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The map label for this gene is yfkN [H]

Identifier: 218233988

GI number: 218233988

Start: 2649160

End: 2651118

Strand: Reverse

Name: yfkN [H]

Synonym: BCB4264_A2743

Alternate gene names: 218233988

Gene position: 2651118-2649160 (Counterclockwise)

Preceding gene: 218232928

Following gene: 218234167

Centisome position: 48.92

GC content: 34.86

Gene sequence:

>1959_bases
ATGCAAAAAATGAAATGGAAGAACTATGTATGTTACTTTGTAATTCTATTACTGCTTGGAACGGCAGTAATAGTCAAACC
ATCTATCTCTAAGGCTGAGGAATCTGAAGTTAATATCACATTGTTAGGTACTGCAGATATTCATGGTAGATTTATGCCTT
GGGATTATGCGCTTGATGGAGCAAATATGAGTGGAAGTCTGACACAGCTTTATACGGTTATAAAGAAGGTCCGTCAAGAA
AATCCAAATACCATATTAGTAGATGCTGGGGATACAATTCAAGGAAACTCAGTAGAATTATTCAACGATAAGCCACAATC
GCCAATGATGGTAGCGATGAATACAATGGGATATGACGCCTGGGCATTTGGAAATCATGAATTCAACTTTGGATTAGATA
CATTGAAAAAAGTTAGTGCGCAATATAAGGGAAAAACGTTAGCAGGAAATATTTATAAGGAAAATGGAGAGCGCTTTCTT
CCTGCATATACAATTGTAGAAAAAGGTGGAATTAAAGTTGGGATTATTGGTATGAATACACCAATGATTAGTGATTTTGA
AAAAGGGACAGATCACTTAGATGGTTTAGTGGTGAAAAATCCGGTAGAAGAGACGAAAAAGGCAATTAAGGAATTAGAAG
GTAAAGTAGATGTAATGGTAGGAGTTATGCATATGGGATTAGAAAATGAGAATGGTATCCCTGGTACTGGTGTTCAAGAT
ATTGCAAATGCTTGTCCGGAACTAAGCGCTATTTTTGCGGCTCACATGCATAAACTTGTAAAAAAAGAAGTTGTAAATGG
CGTAATTATTACAGAACCAGATAAATATGGAACACATATTTCACGTATTGACCTTACTTTTACAAAGCAAGATGGGAAAT
TGGCTTTAAAAAATAAAACTGCTACCGCTATACCTGTAAAAAATGCTGATGGAACAACGATATTATCTGATCCTACACTT
GAAGAAACATTAACACCTTTTCACGAATATGCGAGAGGAGATGCAAATGTTGTAGTCGCTCAATTAAAGGGTAGAAATCT
TGTTCCTGAAAATGAAATAAAGGGTATTCCAAGTGTTCAGATTCAAGAAACACCTTTATCAGATTTCTTTCATGAAGTTA
TGCTCTACTACAGTAAAGCAGATGTAGTAGCCCATCAAATTGATAATGATTATGCCCGTTTAGATATAGGTCCTATTAAG
AAAAAAGATATTGCGTACAATTACCAATATGCCTTGGGAGAAATTACAGTATATAAGATTTCTGGAAAAGATTTAAAAGA
CTATATGGAATGGGCAGCAGGATATTTTAACTCATCTCGTGCCGGAGATGTAACCGTTAGCTTTGATAAAACCCGCCGTG
CCTCTAAATACAGTACGAACGATTTCTTTGGAGGCGTAAAATACGAAATTGACTTAACAAAACCATATGGAAGTAGAATT
ACAAATTTACGCTCTATTCGTACAAACAAACCAATCAAAATGAGCGATGCTATGACGCTGGGAATGAATGCATATAGAAT
GGAGGCTCTTCAGGCAAAAGGAGGAGCTTTAGAGGGGCGTAAGTTTGAACAAATTTGGTCATCTAAACAAGAGAATGCAT
TTGGAGAAACAGGTGGGACCATTCGTAACCTTGCTATTACATATTTAAAAGAGGTAAAGAACGGTGTATACACGCCAAAA
GTTATGCGTAATTGGAAAATTACTGGTGTAGATTTACATTCTTCAGAGCATAAGGCGGTAGTTGATCTTGTGAATAAAGG
GATTTTAGAAATTCCAAAAACAGAAGACGGAAAATACACAAATATAGCATCTATAAATACGAAAGACTCAATTTCAAAAG
AAGAGATTATATTACTCTCACAAAAAGCAAATATTAATCCAAATCAGTTTAATCATGTAAAGACAAAAGGTGAATTTTAC
AAAAAACTTAGTAGGATTACTAAAAAATATAAATTATAA

Upstream 100 bases:

>100_bases
AAAATTATCTTTTGTAGTTGAAGTTTCCTGAATAATAAGTTACATTTTTAAAGTTCAAGCAACTTTATGAAAGTACAAAA
AGGGGAGAAATATTATTATT

Downstream 100 bases:

>100_bases
AATTTCTTATAGTAATTGTTATTTTAATAGCATGAAACCCACTCTTAAAATAGTTAAGAGTGGGTTTTTATGTTGTTATT
TAGATGTAAGTATACTTTTA

Product: 2',3'-cyclic-nucleotide 2'-phosphodiesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MQKMKWKNYVCYFVILLLLGTAVIVKPSISKAEESEVNITLLGTADIHGRFMPWDYALDGANMSGSLTQLYTVIKKVRQE
NPNTILVDAGDTIQGNSVELFNDKPQSPMMVAMNTMGYDAWAFGNHEFNFGLDTLKKVSAQYKGKTLAGNIYKENGERFL
PAYTIVEKGGIKVGIIGMNTPMISDFEKGTDHLDGLVVKNPVEETKKAIKELEGKVDVMVGVMHMGLENENGIPGTGVQD
IANACPELSAIFAAHMHKLVKKEVVNGVIITEPDKYGTHISRIDLTFTKQDGKLALKNKTATAIPVKNADGTTILSDPTL
EETLTPFHEYARGDANVVVAQLKGRNLVPENEIKGIPSVQIQETPLSDFFHEVMLYYSKADVVAHQIDNDYARLDIGPIK
KKDIAYNYQYALGEITVYKISGKDLKDYMEWAAGYFNSSRAGDVTVSFDKTRRASKYSTNDFFGGVKYEIDLTKPYGSRI
TNLRSIRTNKPIKMSDAMTLGMNAYRMEALQAKGGALEGRKFEQIWSSKQENAFGETGGTIRNLAITYLKEVKNGVYTPK
VMRNWKITGVDLHSSEHKAVVDLVNKGILEIPKTEDGKYTNIASINTKDSISKEEIILLSQKANINPNQFNHVKTKGEFY
KKLSRITKKYKL

Sequences:

>Translated_652_residues
MQKMKWKNYVCYFVILLLLGTAVIVKPSISKAEESEVNITLLGTADIHGRFMPWDYALDGANMSGSLTQLYTVIKKVRQE
NPNTILVDAGDTIQGNSVELFNDKPQSPMMVAMNTMGYDAWAFGNHEFNFGLDTLKKVSAQYKGKTLAGNIYKENGERFL
PAYTIVEKGGIKVGIIGMNTPMISDFEKGTDHLDGLVVKNPVEETKKAIKELEGKVDVMVGVMHMGLENENGIPGTGVQD
IANACPELSAIFAAHMHKLVKKEVVNGVIITEPDKYGTHISRIDLTFTKQDGKLALKNKTATAIPVKNADGTTILSDPTL
EETLTPFHEYARGDANVVVAQLKGRNLVPENEIKGIPSVQIQETPLSDFFHEVMLYYSKADVVAHQIDNDYARLDIGPIK
KKDIAYNYQYALGEITVYKISGKDLKDYMEWAAGYFNSSRAGDVTVSFDKTRRASKYSTNDFFGGVKYEIDLTKPYGSRI
TNLRSIRTNKPIKMSDAMTLGMNAYRMEALQAKGGALEGRKFEQIWSSKQENAFGETGGTIRNLAITYLKEVKNGVYTPK
VMRNWKITGVDLHSSEHKAVVDLVNKGILEIPKTEDGKYTNIASINTKDSISKEEIILLSQKANINPNQFNHVKTKGEFY
KKLSRITKKYKL
>Mature_652_residues
MQKMKWKNYVCYFVILLLLGTAVIVKPSISKAEESEVNITLLGTADIHGRFMPWDYALDGANMSGSLTQLYTVIKKVRQE
NPNTILVDAGDTIQGNSVELFNDKPQSPMMVAMNTMGYDAWAFGNHEFNFGLDTLKKVSAQYKGKTLAGNIYKENGERFL
PAYTIVEKGGIKVGIIGMNTPMISDFEKGTDHLDGLVVKNPVEETKKAIKELEGKVDVMVGVMHMGLENENGIPGTGVQD
IANACPELSAIFAAHMHKLVKKEVVNGVIITEPDKYGTHISRIDLTFTKQDGKLALKNKTATAIPVKNADGTTILSDPTL
EETLTPFHEYARGDANVVVAQLKGRNLVPENEIKGIPSVQIQETPLSDFFHEVMLYYSKADVVAHQIDNDYARLDIGPIK
KKDIAYNYQYALGEITVYKISGKDLKDYMEWAAGYFNSSRAGDVTVSFDKTRRASKYSTNDFFGGVKYEIDLTKPYGSRI
TNLRSIRTNKPIKMSDAMTLGMNAYRMEALQAKGGALEGRKFEQIWSSKQENAFGETGGTIRNLAITYLKEVKNGVYTPK
VMRNWKITGVDLHSSEHKAVVDLVNKGILEIPKTEDGKYTNIASINTKDSISKEEIILLSQKANINPNQFNHVKTKGEFY
KKLSRITKKYKL

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=230, Percent_Identity=27.3913043478261, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI1790658, Length=627, Percent_Identity=25.3588516746411, Blast_Score=132, Evalue=9e-32,
Organism=Escherichia coli, GI1786687, Length=468, Percent_Identity=26.0683760683761, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 72633; Mature: 72633

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKMKWKNYVCYFVILLLLGTAVIVKPSISKAEESEVNITLLGTADIHGRFMPWDYALDG
CCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECC
ANMSGSLTQLYTVIKKVRQENPNTILVDAGDTIQGNSVELFNDKPQSPMMVAMNTMGYDA
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCEEEEEECCCCEE
WAFGNHEFNFGLDTLKKVSAQYKGKTLAGNIYKENGERFLPAYTIVEKGGIKVGIIGMNT
EEECCCEECCCHHHHHHHHHHHCCCEEECCCCCCCCCEECCEEEEEECCCEEEEEEECCC
PMISDFEKGTDHLDGLVVKNPVEETKKAIKELEGKVDVMVGVMHMGLENENGIPGTGVQD
CCCCCHHCCCHHHCCEEECCCHHHHHHHHHHHCCCHHHEEEHHHHCCCCCCCCCCCCHHH
IANACPELSAIFAAHMHKLVKKEVVNGVIITEPDKYGTHISRIDLTFTKQDGKLALKNKT
HHHHCHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHCCEEEEEEEEEEECCCEEEEECCC
ATAIPVKNADGTTILSDPTLEETLTPFHEYARGDANVVVAQLKGRNLVPENEIKGIPSVQ
EEEEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCCCCE
IQETPLSDFFHEVMLYYSKADVVAHQIDNDYARLDIGPIKKKDIAYNYQYALGEITVYKI
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECEEEEEEE
SGKDLKDYMEWAAGYFNSSRAGDVTVSFDKTRRASKYSTNDFFGGVKYEIDLTKPYGSRI
CCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCHHH
TNLRSIRTNKPIKMSDAMTLGMNAYRMEALQAKGGALEGRKFEQIWSSKQENAFGETGGT
HHHHHHCCCCCEEECCCEEECCCHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCH
IRNLAITYLKEVKNGVYTPKVMRNWKITGVDLHSSEHKAVVDLVNKGILEIPKTEDGKYT
HHHHHHHHHHHHHCCCCCCHHHCCEEEEEEEECCCCCHHHHHHHHCCCEECCCCCCCCEE
NIASINTKDSISKEEIILLSQKANINPNQFNHVKTKGEFYKKLSRITKKYKL
EEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQKMKWKNYVCYFVILLLLGTAVIVKPSISKAEESEVNITLLGTADIHGRFMPWDYALDG
CCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECC
ANMSGSLTQLYTVIKKVRQENPNTILVDAGDTIQGNSVELFNDKPQSPMMVAMNTMGYDA
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCEEEEEECCCCEE
WAFGNHEFNFGLDTLKKVSAQYKGKTLAGNIYKENGERFLPAYTIVEKGGIKVGIIGMNT
EEECCCEECCCHHHHHHHHHHHCCCEEECCCCCCCCCEECCEEEEEECCCEEEEEEECCC
PMISDFEKGTDHLDGLVVKNPVEETKKAIKELEGKVDVMVGVMHMGLENENGIPGTGVQD
CCCCCHHCCCHHHCCEEECCCHHHHHHHHHHHCCCHHHEEEHHHHCCCCCCCCCCCCHHH
IANACPELSAIFAAHMHKLVKKEVVNGVIITEPDKYGTHISRIDLTFTKQDGKLALKNKT
HHHHCHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHCCEEEEEEEEEEECCCEEEEECCC
ATAIPVKNADGTTILSDPTLEETLTPFHEYARGDANVVVAQLKGRNLVPENEIKGIPSVQ
EEEEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCCCCE
IQETPLSDFFHEVMLYYSKADVVAHQIDNDYARLDIGPIKKKDIAYNYQYALGEITVYKI
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECEEEEEEE
SGKDLKDYMEWAAGYFNSSRAGDVTVSFDKTRRASKYSTNDFFGGVKYEIDLTKPYGSRI
CCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCHHH
TNLRSIRTNKPIKMSDAMTLGMNAYRMEALQAKGGALEGRKFEQIWSSKQENAFGETGGT
HHHHHHCCCCCEEECCCEEECCCHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCH
IRNLAITYLKEVKNGVYTPKVMRNWKITGVDLHSSEHKAVVDLVNKGILEIPKTEDGKYT
HHHHHHHHHHHHHCCCCCCHHHCCEEEEEEEECCCCCHHHHHHHHCCCEECCCCCCCCEE
NIASINTKDSISKEEIILLSQKANINPNQFNHVKTKGEFYKKLSRITKKYKL
EEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]