BCB4264_A2360

Alternate gene names: 218235075

Gene position: 2266016-2264316 (Counterclockwise)

Preceding gene: 218231172

Following gene: 218235073

Centisome position: 41.82

GC content: 38.33

Gene sequence:

>1701_bases
ATGTTTGGGAAAACAGCCGGTGAAGCATTAGTTGATTTATTAATTGACTGGGGAGTAGAACACATATACGGCATGCCTGG
AGATTCCATTAATTCTATCATTGAAGCTTTAAGAAAAAAACAAGATAAAATTAAATTCATTCAAGTTCGCCATGAAGAAG
CTGGTGCATTAGCAGCGGCTGCCTATGCGAAATTAACAGGAAAACTTGGCGTTTGTATGGCAATTGCAGGACCTGGTGCT
ATTCATTTATTAAATGGTTTATATGATGCGAAACTCGATAAAGCCCCTGTTCTAGCAATTTCAGGTCAAGTAGAAACCGA
TTTACTTGGAACGGACTTTTTCCAAGAAGTAAATTTAGAAAGATTGTTTGATGATGTTGCGGTTTATAACCAGCGTATTA
TGTCAGCAGAGCAACTACCTGCCGTAGTGAATCAAGCAATTCGAATGGCTTATACGAAAAAAGGTGTATCCGTTCTTACA
ATTCCAGATGATGTCCCTAAATTCGAAGTAAAAAATGGTGCCCGTGTTACAAATACTTCTTTTACTTTGCCTGAGTTGTT
TCCACAAAAAAACGATTTAGATGCAGCGAAACTCGCAATAAATGAAGCGAAAAGGCCTGTGATTTTAGCTGGAAAAGGAA
CAAAACATGCGAGAGAATCTTTACTTGCATTGGCAGAACATATTGGTGCTCCAATCGTACTTACATTACCAGCTAAAGGA
ATTATTCCTGATGAACACCCTCTTTGTATTGGTGGATTAGGATTAATTGGAACGAAACCTTCTTATGAAGCAATGAAGCA
TGCAGATACTTTAATTATGGTCGGTACTTCTTATCCGTTCACCGGCTTCTTACCTGAAAAAGCAAAAACAATTCATATCG
ATACAGATCCTGCCCAAATCGGGAAACGTTATGCGACAGATATTGGTTTAGCTGGTGATGCTGATAAAACATTACGATGG
TTAATTGAAAATGTTGAAGAACACACAGACCATTCCTTCTTAGAACATCACCAAGAAATGATGAAAAAGTGGGAAGAAAA
ATTACACGATCAAGAAGAAGACTCTTCCATTCCAATTAAACCACAACGCGTTATGCATGCTCTGCAGCAAGTAGCACATG
ATGACGCAATTCTATCAGTCGATGTCGGAAATGTAACAGTATGGACCGCAAGACACTTCCGTATGACAAACCAGCAATTT
GTCATTTCTAGCTGGCTCGCAACACTTGGTTGCGGTTTACCTGGTGCTCTTGCTGGAAAAATTGCATTCCCGGATAAACA
AGTATTTGCAGTTTGTGGTGATGGCGGATTTGGAATGACGATGAATGACTTCGTAACAGCTGTAAAATATAAATTGCCAA
TCGTTGTCGTCGTATTAAACAATAATAAAATTGCGATGATTAAATTCGAGCAAGAAGTTATGGGTAATATCGAATTCGAA
ACAGATTTAACGAACCCTGATTTTGCAAAATATGCAGAGGCGTGTGGAGGTATTGGATACCGTGTTGAGAATTTAGATGA
ACTCCTTCCCGCTTTCGAAAATGCAGTAAAACAAAATAAACCGTGTATTATTGATGTAGTTGTAGATGCTAACGAAGCAC
CAATGCCAGCAAAAATCACATTTGGTCAAGCCGCTGGTTATACGAAGCATATGATAAAAGAATTATTTGAAGAAGGTAAG
ATTGATTTACCTCCACTTTAA

Upstream 100 bases:

>100_bases
ATTCTTGGATTACAAATAACCAGATAAATTTTCTTATTTGTATATTTTTCCTATTTTTCCAAAACATAATGTTATCTACC
TATTATAAGGAGGAACAATT

Downstream 100 bases:

>100_bases
ACGAAAAAAGATAGCTGAAAACAATTTGTCTTCAGCTATCTTTTTTATTTTTGTATCTCTCTATAAAAAGAAAAGTCATT
CATAGTTGGTTGTACTTGTT

Product: pyruvate oxidase

Products: acetyl phosphate; CO2; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MFGKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIKFIQVRHEEAGALAAAAYAKLTGKLGVCMAIAGPGA
IHLLNGLYDAKLDKAPVLAISGQVETDLLGTDFFQEVNLERLFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLT
IPDDVPKFEVKNGARVTNTSFTLPELFPQKNDLDAAKLAINEAKRPVILAGKGTKHARESLLALAEHIGAPIVLTLPAKG
IIPDEHPLCIGGLGLIGTKPSYEAMKHADTLIMVGTSYPFTGFLPEKAKTIHIDTDPAQIGKRYATDIGLAGDADKTLRW
LIENVEEHTDHSFLEHHQEMMKKWEEKLHDQEEDSSIPIKPQRVMHALQQVAHDDAILSVDVGNVTVWTARHFRMTNQQF
VISSWLATLGCGLPGALAGKIAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIVVVVLNNNKIAMIKFEQEVMGNIEFE
TDLTNPDFAKYAEACGGIGYRVENLDELLPAFENAVKQNKPCIIDVVVDANEAPMPAKITFGQAAGYTKHMIKELFEEGK
IDLPPL

Sequences:

>Translated_566_residues
MFGKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIKFIQVRHEEAGALAAAAYAKLTGKLGVCMAIAGPGA
IHLLNGLYDAKLDKAPVLAISGQVETDLLGTDFFQEVNLERLFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLT
IPDDVPKFEVKNGARVTNTSFTLPELFPQKNDLDAAKLAINEAKRPVILAGKGTKHARESLLALAEHIGAPIVLTLPAKG
IIPDEHPLCIGGLGLIGTKPSYEAMKHADTLIMVGTSYPFTGFLPEKAKTIHIDTDPAQIGKRYATDIGLAGDADKTLRW
LIENVEEHTDHSFLEHHQEMMKKWEEKLHDQEEDSSIPIKPQRVMHALQQVAHDDAILSVDVGNVTVWTARHFRMTNQQF
VISSWLATLGCGLPGALAGKIAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIVVVVLNNNKIAMIKFEQEVMGNIEFE
TDLTNPDFAKYAEACGGIGYRVENLDELLPAFENAVKQNKPCIIDVVVDANEAPMPAKITFGQAAGYTKHMIKELFEEGK
IDLPPL
>Mature_566_residues
MFGKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIKFIQVRHEEAGALAAAAYAKLTGKLGVCMAIAGPGA
IHLLNGLYDAKLDKAPVLAISGQVETDLLGTDFFQEVNLERLFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLT
IPDDVPKFEVKNGARVTNTSFTLPELFPQKNDLDAAKLAINEAKRPVILAGKGTKHARESLLALAEHIGAPIVLTLPAKG
IIPDEHPLCIGGLGLIGTKPSYEAMKHADTLIMVGTSYPFTGFLPEKAKTIHIDTDPAQIGKRYATDIGLAGDADKTLRW
LIENVEEHTDHSFLEHHQEMMKKWEEKLHDQEEDSSIPIKPQRVMHALQQVAHDDAILSVDVGNVTVWTARHFRMTNQQF
VISSWLATLGCGLPGALAGKIAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIVVVVLNNNKIAMIKFEQEVMGNIEFE
TDLTNPDFAKYAEACGGIGYRVENLDELLPAFENAVKQNKPCIIDVVVDANEAPMPAKITFGQAAGYTKHMIKELFEEGK
IDLPPL

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=552, Percent_Identity=27.1739130434783, Blast_Score=186, Evalue=7e-47,
Organism=Homo sapiens, GI21361361, Length=587, Percent_Identity=24.190800681431, Blast_Score=134, Evalue=3e-31,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=35.1351351351351, Blast_Score=349, Evalue=3e-97,
Organism=Escherichia coli, GI87081685, Length=560, Percent_Identity=30.3571428571429, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1790104, Length=548, Percent_Identity=30.2919708029197, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1786717, Length=555, Percent_Identity=26.3063063063063, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1788716, Length=551, Percent_Identity=24.8638838475499, Blast_Score=128, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17531299, Length=549, Percent_Identity=24.2258652094718, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=549, Percent_Identity=24.2258652094718, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=568, Percent_Identity=25, Blast_Score=129, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=567, Percent_Identity=29.1005291005291, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6320816, Length=558, Percent_Identity=24.3727598566308, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19922626, Length=569, Percent_Identity=25.8347978910369, Blast_Score=171, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.3.3

Molecular weight: Translated: 61911; Mature: 61911

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIKFIQVRHEEAGALAAA
CCCCHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEECHHHCHHHHH
AYAKLTGKLGVCMAIAGPGAIHLLNGLYDAKLDKAPVLAISGQVETDLLGTDFFQEVNLE
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHCHHHHHHCCHH
RLFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLTIPDDVPKFEVKNGARVTNTS
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCEEECCC
FTLPELFPQKNDLDAAKLAINEAKRPVILAGKGTKHARESLLALAEHIGAPIVLTLPAKG
CCCHHHCCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCC
IIPDEHPLCIGGLGLIGTKPSYEAMKHADTLIMVGTSYPFTGFLPEKAKTIHIDTDPAQI
CCCCCCCEEEECCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHH
GKRYATDIGLAGDADKTLRWLIENVEEHTDHSFLEHHQEMMKKWEEKLHDQEEDSSIPIK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PQRVMHALQQVAHDDAILSVDVGNVTVWTARHFRMTNQQFVISSWLATLGCGLPGALAGK
HHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCHHCCC
IAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIVVVVLNNNKIAMIKFEQEVMGNIEFE
EECCCCEEEEEECCCCCCCCHHHHHHHHEEECCEEEEEECCCEEEEEEECHHHHCCEEEE
TDLTNPDFAKYAEACGGIGYRVENLDELLPAFENAVKQNKPCIIDVVVDANEAPMPAKIT
ECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEE
FGQAAGYTKHMIKELFEEGKIDLPPL
CCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MFGKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIKFIQVRHEEAGALAAA
CCCCHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEECHHHCHHHHH
AYAKLTGKLGVCMAIAGPGAIHLLNGLYDAKLDKAPVLAISGQVETDLLGTDFFQEVNLE
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHCHHHHHHCCHH
RLFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLTIPDDVPKFEVKNGARVTNTS
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCEEECCC
FTLPELFPQKNDLDAAKLAINEAKRPVILAGKGTKHARESLLALAEHIGAPIVLTLPAKG
CCCHHHCCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCC
IIPDEHPLCIGGLGLIGTKPSYEAMKHADTLIMVGTSYPFTGFLPEKAKTIHIDTDPAQI
CCCCCCCEEEECCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHH
GKRYATDIGLAGDADKTLRWLIENVEEHTDHSFLEHHQEMMKKWEEKLHDQEEDSSIPIK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PQRVMHALQQVAHDDAILSVDVGNVTVWTARHFRMTNQQFVISSWLATLGCGLPGALAGK
HHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCHHCCC
IAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIVVVVLNNNKIAMIKFEQEVMGNIEFE
EECCCCEEEEEECCCCCCCCHHHHHHHHEEECCEEEEEECCCEEEEEEECHHHHCCEEEE
TDLTNPDFAKYAEACGGIGYRVENLDELLPAFENAVKQNKPCIIDVVVDANEAPMPAKIT
ECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEE
FGQAAGYTKHMIKELFEEGKIDLPPL
CCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; phosphate; O2; H2O

Specific reaction: pyruvate + phosphate + O2 + H2O = acetyl phosphate + CO2 + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]