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The map label for this gene is cobB [H]

Identifier: 218234993

GI number: 218234993

Start: 2955785

End: 2956513

Strand: Reverse

Name: cobB [H]

Synonym: BCB4264_A3082

Alternate gene names: 218234993

Gene position: 2956513-2955785 (Counterclockwise)

Preceding gene: 218235245

Following gene: 218230767

Centisome position: 54.56

GC content: 35.25

Gene sequence:

>729_bases
GTGCAACAATTTGAAGAAGTACGTTCAATTTTAGAAAAAGCGAAGAAAATTACAGTATTAACAGGCGCTGGTGCAAGTAC
GGAAAGTGGTATTCCAGATTTTCGTTCAGCAAATGGATTGTATGCTGATGCGAATGTGGAGATGTACTTATCAAGAGGGT
ACTATAACCGAAGTCCGAAAGAATTTTGGAAGCATTATAAAGAAATCTTTCAAATTAATACGTTCCATCAATATAAACCA
AATCGTGGCCATCGCTTTTTAGCTGAGCTAGAAGAACAAGGGAAAGATATTACGATTTTAACGCAAAATATTGACGGTTT
ACATCAATTAGGTGGCAGTAAGCATGTCATTGATTTACATGGAACACTTCAAACAGCACATTGTCCAAAATGTAAATCAG
GCTATGATTTACAGTATATGATTGATCATGAAGTACCGCGTTGTGAGAAGTGTAATTTCATTTTAAATCCAGACGTTGTT
TTATACGGAGATACACTGCCTCAATATCAAAATGCGATGAAACGTTTATATGAAACAGATGTACTTCTCGTTATGGGAAC
GTCGTTAAAAGTACAACCTGTCGCTTCATTCCCACAAATCGCAAAGAGGGAAATCGGGGCAACGACAATTTTAGTAAATG
AAGAGTTAACAGGACAAGAATATAACTTTGATTTTGTTTTTCAAAATAAGATTGGTGAGTTTGTTGAAGGGTTATCTTCA
ATAAAATAA

Upstream 100 bases:

>100_bases
AGAGGTTGGGGGCGCAACGCATTAAGTAAAATGAATTGTACAACACCTCTTGTAAACAACTGGTATAATAAGAATAGGAA
AATACATAGAGGTGATTTTT

Downstream 100 bases:

>100_bases
ATTATCGAAAAAACTGAGGTATCCACCTCAGTTTTTTATTTGGGTAATTGCTGTATTAAGCAACATGAAAACAGGTCGAT
AATGTAGTAACCACATCTCT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MQQFEEVRSILEKAKKITVLTGAGASTESGIPDFRSANGLYADANVEMYLSRGYYNRSPKEFWKHYKEIFQINTFHQYKP
NRGHRFLAELEEQGKDITILTQNIDGLHQLGGSKHVIDLHGTLQTAHCPKCKSGYDLQYMIDHEVPRCEKCNFILNPDVV
LYGDTLPQYQNAMKRLYETDVLLVMGTSLKVQPVASFPQIAKREIGATTILVNEELTGQEYNFDFVFQNKIGEFVEGLSS
IK

Sequences:

>Translated_242_residues
MQQFEEVRSILEKAKKITVLTGAGASTESGIPDFRSANGLYADANVEMYLSRGYYNRSPKEFWKHYKEIFQINTFHQYKP
NRGHRFLAELEEQGKDITILTQNIDGLHQLGGSKHVIDLHGTLQTAHCPKCKSGYDLQYMIDHEVPRCEKCNFILNPDVV
LYGDTLPQYQNAMKRLYETDVLLVMGTSLKVQPVASFPQIAKREIGATTILVNEELTGQEYNFDFVFQNKIGEFVEGLSS
IK
>Mature_242_residues
MQQFEEVRSILEKAKKITVLTGAGASTESGIPDFRSANGLYADANVEMYLSRGYYNRSPKEFWKHYKEIFQINTFHQYKP
NRGHRFLAELEEQGKDITILTQNIDGLHQLGGSKHVIDLHGTLQTAHCPKCKSGYDLQYMIDHEVPRCEKCNFILNPDVV
LYGDTLPQYQNAMKRLYETDVLLVMGTSLKVQPVASFPQIAKREIGATTILVNEELTGQEYNFDFVFQNKIGEFVEGLSS
IK

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI7657575, Length=234, Percent_Identity=37.1794871794872, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI13775602, Length=231, Percent_Identity=36.7965367965368, Blast_Score=137, Evalue=6e-33,
Organism=Homo sapiens, GI13775600, Length=231, Percent_Identity=36.7965367965368, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI6912660, Length=242, Percent_Identity=33.0578512396694, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI6912664, Length=257, Percent_Identity=31.9066147859922, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI63054862, Length=213, Percent_Identity=35.2112676056338, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI300795542, Length=239, Percent_Identity=33.4728033472803, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI13787215, Length=249, Percent_Identity=31.3253012048193, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI300797705, Length=203, Percent_Identity=35.9605911330049, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI215982798, Length=173, Percent_Identity=36.9942196531792, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI6912662, Length=226, Percent_Identity=30.9734513274336, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI300797577, Length=254, Percent_Identity=25.5905511811024, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI308199517, Length=232, Percent_Identity=33.6206896551724, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17541892, Length=231, Percent_Identity=33.7662337662338, Blast_Score=131, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17567771, Length=227, Percent_Identity=27.7533039647577, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71990482, Length=222, Percent_Identity=28.8288288288288, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71990487, Length=224, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324599, Length=280, Percent_Identity=29.6428571428571, Blast_Score=104, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324504, Length=284, Percent_Identity=27.4647887323944, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6325242, Length=258, Percent_Identity=27.906976744186, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320163, Length=254, Percent_Identity=26.7716535433071, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320397, Length=237, Percent_Identity=26.1603375527426, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17137536, Length=252, Percent_Identity=34.5238095238095, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24648389, Length=222, Percent_Identity=33.7837837837838, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28571445, Length=186, Percent_Identity=30.1075268817204, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24645650, Length=248, Percent_Identity=23.3870967741935, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27592; Mature: 27592

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQFEEVRSILEKAKKITVLTGAGASTESGIPDFRSANGLYADANVEMYLSRGYYNRSPK
CCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCHH
EFWKHYKEIFQINTFHQYKPNRGHRFLAELEEQGKDITILTQNIDGLHQLGGSKHVIDLH
HHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCEEEEEC
GTLQTAHCPKCKSGYDLQYMIDHEVPRCEKCNFILNPDVVLYGDTLPQYQNAMKRLYETD
CCEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCEEEECCCCHHHHHHHHHHHHCC
VLLVMGTSLKVQPVASFPQIAKREIGATTILVNEELTGQEYNFDFVFQNKIGEFVEGLSS
EEEEECCCEEEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHH
IK
CC
>Mature Secondary Structure
MQQFEEVRSILEKAKKITVLTGAGASTESGIPDFRSANGLYADANVEMYLSRGYYNRSPK
CCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCHH
EFWKHYKEIFQINTFHQYKPNRGHRFLAELEEQGKDITILTQNIDGLHQLGGSKHVIDLH
HHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCEEEEEC
GTLQTAHCPKCKSGYDLQYMIDHEVPRCEKCNFILNPDVVLYGDTLPQYQNAMKRLYETD
CCEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCEEEECCCCHHHHHHHHHHHHCC
VLLVMGTSLKVQPVASFPQIAKREIGATTILVNEELTGQEYNFDFVFQNKIGEFVEGLSS
EEEEECCCEEEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHH
IK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]