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The map label for this gene is dnaN1 [H]

Identifier: 218234602

GI number: 218234602

Start: 1926

End: 3071

Strand: Direct

Name: dnaN1 [H]

Synonym: BCB4264_A0002

Alternate gene names: 218234602

Gene position: 1926-3071 (Clockwise)

Preceding gene: 218235327

Following gene: 218233866

Centisome position: 0.04

GC content: 33.25

Gene sequence:

>1146_bases
ATGCGTTTTACAATACAAAAAGACTATCTTGTAAGAGGTGTACAAGATGTAATGAAGGCAGTTTCTTCTCGTACAACAAT
CCCAATTCTTACGGGAATTAAAGTTGTAGCGACTGAAGAAGGAGTTACATTAACAGGAAGCGATGCTGATATTTCTATTG
AATCGTTTATTCCAGTCGAAGAAGATGGAAAAGAAATCGTAGAAATAGCTCAATCAGGAAGCATTGTTTTACAGGCAAAA
TATTTTAGTGAGATTGTAAAAAAACTACCTAAAGACACTGTCGAAATTTCTGTAGAAAATCATTTTATGACAAAAATAAA
ATCTGGAAAATCAGAGTTTAACTTAAATGGTTTAGATTCTGCAGAATATCCGTTATTGCCACAAATTGAAGAACATCATG
TATTTAAGATACCAACAGATTTACTTAAACATATGATCAGACAAACTGTATTTGCAGTTTCTACTTCTGAAACAAGACCA
ATCTTGACAGGTGTAAACTGGAAGGTATATAACAGCGAGTTAACTTGTATTGCAACAGATAGTCATAGGCTGGCACTTCG
AAAAGCAAAAATTGAAGGACATAATATTGCAGATGAATTCCAAGCTAATGTTGTTATTCCAGGTAAGAGCTTAAATGAAT
TAAGTAAAATTTTAGATGAGTCTGAAGAAATGGTAGATATCGTTATTACGGAGTATCAAGTATTATTCCGTACAAAACAT
TTATTATTCTTCTCAAGATTGTTAGAAGGGAATTATCCTGATACGACACGTTTAATTCCGGCAGAGAGTAAAACGGATAT
TTTTGTGAATACAAAAGAATTCCTACAAGCAATTGATCGCGCTTCACTATTAGCAAGAGATGGCCGCAATAACGTTGTTA
AATTATCAACTTTAGAGCAAGAAATGCTAGAGATTTCTTCGAATTCACCAGAAATCGGAAAAGTTGTCGAAGAAGTACAA
TGCGAAAAAGTAGATGGAGAAGAGTTAAAAATATCTTTTAGTGCAAAATATATGATGGATGCATTGAAAGCTTTGGATAG
TACAGAGATTAAAGTTAGCTTTACTGGGGCGATGAGACCGTTCTTAATTCGTACAGTAAATGATGATTCAATTATTCAAT
TAATTTTACCGGTTCGTACTTACTAG

Upstream 100 bases:

>100_bases
AGCGGAGTTATCCACATATCCACAAGCCCTATTACTATTACTACTATTTTTTATCTTTATTAATTAAATAAAATCTTATA
CTTACCGGAGGTTTTTCTTT

Downstream 100 bases:

>100_bases
GTAAGGAATAAGGGTTGCTAGTTTAAAGATGCTAGTGGCCCTTATTTGATTTTTGGGTATTACTTTCCTAATGCTAGTTT
ATTTAGTACAATGAAAGAAT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MRFTIQKDYLVRGVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDADISIESFIPVEEDGKEIVEIAQSGSIVLQAK
YFSEIVKKLPKDTVEISVENHFMTKIKSGKSEFNLNGLDSAEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSTSETRP
ILTGVNWKVYNSELTCIATDSHRLALRKAKIEGHNIADEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKH
LLFFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTLEQEMLEISSNSPEIGKVVEEVQ
CEKVDGEELKISFSAKYMMDALKALDSTEIKVSFTGAMRPFLIRTVNDDSIIQLILPVRTY

Sequences:

>Translated_381_residues
MRFTIQKDYLVRGVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDADISIESFIPVEEDGKEIVEIAQSGSIVLQAK
YFSEIVKKLPKDTVEISVENHFMTKIKSGKSEFNLNGLDSAEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSTSETRP
ILTGVNWKVYNSELTCIATDSHRLALRKAKIEGHNIADEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKH
LLFFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTLEQEMLEISSNSPEIGKVVEEVQ
CEKVDGEELKISFSAKYMMDALKALDSTEIKVSFTGAMRPFLIRTVNDDSIIQLILPVRTY
>Mature_381_residues
MRFTIQKDYLVRGVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDADISIESFIPVEEDGKEIVEIAQSGSIVLQAK
YFSEIVKKLPKDTVEISVENHFMTKIKSGKSEFNLNGLDSAEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSTSETRP
ILTGVNWKVYNSELTCIATDSHRLALRKAKIEGHNIADEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKH
LLFFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTLEQEMLEISSNSPEIGKVVEEVQ
CEKVDGEELKISFSAKYMMDALKALDSTEIKVSFTGAMRPFLIRTVNDDSIIQLILPVRTY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=380, Percent_Identity=25.7894736842105, Blast_Score=152, Evalue=4e-38,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42832; Mature: 42832

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFTIQKDYLVRGVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDADISIESFIPVE
CEEEECHHHHHHHHHHHHHHHHCCCCEEEEECEEEEEECCCEEEECCCCCEEHHHCCCCC
EDGKEIVEIAQSGSIVLQAKYFSEIVKKLPKDTVEISVENHFMTKIKSGKSEFNLNGLDS
CCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCCEEEEEECHHHHHHHHCCCCCCCCCCCCC
AEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSTSETRPILTGVNWKVYNSELTCIATD
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHEEECCCCCCEEECCEEEEECCEEEEEEEC
SHRLALRKAKIEGHNIADEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKH
CCEEEEEEEECCCCCCHHHCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LLFFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTLEQ
HHHHHHHHCCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEHHHHH
EMLEISSNSPEIGKVVEEVQCEKVDGEELKISFSAKYMMDALKALDSTEIKVSFTGAMRP
HHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCC
FLIRTVNDDSIIQLILPVRTY
EEEEEECCCCEEEEEEECCCC
>Mature Secondary Structure
MRFTIQKDYLVRGVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDADISIESFIPVE
CEEEECHHHHHHHHHHHHHHHHCCCCEEEEECEEEEEECCCEEEECCCCCEEHHHCCCCC
EDGKEIVEIAQSGSIVLQAKYFSEIVKKLPKDTVEISVENHFMTKIKSGKSEFNLNGLDS
CCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCCEEEEEECHHHHHHHHCCCCCCCCCCCCC
AEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSTSETRPILTGVNWKVYNSELTCIATD
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHEEECCCCCCEEECCEEEEECCEEEEEEEC
SHRLALRKAKIEGHNIADEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKH
CCEEEEEEEECCCCCCHHHCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LLFFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTLEQ
HHHHHHHHCCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEHHHHH
EMLEISSNSPEIGKVVEEVQCEKVDGEELKISFSAKYMMDALKALDSTEIKVSFTGAMRP
HHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCC
FLIRTVNDDSIIQLILPVRTY
EEEEEECCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]