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The map label for this gene is thiO [H]

Identifier: 218234998

GI number: 218234998

Start: 765291

End: 766400

Strand: Direct

Name: thiO [H]

Synonym: BCB4264_A0787

Alternate gene names: 218234998

Gene position: 765291-766400 (Clockwise)

Preceding gene: 218235918

Following gene: 218234436

Centisome position: 14.12

GC content: 38.92

Gene sequence:

>1110_bases
ATGTGTGAGAAGTATGATGTAGCGATTATTGGTGGTGGTGTAATTGGTAGTTCAATTGCACATTTTCTAGCAGAAAGAGG
ACATAAAGTAGCGATTGTGGAGAAGCAAAGTATTGCATCGGAAGCTTCAAAAGCAGCTGCTGGTTTACTTGGTGTTCAGG
CAGAATGGGATGCGTATGATCCGCTATTTGAACTTGCTAGAGAAAGCCGAGCTATATTTCCACAACTTGCAGCAGTTTTA
CGCGAAAAAACGGGTGTTGATATTGGATATGAAGAAAAAGGAATTTATCGTATTGCTCAAAATGAAGATGAGAAGGAAAG
AATTCTTCACATTATGGATTGGCAGCAGAAAACAGGTGAAGATTCTTATTTTCTAACGGGAGACCATGTGCGGGAAAAAG
AGCCATATCTATCCGAATCTATTATAGGAGCAGTATATTATCCGAAAGACGGTCATGTTATTGCACCAGAGCTTACGAAA
GCATTCGCACATTCTGCAGCGATTTCCGGTGCTGATATATATGAACAGACAGAAGTATTTGATATCCGTATTGAAAATAA
TAAAGTGACTGGAGTTATTACAAGTGAAGGTATTGTCACATGTGAGAAAGTCGTTATTGCAGGAGGTTCATGGAGCACGA
AGTTACTCAGTTATTTTCACCGCGATTGGGGTACATATCCAGTTAAAGGAGAAGTGGTTGCGGTAAGAAGTAGAAAACAA
CTTTTAAAAGCACCTATTTTTCAAGAAAGGTTTTACATTACTCCAAAGCGTGGTGGACGTTACGTAATTGGGGCAACAAT
GAAGCCACATACGTTCAATAAAACTGTGCAGCCAGAAAGTATAACTTCTATATTAGAGCGTGCTTATACAATATTGCCAG
CTTTAAAAGAAGCAGAATGGGAAAGCACGTGGGCAGGATTAAGACCACAATCGAATCATGAAGCTCCTTATATGGGAGAG
CATGAAGAAATAAAAGGTTTATATGCTTGCACGGGCCATTATCGAAACGGTATTTTATTAAGTCCTATTTCTGGCCAATA
TATGGCTGATTTAATAGAAGGAAAGCAAGAGAATCATTTGCTAGATTCATTGCTTTCTAGAAGAGTTTAG

Upstream 100 bases:

>100_bases
GTAAGCGGTATAGCAGTTATGTCTGGAATTGTAAGTAGTAGTAACCCGTATAGCAAAGCGAAATCTTATAAGGAATCAAT
AAGAAAGTGGGCGGAGAAAC

Downstream 100 bases:

>100_bases
AAAGGGGATGGAAGTTTGAATTTAAAAATTAATGGTAACCAAATTGAAGTGCCAGCGAGTGTAAAAACAGTAGCTGAGCT
ACTTACACATTTAGAGCTAG

Product: glycine oxidase ThiO

Products: NA

Alternate protein names: GO [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MCEKYDVAIIGGGVIGSSIAHFLAERGHKVAIVEKQSIASEASKAAAGLLGVQAEWDAYDPLFELARESRAIFPQLAAVL
REKTGVDIGYEEKGIYRIAQNEDEKERILHIMDWQQKTGEDSYFLTGDHVREKEPYLSESIIGAVYYPKDGHVIAPELTK
AFAHSAAISGADIYEQTEVFDIRIENNKVTGVITSEGIVTCEKVVIAGGSWSTKLLSYFHRDWGTYPVKGEVVAVRSRKQ
LLKAPIFQERFYITPKRGGRYVIGATMKPHTFNKTVQPESITSILERAYTILPALKEAEWESTWAGLRPQSNHEAPYMGE
HEEIKGLYACTGHYRNGILLSPISGQYMADLIEGKQENHLLDSLLSRRV

Sequences:

>Translated_369_residues
MCEKYDVAIIGGGVIGSSIAHFLAERGHKVAIVEKQSIASEASKAAAGLLGVQAEWDAYDPLFELARESRAIFPQLAAVL
REKTGVDIGYEEKGIYRIAQNEDEKERILHIMDWQQKTGEDSYFLTGDHVREKEPYLSESIIGAVYYPKDGHVIAPELTK
AFAHSAAISGADIYEQTEVFDIRIENNKVTGVITSEGIVTCEKVVIAGGSWSTKLLSYFHRDWGTYPVKGEVVAVRSRKQ
LLKAPIFQERFYITPKRGGRYVIGATMKPHTFNKTVQPESITSILERAYTILPALKEAEWESTWAGLRPQSNHEAPYMGE
HEEIKGLYACTGHYRNGILLSPISGQYMADLIEGKQENHLLDSLLSRRV
>Mature_369_residues
MCEKYDVAIIGGGVIGSSIAHFLAERGHKVAIVEKQSIASEASKAAAGLLGVQAEWDAYDPLFELARESRAIFPQLAAVL
REKTGVDIGYEEKGIYRIAQNEDEKERILHIMDWQQKTGEDSYFLTGDHVREKEPYLSESIIGAVYYPKDGHVIAPELTK
AFAHSAAISGADIYEQTEVFDIRIENNKVTGVITSEGIVTCEKVVIAGGSWSTKLLSYFHRDWGTYPVKGEVVAVRSRKQ
LLKAPIFQERFYITPKRGGRYVIGATMKPHTFNKTVQPESITSILERAYTILPALKEAEWESTWAGLRPQSNHEAPYMGE
HEEIKGLYACTGHYRNGILLSPISGQYMADLIEGKQENHLLDSLLSRRV

Specific function: Catalyzes the FAD-dependent oxidative deamination of various amines and D-amino acids to yield the corresponding alpha- keto acids, ammonia/amine, and hydrogen peroxide. Oxidizes sarcosine (N-methylglycine), N-ethylglycine and glycine. Can also oxidize th

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DAO family [H]

Homologues:

Organism=Homo sapiens, GI24797151, Length=381, Percent_Identity=23.8845144356955, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI194306651, Length=211, Percent_Identity=27.4881516587678, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI197927446, Length=383, Percent_Identity=22.976501305483, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI21361378, Length=383, Percent_Identity=22.976501305483, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1787438, Length=405, Percent_Identity=24.6913580246914, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71994045, Length=396, Percent_Identity=24.2424242424242, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI32563613, Length=389, Percent_Identity=24.9357326478149, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71994052, Length=403, Percent_Identity=23.8213399503722, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20130091, Length=364, Percent_Identity=25.5494505494505, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.3.19 [H]

Molecular weight: Translated: 41206; Mature: 41206

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCEKYDVAIIGGGVIGSSIAHFLAERGHKVAIVEKQSIASEASKAAAGLLGVQAEWDAYD
CCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHEEECCCCCCCC
PLFELARESRAIFPQLAAVLREKTGVDIGYEEKGIYRIAQNEDEKERILHIMDWQQKTGE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHCHHHHCCC
DSYFLTGDHVREKEPYLSESIIGAVYYPKDGHVIAPELTKAFAHSAAISGADIYEQTEVF
CCEEECCCCCCCCCCCHHHHHEEEEEECCCCCEECHHHHHHHHHHHHCCCCHHHCCCEEE
DIRIENNKVTGVITSEGIVTCEKVVIAGGSWSTKLLSYFHRDWGTYPVKGEVVAVRSRKQ
EEEEECCEEEEEEECCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEHHHHH
LLKAPIFQERFYITPKRGGRYVIGATMKPHTFNKTVQPESITSILERAYTILPALKEAEW
HHHCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
ESTWAGLRPQSNHEAPYMGEHEEIKGLYACTGHYRNGILLSPISGQYMADLIEGKQENHL
HHHHCCCCCCCCCCCCCCCCHHHHCEEEEEECCCCCCEEECCCCCHHHHHHHCCCCHHHH
LDSLLSRRV
HHHHHHHCC
>Mature Secondary Structure
MCEKYDVAIIGGGVIGSSIAHFLAERGHKVAIVEKQSIASEASKAAAGLLGVQAEWDAYD
CCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHEEECCCCCCCC
PLFELARESRAIFPQLAAVLREKTGVDIGYEEKGIYRIAQNEDEKERILHIMDWQQKTGE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHCHHHHCCC
DSYFLTGDHVREKEPYLSESIIGAVYYPKDGHVIAPELTKAFAHSAAISGADIYEQTEVF
CCEEECCCCCCCCCCCHHHHHEEEEEECCCCCEECHHHHHHHHHHHHCCCCHHHCCCEEE
DIRIENNKVTGVITSEGIVTCEKVVIAGGSWSTKLLSYFHRDWGTYPVKGEVVAVRSRKQ
EEEEECCEEEEEEECCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEHHHHH
LLKAPIFQERFYITPKRGGRYVIGATMKPHTFNKTVQPESITSILERAYTILPALKEAEW
HHHCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
ESTWAGLRPQSNHEAPYMGEHEEIKGLYACTGHYRNGILLSPISGQYMADLIEGKQENHL
HHHHCCCCCCCCCCCCCCCCHHHHCEEEEEECCCCCCEEECCCCCHHHHHHHCCCCHHHH
LDSLLSRRV
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9827558; 11744710 [H]