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The map label for this gene is ytsJ [H]

Identifier: 218235005

GI number: 218235005

Start: 3010691

End: 3011935

Strand: Reverse

Name: ytsJ [H]

Synonym: BCB4264_A3134

Alternate gene names: 218235005

Gene position: 3011935-3010691 (Counterclockwise)

Preceding gene: 218235738

Following gene: 218231095

Centisome position: 55.58

GC content: 36.06

Gene sequence:

>1245_bases
ATGTCACCTTTACGAGATGAAGCATTAAAAATGCATAAAGAAAAACAGGGGAAGATTGGTGTTTATTCTAAAGTTAAAGT
ACAAAATTCAACAGATTTAAGTCTCGCTTACTCACCAGGTGTTGCTGAGCCATGCTTAGAGATTTATAAAGATGAAAGTA
AAGTATATGATTATACAATGAAAGGGAACCTAGTTGGAGTTATATCTGATGGAACTGCAGTATTAGGTCTTGGGAATATT
GGACCTAAAGCAGCTATGCCAGTGATGGAGGGAAAAGCTTTACTTTTCAAATCATTTGCTGAGATCGATGCTTTTCCTAT
TTGTTTAAACACTAATAATCCGGATAAGATTGTTGAGGTTGTTAAATTATTAGAACTAAATTTTGGTGGGATAAACTTAG
AAGATATTGCAGCACCTCAATGCTTTGAAATTGAGGAGCGCCTAAGAAAATCTTGTGATATTCCTGTTTTCCATGATGAT
CAACACGGTACAGCTATTGTAACGATTGCTGGCCTAATTAATGCACTTAAGTTAACAAATAAAAAAATTGAAGATGTTCA
AATCGTTGTAAATGGCGCTGGTGCTGCTGGGATAGCCATTGTTAAGTTGTTACTCCATATGCATGTGAAAAATGTTATTT
TGTGTGACACAAAAGGGATAGTATATGAACAGCGACCTATTGGAATGAATGCGCTTAAAGAAGAAATAGCTCGTATTACA
AATATAGAACAGAAGCGGGGAAGATTAGCAGAGGCCCTGATTGATGCTGATGTATTTATTGGCGTATCAGCTGCAGGTGT
AGTTGATGAGGATATGGTTCGTTCTATGAATCATAATCCTATTATTTTTGCCTTGGCTAACCCTACACCTGAAATTATGC
CTAATAAAGCAAAAATAGCAGGAGCACTAGTAGTTGGTACAGGACGTTCTGACTTTCCTAATCAAGTAAATAATGTATTA
GCTTTCCCTGGAGTTTTTCGTGGAGCCTTAGATGTACAAGCAAAAGAAATTAATGAAGAAATGAAGCTAGCAGCTGTTTA
TGCAATAGCTGATTTAATTTCAGAAGAAGAGCTTCATGTCAACTATGTCATTCCAAATCCGTTTGATCCTAGGGTGGTGC
CACATGTAGCATATGCTGTCGCACGTGCTGCAGTAGAAACCGGTGTTTCAAGAAAAAATATCAGCATGTCTGAAATTAAA
AACAATCTATTCAGGTTAACAAAAGAGACAAATATATTAAAATAA

Upstream 100 bases:

>100_bases
AATAAATAATGATTATATTAGAAGTCTTACTTATATCATACAATATCAAGTTTAAGATAGAGTTAATGAACTCTTAACTT
AATTATAGGAGCGAAATTAT

Downstream 100 bases:

>100_bases
ATATTTTTCTTAAGAAAATATTATGTAGTAAAGGTTATCTACTTAGTTCTTAGCGTATAAAAAATTAAATTATGTATACC
TCCCTTTCTGATATTGGTAA

Product: putative malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MSPLRDEALKMHKEKQGKIGVYSKVKVQNSTDLSLAYSPGVAEPCLEIYKDESKVYDYTMKGNLVGVISDGTAVLGLGNI
GPKAAMPVMEGKALLFKSFAEIDAFPICLNTNNPDKIVEVVKLLELNFGGINLEDIAAPQCFEIEERLRKSCDIPVFHDD
QHGTAIVTIAGLINALKLTNKKIEDVQIVVNGAGAAGIAIVKLLLHMHVKNVILCDTKGIVYEQRPIGMNALKEEIARIT
NIEQKRGRLAEALIDADVFIGVSAAGVVDEDMVRSMNHNPIIFALANPTPEIMPNKAKIAGALVVGTGRSDFPNQVNNVL
AFPGVFRGALDVQAKEINEEMKLAAVYAIADLISEEELHVNYVIPNPFDPRVVPHVAYAVARAAVETGVSRKNISMSEIK
NNLFRLTKETNILK

Sequences:

>Translated_414_residues
MSPLRDEALKMHKEKQGKIGVYSKVKVQNSTDLSLAYSPGVAEPCLEIYKDESKVYDYTMKGNLVGVISDGTAVLGLGNI
GPKAAMPVMEGKALLFKSFAEIDAFPICLNTNNPDKIVEVVKLLELNFGGINLEDIAAPQCFEIEERLRKSCDIPVFHDD
QHGTAIVTIAGLINALKLTNKKIEDVQIVVNGAGAAGIAIVKLLLHMHVKNVILCDTKGIVYEQRPIGMNALKEEIARIT
NIEQKRGRLAEALIDADVFIGVSAAGVVDEDMVRSMNHNPIIFALANPTPEIMPNKAKIAGALVVGTGRSDFPNQVNNVL
AFPGVFRGALDVQAKEINEEMKLAAVYAIADLISEEELHVNYVIPNPFDPRVVPHVAYAVARAAVETGVSRKNISMSEIK
NNLFRLTKETNILK
>Mature_413_residues
SPLRDEALKMHKEKQGKIGVYSKVKVQNSTDLSLAYSPGVAEPCLEIYKDESKVYDYTMKGNLVGVISDGTAVLGLGNIG
PKAAMPVMEGKALLFKSFAEIDAFPICLNTNNPDKIVEVVKLLELNFGGINLEDIAAPQCFEIEERLRKSCDIPVFHDDQ
HGTAIVTIAGLINALKLTNKKIEDVQIVVNGAGAAGIAIVKLLLHMHVKNVILCDTKGIVYEQRPIGMNALKEEIARITN
IEQKRGRLAEALIDADVFIGVSAAGVVDEDMVRSMNHNPIIFALANPTPEIMPNKAKIAGALVVGTGRSDFPNQVNNVLA
FPGVFRGALDVQAKEINEEMKLAAVYAIADLISEEELHVNYVIPNPFDPRVVPHVAYAVARAAVETGVSRKNISMSEIKN
NLFRLTKETNILK

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=362, Percent_Identity=27.0718232044199, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI62420882, Length=363, Percent_Identity=27.5482093663912, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI62420880, Length=363, Percent_Identity=27.5482093663912, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI239049447, Length=363, Percent_Identity=27.5482093663912, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI4505145, Length=361, Percent_Identity=26.8698060941828, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI270265879, Length=320, Percent_Identity=27.5, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788806, Length=429, Percent_Identity=49.6503496503497, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI87081919, Length=456, Percent_Identity=25.6578947368421, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17537199, Length=424, Percent_Identity=26.4150943396226, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6322823, Length=345, Percent_Identity=28.1159420289855, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24646388, Length=380, Percent_Identity=27.8947368421053, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24646386, Length=380, Percent_Identity=27.8947368421053, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI78707242, Length=413, Percent_Identity=26.1501210653753, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI78707238, Length=413, Percent_Identity=26.1501210653753, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356279, Length=477, Percent_Identity=24.9475890985325, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281362672, Length=477, Percent_Identity=24.9475890985325, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281362674, Length=477, Percent_Identity=24.9475890985325, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI78707240, Length=361, Percent_Identity=27.1468144044321, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI78707232, Length=390, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281363505, Length=359, Percent_Identity=27.0194986072423, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI78707236, Length=359, Percent_Identity=27.0194986072423, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281363503, Length=359, Percent_Identity=27.0194986072423, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 45045; Mature: 44914

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLRDEALKMHKEKQGKIGVYSKVKVQNSTDLSLAYSPGVAEPCLEIYKDESKVYDYTM
CCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEE
KGNLVGVISDGTAVLGLGNIGPKAAMPVMEGKALLFKSFAEIDAFPICLNTNNPDKIVEV
CCCEEEEEECCCEEEEECCCCCHHCCCHHCCHHHHHHHHHHCCCEEEEEECCCHHHHHHH
VKLLELNFGGINLEDIAAPQCFEIEERLRKSCDIPVFHDDQHGTAIVTIAGLINALKLTN
HHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHHCC
KKIEDVQIVVNGAGAAGIAIVKLLLHMHVKNVILCDTKGIVYEQRPIGMNALKEEIARIT
CCCCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCEEECCCCCHHHHHHHHHHHH
NIEQKRGRLAEALIDADVFIGVSAAGVVDEDMVRSMNHNPIIFALANPTPEIMPNKAKIA
CCHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCHHEE
GALVVGTGRSDFPNQVNNVLAFPGVFRGALDVQAKEINEEMKLAAVYAIADLISEEELHV
EEEEEECCCCCCCHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
NYVIPNPFDPRVVPHVAYAVARAAVETGVSRKNISMSEIKNNLFRLTKETNILK
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SPLRDEALKMHKEKQGKIGVYSKVKVQNSTDLSLAYSPGVAEPCLEIYKDESKVYDYTM
CCHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEE
KGNLVGVISDGTAVLGLGNIGPKAAMPVMEGKALLFKSFAEIDAFPICLNTNNPDKIVEV
CCCEEEEEECCCEEEEECCCCCHHCCCHHCCHHHHHHHHHHCCCEEEEEECCCHHHHHHH
VKLLELNFGGINLEDIAAPQCFEIEERLRKSCDIPVFHDDQHGTAIVTIAGLINALKLTN
HHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHHCC
KKIEDVQIVVNGAGAAGIAIVKLLLHMHVKNVILCDTKGIVYEQRPIGMNALKEEIARIT
CCCCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCEEECCCCCHHHHHHHHHHHH
NIEQKRGRLAEALIDADVFIGVSAAGVVDEDMVRSMNHNPIIFALANPTPEIMPNKAKIA
CCHHHHHHHHHHHHCCHHEEEECCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCHHEE
GALVVGTGRSDFPNQVNNVLAFPGVFRGALDVQAKEINEEMKLAAVYAIADLISEEELHV
EEEEEECCCCCCCHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
NYVIPNPFDPRVVPHVAYAVARAAVETGVSRKNISMSEIKNNLFRLTKETNILK
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]