Click here to switch to the map view.

The map label for this gene is lmrB [H]

Identifier: 218234646

GI number: 218234646

Start: 973863

End: 975338

Strand: Direct

Name: lmrB [H]

Synonym: BCB4264_A0988

Alternate gene names: 218234646

Gene position: 973863-975338 (Clockwise)

Preceding gene: 218230960

Following gene: 218235891

Centisome position: 17.97

GC content: 36.25

Gene sequence:

>1476_bases
ATGAATATGACAACTAAGAGCCCTACAAGTGGCAAAGTTGATACTTCTAATTTGAAACATACCCCTATTTTAATTGCGTT
ACTTTTAGGAGCAATGGTTGCATTATTAAATGAAACATTACTTGGTAATGCGTTAACAGTATTAATGAAAGAATTTGACG
TAACAGCTTCAACGATTCAATGGCTTTCTACAGCTTACATGTTGGTGGTTGGGGTTTTAGTCCCAATTACAGCGTTACTT
CAGCAATGGCTTACAACGAGACAAATGTTTTTGATCGCTATGGTAACGTTTTTAGCTGGTACATTAATTGCGGGATTTGC
ACCAACATTTTCTGTTTTATTAATCGGCCGTATCGTCCAAGCTATAGCGACAGGGTTAATTTCACCGTTATTAATGAATA
CGATTTTAATTATTTGTCCGCCTGAAAAACGTGGTGCGACAATGGGATTAATCGCACTTGTGATGATGGCAGCTCCGGCA
ATTGGTCCTACATTATCTGGAGTAATTGTGGATTCATTAAATTGGCGCTGGTTATTCTATATTGTTATTCCTATCGTGAT
CATTTCCATTATGATTGGGATGAAGTACATTCAAAATGTTTCAGAGTTAACAAGGCCAAAAGTAGATTACCCATCTATCC
TTTTATCGACATTAGGATTTGGCGGATTTGTGTATAGCTTTAGTGCATCAGGTGATTTAGGATGGTCCGATGCGAAAGTA
TATGACGCTTTAATTGTTGGGCTTATCTCATTATGTATTTTTGTCGTTCGACAATTAAAAATTGAGAATCCAATTTTAGA
ATTACGCGCATTTAAAGTTCCGATGTTTACATTATCAGTTGGATTAATTGTCATTGTGATGATGTCGTTATTTTCAACGA
TGACTTTATTACCGATGTTCCTGCAAACAGTTTTACTCGTTACAGCTTTTAAGTCAGGAATCATCATGCTTCCGGGAAGT
ATTATTAGCGCTATAATGGGACCGATTGCAGGTAAACTATTTGATAAATTTAGTCCGAAAGTTATTATCGTTCCCGGCAT
TGTGTTAGTAGGGATTGCAATGTTCTTATTTAAAGGCATTACTCCTGATACATCAATGTTACAAATTATTATTATGCATA
GCGTATTAATGGTTGGACTTATGTTTGTCATGACAGCACAAACATACGGTTTAAATCAATTAACACCAGATTTATATCCG
CATGGAACCGCACTGTTTAATACGTTGCAACAAGTAGCTGGTGCAATTGGTACAGCTATCTTTATTTCGAAAATGTCTTC
AGGAACAACTAAGTATATGGAAAGCTCCGCAAATCCACTGGATCCGGTAGAGAAGTTAAACGGTTTAACTTCAGGATTCC
AAGGTGCATTTGCACTTGGGTTAATCTTTATTATTGTTGCATTTATCGTATCGTTATTCTTAAAAGAGGAGAAAGAAGGA
GTAAAGGAAGCGAGAGTTTTACAGAACGAACATTAA

Upstream 100 bases:

>100_bases
AAATTGATTCTCCCTTTTTAATCTACATATGTATATTATATTACATGTGTTTAGGTATATTTAAAGTTTTTTAAGTAAGA
AACAGGAGGAGAATCTGTAA

Downstream 100 bases:

>100_bases
GGTTTATATAAAAAAGACATCTTTCCCTAGCAAAGATGTCTTTTTCATATTTTTTTCGTAATACTTGTTTTGGTTGTCTT
CATACATAAGAATTGGTATG

Product: drug resistance transporter, EmrB/QacA family

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MNMTTKSPTSGKVDTSNLKHTPILIALLLGAMVALLNETLLGNALTVLMKEFDVTASTIQWLSTAYMLVVGVLVPITALL
QQWLTTRQMFLIAMVTFLAGTLIAGFAPTFSVLLIGRIVQAIATGLISPLLMNTILIICPPEKRGATMGLIALVMMAAPA
IGPTLSGVIVDSLNWRWLFYIVIPIVIISIMIGMKYIQNVSELTRPKVDYPSILLSTLGFGGFVYSFSASGDLGWSDAKV
YDALIVGLISLCIFVVRQLKIENPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMFLQTVLLVTAFKSGIIMLPGS
IISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGITPDTSMLQIIIMHSVLMVGLMFVMTAQTYGLNQLTPDLYP
HGTALFNTLQQVAGAIGTAIFISKMSSGTTKYMESSANPLDPVEKLNGLTSGFQGAFALGLIFIIVAFIVSLFLKEEKEG
VKEARVLQNEH

Sequences:

>Translated_491_residues
MNMTTKSPTSGKVDTSNLKHTPILIALLLGAMVALLNETLLGNALTVLMKEFDVTASTIQWLSTAYMLVVGVLVPITALL
QQWLTTRQMFLIAMVTFLAGTLIAGFAPTFSVLLIGRIVQAIATGLISPLLMNTILIICPPEKRGATMGLIALVMMAAPA
IGPTLSGVIVDSLNWRWLFYIVIPIVIISIMIGMKYIQNVSELTRPKVDYPSILLSTLGFGGFVYSFSASGDLGWSDAKV
YDALIVGLISLCIFVVRQLKIENPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMFLQTVLLVTAFKSGIIMLPGS
IISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGITPDTSMLQIIIMHSVLMVGLMFVMTAQTYGLNQLTPDLYP
HGTALFNTLQQVAGAIGTAIFISKMSSGTTKYMESSANPLDPVEKLNGLTSGFQGAFALGLIFIIVAFIVSLFLKEEKEG
VKEARVLQNEH
>Mature_491_residues
MNMTTKSPTSGKVDTSNLKHTPILIALLLGAMVALLNETLLGNALTVLMKEFDVTASTIQWLSTAYMLVVGVLVPITALL
QQWLTTRQMFLIAMVTFLAGTLIAGFAPTFSVLLIGRIVQAIATGLISPLLMNTILIICPPEKRGATMGLIALVMMAAPA
IGPTLSGVIVDSLNWRWLFYIVIPIVIISIMIGMKYIQNVSELTRPKVDYPSILLSTLGFGGFVYSFSASGDLGWSDAKV
YDALIVGLISLCIFVVRQLKIENPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMFLQTVLLVTAFKSGIIMLPGS
IISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGITPDTSMLQIIIMHSVLMVGLMFVMTAQTYGLNQLTPDLYP
HGTALFNTLQQVAGAIGTAIFISKMSSGTTKYMESSANPLDPVEKLNGLTSGFQGAFALGLIFIIVAFIVSLFLKEEKEG
VKEARVLQNEH

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=426, Percent_Identity=24.8826291079812, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788710, Length=405, Percent_Identity=24.9382716049383, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87081983, Length=333, Percent_Identity=24.024024024024, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790195, Length=390, Percent_Identity=24.3589743589744, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788392, Length=395, Percent_Identity=24.3037974683544, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=318, Percent_Identity=26.4150943396226, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=128, Percent_Identity=29.6875, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=320, Percent_Identity=22.8125, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=136, Percent_Identity=30.1470588235294, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53054; Mature: 53054

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMTTKSPTSGKVDTSNLKHTPILIALLLGAMVALLNETLLGNALTVLMKEFDVTASTIQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
WLSTAYMLVVGVLVPITALLQQWLTTRQMFLIAMVTFLAGTLIAGFAPTFSVLLIGRIVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIATGLISPLLMNTILIICPPEKRGATMGLIALVMMAAPAIGPTLSGVIVDSLNWRWLFY
HHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHH
IVIPIVIISIMIGMKYIQNVSELTRPKVDYPSILLSTLGFGGFVYSFSASGDLGWSDAKV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHEEEECCCCCCCCCHHHH
YDALIVGLISLCIFVVRQLKIENPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMF
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQTVLLVTAFKSGIIMLPGSIISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGI
HHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCC
TPDTSMLQIIIMHSVLMVGLMFVMTAQTYGLNQLTPDLYPHGTALFNTLQQVAGAIGTAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FISKMSSGTTKYMESSANPLDPVEKLNGLTSGFQGAFALGLIFIIVAFIVSLFLKEEKEG
HHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKEARVLQNEH
HHHHHHHCCCC
>Mature Secondary Structure
MNMTTKSPTSGKVDTSNLKHTPILIALLLGAMVALLNETLLGNALTVLMKEFDVTASTIQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
WLSTAYMLVVGVLVPITALLQQWLTTRQMFLIAMVTFLAGTLIAGFAPTFSVLLIGRIVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIATGLISPLLMNTILIICPPEKRGATMGLIALVMMAAPAIGPTLSGVIVDSLNWRWLFY
HHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHH
IVIPIVIISIMIGMKYIQNVSELTRPKVDYPSILLSTLGFGGFVYSFSASGDLGWSDAKV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHEEEECCCCCCCCCHHHH
YDALIVGLISLCIFVVRQLKIENPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMF
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQTVLLVTAFKSGIIMLPGSIISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGI
HHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCC
TPDTSMLQIIIMHSVLMVGLMFVMTAQTYGLNQLTPDLYPHGTALFNTLQQVAGAIGTAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FISKMSSGTTKYMESSANPLDPVEKLNGLTSGFQGAFALGLIFIIVAFIVSLFLKEEKEG
HHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKEARVLQNEH
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]