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The map label for this gene is thiF [H]

Identifier: 218234160

GI number: 218234160

Start: 3587854

End: 3588870

Strand: Reverse

Name: thiF [H]

Synonym: BCB4264_A3675

Alternate gene names: 218234160

Gene position: 3588870-3587854 (Counterclockwise)

Preceding gene: 218233960

Following gene: 218235121

Centisome position: 66.23

GC content: 35.3

Gene sequence:

>1017_bases
ATGGCTGATCGGTATTCACGACAACAGTTGTTCAAACCAATTGGGAGTAAAGGACAAGAAAAAATTCGAAATAAACATGT
GTTAATTGTAGGGGCAGGCGCATTAGGAAGTGCAAGTGCCGAAAGTTTCGTACGTGCAGGTGTTGGCAAGTTGACGATTA
TCGATCGTGACTACGTTGAATGGAGTAATTTACAAAGGCAACAATTGTACTCTGAACAAGATGCGAGAGAGAAAATGCCG
AAAGCAATCGCTGCAAAAAATCGGCTAGAACAAATTAATTCGGAAGTACAAATACATGCTTTCGTAATGGATGCAACTTC
AGAAAATATGGAAGGTCTATTAAAAAATGTAGATGTAATCATTGATGCAACAGATAATTTTGATATTCGATTTGTAATAA
ATGATTTATCACAAAAACATAATATTCCGTGGGTATATGGTTCTTGCGTTGGATCATATGGTATGAGTTATATAATTATT
CCGCAAGAGACACCGTGTTTACATTGTATGCTGAAAAATATCCCTGTTACAGGTGTGACGTGTGATACTGCTGGGATTAT
TAGCCCGACTGTTCAAATCGTCGCAGCATATCAAGTGGCAGAAGCGCTCAAAATTTTAGTGGAAGATTTCTCAGCAATTA
GAAAAACATTTTTCATGTTTGATATATGGAGTAATCAAAATCATTTTATAAAATTAGGGAAAATAAAAACAGACGCTTGT
CTCTCATGCGGTTTGAATCGAACTTATCCTTATTTATCATACGAAAATCAAACGAAAGCAGCTGTTTTATGCGGAAGAAA
TACAGTTCAAATTAGAACAGTAGACAATAGGAAATACAATTTTGATGATATAGAAAAAGTATTAAAAAAACTGGGGAAAG
TAGATCGGAATCCGTATTTACTATCTTGCCAATTCGATGATTACCGCATCGTTTTTTTTCGGGATGGTCGTGTTTTTATT
CATGGTACAAATGATATTTCGAAAGCGAAACAACTATATTATCGCGTATTCGGTTAA

Upstream 100 bases:

>100_bases
TGTAATTGGAGGAGCTGGGTTTGTTGGTTTTGCTTACTTTGCTTGTTATCAGAAACAACATTCTAATATGAAATAGAGTT
CTTGAGAGGAAGGGTATGTA

Downstream 100 bases:

>100_bases
TAGAGAGGGTGTCAACAATGGAAAGAAGAGTGCCAATTACGGTTGAAGAAGCTGTTCGTAAAGTGATGGGATTTGCTGAT
AAGGGCTTAAAGGAGCTATT

Product: thiamine/molybdopterin biosynthesis MoeB-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MADRYSRQQLFKPIGSKGQEKIRNKHVLIVGAGALGSASAESFVRAGVGKLTIIDRDYVEWSNLQRQQLYSEQDAREKMP
KAIAAKNRLEQINSEVQIHAFVMDATSENMEGLLKNVDVIIDATDNFDIRFVINDLSQKHNIPWVYGSCVGSYGMSYIII
PQETPCLHCMLKNIPVTGVTCDTAGIISPTVQIVAAYQVAEALKILVEDFSAIRKTFFMFDIWSNQNHFIKLGKIKTDAC
LSCGLNRTYPYLSYENQTKAAVLCGRNTVQIRTVDNRKYNFDDIEKVLKKLGKVDRNPYLLSCQFDDYRIVFFRDGRVFI
HGTNDISKAKQLYYRVFG

Sequences:

>Translated_338_residues
MADRYSRQQLFKPIGSKGQEKIRNKHVLIVGAGALGSASAESFVRAGVGKLTIIDRDYVEWSNLQRQQLYSEQDAREKMP
KAIAAKNRLEQINSEVQIHAFVMDATSENMEGLLKNVDVIIDATDNFDIRFVINDLSQKHNIPWVYGSCVGSYGMSYIII
PQETPCLHCMLKNIPVTGVTCDTAGIISPTVQIVAAYQVAEALKILVEDFSAIRKTFFMFDIWSNQNHFIKLGKIKTDAC
LSCGLNRTYPYLSYENQTKAAVLCGRNTVQIRTVDNRKYNFDDIEKVLKKLGKVDRNPYLLSCQFDDYRIVFFRDGRVFI
HGTNDISKAKQLYYRVFG
>Mature_337_residues
ADRYSRQQLFKPIGSKGQEKIRNKHVLIVGAGALGSASAESFVRAGVGKLTIIDRDYVEWSNLQRQQLYSEQDAREKMPK
AIAAKNRLEQINSEVQIHAFVMDATSENMEGLLKNVDVIIDATDNFDIRFVINDLSQKHNIPWVYGSCVGSYGMSYIIIP
QETPCLHCMLKNIPVTGVTCDTAGIISPTVQIVAAYQVAEALKILVEDFSAIRKTFFMFDIWSNQNHFIKLGKIKTDACL
SCGLNRTYPYLSYENQTKAAVLCGRNTVQIRTVDNRKYNFDDIEKVLKKLGKVDRNPYLLSCQFDDYRIVFFRDGRVFIH
GTNDISKAKQLYYRVFG

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=248, Percent_Identity=30.241935483871, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI38045948, Length=160, Percent_Identity=31.25, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI150417996, Length=173, Percent_Identity=29.4797687861272, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI13376212, Length=229, Percent_Identity=29.2576419213974, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI38327032, Length=210, Percent_Identity=29.5238095238095, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787048, Length=245, Percent_Identity=32.2448979591837, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87082356, Length=248, Percent_Identity=33.4677419354839, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17540406, Length=250, Percent_Identity=33.2, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI193203301, Length=168, Percent_Identity=27.3809523809524, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17557754, Length=156, Percent_Identity=27.5641025641026, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=284, Percent_Identity=28.169014084507, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320598, Length=170, Percent_Identity=31.1764705882353, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24582879, Length=242, Percent_Identity=31.8181818181818, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI28573937, Length=181, Percent_Identity=29.8342541436464, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24641311, Length=213, Percent_Identity=30.5164319248826, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 38400; Mature: 38268

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRYSRQQLFKPIGSKGQEKIRNKHVLIVGAGALGSASAESFVRAGVGKLTIIDRDYVE
CCCCHHHHHHHHHHCCCCHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCHH
WSNLQRQQLYSEQDAREKMPKAIAAKNRLEQINSEVQIHAFVMDATSENMEGLLKNVDVI
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHCCCEEE
IDATDNFDIRFVINDLSQKHNIPWVYGSCVGSYGMSYIIIPQETPCLHCMLKNIPVTGVT
EECCCCCEEEEEEECHHHHCCCCEEEHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCEEE
CDTAGIISPTVQIVAAYQVAEALKILVEDFSAIRKTFFMFDIWSNQNHFIKLGKIKTDAC
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEECCCHHHH
LSCGLNRTYPYLSYENQTKAAVLCGRNTVQIRTVDNRKYNFDDIEKVLKKLGKVDRNPYL
HHCCCCCCCCEEEECCCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEE
LSCQFDDYRIVFFRDGRVFIHGTNDISKAKQLYYRVFG
EEEECCCEEEEEEECCEEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ADRYSRQQLFKPIGSKGQEKIRNKHVLIVGAGALGSASAESFVRAGVGKLTIIDRDYVE
CCCHHHHHHHHHHCCCCHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCHH
WSNLQRQQLYSEQDAREKMPKAIAAKNRLEQINSEVQIHAFVMDATSENMEGLLKNVDVI
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHCCCEEE
IDATDNFDIRFVINDLSQKHNIPWVYGSCVGSYGMSYIIIPQETPCLHCMLKNIPVTGVT
EECCCCCEEEEEEECHHHHCCCCEEEHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCEEE
CDTAGIISPTVQIVAAYQVAEALKILVEDFSAIRKTFFMFDIWSNQNHFIKLGKIKTDAC
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEECCCHHHH
LSCGLNRTYPYLSYENQTKAAVLCGRNTVQIRTVDNRKYNFDDIEKVLKKLGKVDRNPYL
HHCCCCCCCCEEEECCCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEE
LSCQFDDYRIVFFRDGRVFIHGTNDISKAKQLYYRVFG
EEEECCCEEEEEEECCEEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]