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The map label for this gene is ponA [H]

Identifier: 218234736

GI number: 218234736

Start: 1529947

End: 1532640

Strand: Reverse

Name: ponA [H]

Synonym: BCB4264_A1605

Alternate gene names: 218234736

Gene position: 1532640-1529947 (Counterclockwise)

Preceding gene: 218235219

Following gene: 218231012

Centisome position: 28.28

GC content: 38.08

Gene sequence:

>2694_bases
ATGTCAGATAATTATCGTTCTCGTACAGAACGAAATCATGTGAAAAATCAAGAAAAAAAAACACATAAAGAAGAAAAACC
AAAGAAAAAAGGCTCCTTTTTCAAGAAATTTCTTATAGGTTGTCTACTTCTTGGTATCGTTGGTCTTGTAGCCGGCGTTT
CCGCTTTCTTTGTTATGGTAAAGGATGCTCCAAAATTAGACAAAGCCAAACTTGTTAATCCTTTATCAACAAAGTTTCTT
GATAAAAACGGGAATTTCTTCTATGAATACGGTGCTGAAAAACGAACACACGTTACTTATGATCAAATTCCAAAAGTAGT
TGAAAATGCATTCCTTGCAACTGAGGATTCGCGTTTCTATGATCATAATGGAATTGATTTTAAACGAACTACAAAAGCAG
TTATGGAAAATGTCACTGGTGGTTTCGGTTCCCAAGGTGGTAGTACGATTACACAGCAAGTCGTTAAAAACTACTTTCTA
ACGATGGACAAAACTGCTAAAAGAAAAGTACAAGAATGGTACTTATCTTACAAATTAGAGCAACAATACTCTAAACATGA
GATTTTAGAAATGTACTTAAATAAGATTAATTTGGGTAACCGTTCATACGGTATTGCGACAGCAGCGCAAAAATATTATG
GTAAAGATTTAAAAGACTTGCAATTACATGAAGCTGCTATGCTCGCTGGTTTACCCCAAGGTCCAAACATTTATGATCCA
ACAAAAAAGGAAAATGTTGAACGAGCAACAAATCGTCGTAATGTCGTATTATCATTAATGAATCGACATGGTTATATAAC
AAAAGAAGAAATGAATAACGCCGTTAAGATCCCAGTAACTGAGGGCCTTCAACCGTCTTCAGAAGTAACTGAGATGAAGT
ATCAAGCATTTTTAGATGCGGTTGTAAAAGAAGTTGAAAAAGAATATCCTGATGTAAATATCGGTTCAGATGGTTTAACG
ATTCATACAACACTTGATCAAGATGCTCAAGATTACGCTGAAAAAATTATGGATGGTAATCTTATTAAGTATCCAAACGA
TCAATTCCAAGGATCATTCGTATTTATGGATACACAATCTGGAGAAGTTCGAGCAATTGGTGCTGGACGTAAAGAAAATA
AGTCCACTTTCAAAGGTCATAATATGGCAACTGATTTAAAACGCCAAGTTGGTTCAACAATGAAACCAATTTTCGACTAC
GGTCCAGCGATTGAGAATCTTCAATGGTCTACTTACCATCAATTAAATGACTCTGAATATACTTATTCGGATGGCACAAA
GATTAAAAATGCTACAGGGAGTTTTAAAGGTGACGTTTCACTTCGTGAAGCATTGAAGAAGTCATTAAACATCCCGGCTT
TAAAGACAGCTCAAACAGTTGGACTTAATAAATCAAAAGAGTTTGCTGAAGGTTTAGGCATGACATTTGAAAATGGAGTA
CATGAATCTACTGCAATTGGTAGTAACGATAGTTCTCCATTAGCAGTAGCAGGTGCATATGCAACCTTTGGTAATGGTGG
TAACTATAATAAACCACACTTCGTAAAAGAAGTAACCTTCAAGGACGGGAAAAAGAAAAGCTTTAAACCGAAAGAACAAC
GCGCTATGCAAGACTATACAGCTTACATGGTAACTGACGTTCTTCGTGACGTAGTAAAACCTGGTTCTGGTGGTACAGGA
CCAACAGCATATGTTTCAGGTGTTGACGTAGCGGGTAAAACTGGAACGCAAAACTTTGATGCAAAAGATTTAAAGAAATA
CGGCATTCCAGCTGATGCAAACAGGGATAGCTGGTTCGCTGGATATACACCACAATATACGATGGCTGTATGGACGGGTT
ACGTAAAGAATGGTCCAGAAAACTACATTAGTGATCGTTATACTAAAATTGCACAGCAAATGTTCCAAGTAATGATGAGC
AAATTCGCTACAGATAAATCACGCTTTGAACGTCCTTCTTCTGTACAAGAAATAAACGGAGAATTATACGTAAAAGGTGC
GAAAAAAGATGCAATTAAACAAATTAAAGTAGATGCCCCTAGTGGTCTGAATGTCACTTTTGATGGCGCTAGCACAGTTA
CACTAAACTGGTCTGGACCAGCAGAAGTCGATGCATATGCAGCCAGCTATAAAGCAACTGATGGTTCAAGTGGTAGCCTA
TCAATAAGTGGCACGACAGCTACTCTTGGTGGTATTAAACCAGGTGTTACTTACAGCTTCTCTGTAGTAGCGAAAAAAGG
CACTGGAACAAGCCCAGCAGTTGGTGCATCCTTCACTGCGCCTGGCGGAACTCCAGATGCGAAAAAAGCTGAGGAAGAGG
CTAAGAAAAAGGCCGATGAAGAAGCTAAGAAAAAAGCTGAGGAAGAAGCCCAGAAAAAGGCTAATGAAGATAAAGTAAAA
CAAGACGAAGCTAAGAAAAAGGCTGAAGAAGAGGCTAAAAAACAACAAGAACAACAGCAAGAACAACAACGTAAACAGCA
AGAAGAAGCTCAAAAGAAAGCTGATGAGGAAGCTAGAAGAAAAGCTGAAGAAGAAGCTAAGAAAAAAGCTGAAGAAGAAG
CTAAGAAGAAAGCTGAAGAAGAGGCCAAGAAAAAAGCTGAAGAAGAGGCTAAGAAACAACAAGAACAGCAACACCAAAAT
ACCGGAGGAGATACACCTCACGCAGACGGTGCTGTTGTTACAACAGAGTCCTAA

Upstream 100 bases:

>100_bases
TATCCGTGTACTAGACATGGTTTATTTTTCGTGATAGAGTCATCACTAAGGCTCTTTTTTCGACTTTTGGGGGAGAGAAT
GAAAGGTAGGAGAAAGTATA

Downstream 100 bases:

>100_bases
TATACAAAAAAAGAAGTCCGTTTCTCTTAACGAGAAGCGGACTTCTTTTTTGTTATCATTTTCTTTACATACAGCTTTTC
TATTTCGATATACAACTGCG

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP1; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 897; Mature: 896

Protein sequence:

>897_residues
MSDNYRSRTERNHVKNQEKKTHKEEKPKKKGSFFKKFLIGCLLLGIVGLVAGVSAFFVMVKDAPKLDKAKLVNPLSTKFL
DKNGNFFYEYGAEKRTHVTYDQIPKVVENAFLATEDSRFYDHNGIDFKRTTKAVMENVTGGFGSQGGSTITQQVVKNYFL
TMDKTAKRKVQEWYLSYKLEQQYSKHEILEMYLNKINLGNRSYGIATAAQKYYGKDLKDLQLHEAAMLAGLPQGPNIYDP
TKKENVERATNRRNVVLSLMNRHGYITKEEMNNAVKIPVTEGLQPSSEVTEMKYQAFLDAVVKEVEKEYPDVNIGSDGLT
IHTTLDQDAQDYAEKIMDGNLIKYPNDQFQGSFVFMDTQSGEVRAIGAGRKENKSTFKGHNMATDLKRQVGSTMKPIFDY
GPAIENLQWSTYHQLNDSEYTYSDGTKIKNATGSFKGDVSLREALKKSLNIPALKTAQTVGLNKSKEFAEGLGMTFENGV
HESTAIGSNDSSPLAVAGAYATFGNGGNYNKPHFVKEVTFKDGKKKSFKPKEQRAMQDYTAYMVTDVLRDVVKPGSGGTG
PTAYVSGVDVAGKTGTQNFDAKDLKKYGIPADANRDSWFAGYTPQYTMAVWTGYVKNGPENYISDRYTKIAQQMFQVMMS
KFATDKSRFERPSSVQEINGELYVKGAKKDAIKQIKVDAPSGLNVTFDGASTVTLNWSGPAEVDAYAASYKATDGSSGSL
SISGTTATLGGIKPGVTYSFSVVAKKGTGTSPAVGASFTAPGGTPDAKKAEEEAKKKADEEAKKKAEEEAQKKANEDKVK
QDEAKKKAEEEAKKQQEQQQEQQRKQQEEAQKKADEEARRKAEEEAKKKAEEEAKKKAEEEAKKKAEEEAKKQQEQQHQN
TGGDTPHADGAVVTTES

Sequences:

>Translated_897_residues
MSDNYRSRTERNHVKNQEKKTHKEEKPKKKGSFFKKFLIGCLLLGIVGLVAGVSAFFVMVKDAPKLDKAKLVNPLSTKFL
DKNGNFFYEYGAEKRTHVTYDQIPKVVENAFLATEDSRFYDHNGIDFKRTTKAVMENVTGGFGSQGGSTITQQVVKNYFL
TMDKTAKRKVQEWYLSYKLEQQYSKHEILEMYLNKINLGNRSYGIATAAQKYYGKDLKDLQLHEAAMLAGLPQGPNIYDP
TKKENVERATNRRNVVLSLMNRHGYITKEEMNNAVKIPVTEGLQPSSEVTEMKYQAFLDAVVKEVEKEYPDVNIGSDGLT
IHTTLDQDAQDYAEKIMDGNLIKYPNDQFQGSFVFMDTQSGEVRAIGAGRKENKSTFKGHNMATDLKRQVGSTMKPIFDY
GPAIENLQWSTYHQLNDSEYTYSDGTKIKNATGSFKGDVSLREALKKSLNIPALKTAQTVGLNKSKEFAEGLGMTFENGV
HESTAIGSNDSSPLAVAGAYATFGNGGNYNKPHFVKEVTFKDGKKKSFKPKEQRAMQDYTAYMVTDVLRDVVKPGSGGTG
PTAYVSGVDVAGKTGTQNFDAKDLKKYGIPADANRDSWFAGYTPQYTMAVWTGYVKNGPENYISDRYTKIAQQMFQVMMS
KFATDKSRFERPSSVQEINGELYVKGAKKDAIKQIKVDAPSGLNVTFDGASTVTLNWSGPAEVDAYAASYKATDGSSGSL
SISGTTATLGGIKPGVTYSFSVVAKKGTGTSPAVGASFTAPGGTPDAKKAEEEAKKKADEEAKKKAEEEAQKKANEDKVK
QDEAKKKAEEEAKKQQEQQQEQQRKQQEEAQKKADEEARRKAEEEAKKKAEEEAKKKAEEEAKKKAEEEAKKQQEQQHQN
TGGDTPHADGAVVTTES
>Mature_896_residues
SDNYRSRTERNHVKNQEKKTHKEEKPKKKGSFFKKFLIGCLLLGIVGLVAGVSAFFVMVKDAPKLDKAKLVNPLSTKFLD
KNGNFFYEYGAEKRTHVTYDQIPKVVENAFLATEDSRFYDHNGIDFKRTTKAVMENVTGGFGSQGGSTITQQVVKNYFLT
MDKTAKRKVQEWYLSYKLEQQYSKHEILEMYLNKINLGNRSYGIATAAQKYYGKDLKDLQLHEAAMLAGLPQGPNIYDPT
KKENVERATNRRNVVLSLMNRHGYITKEEMNNAVKIPVTEGLQPSSEVTEMKYQAFLDAVVKEVEKEYPDVNIGSDGLTI
HTTLDQDAQDYAEKIMDGNLIKYPNDQFQGSFVFMDTQSGEVRAIGAGRKENKSTFKGHNMATDLKRQVGSTMKPIFDYG
PAIENLQWSTYHQLNDSEYTYSDGTKIKNATGSFKGDVSLREALKKSLNIPALKTAQTVGLNKSKEFAEGLGMTFENGVH
ESTAIGSNDSSPLAVAGAYATFGNGGNYNKPHFVKEVTFKDGKKKSFKPKEQRAMQDYTAYMVTDVLRDVVKPGSGGTGP
TAYVSGVDVAGKTGTQNFDAKDLKKYGIPADANRDSWFAGYTPQYTMAVWTGYVKNGPENYISDRYTKIAQQMFQVMMSK
FATDKSRFERPSSVQEINGELYVKGAKKDAIKQIKVDAPSGLNVTFDGASTVTLNWSGPAEVDAYAASYKATDGSSGSLS
ISGTTATLGGIKPGVTYSFSVVAKKGTGTSPAVGASFTAPGGTPDAKKAEEEAKKKADEEAKKKAEEEAQKKANEDKVKQ
DEAKKKAEEEAKKQQEQQQEQQRKQQEEAQKKADEEARRKAEEEAKKKAEEEAKKKAEEEAKKKAEEEAKKQQEQQHQNT
GGDTPHADGAVVTTES

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein. Note=Probably found all over the whole cell at low concentrations. Also localizes to the division site in vegetative cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 fibronectin type-III domain [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=558, Percent_Identity=30.6451612903226, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI87082258, Length=318, Percent_Identity=38.0503144654088, Blast_Score=210, Evalue=4e-55,
Organism=Escherichia coli, GI1788867, Length=600, Percent_Identity=27, Blast_Score=143, Evalue=5e-35,
Organism=Escherichia coli, GI1789601, Length=204, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR001264
- InterPro:   IPR013783
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00041 fn3; PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 99343; Mature: 99212

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50853 FN3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNYRSRTERNHVKNQEKKTHKEEKPKKKGSFFKKFLIGCLLLGIVGLVAGVSAFFVMV
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KDAPKLDKAKLVNPLSTKFLDKNGNFFYEYGAEKRTHVTYDQIPKVVENAFLATEDSRFY
CCCCCCCHHHHCCHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
DHNGIDFKRTTKAVMENVTGGFGSQGGSTITQQVVKNYFLTMDKTAKRKVQEWYLSYKLE
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
QQYSKHEILEMYLNKINLGNRSYGIATAAQKYYGKDLKDLQLHEAAMLAGLPQGPNIYDP
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCC
TKKENVERATNRRNVVLSLMNRHGYITKEEMNNAVKIPVTEGLQPSSEVTEMKYQAFLDA
CHHHHHHHHHHHHHHHHHHHHHCCCEEHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
VVKEVEKEYPDVNIGSDGLTIHTTLDQDAQDYAEKIMDGNLIKYPNDQFQGSFVFMDTQS
HHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCC
GEVRAIGAGRKENKSTFKGHNMATDLKRQVGSTMKPIFDYGPAIENLQWSTYHQLNDSEY
CCEEEECCCCCCCCCHHCCCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHCCCCCCE
TYSDGTKIKNATGSFKGDVSLREALKKSLNIPALKTAQTVGLNKSKEFAEGLGMTFENGV
EECCCCEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHCCCCHHCCC
HESTAIGSNDSSPLAVAGAYATFGNGGNYNKPHFVKEVTFKDGKKKSFKPKEQRAMQDYT
CCHHCCCCCCCCCEEEEEHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
AYMVTDVLRDVVKPGSGGTGPTAYVSGVDVAGKTGTQNFDAKDLKKYGIPADANRDSWFA
HHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEC
GYTPQYTMAVWTGYVKNGPENYISDRYTKIAQQMFQVMMSKFATDKSRFERPSSVQEING
CCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHCCC
ELYVKGAKKDAIKQIKVDAPSGLNVTFDGASTVTLNWSGPAEVDAYAASYKATDGSSGSL
EEEEECCCHHHHHHEEECCCCCCEEEECCCEEEEEECCCCCCHHHHHHHCCCCCCCCCEE
SISGTTATLGGIKPGVTYSFSVVAKKGTGTSPAVGASFTAPGGTPDAKKAEEEAKKKADE
EECCCEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EAKKKAEEEAQKKANEDKVKQDEAKKKAEEEAKKQQEQQQEQQRKQQEEAQKKADEEARR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAEEEAKKKAEEEAKKKAEEEAKKKAEEEAKKQQEQQHQNTGGDTPHADGAVVTTES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
SDNYRSRTERNHVKNQEKKTHKEEKPKKKGSFFKKFLIGCLLLGIVGLVAGVSAFFVMV
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KDAPKLDKAKLVNPLSTKFLDKNGNFFYEYGAEKRTHVTYDQIPKVVENAFLATEDSRFY
CCCCCCCHHHHCCHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
DHNGIDFKRTTKAVMENVTGGFGSQGGSTITQQVVKNYFLTMDKTAKRKVQEWYLSYKLE
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
QQYSKHEILEMYLNKINLGNRSYGIATAAQKYYGKDLKDLQLHEAAMLAGLPQGPNIYDP
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCC
TKKENVERATNRRNVVLSLMNRHGYITKEEMNNAVKIPVTEGLQPSSEVTEMKYQAFLDA
CHHHHHHHHHHHHHHHHHHHHHCCCEEHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
VVKEVEKEYPDVNIGSDGLTIHTTLDQDAQDYAEKIMDGNLIKYPNDQFQGSFVFMDTQS
HHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCC
GEVRAIGAGRKENKSTFKGHNMATDLKRQVGSTMKPIFDYGPAIENLQWSTYHQLNDSEY
CCEEEECCCCCCCCCHHCCCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHCCCCCCE
TYSDGTKIKNATGSFKGDVSLREALKKSLNIPALKTAQTVGLNKSKEFAEGLGMTFENGV
EECCCCEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHCCCCHHCCC
HESTAIGSNDSSPLAVAGAYATFGNGGNYNKPHFVKEVTFKDGKKKSFKPKEQRAMQDYT
CCHHCCCCCCCCCEEEEEHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
AYMVTDVLRDVVKPGSGGTGPTAYVSGVDVAGKTGTQNFDAKDLKKYGIPADANRDSWFA
HHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEC
GYTPQYTMAVWTGYVKNGPENYISDRYTKIAQQMFQVMMSKFATDKSRFERPSSVQEING
CCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHCCC
ELYVKGAKKDAIKQIKVDAPSGLNVTFDGASTVTLNWSGPAEVDAYAASYKATDGSSGSL
EEEEECCCHHHHHHEEECCCCCCEEEECCCEEEEEECCCCCCHHHHHHHCCCCCCCCCEE
SISGTTATLGGIKPGVTYSFSVVAKKGTGTSPAVGASFTAPGGTPDAKKAEEEAKKKADE
EECCCEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
EAKKKAEEEAQKKANEDKVKQDEAKKKAEEEAKKQQEQQQEQQRKQQEEAQKKADEEARR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAEEEAKKKAEEEAKKKAEEEAKKKAEEEAKKQQEQQHQNTGGDTPHADGAVVTTES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7814321; 8760912; 9384377; 9721295; 10322023 [H]