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The map label for this gene is cysK2 [H]

Identifier: 218234233

GI number: 218234233

Start: 1743588

End: 1744505

Strand: Direct

Name: cysK2 [H]

Synonym: BCB4264_A1837

Alternate gene names: 218234233

Gene position: 1743588-1744505 (Clockwise)

Preceding gene: 218235000

Following gene: 218234145

Centisome position: 32.18

GC content: 39.43

Gene sequence:

>918_bases
ATGAAATTGTGTGAAAACGTTACGGAATTAATAGGAGATACACCTGTCGTCCGATTATCTAAATTTATTCCGGAAGACGC
AGCAGATGTGTATGTGAAACTAGAAATGTTTAATCCATCGCGCAGTGTGAAAGATCGTGCTGCTTATAATTTACTTCACG
TTGCAGAAGAGAATGGTCTCATCAAACCAGGAGATACAATTATTGAACCGACAAGCGGGAATACAGGGATTGGTTTAGCG
ATGAATGCAGCAGCTAAAGGGTATAAAGCGATTTTAATTATGCCAGATAATATGTCAAAAGAGCGTATTAATTTATTGAA
AGCATACGGAGCAGAAGTAGTTTTAACACCAGCAGAACAAAGAATGCCAGGAGCAATTGCGAAGGCGTTAGAACTGCAAA
AGCAAATACCGAATAGTTTTATCCCGCAACAATTTGAAAATCCAGCTAATCCGAATATTCACCGTTATACGACTGCACTT
GAAATTTACGAACAAATGGATGGAGAGCTTGATGCTTTTGTTGCAACGGCAGGAACAGGCGGAACGATTACAGGGACTGG
TGAAACGTTAAAAGAGAAATTGCCAAACTTATATATTGCAGTAGTGGAACCGAAAGGATCTCCCGTTTTATCCGGTGGTG
TACCAGGTCCTCATAAACTAGTAGGAACAAGCCCAGGATTTATTCCGAAAAACTTAAATACAGAAGTGTATAACGAAATT
ATTCAAATTGCAGATGAAGAGGCTTTAACGACAATGAGAAACTTAGCTAGACAAGAAGGATTATTAGTTGGGCCATCTTC
TGGAGCATCTGTTTACGCTGCAATCATGATAGCGAAACGCCTAGGCGTTGGTAAAAAAGTTTTATGTATTGCGCCTGATA
CAGGTGAACGTTATTTGAGCATGGGGTTATTTGAATAA

Upstream 100 bases:

>100_bases
CTGCAATTTATTATGCTATTATGTAGTTGTCTACCATTAATTGGACTCACTTCATGGTTACTGCCAAGTGATAAGAAAAC
GATAGAATAGGGGATGCGCT

Downstream 100 bases:

>100_bases
AAAGGATAGAAACACCTTAATAGTGAATAGCTGCTATTAAGGTGTTTGTTTTATGGAATGAAGAGGGGGAGAAGAACAAT
GAAACTATTAAAACCAATCC

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKLCENVTELIGDTPVVRLSKFIPEDAADVYVKLEMFNPSRSVKDRAAYNLLHVAEENGLIKPGDTIIEPTSGNTGIGLA
MNAAAKGYKAILIMPDNMSKERINLLKAYGAEVVLTPAEQRMPGAIAKALELQKQIPNSFIPQQFENPANPNIHRYTTAL
EIYEQMDGELDAFVATAGTGGTITGTGETLKEKLPNLYIAVVEPKGSPVLSGGVPGPHKLVGTSPGFIPKNLNTEVYNEI
IQIADEEALTTMRNLARQEGLLVGPSSGASVYAAIMIAKRLGVGKKVLCIAPDTGERYLSMGLFE

Sequences:

>Translated_305_residues
MKLCENVTELIGDTPVVRLSKFIPEDAADVYVKLEMFNPSRSVKDRAAYNLLHVAEENGLIKPGDTIIEPTSGNTGIGLA
MNAAAKGYKAILIMPDNMSKERINLLKAYGAEVVLTPAEQRMPGAIAKALELQKQIPNSFIPQQFENPANPNIHRYTTAL
EIYEQMDGELDAFVATAGTGGTITGTGETLKEKLPNLYIAVVEPKGSPVLSGGVPGPHKLVGTSPGFIPKNLNTEVYNEI
IQIADEEALTTMRNLARQEGLLVGPSSGASVYAAIMIAKRLGVGKKVLCIAPDTGERYLSMGLFE
>Mature_305_residues
MKLCENVTELIGDTPVVRLSKFIPEDAADVYVKLEMFNPSRSVKDRAAYNLLHVAEENGLIKPGDTIIEPTSGNTGIGLA
MNAAAKGYKAILIMPDNMSKERINLLKAYGAEVVLTPAEQRMPGAIAKALELQKQIPNSFIPQQFENPANPNIHRYTTAL
EIYEQMDGELDAFVATAGTGGTITGTGETLKEKLPNLYIAVVEPKGSPVLSGGVPGPHKLVGTSPGFIPKNLNTEVYNEI
IQIADEEALTTMRNLARQEGLLVGPSSGASVYAAIMIAKRLGVGKKVLCIAPDTGERYLSMGLFE

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=309, Percent_Identity=42.7184466019417, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI295821200, Length=309, Percent_Identity=42.7184466019417, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI4557415, Length=309, Percent_Identity=42.7184466019417, Blast_Score=239, Evalue=2e-63,
Organism=Escherichia coli, GI1788754, Length=313, Percent_Identity=45.6869009584665, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI2367138, Length=296, Percent_Identity=42.2297297297297, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1790207, Length=254, Percent_Identity=26.3779527559055, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17535051, Length=304, Percent_Identity=45.7236842105263, Blast_Score=250, Evalue=8e-67,
Organism=Caenorhabditis elegans, GI115535073, Length=302, Percent_Identity=48.3443708609272, Blast_Score=241, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI17562970, Length=304, Percent_Identity=42.1052631578947, Blast_Score=226, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI32566674, Length=299, Percent_Identity=42.809364548495, Blast_Score=224, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17561720, Length=304, Percent_Identity=40.1315789473684, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17534315, Length=318, Percent_Identity=38.0503144654088, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI32566672, Length=212, Percent_Identity=41.5094339622642, Blast_Score=161, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI25147552, Length=316, Percent_Identity=36.3924050632911, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17561716, Length=116, Percent_Identity=37.0689655172414, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17539800, Length=339, Percent_Identity=25.9587020648968, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321594, Length=316, Percent_Identity=36.7088607594937, Blast_Score=179, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321449, Length=327, Percent_Identity=35.7798165137615, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24643623, Length=309, Percent_Identity=40.1294498381877, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI20129101, Length=309, Percent_Identity=40.1294498381877, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32753; Mature: 32753

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLCENVTELIGDTPVVRLSKFIPEDAADVYVKLEMFNPSRSVKDRAAYNLLHVAEENGL
CCHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHEECCCCCE
IKPGDTIIEPTSGNTGIGLAMNAAAKGYKAILIMPDNMSKERINLLKAYGAEVVLTPAEQ
ECCCCCEEECCCCCCCEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCHHH
RMPGAIAKALELQKQIPNSFIPQQFENPANPNIHRYTTALEIYEQMDGELDAFVATAGTG
HCCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCC
GTITGTGETLKEKLPNLYIAVVEPKGSPVLSGGVPGPHKLVGTSPGFIPKNLNTEVYNEI
CEEECCCHHHHHHCCCEEEEEECCCCCEEEECCCCCCCCEECCCCCCCCCCCCHHHHHHH
IQIADEEALTTMRNLARQEGLLVGPSSGASVYAAIMIAKRLGVGKKVLCIAPDTGERYLS
HHHCCHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHH
MGLFE
CCCCC
>Mature Secondary Structure
MKLCENVTELIGDTPVVRLSKFIPEDAADVYVKLEMFNPSRSVKDRAAYNLLHVAEENGL
CCHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHEECCCCCE
IKPGDTIIEPTSGNTGIGLAMNAAAKGYKAILIMPDNMSKERINLLKAYGAEVVLTPAEQ
ECCCCCEEECCCCCCCEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCHHH
RMPGAIAKALELQKQIPNSFIPQQFENPANPNIHRYTTALEIYEQMDGELDAFVATAGTG
HCCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCC
GTITGTGETLKEKLPNLYIAVVEPKGSPVLSGGVPGPHKLVGTSPGFIPKNLNTEVYNEI
CEEECCCHHHHHHCCCEEEEEECCCCCEEEECCCCCCCCEECCCCCCCCCCCCHHHHHHH
IQIADEEALTTMRNLARQEGLLVGPSSGASVYAAIMIAKRLGVGKKVLCIAPDTGERYLS
HHHCCHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHH
MGLFE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]