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The map label for this gene is hisF [H]

Identifier: 218235029

GI number: 218235029

Start: 1404713

End: 1405471

Strand: Direct

Name: hisF [H]

Synonym: BCB4264_A1463

Alternate gene names: 218235029

Gene position: 1404713-1405471 (Clockwise)

Preceding gene: 218231891

Following gene: 218235744

Centisome position: 25.92

GC content: 40.58

Gene sequence:

>759_bases
ATGTTAGCGAAACGAATTATTCCATGTCTAGATGTGAAAGAAGGGCGAGTCGTAAAGGGTGTAAATTTTATAGGATTACA
AGATGTCGGTGATCCGGTTGAAATAGCTGAAGCATATAACGTGGCAGGGGCAGATGAAATCGTATTTTTAGATATTACAG
CGACTCATGAAGGAAGAAAAACGATTATAGATGTTGTAGAAAAAACAGCAGCAAAAGTATTTATTCCTCTTACGGTTGGC
GGGGGGATATCAAGTGTGAAAGATATGTACAATTTACTAAGAGCTGGGGCAGACAAAGTTTCAATAAACTCAGCGGCAGT
GCGAAACCCGAAGTTAATAGAAGAAGGGGCAGAACACTTTGGATCACAATGTATTGTCGTGGCAATTGATGCTAGAAAAG
TAGCAGAAGATAAATGGAACGTATACGTCAACGGCGGAAGAGTTGACACAGGAATGGATGCCATAGAATGGGCGAATCGT
GTTGTTAAACTTGGGGCAGGTGAAATTTTGTTAACGAGTATGGATGCAGATGGAACGAAAAGCGGATATGACCTTCGTTT
AACAGAAGAAATTTCAAAAAGTGTTTCTATTCCAGTCATCGCATCAGGGGGCTGTGGTCATACGGATCATATTATAGAAG
TTTTTCAAAAGACAACAGTTGATGCAGCGTTAGCGGCATCCATCTTTCATTATGGTGAGGCGACAGTGCATAGTGTAAAG
CGAAAATTAAGAGATGCAAATATTGAGGTGCGGTTATGA

Upstream 100 bases:

>100_bases
TGAAAAAGTTAAATGATATGAATATATATGGTGTCATAATTGGAAAGGCGCTTTACGAGAAAACGATTGATTTAGAAGAA
GTGTTACAGGTAACGAAGTT

Downstream 100 bases:

>100_bases
AACCTAATTTTTCAAAAGGATTAATACCGGCAATTGTCATCGAAGAAGGTACGAAAGACGTTTTAATGCTAGCTTATATG
AATGAAGAAGCATATGAAAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAEAYNVAGADEIVFLDITATHEGRKTIIDVVEKTAAKVFIPLTVG
GGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAEHFGSQCIVVAIDARKVAEDKWNVYVNGGRVDTGMDAIEWANR
VVKLGAGEILLTSMDADGTKSGYDLRLTEEISKSVSIPVIASGGCGHTDHIIEVFQKTTVDAALAASIFHYGEATVHSVK
RKLRDANIEVRL

Sequences:

>Translated_252_residues
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAEAYNVAGADEIVFLDITATHEGRKTIIDVVEKTAAKVFIPLTVG
GGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAEHFGSQCIVVAIDARKVAEDKWNVYVNGGRVDTGMDAIEWANR
VVKLGAGEILLTSMDADGTKSGYDLRLTEEISKSVSIPVIASGGCGHTDHIIEVFQKTTVDAALAASIFHYGEATVHSVK
RKLRDANIEVRL
>Mature_252_residues
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAEAYNVAGADEIVFLDITATHEGRKTIIDVVEKTAAKVFIPLTVG
GGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAEHFGSQCIVVAIDARKVAEDKWNVYVNGGRVDTGMDAIEWANR
VVKLGAGEILLTSMDADGTKSGYDLRLTEEISKSVSIPVIASGGCGHTDHIIEVFQKTTVDAALAASIFHYGEATVHSVK
RKLRDANIEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=44.9612403100775, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87082028, Length=210, Percent_Identity=29.5238095238095, Blast_Score=93, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=34.7133757961783, Blast_Score=160, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27028; Mature: 27028

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAEAYNVAGADEIVFLDITATHEGRK
CCHHHCCCCCCCCCCCEEECEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCHH
TIIDVVEKTAAKVFIPLTVGGGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAEHF
HHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCCHHHHHHHHHH
GSQCIVVAIDARKVAEDKWNVYVNGGRVDTGMDAIEWANRVVKLGAGEILLTSMDADGTK
CCCEEEEEECCHHHCCCCCEEEEECCEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCC
SGYDLRLTEEISKSVSIPVIASGGCGHTDHIIEVFQKTTVDAALAASIFHYGEATVHSVK
CCCEEEEHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RKLRDANIEVRL
HHHHCCCEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAEAYNVAGADEIVFLDITATHEGRK
CCHHHCCCCCCCCCCCEEECEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCHH
TIIDVVEKTAAKVFIPLTVGGGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAEHF
HHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCCHHHHHHHHHH
GSQCIVVAIDARKVAEDKWNVYVNGGRVDTGMDAIEWANRVVKLGAGEILLTSMDADGTK
CCCEEEEEECCHHHCCCCCEEEEECCEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCC
SGYDLRLTEEISKSVSIPVIASGGCGHTDHIIEVFQKTTVDAALAASIFHYGEATVHSVK
CCCEEEEHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RKLRDANIEVRL
HHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA