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The map label for this gene is parE [H]

Identifier: 218235058

GI number: 218235058

Start: 3626478

End: 3628442

Strand: Reverse

Name: parE [H]

Synonym: BCB4264_A3706

Alternate gene names: 218235058

Gene position: 3628442-3626478 (Counterclockwise)

Preceding gene: 218231714

Following gene: 218234483

Centisome position: 66.96

GC content: 38.83

Gene sequence:

>1965_bases
TTGGCGAAGCATCAGTTCCAATATAATGAAGATGCGATTCAAGTGTTAGAAGGACTTGAAGCCGTTCGAAAACGCCCGGG
TATGTATATCGGGAGTACGGATAGCCGTGGATTGCATCATTTAGTATATGAAATAGTAGATAACTCCGTTGACGAAGCGT
TAGCGGGATTTGGCGACGAAATTTCAGTCGTAATACATAAAGATAATAGTATTAGCGTTATAGATAAAGGGCGAGGAATG
CCTACGGGAATGCATAAACTTGGGAAACCTACACCTGAAGTTATTTTAACTGTACTTCATGCAGGTGGTAAGTTTGGTCA
AGGTGGCTATAAAACGAGTGGTGGTTTACACGGTGTTGGGGCATCAGTTGTAAACGCTTTGTCAGAATGGTTAGTAGTAA
CAATTAAGCGTGATGGTAACATTTATGAACAACGTTTTGAAAATGGTGGCGTTCCTGCAACAACGTTAGAGAAAATTGGG
AAAACAAAAGAGTCTGGTACGACAATGCACTTCAAACCAGATACAACGATTTTCAGCACGACAAATTACAATTATGAAAC
ATTATGTGAGAGATTACGTGAATCTGCATTCTTATTAAAAGGAATGAAAATCTCGATAAAAGACGAACGTAATGATTTAG
AAGATATATTTCATTATGAAACAGGAATCGAAGCTTTCGTTTCATATTTAAACGAAGAAAAAGATTCAATTCACCCGGTT
GTATATTTCACTGGTGAACAAAACGGGATTGAAGCAGAACTAGCATTCCAGTTTAATGATGGTTACTCAGAAAATATTCT
TTCGTTTGTAAATAACGTACGTACAAAAGATGGTGGAACACACGAAGCTGGATTCAAAACAGCGATGACGCGTGTGTTTA
ACGAGTATGCTCGTAAAGTAGCACTATTAAAAGAAAAAGATAAAAACTTAGAAGGTACAGATATTCGTGAAGGTGTAGCG
GCTATCGTTTCGGTACGTGTACCAGAAGAAGTGCTTCAGTTTGAAGGGCAAACGAAGGGCAAACTAGGTACAAGTGAAGC
GCGTTCTTCAATTGATGCCATTGTATCTGAGCATTTAGCATACTTTTTAGAAGAAAACCCTGATGTAGCTACACTTCTTG
TGAGAAAAGCAATTAAAGCAGCACAAGCCCGTGAAGCAGCCCGTAAAGCAAGAGAAGAAGCGCGTAGTGGTAAGAAAAAG
AAAAAATCAGAAGGTACGTTAAGCGGGAAATTAACACCTGCACAATCACGTAACCCGCAAAAAAATGAACTGTACCTAGT
AGAGGGTGACTCTGCCGGTGGTTCTGCGAAACAAGGACGAGACCGACGTTTCCAGGCCGTATTACCGTTACGTGGTAAAG
TAATTAATACAGAAAAAGCAAAGCTTGCTGATATCTTTAAAAATGAAGAGATTAATACAATCATTTATGCGATTGGCGGC
GGTGTAGGAAATGAATTTGATGTTGAAGATATTAACTACGATAAAGTTGTAATTATGACCGATGCCGATACGGATGGGGC
TCATATTCAAGTATTGTTATTAACGTTCTTCTACAGATATATGAAGCCACTTATCGAAGCTGGTAAAGTATTTATCGCAC
TTCCTCCTTTATACAAAGTAAGTAAAGGAAAAGGTAAGAGTGAGGTAATTGAATACGCTTGGTCAGATGAGGAATTAGAC
GGTGTAACGAAAAAAGTTGGAAAAGGCTATATGTTACAACGTTATAAAGGACTTGGTGAAATGAATGCGGATCAATTATG
GGAAACAACAATGAATCCTGAAACGCGTACATTAATTCGCGTGAAAATTGATGATGCAGCAAGAGCAGAGCGTCGCGTTA
CAACATTAATGGGCGATAAAGTAGAACCGCGCCGTAAATGGATTGAACGTAATGTGCAGTTTGGTATGCAAGAAGAAGGA
AATATTTTAGAAAATGAAATGATTATGGAGACGGAGGTGGAATAA

Upstream 100 bases:

>100_bases
ATATAAAAGAGATAAGATAACTTGCCAAAACGATAGTCGAGGCGTTATTCTATAATAGAAACATATGTTCTGATTTTGGA
TTAGGAAAGGGGCAAGGAGT

Downstream 100 bases:

>100_bases
CATGCAAGCAGAGAAGTTTCATGACCTCCCGCTTGAAGACGTGTTAGGTGATCGCTTTGCACGTTATAGTAAATATATTA
TTCAAGATCGTGCACTTCCA

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 654; Mature: 653

Protein sequence:

>654_residues
MAKHQFQYNEDAIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVYEIVDNSVDEALAGFGDEISVVIHKDNSISVIDKGRGM
PTGMHKLGKPTPEVILTVLHAGGKFGQGGYKTSGGLHGVGASVVNALSEWLVVTIKRDGNIYEQRFENGGVPATTLEKIG
KTKESGTTMHFKPDTTIFSTTNYNYETLCERLRESAFLLKGMKISIKDERNDLEDIFHYETGIEAFVSYLNEEKDSIHPV
VYFTGEQNGIEAELAFQFNDGYSENILSFVNNVRTKDGGTHEAGFKTAMTRVFNEYARKVALLKEKDKNLEGTDIREGVA
AIVSVRVPEEVLQFEGQTKGKLGTSEARSSIDAIVSEHLAYFLEENPDVATLLVRKAIKAAQAREAARKAREEARSGKKK
KKSEGTLSGKLTPAQSRNPQKNELYLVEGDSAGGSAKQGRDRRFQAVLPLRGKVINTEKAKLADIFKNEEINTIIYAIGG
GVGNEFDVEDINYDKVVIMTDADTDGAHIQVLLLTFFYRYMKPLIEAGKVFIALPPLYKVSKGKGKSEVIEYAWSDEELD
GVTKKVGKGYMLQRYKGLGEMNADQLWETTMNPETRTLIRVKIDDAARAERRVTTLMGDKVEPRRKWIERNVQFGMQEEG
NILENEMIMETEVE

Sequences:

>Translated_654_residues
MAKHQFQYNEDAIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVYEIVDNSVDEALAGFGDEISVVIHKDNSISVIDKGRGM
PTGMHKLGKPTPEVILTVLHAGGKFGQGGYKTSGGLHGVGASVVNALSEWLVVTIKRDGNIYEQRFENGGVPATTLEKIG
KTKESGTTMHFKPDTTIFSTTNYNYETLCERLRESAFLLKGMKISIKDERNDLEDIFHYETGIEAFVSYLNEEKDSIHPV
VYFTGEQNGIEAELAFQFNDGYSENILSFVNNVRTKDGGTHEAGFKTAMTRVFNEYARKVALLKEKDKNLEGTDIREGVA
AIVSVRVPEEVLQFEGQTKGKLGTSEARSSIDAIVSEHLAYFLEENPDVATLLVRKAIKAAQAREAARKAREEARSGKKK
KKSEGTLSGKLTPAQSRNPQKNELYLVEGDSAGGSAKQGRDRRFQAVLPLRGKVINTEKAKLADIFKNEEINTIIYAIGG
GVGNEFDVEDINYDKVVIMTDADTDGAHIQVLLLTFFYRYMKPLIEAGKVFIALPPLYKVSKGKGKSEVIEYAWSDEELD
GVTKKVGKGYMLQRYKGLGEMNADQLWETTMNPETRTLIRVKIDDAARAERRVTTLMGDKVEPRRKWIERNVQFGMQEEG
NILENEMIMETEVE
>Mature_653_residues
AKHQFQYNEDAIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVYEIVDNSVDEALAGFGDEISVVIHKDNSISVIDKGRGMP
TGMHKLGKPTPEVILTVLHAGGKFGQGGYKTSGGLHGVGASVVNALSEWLVVTIKRDGNIYEQRFENGGVPATTLEKIGK
TKESGTTMHFKPDTTIFSTTNYNYETLCERLRESAFLLKGMKISIKDERNDLEDIFHYETGIEAFVSYLNEEKDSIHPVV
YFTGEQNGIEAELAFQFNDGYSENILSFVNNVRTKDGGTHEAGFKTAMTRVFNEYARKVALLKEKDKNLEGTDIREGVAA
IVSVRVPEEVLQFEGQTKGKLGTSEARSSIDAIVSEHLAYFLEENPDVATLLVRKAIKAAQAREAARKAREEARSGKKKK
KSEGTLSGKLTPAQSRNPQKNELYLVEGDSAGGSAKQGRDRRFQAVLPLRGKVINTEKAKLADIFKNEEINTIIYAIGGG
VGNEFDVEDINYDKVVIMTDADTDGAHIQVLLLTFFYRYMKPLIEAGKVFIALPPLYKVSKGKGKSEVIEYAWSDEELDG
VTKKVGKGYMLQRYKGLGEMNADQLWETTMNPETRTLIRVKIDDAARAERRVTTLMGDKVEPRRKWIERNVQFGMQEEGN
ILENEMIMETEVE

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=630, Percent_Identity=25.8730158730159, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI19913408, Length=661, Percent_Identity=24.0544629349471, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI48994957, Length=558, Percent_Identity=52.3297491039427, Blast_Score=537, Evalue=1e-153,
Organism=Escherichia coli, GI1789408, Length=656, Percent_Identity=38.8719512195122, Blast_Score=424, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17535065, Length=679, Percent_Identity=24.8895434462445, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=600, Percent_Identity=25.1666666666667, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI212645657, Length=248, Percent_Identity=27.4193548387097, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=655, Percent_Identity=27.3282442748092, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17136538, Length=675, Percent_Identity=24.8888888888889, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005740 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 72910; Mature: 72778

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHQFQYNEDAIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVYEIVDNSVDEALAGFGDE
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHCCCCC
ISVVIHKDNSISVIDKGRGMPTGMHKLGKPTPEVILTVLHAGGKFGQGGYKTSGGLHGVG
EEEEEECCCCEEEEECCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
ASVVNALSEWLVVTIKRDGNIYEQRFENGGVPATTLEKIGKTKESGTTMHFKPDTTIFST
HHHHHHHHHHEEEEEECCCCHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEECCCCEEEEC
TNYNYETLCERLRESAFLLKGMKISIKDERNDLEDIFHYETGIEAFVSYLNEEKDSIHPV
CCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
VYFTGEQNGIEAELAFQFNDGYSENILSFVNNVRTKDGGTHEAGFKTAMTRVFNEYARKV
EEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
ALLKEKDKNLEGTDIREGVAAIVSVRVPEEVLQFEGQTKGKLGTSEARSSIDAIVSEHLA
HHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
YFLEENPDVATLLVRKAIKAAQAREAARKAREEARSGKKKKKSEGTLSGKLTPAQSRNPQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCC
KNELYLVEGDSAGGSAKQGRDRRFQAVLPLRGKVINTEKAKLADIFKNEEINTIIYAIGG
CCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHHHHCCCCCCEEEEEECC
GVGNEFDVEDINYDKVVIMTDADTDGAHIQVLLLTFFYRYMKPLIEAGKVFIALPPLYKV
CCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
SKGKGKSEVIEYAWSDEELDGVTKKVGKGYMLQRYKGLGEMNADQLWETTMNPETRTLIR
CCCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEE
VKIDDAARAERRVTTLMGDKVEPRRKWIERNVQFGMQEEGNILENEMIMETEVE
EEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHCCEEEEECCC
>Mature Secondary Structure 
AKHQFQYNEDAIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVYEIVDNSVDEALAGFGDE
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHCCCCC
ISVVIHKDNSISVIDKGRGMPTGMHKLGKPTPEVILTVLHAGGKFGQGGYKTSGGLHGVG
EEEEEECCCCEEEEECCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
ASVVNALSEWLVVTIKRDGNIYEQRFENGGVPATTLEKIGKTKESGTTMHFKPDTTIFST
HHHHHHHHHHEEEEEECCCCHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEECCCCEEEEC
TNYNYETLCERLRESAFLLKGMKISIKDERNDLEDIFHYETGIEAFVSYLNEEKDSIHPV
CCCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
VYFTGEQNGIEAELAFQFNDGYSENILSFVNNVRTKDGGTHEAGFKTAMTRVFNEYARKV
EEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
ALLKEKDKNLEGTDIREGVAAIVSVRVPEEVLQFEGQTKGKLGTSEARSSIDAIVSEHLA
HHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
YFLEENPDVATLLVRKAIKAAQAREAARKAREEARSGKKKKKSEGTLSGKLTPAQSRNPQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCC
KNELYLVEGDSAGGSAKQGRDRRFQAVLPLRGKVINTEKAKLADIFKNEEINTIIYAIGG
CCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHHHHCCCCCCEEEEEECC
GVGNEFDVEDINYDKVVIMTDADTDGAHIQVLLLTFFYRYMKPLIEAGKVFIALPPLYKV
CCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
SKGKGKSEVIEYAWSDEELDGVTKKVGKGYMLQRYKGLGEMNADQLWETTMNPETRTLIR
CCCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEE
VKIDDAARAERRVTTLMGDKVEPRRKWIERNVQFGMQEEGNILENEMIMETEVE
EEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969507; 9539793; 9384377 [H]