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The map label for this gene is asnO1 [H]

Identifier: 218234964

GI number: 218234964

Start: 1173179

End: 1175026

Strand: Direct

Name: asnO1 [H]

Synonym: BCB4264_A1207

Alternate gene names: 218234964

Gene position: 1173179-1175026 (Clockwise)

Preceding gene: 218231842

Following gene: 218235760

Centisome position: 21.65

GC content: 37.99

Gene sequence:

>1848_bases
ATGTGCGGGATTACAGGATGGGTGGATTATAAACGCTCATTAGAAGGAGAAAGAGATGTCGTTACGAAGATGGCTGAGAC
GTTAGCGAAACGGGGGCCGGATGATAATAAAGTTTGGATTAAAGGAAATGTCGCATTTGGGCATAAGCGGTTAATCGTTG
TGGATCCTGAGGGCGGTAAACAGCCGATGACTTGTTTAAAAGATGAAACGAATTACGCCATTTGCTATAACGGTGAACTT
TATAACACAGAAGACATTCGAAAGGAATTATTAAGAAGAGGATATACGTTCAAAGGTCATTCTGATACAGAAGTATTATT
AGCTTCTTATATTGAATGGAAAGAAGAATGTGTCGATCATTTAAACGGTATATATGCGTTTGCAGTATGGGACGAACAGA
AAGAGCAAGTATTTATTGCGAGAGATCGATTAGGTGTAAAGCCACTTTTTTATAAATATGATAGCGGACGCTTACTATTT
GGTTCGGAGTTAAAAGCGATATTGTCGCATCCAGATGTGAAGGCGGAAGTAACGTTAGAAGGATTATCAGAAATATTCGG
GCTCGGACCGTCAAGAACGCCTGGCCACGGTATTTATGCTGGTATAAAAGAATTGCGTCCAGGTCATGCGATGACATTTT
CAAAGAACGGTTTATGCATATGGAGATATTGGAATGTAGAAAGTAAAAAACATGAAGACTCTTTTGAAGAAACAGTAGAG
AAAACGCGCTTTTTATTACAAGATGCTATTACAAGACAGCTTGTTTCTGATGTACCACTATGTACTTTTTTATCGGGTGG
TGTAGATTCGAGCGCTATTACAGCTATTGCAGCGAAAGAATATGAGAGATTGGGAAAAGGGCAATTACACACGTATTCTA
TTGATTACGAAGATAATGACAAATACTTTAAAGCGAATGCATTCCAGCCAAATTCAGATGCTCCGTTTATTAATTTAATG
ACTGAGACATTTCAAACAACCCATCATCGCTGCGTCATTTCGAATGAAAAATTAGCAGAGTATTTAACTGAAGCAGTACT
CGTTCGTGATTTGCCTGGTATGGCAGATATCGATTCGTCATTATTATGGTTTTGCCGAGAAATTAAACAAGATTTTGTCG
TTGGCTTATCTGGAGAATGTGCAGATGAAATATTTGGTGGATATCCGTGGTTTTATAGAGAAGATGATTTACAATCGAGT
GCATTTCCGTGGATGCGTTCTACAGAGGCACGTGAACAACTTCTAAAAAAAGAATGGAGAAATAAATTAAATTTACAACA
ATATGTACAAAGGCGCTATGAAGAGTCGATTCAAGAAGTTCCTATTTTAGAGGGGGAAAGTCCGCTAGAAGCAAAAAGAC
GCCAATTATTTTATTTAAATATGGTATGGTTTATGACAACATTATTAGACAGAAAAGACCGCATGAGTATGGGAGCGAGC
TTAGAAGTACGTGTTCCATTTGCAGATCATCGTCTTGTCGAATATGCGTGGAATATTCCTTGGGAAATGAAAATGTATAA
AAACCGCGAAAAAGGTCTATTACGTAAAGCGTTAGAAGGTGTACTTCCACATGACATCTTATATAGAAAGAAGAGTCCTT
ATCCGAAAACGCACAATCCGCACTATACAAAAGCAGTAACAGTATGGCTTCAAAATCTATTAACGGATAAAGGTTCAATT
TTACACGAACTGTTTGATAAAGAGCAGTTGAGCGGATTAATTCAGTCTGGCGGCAGTGCATTTCAAACACCATGGTTTGG
TCAATTGATGACTGGGCCACAGCTTTTAGCTCATTTAGCACAAATTCACGTTTGGTTTAAAGAATATGGGGTAAATATTA
AGGAATAA

Upstream 100 bases:

>100_bases
ATAGTAGGTTATCGTCAAGTTTTTCATTTTTTGATCCGCACTCTTAGGGTGGGGATTACTGCACGTTCAAGAAAAGAAAT
AAGATGGAAGGATGATTTAT

Downstream 100 bases:

>100_bases
GGGAAAAGCGGCTTACAATTATGTAAGTCGCTTTTTATTAAAGAAATTTTCATATAAAATTCAAAATCACCTTATAAATA
ATATTTAATTAGAATTATTA

Product: asparagine synthetase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MCGITGWVDYKRSLEGERDVVTKMAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDETNYAICYNGEL
YNTEDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLF
GSELKAILSHPDVKAEVTLEGLSEIFGLGPSRTPGHGIYAGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVE
KTRFLLQDAITRQLVSDVPLCTFLSGGVDSSAITAIAAKEYERLGKGQLHTYSIDYEDNDKYFKANAFQPNSDAPFINLM
TETFQTTHHRCVISNEKLAEYLTEAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSS
AFPWMRSTEAREQLLKKEWRNKLNLQQYVQRRYEESIQEVPILEGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGAS
LEVRVPFADHRLVEYAWNIPWEMKMYKNREKGLLRKALEGVLPHDILYRKKSPYPKTHNPHYTKAVTVWLQNLLTDKGSI
LHELFDKEQLSGLIQSGGSAFQTPWFGQLMTGPQLLAHLAQIHVWFKEYGVNIKE

Sequences:

>Translated_615_residues
MCGITGWVDYKRSLEGERDVVTKMAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDETNYAICYNGEL
YNTEDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLF
GSELKAILSHPDVKAEVTLEGLSEIFGLGPSRTPGHGIYAGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVE
KTRFLLQDAITRQLVSDVPLCTFLSGGVDSSAITAIAAKEYERLGKGQLHTYSIDYEDNDKYFKANAFQPNSDAPFINLM
TETFQTTHHRCVISNEKLAEYLTEAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSS
AFPWMRSTEAREQLLKKEWRNKLNLQQYVQRRYEESIQEVPILEGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGAS
LEVRVPFADHRLVEYAWNIPWEMKMYKNREKGLLRKALEGVLPHDILYRKKSPYPKTHNPHYTKAVTVWLQNLLTDKGSI
LHELFDKEQLSGLIQSGGSAFQTPWFGQLMTGPQLLAHLAQIHVWFKEYGVNIKE
>Mature_615_residues
MCGITGWVDYKRSLEGERDVVTKMAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDETNYAICYNGEL
YNTEDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLF
GSELKAILSHPDVKAEVTLEGLSEIFGLGPSRTPGHGIYAGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVE
KTRFLLQDAITRQLVSDVPLCTFLSGGVDSSAITAIAAKEYERLGKGQLHTYSIDYEDNDKYFKANAFQPNSDAPFINLM
TETFQTTHHRCVISNEKLAEYLTEAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSS
AFPWMRSTEAREQLLKKEWRNKLNLQQYVQRRYEESIQEVPILEGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGAS
LEVRVPFADHRLVEYAWNIPWEMKMYKNREKGLLRKALEGVLPHDILYRKKSPYPKTHNPHYTKAVTVWLQNLLTDKGSI
LHELFDKEQLSGLIQSGGSAFQTPWFGQLMTGPQLLAHLAQIHVWFKEYGVNIKE

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=555, Percent_Identity=25.9459459459459, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI168229252, Length=555, Percent_Identity=25.9459459459459, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI168229250, Length=555, Percent_Identity=25.9459459459459, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI296010848, Length=525, Percent_Identity=25.9047619047619, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI296010852, Length=470, Percent_Identity=23.1914893617021, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI296010850, Length=470, Percent_Identity=23.1914893617021, Blast_Score=100, Evalue=7e-21,
Organism=Escherichia coli, GI1786889, Length=557, Percent_Identity=26.2118491921005, Blast_Score=155, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17560178, Length=584, Percent_Identity=25.513698630137, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71993933, Length=583, Percent_Identity=25.9005145797599, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25147557, Length=583, Percent_Identity=25.9005145797599, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25147560, Length=346, Percent_Identity=27.1676300578035, Blast_Score=96, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6321563, Length=569, Percent_Identity=24.6045694200351, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6325403, Length=572, Percent_Identity=25.1748251748252, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI45553209, Length=538, Percent_Identity=24.7211895910781, Blast_Score=125, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 71023; Mature: 71023

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGWVDYKRSLEGERDVVTKMAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGK
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCEEEEEECCCCCC
QPMTCLKDETNYAICYNGELYNTEDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVDH
CCCHHCCCCCCEEEEECCCEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLFGSELKAILSHPDVKAEVTLE
CCCEEEEEEECCCCCEEEEECCCCCCCEEEEEECCCCEEEHHHHHHHHCCCCCEEEEEHH
GLSEIFGLGPSRTPGHGIYAGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVE
HHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHHH
KTRFLLQDAITRQLVSDVPLCTFLSGGVDSSAITAIAAKEYERLGKGQLHTYSIDYEDND
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
KYFKANAFQPNSDAPFINLMTETFQTTHHRCVISNEKLAEYLTEAVLVRDLPGMADIDSS
CEEEECCCCCCCCCCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHCCCCCCCCHHH
LLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWR
HHHHHHHHHHHHEECCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
NKLNLQQYVQRRYEESIQEVPILEGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGAS
HCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCE
LEVRVPFADHRLVEYAWNIPWEMKMYKNREKGLLRKALEGVLPHDILYRKKSPYPKTHNP
EEEECCCCCCHHHHHHHCCCEEHHHHHCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCC
HYTKAVTVWLQNLLTDKGSILHELFDKEQLSGLIQSGGSAFQTPWFGQLMTGPQLLAHLA
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHH
QIHVWFKEYGVNIKE
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MCGITGWVDYKRSLEGERDVVTKMAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGK
CCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCEEEEEECCCCCC
QPMTCLKDETNYAICYNGELYNTEDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVDH
CCCHHCCCCCCEEEEECCCEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLFGSELKAILSHPDVKAEVTLE
CCCEEEEEEECCCCCEEEEECCCCCCCEEEEEECCCCEEEHHHHHHHHCCCCCEEEEEHH
GLSEIFGLGPSRTPGHGIYAGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVE
HHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHHH
KTRFLLQDAITRQLVSDVPLCTFLSGGVDSSAITAIAAKEYERLGKGQLHTYSIDYEDND
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
KYFKANAFQPNSDAPFINLMTETFQTTHHRCVISNEKLAEYLTEAVLVRDLPGMADIDSS
CEEEECCCCCCCCCCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHCCCCCCCCHHH
LLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWR
HHHHHHHHHHHHEECCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
NKLNLQQYVQRRYEESIQEVPILEGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGAS
HCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCE
LEVRVPFADHRLVEYAWNIPWEMKMYKNREKGLLRKALEGVLPHDILYRKKSPYPKTHNP
EEEECCCCCCHHHHHHHCCCEEHHHHHCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCC
HYTKAVTVWLQNLLTDKGSILHELFDKEQLSGLIQSGGSAFQTPWFGQLMTGPQLLAHLA
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHH
QIHVWFKEYGVNIKE
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]