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The map label for this gene is fsR [H]

Identifier: 218235000

GI number: 218235000

Start: 1742366

End: 1743577

Strand: Direct

Name: fsR [H]

Synonym: BCB4264_A1836

Alternate gene names: 218235000

Gene position: 1742366-1743577 (Clockwise)

Preceding gene: 218235676

Following gene: 218234233

Centisome position: 32.15

GC content: 38.37

Gene sequence:

>1212_bases
ATGCAGGCAGTTTCGCAAAAAAACACAGTAGAAACACCGACAATATATCGAATATTATTTGCGATTAGCTTTGGACATTT
TTTAAATGATTCGATGCAAGCAGTTGTGCCGGCGTTGTTTCCTATTTTGGAAAAAACGATGAATTTATCCTATATGCAAG
TAGGGTGGATTGCGTTTGCGTTAAATATGACGTCATCGATTATGCAACCGGTGTTTGGTATGTATTCAGATAAGAAGCCG
TCACCATTTTTATTACCACTCGGCATGTTTTCAAGTATGCTTGGAATGATTGGACTCGCGTTTGCACCAAACTTTATTAT
TGTTATTATTTCTGTTTTATTTATTGGTTTAGGTTCCGCAGTCTTTCATCCAGAAGGCGCTCGTGTTGCGTATATGGCGG
CAGGTGCAAAACGAGGGTTAGCGCAAGCGATTTATCAAGTTGGGGGAAACACGGGGAATTCTTTAGCTCCTATTTTTACA
GCGCTAATTTTCGTTCCACTCGGTCAAATTGGTTCATTGGGTTTTACAGCATTTGCAGCAGTAGGAATTGCATTATTAAT
TTTCGTATCGAATTGGTACAAAAATGAATTAGCAACCGGTGCTGTAAGAAGGAAGAAGAGGGCTGCACTTGAGGCGGAAA
ATGCAATTGTAAGTACACACATTAAATTCGTTATTATACTTCTTGTTTTTCTTACTTTTGTACGCTCTTGGTACGGTGCT
GGTATCGGGAATTTCTATCAATTTTACTTAATAGAGCATTACGGTTTATCTATAAAAAATGCCCAGTATTTCGTATTCGC
ATTTATGATTGCTGGTGTGTTAGGCACTTTCTTCGGAGGACCGTTAGCAGATCGATTTGGTAAGAAAAATATCATCGTAT
TTTCAATGTTAGGTTCAGCGCCACTTGCACTGTTATTACCACATGTCTCACTTGTGTGGGTCGTACCGTTATTTTTATGT
ATCGGTTTTATTAGTTCAAGTAGTTTTAGTGTAATTGTTGTATATGCACAAGAGCTTGTGCCTGGAAAAGTGGGAATGGT
GTCAGGATTAATCGTAGGTCTTGCATTTGGACTTGGAGCATTAGGTGCTGTAGTTCTTGGGAAATTAGCAGATGTATATA
GCCTGCAATTTATTATGCTATTATGTAGTTGTCTACCATTAATTGGACTCACTTCATGGTTACTGCCAAGTGATAAGAAA
ACGATAGAATAG

Upstream 100 bases:

>100_bases
AGAAATGAAAGCAGTATAGAATAGAAATGTTTCAAAAGTCAGAATTTATTACACCTACTGTAATAAATATATTTTTTGTA
CAGAAAGGAGCGATTCACAT

Downstream 100 bases:

>100_bases
GGGATGCGCTATGAAATTGTGTGAAAACGTTACGGAATTAATAGGAGATACACCTGTCGTCCGATTATCTAAATTTATTC
CGGAAGACGCAGCAGATGTG

Product: fosmidomycin resistance protein

Products: fosmidomycin [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MQAVSQKNTVETPTIYRILFAISFGHFLNDSMQAVVPALFPILEKTMNLSYMQVGWIAFALNMTSSIMQPVFGMYSDKKP
SPFLLPLGMFSSMLGMIGLAFAPNFIIVIISVLFIGLGSAVFHPEGARVAYMAAGAKRGLAQAIYQVGGNTGNSLAPIFT
ALIFVPLGQIGSLGFTAFAAVGIALLIFVSNWYKNELATGAVRRKKRAALEAENAIVSTHIKFVIILLVFLTFVRSWYGA
GIGNFYQFYLIEHYGLSIKNAQYFVFAFMIAGVLGTFFGGPLADRFGKKNIIVFSMLGSAPLALLLPHVSLVWVVPLFLC
IGFISSSSFSVIVVYAQELVPGKVGMVSGLIVGLAFGLGALGAVVLGKLADVYSLQFIMLLCSCLPLIGLTSWLLPSDKK
TIE

Sequences:

>Translated_403_residues
MQAVSQKNTVETPTIYRILFAISFGHFLNDSMQAVVPALFPILEKTMNLSYMQVGWIAFALNMTSSIMQPVFGMYSDKKP
SPFLLPLGMFSSMLGMIGLAFAPNFIIVIISVLFIGLGSAVFHPEGARVAYMAAGAKRGLAQAIYQVGGNTGNSLAPIFT
ALIFVPLGQIGSLGFTAFAAVGIALLIFVSNWYKNELATGAVRRKKRAALEAENAIVSTHIKFVIILLVFLTFVRSWYGA
GIGNFYQFYLIEHYGLSIKNAQYFVFAFMIAGVLGTFFGGPLADRFGKKNIIVFSMLGSAPLALLLPHVSLVWVVPLFLC
IGFISSSSFSVIVVYAQELVPGKVGMVSGLIVGLAFGLGALGAVVLGKLADVYSLQFIMLLCSCLPLIGLTSWLLPSDKK
TIE
>Mature_403_residues
MQAVSQKNTVETPTIYRILFAISFGHFLNDSMQAVVPALFPILEKTMNLSYMQVGWIAFALNMTSSIMQPVFGMYSDKKP
SPFLLPLGMFSSMLGMIGLAFAPNFIIVIISVLFIGLGSAVFHPEGARVAYMAAGAKRGLAQAIYQVGGNTGNSLAPIFT
ALIFVPLGQIGSLGFTAFAAVGIALLIFVSNWYKNELATGAVRRKKRAALEAENAIVSTHIKFVIILLVFLTFVRSWYGA
GIGNFYQFYLIEHYGLSIKNAQYFVFAFMIAGVLGTFFGGPLADRFGKKNIIVFSMLGSAPLALLLPHVSLVWVVPLFLC
IGFISSSSFSVIVVYAQELVPGKVGMVSGLIVGLAFGLGALGAVVLGKLADVYSLQFIMLLCSCLPLIGLTSWLLPSDKK
TIE

Specific function: Confers The Resistance Against Fosmidomycin. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786686, Length=395, Percent_Identity=42.2784810126582, Blast_Score=296, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43442; Mature: 43442

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAVSQKNTVETPTIYRILFAISFGHFLNDSMQAVVPALFPILEKTMNLSYMQVGWIAFA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LNMTSSIMQPVFGMYSDKKPSPFLLPLGMFSSMLGMIGLAFAPNFIIVIISVLFIGLGSA
HHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCH
VFHPEGARVAYMAAGAKRGLAQAIYQVGGNTGNSLAPIFTALIFVPLGQIGSLGFTAFAA
HCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
VGIALLIFVSNWYKNELATGAVRRKKRAALEAENAIVSTHIKFVIILLVFLTFVRSWYGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GIGNFYQFYLIEHYGLSIKNAQYFVFAFMIAGVLGTFFGGPLADRFGKKNIIVFSMLGSA
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEECCCC
PLALLLPHVSLVWVVPLFLCIGFISSSSFSVIVVYAQELVPGKVGMVSGLIVGLAFGLGA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCHHHHHHHHHHHHHHHHHH
LGAVVLGKLADVYSLQFIMLLCSCLPLIGLTSWLLPSDKKTIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQAVSQKNTVETPTIYRILFAISFGHFLNDSMQAVVPALFPILEKTMNLSYMQVGWIAFA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LNMTSSIMQPVFGMYSDKKPSPFLLPLGMFSSMLGMIGLAFAPNFIIVIISVLFIGLGSA
HHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCH
VFHPEGARVAYMAAGAKRGLAQAIYQVGGNTGNSLAPIFTALIFVPLGQIGSLGFTAFAA
HCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
VGIALLIFVSNWYKNELATGAVRRKKRAALEAENAIVSTHIKFVIILLVFLTFVRSWYGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GIGNFYQFYLIEHYGLSIKNAQYFVFAFMIAGVLGTFFGGPLADRFGKKNIIVFSMLGSA
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEECCCC
PLALLLPHVSLVWVVPLFLCIGFISSSSFSVIVVYAQELVPGKVGMVSGLIVGLAFGLGA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCCHHHHHHHHHHHHHHHHHH
LGAVVLGKLADVYSLQFIMLLCSCLPLIGLTSWLLPSDKKTIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: fosmidomycin [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: fosmidomycin [Cytoplasm] + Proton [Periplasm] = fosmidomycin [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]