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The map label for this gene is azoR1 [H]

Identifier: 218234365

GI number: 218234365

Start: 1825875

End: 1826567

Strand: Direct

Name: azoR1 [H]

Synonym: BCB4264_A1914

Alternate gene names: 218234365

Gene position: 1825875-1826567 (Clockwise)

Preceding gene: 218233042

Following gene: 218234976

Centisome position: 33.69

GC content: 34.63

Gene sequence:

>693_bases
ATGGGACTATTTAGCTCATTATTTGGTAAAAAAGAAGAAAATACGAATGTAGAGGGGAATAAAAAAATGTCAAAAGTATT
ATTTGTAAAAGCAAACGATCGTCCAGCGGAGCAAGCAGTTAGTTCAAAAATGTATGAAACATTTGTAAGTACTTATAAAG
AAGCAAATCCAAATACAGAAATTACGGAATTAGATTTATTTGCATTAGATCTTCCTTATTACGGAAATATCGCGATTTCA
GGTGGATATAAACGTAGCCAAGGCATGGAGTTAACTGCTGAAGAAGAGAAGGCAGTTGCTACAGTAGATCAATATTTAAA
TCAGTTTTTAGAAGCTGATAAGGTTGTATTTGCATTCCCATTATGGAACTTTACAGTACCAGCGCCATTAATCACATATA
TTTCATACTTATCTCAAGCTGGAAAAACGTTTAAATATACAGCAAATGGCCCAGAAGGTTTAGTTGGTGGTAAAAAAGTA
GTTGTGTTAGGTGCTCGTGGTTCAGATTACTCTTCAGAGCAAATGGCTCCTATGGAAATGGCAGTTAATTACGTAACAAC
GGTACTTGGATTCTGGGGAATTACAAATCCAGAGACAGTTGTAATTGAAGGACACAATCAATATCCAGATCGTTCACAAC
AAATTGTTGAAGAAGGTCTAGAAAACGTTAAAAAAGTAGCAGCAAAATTTTAA

Upstream 100 bases:

>100_bases
GTGATAGTGTAACTTAAGGTATCTATTGGAACTTTTTCTTAGAATAAGAATTTTTATGATGATGAATAATGAATATGAAA
AGAAAGATTAGGTGAAGAAA

Downstream 100 bases:

>100_bases
TTCATGATAGTAAATGGAAAAACCAACATGGATGACCATGTTGGTTTTTTAGTCTATTTTTCATGTTAAAAAATGTATAT
TTATAAAATAAAAGAAAAGT

Product: azoreductase

Products: NAD+; NADP+; reduced acceptor

Alternate protein names: Azo-dye reductase 1; FMN-dependent NADH-azo compound oxidoreductase 1 [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MGLFSSLFGKKEENTNVEGNKKMSKVLFVKANDRPAEQAVSSKMYETFVSTYKEANPNTEITELDLFALDLPYYGNIAIS
GGYKRSQGMELTAEEEKAVATVDQYLNQFLEADKVVFAFPLWNFTVPAPLITYISYLSQAGKTFKYTANGPEGLVGGKKV
VVLGARGSDYSSEQMAPMEMAVNYVTTVLGFWGITNPETVVIEGHNQYPDRSQQIVEEGLENVKKVAAKF

Sequences:

>Translated_230_residues
MGLFSSLFGKKEENTNVEGNKKMSKVLFVKANDRPAEQAVSSKMYETFVSTYKEANPNTEITELDLFALDLPYYGNIAIS
GGYKRSQGMELTAEEEKAVATVDQYLNQFLEADKVVFAFPLWNFTVPAPLITYISYLSQAGKTFKYTANGPEGLVGGKKV
VVLGARGSDYSSEQMAPMEMAVNYVTTVLGFWGITNPETVVIEGHNQYPDRSQQIVEEGLENVKKVAAKF
>Mature_229_residues
GLFSSLFGKKEENTNVEGNKKMSKVLFVKANDRPAEQAVSSKMYETFVSTYKEANPNTEITELDLFALDLPYYGNIAISG
GYKRSQGMELTAEEEKAVATVDQYLNQFLEADKVVFAFPLWNFTVPAPLITYISYLSQAGKTFKYTANGPEGLVGGKKVV
VLGARGSDYSSEQMAPMEMAVNYVTTVLGFWGITNPETVVIEGHNQYPDRSQQIVEEGLENVKKVAAKF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=182, Percent_Identity=35.1648351648352, Blast_Score=104, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.2

Molecular weight: Translated: 25464; Mature: 25333

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLFSSLFGKKEENTNVEGNKKMSKVLFVKANDRPAEQAVSSKMYETFVSTYKEANPNTE
CCCHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC
ITELDLFALDLPYYGNIAISGGYKRSQGMELTAEEEKAVATVDQYLNQFLEADKVVFAFP
EEEEEEEEECCCCCCCEEEECCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCEEEEEE
LWNFTVPAPLITYISYLSQAGKTFKYTANGPEGLVGGKKVVVLGARGSDYSSEQMAPMEM
CCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHH
AVNYVTTVLGFWGITNPETVVIEGHNQYPDRSQQIVEEGLENVKKVAAKF
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GLFSSLFGKKEENTNVEGNKKMSKVLFVKANDRPAEQAVSSKMYETFVSTYKEANPNTE
CCHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC
ITELDLFALDLPYYGNIAISGGYKRSQGMELTAEEEKAVATVDQYLNQFLEADKVVFAFP
EEEEEEEEECCCCCCCEEEECCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCEEEEEE
LWNFTVPAPLITYISYLSQAGKTFKYTANGPEGLVGGKKVVVLGARGSDYSSEQMAPMEM
CCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHH
AVNYVTTVLGFWGITNPETVVIEGHNQYPDRSQQIVEEGLENVKKVAAKF
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; NADPH; H+; acceptor

Specific reaction: NAD(P)H + H+ + acceptor = NAD(P)+ + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]