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The map label for this gene is ywkD [H]

Identifier: 218234347

GI number: 218234347

Start: 631294

End: 631680

Strand: Direct

Name: ywkD [H]

Synonym: BCB4264_A0645

Alternate gene names: 218234347

Gene position: 631294-631680 (Clockwise)

Preceding gene: 218232253

Following gene: 218233762

Centisome position: 11.65

GC content: 34.63

Gene sequence:

>387_bases
ATGAATATATGTAGAGTGCATCACGTTGCAATTATTTGTTCGAATTATGAGACGTCAAAGGATTTTTATACTAGAATATT
AGGGTTTAAAGAGATAAATGAAGTATATAGAAAGGAACGAGATTCTTATAAATTAGATTTATGTGTAGGAGAAGAGTATC
AAATCGAATTATTTTCATTTCCAAATCCGCCTGAGCGCAAAAGTTTTCCAGAAGCAGCCGGTCTTAGACATTTAGCATTT
GCTGTTACAAATATAGAAGAAGCTGTGAAGCATTTAAACCAATGTGGTGTTGAGACGGAACCGATCCGTATTGATGAAAT
AACGGGGAAGAAATTCGTCTTTTTCCAAGACCCTGATGCTTTACCTTTAGAATTGTATGAGGTTTGA

Upstream 100 bases:

>100_bases
GAATTTCATGGTAAGCCCCCTAAAAGAGATATTCTGCTATATATGTTTTTCATTTCAAGGGAGAATATGCATCTTTGGTA
AAGAAAGGAATAGAGAGAGC

Downstream 100 bases:

>100_bases
AAATTGGTGGATTTTATATAGAGTTGCTGTCCAATAATTGAAGAAACTAAAGTGAAACTTCTTTTTAAAAGGAGGTGTAA
CTTTAGCTAAGAAAGCTAAA

Product: glyoxylase family protein

Products: glutathione; methylglyoxal

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MNICRVHHVAIICSNYETSKDFYTRILGFKEINEVYRKERDSYKLDLCVGEEYQIELFSFPNPPERKSFPEAAGLRHLAF
AVTNIEEAVKHLNQCGVETEPIRIDEITGKKFVFFQDPDALPLELYEV

Sequences:

>Translated_128_residues
MNICRVHHVAIICSNYETSKDFYTRILGFKEINEVYRKERDSYKLDLCVGEEYQIELFSFPNPPERKSFPEAAGLRHLAF
AVTNIEEAVKHLNQCGVETEPIRIDEITGKKFVFFQDPDALPLELYEV
>Mature_128_residues
MNICRVHHVAIICSNYETSKDFYTRILGFKEINEVYRKERDSYKLDLCVGEEYQIELFSFPNPPERKSFPEAAGLRHLAF
AVTNIEEAVKHLNQCGVETEPIRIDEITGKKFVFFQDPDALPLELYEV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081697, Length=127, Percent_Identity=58.2677165354331, Blast_Score=151, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 4.4.1.5

Molecular weight: Translated: 14917; Mature: 14917

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNICRVHHVAIICSNYETSKDFYTRILGFKEINEVYRKERDSYKLDLCVGEEYQIELFSF
CCCEEEEEEEEEECCCCCCHHHHHHHHCHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEC
PNPPERKSFPEAAGLRHLAFAVTNIEEAVKHLNQCGVETEPIRIDEITGKKFVFFQDPDA
CCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCC
LPLELYEV
CCEEEEEC
>Mature Secondary Structure
MNICRVHHVAIICSNYETSKDFYTRILGFKEINEVYRKERDSYKLDLCVGEEYQIELFSF
CCCEEEEEEEEEECCCCCCHHHHHHHHCHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEC
PNPPERKSFPEAAGLRHLAFAVTNIEEAVKHLNQCGVETEPIRIDEITGKKFVFFQDPDA
CCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCC
LPLELYEV
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-S-lactoylglutathione

Specific reaction: (R)-S-lactoylglutathione = glutathione + methylglyoxal

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]